Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,879	A→G	9.5%	D769D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,879	0	A	G	9.5%	149.6 / 2.4	74	D769D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand):  ref base A (34/30);  new base G (4/4);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGT  >  USA300TCH1516_ALE/2774745‑2775018                                                                                                                                        |                                                                                                                                             gtgtCCGAGGCGCGTGCCGGGTCTGTGTCGCTATCTGAGGCTGAGTC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCt                                                                                                                                         <  2:757024/137‑1 (MQ=17) ggagccGAGCCGCTATCCGAGGCTGAGTGGCTATCTGAGTCTGAGTC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCt                                                                                                                                         <  2:286531/136‑1 (MQ=17)    tCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATc                                                                                                                                      <  1:97501/140‑1 (MQ=255)    tCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATc                                                                                                                                      <  1:51500/140‑1 (MQ=255)      cGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                                                                                                    <  1:658255/140‑1 (MQ=255)      cGAGCCGCGATCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                                                                                                    <  1:151032/140‑1 (MQ=255)         ctCGCTCTCCGCGTCGGAGTCCCTGTCTGAGTCTGACTCTGCT‑CCTGCGTCCGAGTCGCTCTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCCCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATctgaat                                                                                                                                 <  2:4814/139‑6 (MQ=11)          tCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTc                                                                                                                                >  1:628160/1‑140 (MQ=255)             cTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTGGCTAACTGTGTCTGAGTGGCTGTCTGAGGCTGAGCGGCTGACTGACACTGAATCGCTAGCTGAGACTGAATAGATATCGGAGTcagg                                                                                                                             >  2:219050/1‑137 (MQ=2)             cTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCACTATCCGCGTCTGAGGCGATGTCTGTCTCTGAATCACTGTCTCACGCCGCGTCACTATATGTATCTGAAGCACTCTCCGAgacccc                                                                                                                             >  2:202096/1‑135 (MQ=9)               aTCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGt                                                                                                                           >  1:624840/1‑140 (MQ=255)               aTCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAATCCGAATCGCTATCTAACTCTGAATCCCTATCGGAGTcggcgt                                                                                                                           >  1:22694/1‑135 (MQ=17)                tCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCTATCtgagtctgagtc                                                                                                                          >  2:517585/1‑140 (MQ=255)                  cGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGCGTCTGAGTCGCTGTCGGAGTCTGAGGCGGTGTCTGACTCCGAGTCGCTAGCTGCATCTGAACCGGTGTCCGGGCCGGAGgcgc                                                                                                                        >  2:602331/1‑140 (MQ=9)                  cGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCGTGCCGCCTCTGAGTTGc                                                                                                                        >  1:101389/1‑140 (MQ=17)                       gcgAGTCGCTACCGGAGCCTGAATC‑GCTGTCTGAGTCTGAGGCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtct                                                                                                                   <  1:266867/138‑1 (MQ=18)                       cTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAGTCTGAATCGCTATCCGAGTCTGAGTCGCTGtct                                                                                                                   >  1:340712/1‑140 (MQ=255)                         gAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGCATCCGAATCGCTATCTGTATCTGCACCGCTACTCGCTTCTGACTCGCTgaccga                                                                                                                 >  1:51015/1‑135 (MQ=18)                           gTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGGCGCTGtctgaat                                                                                                               >  1:700621/1‑140 (MQ=255)                            tCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCGGAGTCGCTGtctgaatc                                                                                                              >  1:540632/1‑140 (MQ=255)                               cTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGCCTCTGACTCGCTATCCGAGTCTGAGTCGCTGtctgaatctga                                                                                                           <  1:524051/140‑1 (MQ=255)                                tATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctgaa                                                                                                          <  1:417721/140‑1 (MQ=255)                                 aTCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTctgaatctgaat                                                                                                         >  1:423580/1‑140 (MQ=255)                                           gAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCCATCCGAGACGGAGTCGCTGTCCGAATCTGAATAGCCGTCTGa                                                                                               >  2:304078/1‑140 (MQ=17)                                             aTC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGt                                                                                             >  1:9710/1‑140 (MQ=255)                                             aTC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGt                                                                                             <  1:604146/140‑1 (MQ=255)                                              tC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTc                                                                                            >  1:436898/1‑140 (MQ=255)                                                 gCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCTGAGTCGCTATCTGAATCTGACTCGCTGTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGGTGCAGGAGTCCg                                                                                          >  2:329036/1‑140 (MQ=14)                                                 gCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCGGAGTCGCAGTCTGAGTcga                                                                                          >  2:52419/1‑138 (MQ=17)                                                 gCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGCGTCCg                                                                                          >  1:428553/1‑140 (MQ=255)                                                     tctgagtctgagTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATc                                                                                      >  1:247286/1‑140 (MQ=255)                                                         agtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTa                                                                                  >  1:535494/1‑140 (MQ=255)                                                           tctgtgtcGCTATCCGAGCCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatc                                                                                <  2:351642/140‑1 (MQ=14)                                                           tctgcgtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatc                                                                                <  1:280048/140‑1 (MQ=255)                                                                 tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctga‑atc                                                                         >  1:446029/1‑140 (MQ=255)                                                                  cGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCGGAATCGCTGTCTGAGTCCGAATCGCTatctga‑atct                                                                        >  1:518646/1‑140 (MQ=255)                                                                    cTATCCGCGTCTCCGTCGCGTTCCAAATCTGAATCACTGTCTGAACCCGAATCGCTATCTAAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctga‑atctga                                                                      <  1:451796/140‑1 (MQ=18)                                                                    cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTGTCTGAATCTGAATCGCTCTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAGCGCCGTCTGAGTGCGAATCGCTgtctga‑ctccgt                                                                      >  2:315137/1‑137 (MQ=14)                                                                        cggagtttgagtttCTTACTAAATCTGAATCACTATCTGAAGCTGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCg                                                                  <  2:53878/138‑1 (MQ=14)                                                                        ccGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGATCGAATCGCTGTCTGGAACTGAAACACTGTCAGAGGCCGAGTCGCTGTCGGAGGCTGACGCGTCATCTGAGTGAGAATCGCTgtgtga‑ttctgatgga                                                                  >  2:296820/1‑136 (MQ=2)                                                                         cGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGc                                                                 >  1:181049/1‑140 (MQ=255)                                                                            gTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTgt                                                              >  2:287647/1‑140 (MQ=255)                                                                            gTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTgt                                                              <  1:747372/140‑1 (MQ=255)                                                                              cTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTGTCTGA‑ATCTGAGTCGCTgtct                                                            >  1:92796/1‑140 (MQ=255)                                                                               tGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTAGCTgtatg                                                           >  1:600704/1‑140 (MQ=255)                                                                                gAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTgtctga                                                          >  1:115947/1‑140 (MQ=255)                                                                                gAGGCGCTGTCTGAATCGGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTgtctga                                                          <  1:164873/140‑1 (MQ=255)                                                                                 aGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGCATCGCTCTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGC‑ATCTGAGTCGCTgtctgag                                                         >  1:253018/1‑140 (MQ=255)                                                                                     gCTGTCTGATTCTGAATCTCTGTCTGTGTCCGTGTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTgtctgagtctg                                                     <  2:586544/140‑1 (MQ=14)                                                                                        gTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTGTCTGA‑ATCTGAGTCGCTgtctgagtctgagt                                                  >  2:279168/1‑138 (MQ=255)                                                                                         tctgaatctgaaTCACTGTCTGAATCCGAATCGCTATCTGCGTCGGGATCGGTATCAGAGTCTGACGCGGTGTCGGACTCGGAGTCGCTCGCTGAGTCGAAGACGCTATCTGC‑ATCTGAATCGCTATCTGAGACTGAATc                                                 >  2:46939/1‑140 (MQ=2)                                                                                           tgcatctgtatcACTGTTTGAGTCGGAATCGCTTTCTGGATCTGAATCGCTATCCGAGTCTGAGTCGCCATCTGAATCTGAGTCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGc                                               <  2:475631/137‑1 (MQ=14)                                                                                           tgagtctgaagcACTGTCTGAGTCCGAATCGCTGTCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGGCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGc                                               <  2:616172/140‑1 (MQ=11)                                                                                           tgaatcggaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGc                                               <  1:457417/140‑1 (MQ=255)                                                                                            gaagctgaatcACTGTCTGGCTCCGATTCGCTATCTGAATCGGGGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGA‑ATCTGAGTCGCTGTCTGAATCTGAATCACt                                              <  2:128755/140‑1 (MQ=11)                                                                                                ctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGctctct                                          >  2:583433/1‑140 (MQ=255)                                                                                                 tgagtcACTGTCTGAGGCCGATGCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGGCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctg                                         <  2:418059/140‑1 (MQ=11)                                                                                                 tgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctg                                         <  1:742749/140‑1 (MQ=255)                                                                                                    gtcgcTGTCTGAATCCGCATCGCTGTCTGAATCTGAATCGCTCTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                      <  2:535494/136‑1 (MQ=255)                                                                                                    atcCCTGTCCGAGTCCGAGTCCCGCTCTGAGCCTGCATCGCCGTCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCCGAGTCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                      <  2:199530/140‑1 (MQ=11)                                                                                                    atcACTGGCTGAGTCCGAATCGGTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTTTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                      <  2:316347/140‑1 (MQ=11)                                                                                                      cccTGTCCGAGTCCGGGTCGCTGTCTGAGTCTGAATCGCTCTCCGAGTCCGAGTCGCTGTCTGGATCTGAATCGCTGTCCGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatct                                    <  2:247286/138‑1 (MQ=14)                                                                                                         tGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGCTTCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                 <  1:324189/140‑1 (MQ=255)                                                                                                           tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                               <  1:304078/140‑1 (MQ=255)                                                                                                              gAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGCGTCTGAATCGCTCTCTGAGTCTGAGTCGCt                            >  1:187798/1‑140 (MQ=255)                                                                                                              gAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCt                            <  1:216441/140‑1 (MQ=255)                                                                                                                 tCCGAATCGCTATCTGATTCTGAATCGCTATCCGGGTCTGACTGCCTGCCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtc                         <  1:517585/140‑1 (MQ=14)                                                                                                                 tCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCtctc                         >  2:647705/1‑140 (MQ=14)                                                                                                                    gAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctga                      >  2:243017/1‑140 (MQ=255)                                                                                                                    gAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTAtctga                      >  2:761121/1‑140 (MQ=255)                                                                                                                      aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagt                    <  1:499574/140‑1 (MQ=255)                                                                                                                      aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCGGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGtctgagt                    >  2:715488/1‑140 (MQ=255)                                                                                                                         gCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctg                 <  1:107710/140‑1 (MQ=255)                                                                                                                           tATCTGCCTCTGCATCGCTATCCGACTCCGAGTCCCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgag               <  1:82378/140‑1 (MQ=14)                                                                                                                             tctgaatctgaaTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGACTCGC‑ACCGCACAACGTATTCGCTGTCTGCGTGTGATGCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc             <  1:760071/140‑1 (MQ=14)                                                                                                                                       aatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgag   <  1:287647/140‑1 (MQ=255)                                                                                                                                        atcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCGGAAGCGCTGTCTGAGTCGGAATGGCTATCTGAGTCGGAGTAGCTTtctgagtcggagttgcagtctgaat  >  2:606495/1‑138 (MQ=14)                                                                                                                                        |                                                                                                                                             GAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATC‑GCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGA‑ATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGT  >  USA300TCH1516_ALE/2774745‑2775018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑