BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,775,053	A→G	16.0%	D711D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,775,053	0	A	G	16.0%	178.4 / 10.1	97	D711D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (38/40); new base G (8/9); total (48/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTGT  >  USA300TCH1516_ALE/2774916‑2775192

tcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                                                                                           <  1:731255/140‑1 (MQ=255)

 cGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatct                                                                                                                                          >  1:521902/1‑140 (MQ=255)

 cGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatct                                                                                                                                          >  1:260175/1‑140 (MQ=255)

 cGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAGCTGAGTCTGAGTCTCTATCTGAATCTGAGTCGCTGTCTGGGTCTGACTCGCTatctgagtct                                                                                                                                          >  2:112124/1‑140 (MQ=14)

   cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga                                                                                                                                        <  1:52419/140‑1 (MQ=255)

   cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga                                                                                                                                        <  1:147991/140‑1 (MQ=255)

    cttccgcgTCCGAGTCGCTTTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                                                                                       <  2:181049/133‑1 (MQ=11)

      tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                                                                     <  1:748809/140‑1 (MQ=255)

       cTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCg                                                                                                                                    >  1:124270/1‑140 (MQ=255)

       cTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCGGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                                                                                                    <  1:329036/140‑1 (MQ=255)

          aGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTg                                                                                                                                 <  1:383324/140‑1 (MQ=255)

                aaTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGACTCTGAGTCGCTgtctgag                                                                                                                           >  1:502317/1‑140 (MQ=255)

                 aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                                                                                                                          >  1:392991/1‑140 (MQ=255)

                 aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                                                                                                                          <  1:243017/140‑1 (MQ=255)

                  tCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtcggagtc                                                                                                                         >  1:334923/1‑140 (MQ=255)

                   cGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGGATGGCTATCTGAGTCTGAGTCGCTctctgagtct                                                                                                                        >  1:95097/1‑140 (MQ=255)

                   cGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtct                                                                                                                        >  1:85441/1‑140 (MQ=255)

                    gcgatctgaatctgaGTCGCGGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                                                                                                                       <  1:461986/137‑1 (MQ=255)

                    gCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                                                                                                                       >  1:722837/1‑140 (MQ=255)

                    gCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCGGTCTGAGTCTGAGTCGCTGCCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                                                                                                                       >  1:622034/1‑140 (MQ=255)

                     ctgtctgaagctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctga                                                                                                                      <  1:279168/137‑1 (MQ=255)

                         ctgaagctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                                                                                                                  <  1:672192/140‑1 (MQ=255)

                           taatctgaGTCGCTGTCTGAGTCTGACTCGCTATCTGACTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgct                                                                                                                <  1:424095/139‑1 (MQ=255)

                              tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtc                                                                                                             <  1:732231/140‑1 (MQ=255)

                              tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtc                                                                                                             <  1:504848/140‑1 (MQ=255)

                              gctgaGTCGCTGTCCGAGTCTGCATCCCTCTCTGAATCTGAGTCGCCATCTGAGTCTGAGTGGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTCTCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtc                                                                                                             <  1:336114/139‑1 (MQ=14)

                               ctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGATGTCTGAGTCCGAGTCGCTGTCTGAGTCTGAATCGCGATGTGAGTCTGAGACGCTGTGAGAGtctgacacgctgtcc                                                                                                            >  2:618249/1‑139 (MQ=14)

                                tgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctg                                                                                                           >  2:242580/1‑140 (MQ=255)

                                 gaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGGTGTCTGAGTCTGAGTAGCTGTCTGAGTGGGCGTCGCTATGTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgccgga                                                                                                          >  2:285846/1‑135 (MQ=14)

                                 gaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctga                                                                                                          >  1:754235/1‑140 (MQ=255)

                                 gaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTGGCTGTCTGAGtctgagtcgctgtgtgt                                                                                                          >  1:734616/1‑139 (MQ=255)

                                 gaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctga                                                                                                          >  2:229843/1‑140 (MQ=255)

                                  aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGGGTCGGAGGCGCTGTCTGAGTCTGAGTCGCGTTCTGCGTCGGAGTGGCTTTCTGAGtatgaatcgcggtctgaa                                                                                                         >  2:485396/1‑140 (MQ=14)

                                    tCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGCGTCTGAATCGCTCTCTGAATCTGAGTCGCTGTGTGAGtctgagtcgccctctgtttt                                                                                                       >  1:98553/1‑136 (MQ=17)

                                      gCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtttgaatctg                                                                                                     >  1:274230/1‑140 (MQ=255)

                                        tgtgtgtgTCTTATTCTCTGTCTTAATGTGAGTCTCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGTATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgag                                                                                                   <  2:22694/140‑1 (MQ=14)

                                          tctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAAGCGCTGTCTGAATCCGAGTCGCTGTCTGAGtctgattctctgtgtgacgctgcgtc                                                                                                 >  2:747916/1‑140 (MQ=14)

                                           ctgagtctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg                                                                                                >  1:410152/1‑140 (MQ=255)

                                              ggtcggaGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg                                                                                             <  2:29447/139‑1 (MQ=255)

                                                tctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtc                                                                                           <  1:120750/140‑1 (MQ=255)

                                                gctggtgcGCTGTCTGAGGGTGAGGCGCTGTGTGAGGCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGtctgagtcgctgtctgaatctgagtcgctgtc                                                                                           <  2:512690/133‑1 (MQ=11)

                                                 ctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtct                                                                                          >  1:127567/1‑140 (MQ=255)

                                                 ctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGACTCGCTATCTGAGTCTGAGTCGCTGTCTGAGtctgagtcgcggtctgagtctgactcgctgtcg                                                                                          >  2:649529/1‑139 (MQ=14)

                                                  tgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGACTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCAGAGTCGCTGTCTGAGgctgacgcgcggtctgaatctgagtcgctgtcgg                                                                                         >  1:465509/1‑138 (MQ=14)

                                                   gaATCGCGATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctga                                                                                        <  1:117538/140‑1 (MQ=255)

                                                     aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTctgagtcgctgtctgaatctgagtcgctgtctgaat                                                                                      <  1:123844/140‑1 (MQ=255)

                                                     aTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTctgagtcgctgtctgaatctgagtcgctgtctgaat                                                                                      >  1:464963/1‑140 (MQ=255)

                                                        gCTATCTGACTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGagtcgctgtctgaatctgagtcgctgtctgaatctg                                                                                   <  1:17026/140‑1 (MQ=255)

                                                         cTATCTGCCTCTGCGTCGCCATCGCCGTCTGCGTCGCTCTCTGAGTCTGAGTCGCTGTCGGAGTCTGCGTCGCTGTCTGAATCTGAGTCGCTCTCTGACTCTGAgtcgctatctcaatctgagtcgctgtctgaatctga                                                                                  <  2:513221/140‑1 (MQ=11)

                                                         cTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAgtcgctgtctgaatctgagtcgctgtctgaatctga                                                                                  <  1:606495/140‑1 (MQ=255)

                                                             ctgaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAAGCTGAATCGCTGTCTTAGTCTGAGTCGCTGTGtgcatctgagtca                                                                              >  2:245918/1‑139 (MQ=11)

                                                               gaatctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCt                                                                            <  1:51312/140‑1 (MQ=255)

                                                                  tctgaGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCGGTCTGAGTCTGAATCGCGATCTGAATCTGGGTTGTTGCCGCAGCCAGCGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTgtc                                                                         >  1:143653/1‑140 (MQ=14)

                                                                       ggcGCTATCTGAGTCTGAGCCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCCGAATCTGAATCGCTgtctgagt                                                                    <  2:405736/138‑1 (MQ=255)

                                                                        tCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGCGTATCCTGCGGATTCTGAGTCGGTTGCGGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtc                                                                   <  1:559380/140‑1 (MQ=17)

                                                                        tCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGACTCTGAATCGCTgtctgagtc                                                                   >  1:125446/1‑140 (MQ=255)

                                                                        tCGCTATCTGAATCCGAGTCGCTGTCCGAGTCTGAGTCGCTAGCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCGGTCTGAGTCTGAATCGCGgtctgacgc                                                                   >  2:403488/1‑137 (MQ=11)

                                                                          gcgctctcagtctaagtcgccgtctgcgtccgagtcgctcTCTGAGTCTGACGCGCTCTCTGAACCTGAGTCGCTCTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTgtctgagtctg                                                                 <  2:624840/136‑1 (MQ=14)

                                                                           cTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGTGTCTGAGTCGCTgtctgcatcaga                                                                >  2:264584/1‑133 (MQ=14)

                                                                              tcggagtctgagtcgctgtctgagtatgggtaacgttctGAGTCTGACTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatc                                                             <  1:285846/137‑1 (MQ=17)

                                                                               ctgagtctgagtcgctgtctgagtctgagtcgctgtCTGAGTCTGAATCGCTATCTGAATCTGAGTCACTATCTGAGACTGAATCGCTGTCTGAATCTGAGACACTGTCTGAATCTGCATCGATGtctgagtctgactcg                                                            >  1:160893/1‑140 (MQ=14)

                                                                                 gagtctgagtcgctgtctggctccgaatcgctctctGAGTCTGAATCGCTATCTGAATCTGAGTCGCTCTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAATCTGAATCGCTGtctgagtctgaatcgct                                                          <  2:510139/140‑1 (MQ=11)

                                                                                 gagtctgagtcgctgtctgagtctgagtcgctctctGAGGCTGAGTCGCTCTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgct                                                          <  2:143851/140‑1 (MQ=14)

                                                                                    cccgagtcgctgcccgcgtcggcgtcgctctctgagtctGGATCGCTACCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatc                                                       <  2:600977/137‑1 (MQ=17)

                                                                                       gagtcgctgtctgagtctgagtcgctgtctgagtctGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctga                                                    <  2:446029/140‑1 (MQ=255)

                                                                                       gagtcgctgtctgagtctgagtcgctgtctgagtctGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTCTCTGAATCTGAGTCGCTGTCTGAGTCCGAGTCGCTGtctgagtctggatcgctctctta                                                    >  2:667757/1‑138 (MQ=14)

                                                                                          tcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTatctg                                                                                                                             >  1:384060/1‑64 (MQ=255)

                                                                                          tcgctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTatctg                                                                                                                             <  2:384060/64‑1 (MQ=16)

                                                                                            gctgtctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctg                                               <  1:188522/140‑1 (MQ=255)

                                                                                                tctgagtctgtgtcgctgtctgcttctgaGTCGCTGTCTGCGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGGCTGAGTCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatc                                           <  2:465509/140‑1 (MQ=11)

                                                                                                tctgagtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatc                                           >  1:558419/1‑140 (MQ=255)

                                                                                                  tgagtctgagtcgctgtctgagtcggaATCGCGATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgc                                         <  1:202096/140‑1 (MQ=255)

                                                                                                  tgactctgagtcgctgtctgagtctgaGTCGCTATCGGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgc                                         <  1:219050/140‑1 (MQ=255)

                                                                                                    agtctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGtctgagtctgaatcgctatctgagtctgaatcgcta                                       <  1:602331/140‑1 (MQ=255)

                                                                                                     gtcttagtcgctctctgaggctgaCTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTctgagtctgaatcgctatctgagtctgaatcgctat                                      <  2:518646/140‑1 (MQ=14)

                                                                                                       ctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTGGCTGTCTGAGTCTGCGTCGAATTCTGAACCTTAGTCGCTGTCTGAATCTGAATCGCTGTCTgaacctgaatcgctatctgagtttgagtcgctatct                                    >  1:759874/1‑140 (MQ=14)

                                                                                                       ctgagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTgagtctgaatcgctatctgagtctgaatcgctatct                                    <  1:270889/140‑1 (MQ=255)

                                                                                                        tgagtcgctctctgagtctgaCTCGCTATCTGTGTCTGAGTCGCTGTCTGAGTCTGTGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGagtctgaatcgctatctgagtctgaatcgctatctg                                   <  2:705977/140‑1 (MQ=11)

                                                                                                         gagtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAgtctgaatcgctatctgagtctgaatcgctatctga                                  >  1:411178/1‑140 (MQ=255)

                                                                                                           gtcgctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCGGAAGCGCTGTCTGAGTCGGAATCGCTACCTGAgtcggagtcgcaatctgagcctgaatcgctgtctgaag                                >  2:111433/1‑138 (MQ=11)

                                                                                                              gctgtctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGaatcgctatctgagtctgagtcgctgtctgaggctg                             >  1:219940/1‑140 (MQ=255)

                                                                                                                tggctgagtctgaCGCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAtcgctatctgagtctgaatcgctatctgagtctgaa                           <  1:315137/137‑1 (MQ=255)

                                                                                                                  tctgagtctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAAGCgctagctgagtttgaagcgatatcttagtctgcatc                         >  2:514099/1‑140 (MQ=17)

                                                                                                                        tctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGACTCACTGTc                   >  1:588468/1‑140 (MQ=255)

                                                                                                                        tccgaATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTc                   <  1:103851/137‑1 (MQ=255)

                                                                                                                         ctgaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCt                  >  1:677778/1‑140 (MQ=255)

                                                                                                                           gaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGCATCGCTATCTGAGTCTGAATCACTGTCTGa                >  1:253771/1‑140 (MQ=255)

                                                                                                                           gaATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGATGTCTGAGTATGAATCGGTATCTGAGTCTGGATAGCTATCTGAGTCTGAATCACTGTCtta                >  1:124513/1‑138 (MQ=17)

                                                                                                                             aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGt              <  1:566461/140‑1 (MQ=255)

                                                                                                                             aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGt              >  1:520406/1‑140 (MQ=255)

                                                                                                                             aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCGGAGTGTGAATCGCTATCTGAGTCTGACTCACGGTGTGAGt              >  1:500496/1‑140 (MQ=17)

                                                                                                                                 cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGCGTCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGCGTCTGAATCGCTGTCTGCGTCTGAGTCGCTGACTGAGTCTGa          >  2:331339/1‑140 (MQ=11)

                                                                                                                                 cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGa          >  1:276929/1‑140 (MQ=255)

                                                                                                                                   atctgaatctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGt        <  1:734669/140‑1 (MQ=255)

                                                                                                                                    tctgaatctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTTGCTGTCTGAATCTGAATCGCTGTCAGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAATCACTGTCTGATGCCGAATc       >  2:381561/1‑140 (MQ=14)

                                                                                                                                    tctgaatctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTATCGGAGTCTGAATCCATGTCTCCGTCCGCGTc       >  1:576736/1‑140 (MQ=17)

                                                                                                                                      tgaatctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGGGTCTGCGTCGCTATCTTAGTCTGAGTGGCGATCTGAGTCTGAGTCGCTAACTGAATACGAgagac     >  2:249187/1‑136 (MQ=11)

                                                                                                                                       gaatctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACt    >  1:404939/1‑140 (MQ=255)

                                                                                                                                        aatctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTg   <  1:526030/140‑1 (MQ=255)

                                                                                                                                         atctgaGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTGt  <  1:403488/140‑1 (MQ=255)

TCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAATCACTGTCTGAGTCCGAGTCACTGT  >  USA300TCH1516_ALE/2774916‑2775192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`