Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 111,560 T→C 10.6% R210R (CGT→CGC)  USA300HOU_RS00520 → putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE111,5600TC10.6% 213.6 / 11.7 82R210R (CGT→CGC) USA300HOU_RS00520putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (39/34);  new base C (6/3);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTT  >  USA300TCH1516_ALE/111422‑111695
                                                                                                                                          |                                                                                                                                       
tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTAGTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTa                                                                                                                                        >  1:575464/1‑140 (MQ=255)
tAAGTTTTTTGCGCAATAGAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTa                                                                                                                                        <  2:456986/140‑1 (MQ=255)
 aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATCATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                                                                                                       >  2:139022/1‑140 (MQ=255)
 aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                                                                       >  2:517761/1‑140 (MQ=255)
  aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                                                                                                      <  1:457664/140‑1 (MQ=255)
  aGTTGTTTGCGCAAGATAGCAAGTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                                                                                                      <  2:236711/140‑1 (MQ=14)
    ttttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAGTGTAAAACAATTACGCAAAAg                                                                                                                                    >  1:44048/1‑140 (MQ=255)
      ttttGCTCAAGATAGAAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGat                                                                                                                                  <  2:112834/140‑1 (MQ=255)
       tttGCGCTATTTAGCAATTTTAAAGATTTAATGTATTATAAAGATGGAGTTATATCATATTATACAGAGGTGCTTATTTTTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAGAATAACTACGTAACAGata                                                                                                                                 >  2:182509/1‑140 (MQ=18)
       tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGata                                                                                                                                 >  2:524572/1‑140 (MQ=255)
       tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGata                                                                                                                                 >  1:720797/1‑140 (MQ=255)
         tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATg                                                                                                                               <  2:582516/140‑1 (MQ=255)
           cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGat                                                                                                                             >  1:431411/1‑139 (MQ=255)
            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACa                                                                                                                            <  1:619782/140‑1 (MQ=255)
            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACa                                                                                                                            >  1:96006/1‑140 (MQ=255)
            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACa                                                                                                                            >  1:712612/1‑140 (MQ=255)
             cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                                                                           >  1:220345/1‑140 (MQ=255)
             cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCACATTATCAATAAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                                                                           >  1:555351/1‑140 (MQ=255)
                 ataGCAATTGTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACca                                                                                                                       <  1:8026/140‑1 (MQ=255)
                    gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacg                                                                                                                    >  1:30615/1‑136 (MQ=11)
                    gCAATTTTAAAGATTTAATGAATTATAAAGATGGACATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAACGTTCTATTATCTCATCATGATGATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                                                                                                    <  2:542294/140‑2 (MQ=11)
                     cAATTTTAAAGATTTACTGACTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                                                                   <  2:454700/140‑1 (MQ=255)
                      aaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACaaa                                                                                                                  >  2:582569/1‑140 (MQ=255)
                         tttGAAGATTTAATGAATTATAAAGATGGAGATAAAGCGTGTGATGCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaa                                                                                                               <  1:320020/140‑1 (MQ=18)
                                 tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                                                                       >  1:681420/1‑140 (MQ=255)
                                     aTGAATTATAAAGATGGATATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAACGCTATGACATACCAACAAATAAAGCGCCGAAGCt                                                                                                   <  2:580010/140‑1 (MQ=255)
                                      tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                                                                  <  1:289750/140‑1 (MQ=255)
                                           tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCACCAAAGCcgttgat                                                                                             >  2:493003/1‑138 (MQ=255)
                                            ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAAAAAATAAAACGCAGAAGCtgtaggtg                                                                                            >  2:147985/1‑135 (MQ=17)
                                             taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                                                                           >  1:54527/1‑140 (MQ=255)
                                              aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGGTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaa                                                                                          <  1:738431/140‑1 (MQ=255)
                                              aaaGATGGAAATATAACATGTAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaa                                                                                          <  2:261071/140‑1 (MQ=255)
                                                aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAg                                                                                        >  2:25070/1‑140 (MQ=255)
                                                    ggAGATATACCACATTATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                                                                    <  2:281/140‑1 (MQ=255)
                                                       gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                                                                 <  2:413035/140‑1 (MQ=255)
                                                        atatatCATATAATCCAGAGGTGCCGAGTTATTCATCTCAATATCAATAAACAAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                <  1:553242/140‑1 (MQ=255)
                                                        atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                <  2:525861/140‑1 (MQ=255)
                                                        atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGATGAAAGGTACAggg                                                                                >  2:396167/1‑140 (MQ=255)
                                                        atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAAATGTTGTTGAAAGCTACAggg                                                                                >  2:431511/1‑140 (MQ=255)
                                                            atCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAtt                                                                            <  1:737483/140‑1 (MQ=255)
                                                                  taATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAg                                                                      >  1:416672/1‑140 (MQ=255)
                                                                     tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                   >  1:6042/1‑140 (MQ=255)
                                                                      ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                  >  1:127036/1‑140 (MQ=255)
                                                                      ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                  <  1:677647/140‑1 (MQ=255)
                                                                      ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                  >  1:414181/1‑140 (MQ=255)
                                                                       cAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                 >  1:210195/1‑140 (MQ=255)
                                                                       cAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                 >  1:25764/1‑140 (MQ=255)
                                                                          agGTGTCGAGTTATTCAGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatca                                                              <  1:359287/140‑1 (MQ=255)
                                                                          agGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatca                                                              <  1:339473/140‑1 (MQ=255)
                                                                           ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGATACAGGGAATTAAAAAGGTGCATCAg                                                             >  2:222298/1‑140 (MQ=255)
                                                                           ggTGCCGAGTTATTCAGCTCAATATCAATTAAATAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAg                                                             >  2:665300/1‑140 (MQ=255)
                                                                            gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATATAAAAGGTTCATCAGt                                                            >  2:213212/1‑140 (MQ=255)
                                                                               ccGAGTTATTCAGCTCAATATCAATTCACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACTAACAAATAAAGAGCCGAAGCTGATGTGGAAGGGAACAGAGAATTTAAAAGGTGAATCAGGTgg                                                         >  2:404915/1‑140 (MQ=14)
                                                                               ccGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACACATAAAGCGCCGAAGCTGTTGTTGAAAGGTATAGGGAAAAAAAAAGGTTCATCAGttag                                                         >  1:203653/1‑138 (MQ=17)
                                                                               ccGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg                                                         >  1:106367/1‑140 (MQ=255)
                                                                                cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGa                                                        >  2:697219/1‑140 (MQ=255)
                                                                                 gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat                                                       >  1:672714/1‑140 (MQ=255)
                                                                                  aGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata                                                      <  1:743827/140‑1 (MQ=255)
                                                                                        tCAGATCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaa                                                <  2:141925/140‑1 (MQ=255)
                                                                                           gCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt                                             <  1:627150/140‑1 (MQ=255)
                                                                                           gCTCAATATCAATTAACTAATGATGATAATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTAAAAGGTACAGGGAATTTAAAAGGTCCATCAGTAGGACATAAAAAAAt                                             >  1:502678/1‑140 (MQ=17)
                                                                                           gCTCAACATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAGAAAt                                             >  1:122057/1‑140 (MQ=255)
                                                                                                atatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                        <  1:185683/140‑1 (MQ=255)
                                                                                                   tCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTa                                     >  1:169121/1‑140 (MQ=255)
                                                                                                    cAATTCACCAATGATGATTATAATGTAAAACCATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAc                                    <  1:467831/140‑1 (MQ=255)
                                                                                                      aTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtt                                  >  2:524783/1‑140 (MQ=255)
                                                                                                      aTTAACTAAGTATAATTATAAGGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGATGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtt                                  <  2:462950/140‑1 (MQ=17)
                                                                                                           cTAATGATGATTATAATGTAAAAAAATTACGTAATGGTTATGACAAACCAACAAATAAAGAGCAGAAGCTGTTGTTGAAAGTTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTa                             <  2:337976/140‑1 (MQ=17)
                                                                                                              atgatgATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGTTTTTAAAAGGTCCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTagag                          >  1:172921/1‑140 (MQ=255)
                                                                                                                 atgatTATAATGTAAAACAATTACGTAACAGATATGACATACCAACTCATAGACCTCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAAt                       <  1:553204/140‑1 (MQ=17)
                                                                                                                    atTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaa                    <  1:657561/140‑1 (MQ=255)
                                                                                                                      tataATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGTGCCGAAGCAGTTGTTGAAAGGTACGGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaaaa                  >  2:382769/1‑140 (MQ=255)
                                                                                                                      tataATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaaaa                  <  1:623959/140‑1 (MQ=255)
                                                                                                                        taATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATg                <  1:705777/140‑1 (MQ=255)
                                                                                                                          aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaa              <  1:697219/140‑1 (MQ=255)
                                                                                                                            gTAAAGAAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGGCGCCGCTGGTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaaa            <  2:304407/140‑1 (MQ=18)
                                                                                                                              aaaaCAATTACGTAAAAGATATGACATACCAACAACTAGCGCGACAAATCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAata          <  1:517761/140‑1 (MQ=21)
                                                                                                                                  cAATTACGTAAAAGATATCACATATGAGGAAGGTAAGTGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAATTTTTTAGAGAAGAAAAATGAAAATAttta      >  1:525192/1‑140 (MQ=18)
                                                                                                                                  cAATTACATAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAAGTACAGAGAATTTAAAAGTTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttta      <  2:730153/140‑1 (MQ=255)
                                                                                                                                    aTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttact    >  1:204261/1‑140 (MQ=255)
                                                                                                                                    aTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttact    >  1:9388/1‑140 (MQ=255)
                                                                                                                                      tACGTAAAAGATATGACATACCAACAAATAAAGCGCAGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGGTGGATATAAAAAAATTGAATTTACGCGGCTCGAAAATAAAAAAGAAAATAtgtacgtt  >  2:228650/1‑140 (MQ=14)
                                                                                                                                          |                                                                                                                                       
TAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTT  >  USA300TCH1516_ALE/111422‑111695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑