Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,314	A→C	L120V (TTA→GTA)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,314	0	A	C	100.0%	61.7 / NA	21	L120V (TTA→GTA)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/9);  total (12/9)

AATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGG  >  USA300TCH1516_ALE/1744188‑1744446                                                                                                                                |                                                                                                                                     aaTCTTTAATTTTTTCAATACCATTTAAATATGTCAATTCAATTATAAATGCAATACCTACTA‑CGATACCGCCCAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATTTGTAATTa                                                                                                                        >  2:189418/1‑141 (MQ=255)  aTCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCCATCGTACTACTAGTCGCTAATACATCATCTGTAATTat                                                                                                                       >  1:60655/1‑140 (MQ=255)        aaTTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACg                                                                                                                 <  1:125121/141‑1 (MQ=255)        aaTTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACg                                                                                                                 <  1:95143/141‑1 (MQ=255)                             atatTTCAATTCAATTATAAATGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATc                                                                                            >  1:123760/1‑141 (MQ=255)                                tttCAATTCAATTATAAATGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCttt                                                                                         <  1:177142/141‑1 (MQ=255)                                        caattATAAATGCAATACCTACTACCGAGA‑AACCGAATTTTTCAACTAATTTTATTGCTCCATCAATCGTACCACAATTAGCTAATACATCAGCTTTAATTCACACCCGTTAACCCGCTTTAACTCCATCGTTGTGCAtct                                                                                 >  2:107314/1‑139 (MQ=255)                                         aattATAAATGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGt                                                                                <  1:209101/141‑1 (MQ=255)                                              taAATGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCCCCAGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGCTTTAATTGCATCTTTGTGCATTGTTaaaa                                                                           <  1:119221/141‑1 (MQ=255)                                              taAATGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTaaaa                                                                           <  1:59089/141‑1 (MQ=255)                                               aaaTGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAAc                                                                          >  1:189330/1‑141 (MQ=255)                                                  tGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTACCACACGTTGACCTCGTTTATTTGCATCTTTGTCCATTGTTCAAACAtt                                                                       >  1:22958/1‑141 (MQ=255)                                                               a‑cGATACCGCCTAATTTTTCAACTAATTTTATTGCTACTTCAATCGTACTACCAGTAGCTAATACATTATCTGTAATTAATACACGTTGACCTGGTTTAATTTCCTCTTTTTGCCTTCTTAAAACATTTATACCATATTCt                                                          >  2:124272/1‑141 (MQ=255)                                                                 cGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCCTTGATAAGACAGTTGTACCCTATTCTa                                                         >  1:29101/1‑141 (MQ=255)                                                                   aTACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAATACATTTGTACCATATTCTAgg                                                       >  2:202959/1‑141 (MQ=255)                                                                       cGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAAAATTTGTACTATATTCTAGGTCAt                                                   >  1:190431/1‑141 (MQ=255)                                                                          cTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTATGCATTGTTAAAACCTTTGTACCATATTCCAGGTCATACt                                                >  2:77303/1‑141 (MQ=255)                                                                                          ttttATTGCTGCTTCAATCGTACCACCAGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTc                                <  2:138282/141‑1 (MQ=255)                                                                                                                 caccaGTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTc         <  1:149617/141‑1 (MQ=255)                                                                                                                      gTAGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACa    >  1:67926/1‑141 (MQ=255)                                                                                                                        aGCTAATACATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAgg  <  2:199233/141‑1 (MQ=255)                                                                                                                                |                                                                                                                                     AATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTA‑CGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGG  >  USA300TCH1516_ALE/1744188‑1744446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑