Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,726,142 A→G 6.3% Y48H (TAT→CAT)  USA300HOU_RS14025 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,726,1420AG 6.3% 218.1 / 2.3 75Y48H (TAT→CAT) USA300HOU_RS14025hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (46/23);  new base G (3/2);  total (50/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GAATGCCATGTTGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGATTTCATCCAGTTTATTCGA  >  USA300TCH1516_ALE/2726004‑2726277
                                                                                                                                          |                                                                                                                                       
gAATGCCATGTTGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGatat                                                                                                                                        <  1:134425/140‑1 (MQ=255)
gAATGCCATGTTGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGatat                                                                                                                                        <  1:479403/140‑1 (MQ=255)
gAATGCCATGTTGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGatat                                                                                                                                        <  1:227906/140‑1 (MQ=255)
  aTGCCATGTTGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATaa                                                                                                                                      <  1:263110/140‑1 (MQ=255)
    gCCATGTTGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAAtt                                                                                                                                    <  2:278427/140‑1 (MQ=255)
       aTGTTGAATTTGCTGTTGCTGGTCGTATAACTCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGAt                                                                                                                                 <  1:292588/140‑1 (MQ=255)
        tGTTGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATGTAATTGATc                                                                                                                                >  1:648642/1‑140 (MQ=255)
        tGTTGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATGTAATTGATc                                                                                                                                >  1:243733/1‑140 (MQ=255)
        tGTTGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTAATATAATTGATc                                                                                                                                >  2:24358/1‑140 (MQ=255)
          ttGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGCTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGCCTAGCCCTATTGCTCCCATAACAACGCCCACCTCCATCTTGATCTAATCGATCTa                                                                                                                              >  1:592384/1‑140 (MQ=255)
             aaTTGGCTGTTGCTGCTCGTATAACGCGTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCg                                                                                                                           <  1:111728/140‑1 (MQ=255)
                      ttgctgCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTaa                                                                                                                  >  2:365083/1‑140 (MQ=255)
                      ttgctgCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTaa                                                                                                                  >  1:102449/1‑140 (MQ=255)
                      ttgctgCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTACATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATGTAATTGATATATGGAATGAttga                                                                                                                  >  1:171933/1‑138 (MQ=255)
                       tgctgcTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAg                                                                                                                 >  2:664113/1‑140 (MQ=255)
                       tgctgcTCGTAGAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGACATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAg                                                                                                                 <  2:314470/140‑1 (MQ=255)
                        gctgctCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTACATGTTGATATAACTGATCTATCGAATGATTAAga                                                                                                                >  2:51065/1‑139 (MQ=255)
                                    aCGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCa                                                                                                    >  2:198045/1‑140 (MQ=255)
                                         tCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTAt                                                                                               <  2:296143/140‑1 (MQ=255)
                                          ccGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATc                                                                                              >  1:28798/1‑140 (MQ=255)
                                          ccGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATc                                                                                              >  1:390691/1‑140 (MQ=255)
                                           cGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACCCTCACCTAAATGTTGATATAATTGATCTATCGAGTGATTAAGCGCGTCACTTTCATCCAtaa                                                                                             >  1:242561/1‑138 (MQ=255)
                                            gTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCaa                                                                                            <  2:294046/140‑1 (MQ=255)
                                            gTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCGAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCaa                                                                                            <  1:535947/140‑1 (MQ=255)
                                             ttGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAAt                                                                                           >  1:210313/1‑140 (MQ=255)
                                               gAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGc                                                                                         <  2:475727/140‑1 (MQ=255)
                                                 aCGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCtt                                                                                       >  1:128007/1‑140 (MQ=255)
                                                   gtcgtTTCGCAAAATGAAATTCATCGCGGAACTGTACCATACGACTAGCCATATTGCTACTATAATAACGCTCACCGAATTGTTGAGATAATAGATCTATCGAATGATTAAGCTCATCACTTTAATCTATGCACGCtttt                                                                                     >  2:640050/1‑139 (MQ=255)
                                                      gtTTCGCAAAATGAACTTCATCGCAGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTACACGTGGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAAcc                                                                                  <  2:572056/140‑1 (MQ=255)
                                                      gtTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAAcc                                                                                  <  1:75451/140‑1 (MQ=255)
                                                      gtTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAAcc                                                                                  <  2:194903/140‑1 (MQ=255)
                                                              aaaTGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTACCCTCTCGCtt                                                                          >  1:620451/1‑140 (MQ=255)
                                                              aaaTGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGATTTAACCTCTCGCtt                                                                          >  2:562610/1‑140 (MQ=255)
                                                                aTGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCCCCTAAATCTTGATATAATTAATCTATCGAATGCTTAAGCGCATCGCTCTCATCTATCAATGCTTTAACACCCCGCtttt                                                                        >  2:325782/1‑140 (MQ=255)
                                                                aTGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCtttt                                                                        >  1:215629/1‑140 (MQ=255)
                                                                aTGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATAATTAAGCGCATAACTTTCATCTATCAATCCTTTAACCTCTCGCtttc                                                                        >  2:395810/1‑139 (MQ=255)
                                                                    aaTTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTACCCTCTCGCTTTtcatc                                                                    >  1:624802/1‑140 (MQ=255)
                                                                    aaTTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTtcatc                                                                    >  1:456457/1‑140 (MQ=255)
                                                                     aTTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTtcatcc                                                                   >  2:296493/1‑139 (MQ=255)
                                                                     aTTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTtcatca                                                                   <  1:365083/140‑1 (MQ=255)
                                                                      ttCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTcatcat                                                                  >  2:695656/1‑140 (MQ=255)
                                                                      ttCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTcatcat                                                                  <  1:230667/140‑1 (MQ=255)
                                                                      ttCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCCCTGCCTTTTcatcat                                                                  >  1:332850/1‑140 (MQ=255)
                                                                       tCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAGATGTTGAGATAACTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCata                                                                 <  1:20586/140‑1 (MQ=255)
                                                                       tCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCata                                                                 <  2:575649/140‑1 (MQ=255)
                                                                        cATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCatat                                                                <  2:460659/140‑1 (MQ=255)
                                                                                      tCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTg                                                  >  1:404290/1‑140 (MQ=255)
                                                                                      tCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTg                                                  >  1:404249/1‑140 (MQ=255)
                                                                                      tCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTAACGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTg                                                  >  1:699223/1‑140 (MQ=255)
                                                                                      tCCATACGACTAGCCATAGTGCTACTATAATAACGCGCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTg                                                  <  2:693704/140‑1 (MQ=255)
                                                                                        cATACGACTAGACAGATTGCTACGATAATAACGCTCACCTAAATGTTGATATAATTGATCTGTCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCTTATAAGTCATCCATTTGCt                                                <  1:314438/140‑1 (MQ=255)
                                                                                               cTAGTCATATTGCTACTATAATAACTCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGttt                                         <  2:374365/140‑1 (MQ=255)
                                                                                                 aGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCg                                       <  1:464863/140‑1 (MQ=255)
                                                                                                  gCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCCTCATATAAGTAATCCATTTGCGCATGTTTGGc                                      >  1:322998/1‑140 (MQ=255)
                                                                                                  gCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGc                                      >  1:704295/1‑140 (MQ=255)
                                                                                                  gCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGc                                      >  1:144628/1‑140 (MQ=255)
                                                                                                  gCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCATTAACCTCTCGCTTTTAATCAGATCAGTAATCCCTTATCTCATGCTTctc                                      >  2:26063/1‑138 (MQ=255)
                                                                                                    cATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCCTTTGCTCTTAGTTCGCtg                                    >  2:74914/1‑140 (MQ=255)
                                                                                                        ttGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCCTGTTTCGCTGTGat                                >  2:30179/1‑140 (MQ=255)
                                                                                                          gCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATGACTTTCATCTATCAATGTTAAAACCACACACTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGatta                              >  2:499944/1‑140 (MQ=255)
                                                                                                                ataataACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGAttattttat                        <  2:390691/140‑1 (MQ=255)
                                                                                                                ataataACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGAttattttat                        <  2:28798/140‑1 (MQ=255)
                                                                                                                   ataaCGCTCACCTAAATGTTGATATAATTGATGTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGa                     >  2:604240/1‑140 (MQ=255)
                                                                                                                    tatcGCTCTTCTAAATGTTCATGTAATTTATCTATCGAATTATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGAt                    <  2:648642/137‑1 (MQ=255)
                                                                                                                    taaCGCTCACCTAAATGTTGATGTAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGAt                    <  2:243733/140‑1 (MQ=255)
                                                                                                                    taaCGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGAt                    >  1:544808/1‑140 (MQ=255)
                                                                                                                     aaCGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTTTTGAtt                   >  2:172384/1‑140 (MQ=255)
                                                                                                                     aaCGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTCTTGAtt                   >  1:608638/1‑140 (MQ=255)
                                                                                                                       cGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGATTTc                 >  1:620672/1‑140 (MQ=255)
                                                                                                                         cTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGATTTCAt               >  2:62749/1‑140 (MQ=255)
                                                                                                                          tCACCTAAATGTTGATATAATTGATCTATCGAATGACTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCCTGTTTCGCTGTGATTATTTTATTGATTTCATc              >  2:113547/1‑140 (MQ=255)
                                                                                                                           cACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGATTTCATcc             >  1:314570/1‑140 (MQ=255)
                                                                                                                           cACCTAAATGTTGATATAATTGAGCTATCGAAGGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGATTTCATcc             >  1:705079/1‑140 (MQ=255)
                                                                                                                             ccTAAATGTTGATATAATTGATCTATCGTATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGATTTCATCCAg           >  2:503797/1‑140 (MQ=255)
                                                                                                                                 aaTGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCACGCTTTTCATCCTATCAGCACTCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGATTTCATCCAGTTTa       >  1:297863/1‑140 (MQ=255)
                                                                                                                                      taatatAATTGATCTATCGAATTATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCCTACATTTGCACTTGTTTCGCTGTGATTATTTTATTGATTTCATCCAGTTTATTCGa  <  1:666115/138‑1 (MQ=255)
                                                                                                                                      tGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGCTTATTTTATTGATTTCATCCAGTTTATTCGa  >  2:172551/1‑140 (MQ=255)
                                                                                                                                      tGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGATTTCCTCCAGTTCATTCGa  >  2:583534/1‑140 (MQ=255)
                                                                                                                                      tGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGAGTATTTTATTGATTTCATCCTGTTTATTCGa  >  2:629929/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                       
GAATGCCATGTTGAATTTGCTGTTGCTGCTCGTATAACGCTTCCGTTGAACGTCGTTTCGCAAAATGAAATTCATCGCGGAACTGTTCCATACGACTAGCCATATTGCTACTATAATAACGCTCACCTAAATGTTGATATAATTGATCTATCGAATGATTAAGCGCATCACTTTCATCTATCAATGCTTTAACCTCTCGCTTTTCATCATATAAGTCATCCATTTGCTCATGTTTCGCTGTGATTATTTTATTGATTTCATCCAGTTTATTCGA  >  USA300TCH1516_ALE/2726004‑2726277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑