Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	110,651	C→T	14.0%	D170D (GAC→GAT)	USA300HOU_RS00515 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	110,651	0	C	T	14.0%	153.0 / 11.0	67	D170D (GAC→GAT)	USA300HOU_RS00515	putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (28/28);  new base T (2/8);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 9.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  USA300TCH1516_ALE/110515‑110790                                                                                                                                         |                                                                                                                                            gATATTAGGTGAAAATGGACGAGAATAAAATCATTACAAGAGAAGAAATTTAACATTAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATGTGGCGACTTTAAAAATTTGAAAAATTATAAAGACGga                                                                                                                                          <  2:575314/140‑1 (MQ=17)  atatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATAGCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                         >  2:455204/1‑140 (MQ=255)  atatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGATCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                         >  2:125495/1‑140 (MQ=255)   tatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgaga                                                                                                                                        <  2:430129/140‑1 (MQ=255)     tCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGata                                                                                                                                      >  1:633657/1‑140 (MQ=255)     tCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGata                                                                                                                                      >  1:331310/1‑140 (MQ=255)      ccGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCAACTTTAAAAAAATGAAAAATTATAAAGACGGAGagat                                                                                                                                     >  2:171995/1‑140 (MQ=255)       cGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAtt                                                                                                                                    <  1:351749/140‑1 (MQ=255)                    cGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCag                                                                                                                       <  1:382931/140‑1 (MQ=255)                    cGATAATAAAAACATACCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCag                                                                                                                       <  1:560057/140‑1 (MQ=255)                    cGAGAATAAAATCATACCAACAGAAGAAATGAGACATGAAAAAATACAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCag                                                                                                                       <  2:200410/140‑1 (MQ=17)                        aataaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGAAATTTCATATAATCCagagat                                                                                                                   >  2:557388/1‑138 (MQ=255)                          taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATGAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGACAAATTATCAAGACGGAGATATTTCATATAATCCAGAGGTGc                                                                                                                 >  1:18133/1‑140 (MQ=255)                          taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAAGAAAAAAAGAAATCGAAAACTTTAAGGTCTTGGGTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGc                                                                                                                 <  1:574892/140‑1 (MQ=255)                            aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCg                                                                                                               <  1:589426/140‑1 (MQ=255)                                  ttCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGAAGGAGATATCTAATATAATCCAAAGGTACCGaataat                                                                                                         >  2:59002/1‑135 (MQ=17)                                    ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTc                                                                                                       <  2:297916/140‑1 (MQ=255)                                             gAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCAACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGATGCCGAGTTATTCGGCTAAata                                                                                              >  2:685917/1‑140 (MQ=255)                                                aTTAGAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                           <  2:20635/140‑1 (MQ=255)                                                     aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATCGCGACAAACAGGATGTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                      <  1:697691/140‑1 (MQ=14)                                                          aaaaaaTAAAAAAAGAAATCGAAAACTTTGAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                 <  2:450327/140‑1 (MQ=255)                                                            aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatga                                                                               <  2:331310/140‑1 (MQ=255)                                                            aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatga                                                                               <  2:633657/140‑1 (MQ=255)                                                            aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACAGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatga                                                                               >  2:247526/1‑140 (MQ=255)                                                                aaaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                           <  2:123092/139‑1 (MQ=255)                                                                  aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGAttatta                                                                         >  1:480160/1‑140 (MQ=255)                                                                  aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                         >  1:603936/1‑140 (MQ=255)                                                                  aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                         >  2:166390/1‑140 (MQ=255)                                                                   aaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCTGCTAAATATCAATTAACTAATGATGATtat                                                                        <  2:216822/140‑1 (MQ=255)                                                                    aaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCATAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtata                                                                       >  2:512805/1‑140 (MQ=255)                                                                           aTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaa                                                                >  2:494772/1‑140 (MQ=255)                                                                            tCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                               >  2:551879/1‑140 (MQ=255)                                                                            tCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                               >  1:472098/1‑140 (MQ=255)                                                                                aaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                           <  1:112266/140‑1 (MQ=255)                                                                                aaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATACTTCATATAATCCAGAGGTGCCGAGCTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                           <  2:351482/140‑1 (MQ=255)                                                                                 acatttAAGTTATTTGTTCAATATGGGGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAGCAAtt                                                          <  2:229559/137‑1 (MQ=255)                                                                                    tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGCTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGACGATTATACTGTAAAACAATTACg                                                       >  2:272063/1‑140 (MQ=255)                                                                                    tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAGAACAATTACg                                                       >  2:635719/1‑140 (MQ=255)                                                                                     ttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                      <  2:367149/140‑1 (MQ=255)                                                                                       aaGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTACGTATCAATTAACTAATGATGATTATAATGTATCACAATTTCGCat                                                    >  1:360326/1‑139 (MQ=18)                                                                                                gTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatga                                           >  1:176486/1‑140 (MQ=255)                                                                                                            gACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAg                               <  1:581843/140‑1 (MQ=255)                                                                                                               tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                            <  2:326765/140‑1 (MQ=11)                                                                                                               tttAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTACATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                            <  2:47169/140‑1 (MQ=255)                                                                                                               tttAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAACATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                            <  2:673157/140‑1 (MQ=255)                                                                                                                  aaaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                         <  2:65547/140‑1 (MQ=255)                                                                                                                   aaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAGAGCt                        >  1:121044/1‑140 (MQ=255)                                                                                                                    aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                       >  2:671701/1‑140 (MQ=255)                                                                                                                     aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCACAAGATATGATATAACGACGAGTAAAGcccc                      >  2:139458/1‑140 (MQ=255)                                                                                                                      aTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCa                     <  1:618312/140‑1 (MQ=255)                                                                                                                        ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAAATATGATATACCGACGAGTAAAGCTCCaaa                   >  2:262967/1‑140 (MQ=255)                                                                                                                         tGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTACCGGTATCGCCGTCTTAATATAAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCCAAg                  >  1:194600/1‑140 (MQ=18)                                                                                                                          gatgaagtataAAGATGGAGATATATCATATAAGCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCCCCAAAGt                 <  2:305259/133‑1 (MQ=14)                                                                                                                          aatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGt                 <  2:106459/136‑1 (MQ=255)                                                                                                                          aatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGt                 <  2:556564/136‑1 (MQ=255)                                                                                                                           aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                <  1:531901/140‑1 (MQ=255)                                                                                                                            aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAAGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATCTGATATACCGACGAGTAAAGCTCCACAGTTa               >  2:702216/1‑140 (MQ=255)                                                                                                                            aaaaTTATAAAGACGGAGATATTTCATATAATACAGAGGTGCCAAGCTATTCGGCTGCATATCTATTAACTCATGATGATTATAATGTAAAACAATTACGCAACAGCTATGCTATAACGCCGATTACAGATCCAAAgctg               >  2:318181/1‑137 (MQ=17)                                                                                                                             aaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAAGAAAGCTCCAAACTTAt              >  2:144720/1‑140 (MQ=255)                                                                                                                               aTTATAGAGATGGAGATATATCATATAATCCAGAGGTGTCGAGCTAGTCGTCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTg            >  1:102559/1‑140 (MQ=17)                                                                                                                               aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTg            >  1:539115/1‑140 (MQ=255)                                                                                                                                 tgtcaaGACGGAGATATTTCATAGAATCAAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt          <  2:409377/136‑1 (MQ=255)                                                                                                                                   tagaGATGGAGATATGTCATATAATCAAGAGGTGCCGAGTGATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa        <  2:480160/137‑1 (MQ=18)                                                                                                                                   taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa        <  1:143340/140‑1 (MQ=255)                                                                                                                                   taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa        <  1:533963/140‑1 (MQ=255)                                                                                                                                   taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa        <  1:166390/140‑1 (MQ=255)                                                                                                                                    aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa       <  2:538461/140‑1 (MQ=255)                                                                                                                                         cGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAGGCTCCAAAGTTATTGGTAAAAGGtg  >  1:182051/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                            GATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  USA300TCH1516_ALE/110515‑110790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑