Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 430,394 A→G 8.4% K27E (AAA→GAA)  nfrA → NADPH‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE430,3940AG 8.4% 168.0 / 4.5 59K27E (AAA→GAA) nfrANADPH‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (24/29);  new base G (2/3);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AAAAAGTTCAATTATTAACTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAGCATACTCAATTATTGGTATCGACGATGAGA  >  USA300TCH1516_ALE/430254‑430532
                                                                                                                                            |                                                                                                                                          
aaaaaaTTCAATTATTATGTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAAGTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGa                                                                                                                                            <  2:748412/141‑1 (MQ=255)
   aaGTTCAATTATTAACTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGaaaa                                                                                                                                         >  2:215163/1‑141 (MQ=255)
   aaGTTCAATTATTAACTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGaaaa                                                                                                                                         <  1:602693/141‑1 (MQ=255)
      ttCAATTATTAACTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGc                                                                                                                                      <  1:633136/141‑1 (MQ=255)
       tCAATTATTAACTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGca                                                                                                                                     >  2:323793/1‑141 (MQ=255)
        cAATTATTAACTTAAAAGCAAAGAAGTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGcat                                                                                                                                    <  1:282632/141‑1 (MQ=255)
          attattAACTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGCAAAAGcatcc                                                                                                                                  >  1:194998/1‑140 (MQ=255)
          attattAACTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGcatca                                                                                                                                  >  1:298917/1‑141 (MQ=255)
           ttattaACTTATAAGCAAAGAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGcatcat                                                                                                                                 <  2:442412/141‑1 (MQ=255)
                  cTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGGAAAAGCATCATTCTGTTa                                                                                                                          >  1:247799/1‑141 (MQ=255)
                  cTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGGAAAAGCATCATTCTGTTa                                                                                                                          >  1:663692/1‑141 (MQ=255)
                   ttATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAg                                                                                                                         <  2:331471/141‑1 (MQ=255)
                   ttATAAGCAAATAAATGAAATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAg                                                                                                                         <  2:105753/141‑1 (MQ=255)
                     ataAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGaa                                                                                                                       <  1:450951/141‑1 (MQ=255)
                       aaGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGaaaa                                                                                                                     <  1:442714/141‑1 (MQ=255)
                        aGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAAt                                                                                                                    >  1:528180/1‑141 (MQ=255)
                          cAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGCTAGAAAAttt                                                                                                                  >  2:466475/1‑141 (MQ=255)
                           aaaTAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTa                                                                                                                 >  1:86422/1‑141 (MQ=255)
                              taatTGCTATAAAAAAGTTTGGATGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGa                                                                                                              <  2:596128/141‑1 (MQ=255)
                              taatTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAATCATCATTCTGTTAGATAAATTAAGa                                                                                                              >  1:546290/1‑141 (MQ=255)
                              taatTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGa                                                                                                              >  2:340452/1‑141 (MQ=255)
                              aaatTGCTATAAAAAAGTTTGGACGTGAACAATGGGAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGa                                                                                                              <  2:596135/140‑1 (MQ=255)
                               aatTGCTATAAAAAAGTTTGGAAGTGTACAATTTCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGaa                                                                                                             <  2:689063/141‑1 (MQ=255)
                               aatTACTAAAAAAAAGATTGGAAGTGTACAATTGCAAGAGGAAAATTAAAAAATAATAGTAAAGAAAAAAGAAGAAAATAACGTGAAAGAAAAAATATATAAAAAAGAGAAAAAGCATCATTCTGTTAGAAAATTTAAGaa                                                                                                             <  2:670129/141‑1 (MQ=255)
                                 ttGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATa                                                                                                           <  1:72038/141‑1 (MQ=255)
                                 ttGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATa                                                                                                           <  2:99959/141‑1 (MQ=255)
                                     aataAAAAAGGTTGGACGTGTACAATTGCAATATGAAGAATTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATGATTCTGTTAGAAAATTTAAGAATAAAcc                                                                                                       <  2:251752/140‑1 (MQ=255)
                                          aaaaGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTaa                                                                                                  >  1:271561/1‑141 (MQ=255)
                                            aaGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGt                                                                                                <  2:591250/141‑1 (MQ=255)
                                              gTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGa                                                                                              >  1:333341/1‑141 (MQ=255)
                                                 tGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGa                                                                                           <  2:568477/141‑1 (MQ=255)
                                                  ggACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGAc                                                                                          >  2:454392/1‑141 (MQ=255)
                                                        gtACAATTGTAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACgttgtt                                                                                    <  1:183905/141‑1 (MQ=255)
                                                          agaaTTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTaa                                                                                  <  2:31671/139‑1 (MQ=255)
                                                          aCAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTaa                                                                                  <  1:702857/141‑1 (MQ=255)
                                                          aCAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTaa                                                                                  <  1:46962/141‑1 (MQ=255)
                                                           cAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAg                                                                                 >  1:454232/1‑141 (MQ=255)
                                                                  aagatGAAGAATTTAAATTAATTGTAAAGTAACGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTgg                                                                          <  2:612593/138‑1 (MQ=255)
                                                                  aaTATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTgg                                                                          <  1:450607/141‑1 (MQ=255)
                                                                     atGAAGATTTTAAATACATTGACACGTACCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTCAAGACGTTGTTAAGAAATTGGTaa                                                                       >  2:421776/1‑140 (MQ=255)
                                                                        aaGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGCTAGAAg                                                                    >  2:635323/1‑141 (MQ=255)
                                                                           aTTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGGCGTAGGAGCAGTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTg                                                                 <  1:176672/141‑1 (MQ=255)
                                                                              ttAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGAc                                                              >  1:526407/1‑141 (MQ=255)
                                                                                aaaTTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGACGACGTTGTTAATAAATTGTTAGAAGCTGGATaa                                                            >  2:226880/1‑141 (MQ=255)
                                                                                aaaTTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACaa                                                            >  1:81722/1‑141 (MQ=255)
                                                                                       ttGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAGGAGTTTGGTGTGCGCTGGACAAAGCGCtt                                                     <  2:346374/141‑1 (MQ=255)
                                                                                            aaGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACg                                                >  1:44498/1‑141 (MQ=255)
                                                                                                  cGAGGAGTGGGTAATATGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGt                                          <  2:223369/141‑1 (MQ=255)
                                                                                                  cGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGt                                          <  2:598309/141‑1 (MQ=255)
                                                                                                  cGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGACAAGt                                          >  2:644009/1‑141 (MQ=255)
                                                                                                       ggTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCt                                     <  2:687111/140‑1 (MQ=255)
                                                                                                       agTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCt                                     >  1:439574/1‑141 (MQ=255)
                                                                                                            gTAACGTGTCAGAACATGTATATAATCTTGTGGAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAg                                <  2:247799/141‑1 (MQ=255)
                                                                                                            gTAACGTGTCAGAACATGTATATAATCTTGTGGAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAg                                <  2:663692/141‑1 (MQ=255)
                                                                                                                  tgtCAGAACATGTATATAATCTTGTGAAAGAGCATCATTCTGTTAAAAAATTCAAGAATTAATCTTTTATTCAATACTTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAGCATACt                          <  1:286102/141‑1 (MQ=255)
                                                                                                                    tCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAGCATACTCa                        >  1:456387/1‑141 (MQ=255)
                                                                                                                       gAAAATGTATATAATCTTGTGAAAAAGAATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGATATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAGCATACTCAatt                     <  2:103870/141‑1 (MQ=255)
                                                                                                                              tatataATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGACAAGTTTCCTGCAAGCATACTCAATTATTGGTa              >  1:387843/1‑141 (MQ=255)
                                                                                                                                     tCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTACGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAGCATACTCAATTAGTGGTATcgacga       >  1:333993/1‑141 (MQ=255)
                                                                                                                                         gtaaaaaaGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAGCATACTCAATTATTGGTATCGACGATgag   <  1:559531/138‑1 (MQ=255)
                                                                                                                                          tgAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAGCATACTCAATTATTGGTATCGACGATgaga  >  1:600771/1‑141 (MQ=255)
                                                                                                                                          tgAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAGCATACTCAATTATTGGTATCGACGATgaga  <  1:215163/141‑1 (MQ=255)
                                                                                                                                          tgAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAGCATACTCAATAATTGGTATCGACGATgaga  >  1:625576/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                          
AAAAAGTTCAATTATTAACTTATAAGCAAATAATTGCTATAAAAAAGTTTGGACGTGTACAATTGCAATATGAAGATTTTAAATTAATTGTAAAGTATCGAGGAGTGGGTAACGTGTCAGAACATGTATATAATCTTGTGAAAAAGCATCATTCTGTTAGAAAATTTAAGAATAAACCTTTAAGTGAAGACGTTGTTAAGAAATTGGTAGAAGCTGGACAAAGCGCTTCGACGTCAAGTTTCCTGCAAGCATACTCAATTATTGGTATCGACGATGAGA  >  USA300TCH1516_ALE/430254‑430532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑