| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | USA300TCH1516_ALE | 2085296 = | 24 (0.980) | 13 (0.590) | 12/252 | 0.7 | 40.9% | coding (173/201 nt) | hlb_1 | Phospholipase C |
| ? | USA300TCH1516_ALE | = 2128343 | 16 (0.730) | coding (1/825 nt) | hlb_2 | Phospholipase C | |||||
ACTACATCCGTGTATTCTCATTTGTTAGAAGAAAAATTTAATGAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTGAAAAAAGAA > USA300TCH1516_ALE/2128203‑2128483 | aCTACATCCGTGTATTCTCATTTGTTAGAAGAAAAATTTAATGAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTa > 2:136653/1‑141 (MQ=255) tGTTAGAAGAAAAATTTAATGAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAata < 1:34393/141‑1 (MQ=255) tAGAAGAAAAATTTAATGAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAATTTAGGGACCCATTAGGGACTTCAAACCCAATAAATACTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAATATaa < 2:35933/141‑1 (MQ=255) agaagaAAAATTTAATGAAGAGGACAACACAACAACTAAAATTTTAGAAAGTATGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAACTACTGTTGGCACAAGGTTTCTATCTATCCAAACTGGGGGACAtataca > 2:9817/1‑139 (MQ=255) agaagaAAAATTTAATGAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTAtgtatccaaactggagacttttaaca < 1:210890‑M2/141‑27 (MQ=255) agaagaAAAATTTAATGAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAATTTAGGAACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAACGTTTCTATGTATCCAAACTGGCGGCAATATaaa > 2:240419/1‑141 (MQ=255) tttAATGAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAGTATAAACGCGCTGAtt > 2:240710/1‑141 (MQ=255) tttAATGAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAATTTAGGGACACATTAGGGATTCCAAAACCAATAAATACTGTTGTTAAAAAGTTTCTAtgtatccaaaattaaaacttttaacataaaattact < 2:42009‑M2/141‑37 (MQ=255) tttAAGGAAGAGGACAAAAAGACAACGAGTGTTTTGGGTAGGAGATAAGGTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAATATAAACGCGCTGAtt < 1:120236/141‑1 (MQ=255) ggACAAAAAAAGAACTAAAGTTTTAGAAAGGATGTAATTGAGGGATCCACTAGGGACTCCAAACCCAATAAATACTGGTGTTACAAGGTGTCTATGTATCCAAACGGGGGGCAATATAAACGCGCTGATTTAATCGGACaa < 2:48447/141‑1 (MQ=255) aacaacTAAAATTTTAGAAAGTATGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTAtgtatccaaactggagacttttaacataaaattacttatcattcaaaaagtaaaac < 2:223414‑M2/141‑57 (MQ=255) acTAAAATTTTAGAAAGTATGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTAtgtatccaacctggagacttttaacataaaattactaatcattcaaaaagtaaaacagca < 1:126825‑M2/141‑61 (MQ=255) aaTGTTAGAAAGTATGTACTTTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAaat < 1:126561/141‑1 (MQ=255) aGAAAGTATGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAatgat > 2:9013/1‑141 (MQ=255) ttaTGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTAtgtatccaaactggagacttttaacataaaattacttatcattcaaaaagtaaaacagcataatatcaaggtttat > 2:3022‑M2/2‑65 (MQ=255) gTAATTTAGGGACACATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTAtgtatcaaaactggagacttttaacataaaattacttatcattcaaaaagtaaaacagaataatatcaaggtttataact < 2:45858‑M2/141‑81 (MQ=255) ccATTAGGGACTCCAAACCCAATAAGTACTGTTGTTACAAGGTTTCTAtgtatccaaactggagacttttaacataaaattacttatcattcaaaaagtaaaacccccctgtttcaaggtttataactttatcattatcaa > 2:129327‑M2/1‑48 (MQ=255) ccATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTAtgtatccaaactggagacttttaacataaaattacttatcattcaaaaagtaaaacagcataatatcaaggtttataactttatcattatcaa > 1:96356‑M2/1‑48 (MQ=255) aTTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAATGATGTCGTAATATTCAATGAAGCAttt < 1:83496/141‑1 (MQ=255) aTTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAATGATGTCGTAATATTCAATGAAGCAttt < 2:13033/141‑1 (MQ=255) tAGGGACTCCAAACCCAATTAATACTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGa < 1:158063/141‑1 (MQ=255) cTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTAtgtatccaaactggagacttttaacataaaattacttatcattcaaaaagtaaaacagcataatatcaaggtttataactttatcattatcaataatacctca > 1:272293‑M2/1‑38 (MQ=255) aaCCCAATAAATACTGTTGTTACAAGGTTTCTAtgtatccaaactggagacttttaacataaaattacttatcattcaaaaagtaaaacagcataatatcaaggtttataactttatcattatcaataatacctcatataa < 2:195815‑M2/141‑109 (MQ=255) aataaataCTGTTGTTACAAGGTTTCTAtgtatccaaactggagacttttaacataaaattacttatcattcaaaaagtaaaacagcataatatcaaggtttataactttatcattatcaataatacctcatataaaataa < 2:16957‑M2/141‑114 (MQ=255) aCTGTTGTTACAAGGTTTCTAtgtatccaaactggagacttttaacataaaattacttatcattcaaaaagtaaaacagcataatatcaaggtttataactttatcattatcaataatacctcatataaaataaaatttta < 2:119387‑M2/141‑121 (MQ=255) aCTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAattat < 1:239594/141‑1 (MQ=255) aGGTTTCTATGTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTGa > 1:161905/1‑141 (MQ=255) ttCTATTTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTGaaaaa < 2:256692/141‑1 (MQ=255) atgtatccaaactggagacttttaacataaaattacttatcattcaaaaagtaaaacagcataatatcaaggtttataactttatcattatcaataatacctcatataaaataaaattttagggactttttagggacttta > 1:223414‑M2/1‑1 (MQ=255) aTGTATTCAAATTGAGTGTAATATAAACGTGTTGATTTAATCTGACAATCTTCTTATATTAAAAATAATGATGTCGTAATATTTAATGAAGCATTTTATAATGGTGCATCAGACAAATTATTAAGTAATGTGAAAAAAGaa < 2:167457/141‑1 (MQ=255) | ACTACATCCGTGTATTCTCATTTGTTAGAAGAAAAATTTAATGAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAATTTAGGGACCCATTAGGGACTCCAAACCCAATAAATACTGTTGTTACAAGGTTTCTATGTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTGAAAAAAGAA > USA300TCH1516_ALE/2128203‑2128483 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |