Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,479,935	T→C	T7363T (ACA→ACG)	ebh_1 ←	Extracellular matrix‑binding protein ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,479,935	0	T	C	100.0%	53.1 / NA	20	T7363T (ACA→ACG)	ebh_1	Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/11);  total (9/11)

GCGCTTGGTTTAAGTTTGGCAATTGATCAATAGCATGTTGAACATTTTGTTTCGCCGTTGCTAAGTTTGTATTACCATTTAAATCATTTTTAGCTTGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGGTGTAACGACAACATCAGGCGT‑AGCACTAATTAATGCTTCAGCTTTCGCTACTGCTTGATTATATGCATTTTGCTTATCAGGATCTGCATTGACAAAGTTACCATCAGCTTTTGTTTGTTCTT  >  USA300TCH1516_ALE/1479802‑1480056                                                                                                                                      |                                                                                                                           gcgcTTGGTTTAAGTTTGGCAATTGATCAATAGCATGTTGAACATTTTGTTTCGCCGTTGCTAAGTTTGTATTACCATTTAAATCATTTTTAGCTTGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGGCGTAACGa                                                                                                                     <  2:277782/141‑1 (MQ=255) gcgcTTGGTTTAAGTTTGACAATAGATCAATAGCATGTTGAACATTTTGTTTCGCCGTTGCTAAGATTGTATTACCATTAAAATAATTTTTAGCTTGAGTAACAATATTTAAAACTACAGTAATTTCGATAGGCATAAcaa                                                                                                                     >  2:281308/1‑139 (MQ=255)  cgcTTGGTTTAAGTTTGGCAATTGATCAATAGCATGTTGAACATTTTGTTTCGCCGTTGCTAAGTTTGTATTACCATTTAAATCATTTTTAGCTTGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGGCGTAACGac                                                                                                                    >  1:129580/1‑141 (MQ=255)          ttAAGTTTGGCAATTGATCAATAGGATGTTGAACATTTTGTTTCGCCGTTGCTAAGTTTGTATTACCATTTAAATCATTTTTAGCTTGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGGCGTAACGACAACATCAg                                                                                                            <  2:136765/141‑1 (MQ=255)            aaGTTTGGCAATTGATCAATAGCATGTTGAACATTTTGTTTCGCCGTTGCTAAGTTTGTATTACCATTTAAATCATTTTTAGCTTGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGGCGTAACGACAACATCAGGc                                                                                                          >  2:153662/1‑141 (MQ=255)            aaGTTTGGCAATTGATCAATAGCATGTTGAACATTTTGTTTCGCCGTTGCTAAGTTTGTATTACCATTTAAATCATTTTTAGCTTGCGTAACTTTATTTAACGCTGCAGTAATTTCACTAGGCGTCACGACAACATCaatc                                                                                                          >  2:40273/1‑138 (MQ=255)                   gCAATTGATCAATAGCATGTTGAACATTTTGTTTCGCCGTTGCTAAGTTTGTATTACCATTTAAATCATTTTTAGCTTGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTACGCGTAACGATCACATCAGGCGT‑AGCAc                                                                                                  >  2:278271/1‑141 (MQ=255)                         gATCCATGGCCTGTTTAAGATTTTGTTTCGCCGTTGCTAAGTTTGTATTACCATTTAACTCATTTTTAGCTTGCGTACCTTTATTTAACGCTGCAGTAATTTCGCTAGGCGTAACGACAACATCAGGCGT‑AGCACtaatta                                                                                            <  2:30555/141‑1 (MQ=255)                             aaTGGCATGTTGCACAGTTTGTTTCGGCGTTGCTAAGGTTGTATTTCCATTTAAAACATTTTTAGCTTGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGGCGTATCGTCTACGTTATGCTT‑AGCACTAATTAATGc                                                                                        <  2:231638/141‑1 (MQ=255)                                           cATTTTGTTTCGCCGTTGCTAAGTTTGTATTACCATTTAAATCATTTTTAGCTTGCGTAACTGTATTTAACGCTGCAGTAATTTCGCTAGGCGTAACGACAACATCAGGCGT‑AGCACTAATTAATGCTTCAGCTTTCGCTa                                                                          >  1:271461/1‑141 (MQ=255)                                                                         tACCATTTAAATCATTTTTAGCTTGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGGCGTAACGACAACATCAGGCGT‑AGCACTAATTAATGCTTCAGCTTTCGCTACTGCTTGATTATATGCATTTTGCTTATCAg                                            <  1:4005/141‑1 (MQ=255)                                                                                tAAATCATTTTTAGCTTGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGGCGTAACGACAACATCAGGCGT‑AGCACTAATTAATGCTTCAGCTTTCGCTACTGCTTGATTATATGCATTTTGCTTATCAGGATCTGc                                     <  1:241308/141‑1 (MQ=255)                                                                                tAAATCATTTTTAGCTTGCGTAACTTTATGTAACGCTGCAGTAATTTCGCTAGGCGTAACGACAGCATCAGGCGT‑AGCACTAATTAATGCTTCAGCTTTCGCTACTGCTTGATTATATGCATTTTGCTTATCAGGATCTGc                                     <  2:101106/141‑1 (MQ=255)                                                                                              cTTGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGGCGTAACGACAACATCAGGCGT‑AGCACTAATTAATGCTTCAGCTCTCGCTACTGCTTGATTATATGCATTTTGCTTATCAGGATCTGCATTGACAAAGTTaa                       >  2:227064/1‑140 (MQ=255)                                                                                               ttGCGTAACTTTATTTAACGCTGCAGTAACTTCGCTAGGCGT‑TCTACGTCTTCGCCTTTGAGCACTAATTAATGCTTCAGCTTTCGCTACTGCTTGATTATATGCATTTTGCTTATCAGGATCTGCATTGACAAAGTTAcc                      <  1:278271/141‑1 (MQ=255)                                                                                                tGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGTCGTAACGACAACATCAGGACT‑ATCGCTAATTAATGCTTCAGCTTTCGCTATTGCTTGATTATATGCATTGTGCTTATTAGGATCTGCATTGCCAAAGTTACCa                     <  2:32799/141‑1 (MQ=255)                                                                                                tGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGGCGTAACGACAACATCAGGCGT‑AGCACTAATTAATGCTTCAGCTTTCGCTACTGCTTGATTATATGCATTTTGCTTATCAGGATCTGCATTGACAAAGTTAcct                     >  1:123836/1‑140 (MQ=255)                                                                                                       cTTTATTTAACGCTGCAGTAATTTCGCTAGGCGTAACGACAACATCAGGCGT‑AGCACTAATTAATGCTTCAGCTTTCGCTACTGCTTGATTATATGCATTTTGATTAGCAGGATCTGCATTGACAAAGTTACCATCAGCtt              >  2:198804/1‑141 (MQ=255)                                                                                                                   ttGCAGTAATTTCGCTAGGCGTAACGACAACATCAGTCGT‑AGCACTAATTAATGCTTCAGCTTTCGCTACTGCTTGATTATATTCTTTTTGCTTATCAGGATCTGCATTGACAAAGTTACCTTCAGCTTTTGTTTGTTCtt  <  2:11063/140‑1 (MQ=255)                                                                                                                   cTGCAGTAATTTCGCTAGGCGTAACTACATCATCAGGCGT‑AGCACTAATTAATGCTTCAGCTTTCGCTACTGCTTGATTATATGCATTTTGCTTATCAGGATCTGCATTGACAAAGTTACCATCAGCTTTTGTTTGTTCtt  <  1:47954/141‑1 (MQ=255)                                                                                                                                      |                                                                                                                           GCGCTTGGTTTAAGTTTGGCAATTGATCAATAGCATGTTGAACATTTTGTTTCGCCGTTGCTAAGTTTGTATTACCATTTAAATCATTTTTAGCTTGCGTAACTTTATTTAACGCTGCAGTAATTTCGCTAGGTGTAACGACAACATCAGGCGT‑AGCACTAATTAATGCTTCAGCTTTCGCTACTGCTTGATTATATGCATTTTGCTTATCAGGATCTGCATTGACAAAGTTACCATCAGCTTTTGTTTGTTCTT  >  USA300TCH1516_ALE/1479802‑1480056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑