Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,834,002	T→C	V133A (GTA→GCA)	osmC →	Peroxiredoxin OsmC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,834,002	0	T	C	100.0%	69.8 / NA	23	V133A (GTA→GCA)	osmC	Peroxiredoxin OsmC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/14);  total (9/14)

CGGAAAATTCAGTATGTCAAAAATTGTGCACCATCCTCAAATTCAAATTCCAAGTGATCAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGTAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGC  >  USA300TCH1516_ALE/1833868‑1834105                                                                                                                                       |                                                                                                          cGGAAAATTCAGTATGTCAAAAATTGTGCACCATCCTCAAATTCAAATTCCAAGTGATCAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAGCAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGaa‑‑a                                                                                                   >  2:207918/1‑141 (MQ=255) cGGAAAATTCAGTATGTCAAAAATTGTGCACCATCCTCAAATTCAAATTCCAAGTGATCAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGaa‑‑a                                                                                                   >  2:205281/1‑141 (MQ=255) cGGAAAATTCAGTATGTCAAAAATTGTGCACCATCCTCAAATTCAAATTCCAAGTGATCAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGaa‑‑a                                                                                                   >  2:216247/1‑141 (MQ=255) cGGAAAATTCAGTATGTCAAAAATTGTGCACCATCCTCAAATTCAAATTCCAAGTGATCAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGaa‑‑a                                                                                                   >  1:20813/1‑141 (MQ=255)          caatATGTCAAACACTGTTCAACAGTCTCAAATTCAAATGACAAGTGATCAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGa                                                                                          <  1:105884/138‑1 (MQ=255)                          gtgCACCATCCTCAAATTCAAATTCCAAGTGATCAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACCTAAGAATCTATccc                                                                          >  1:144676/1‑141 (MQ=255)                           tgCACCATCCTCAAAATCAAATTCCAAGTGATCAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCca                                                                         <  2:55102/141‑1 (MQ=255)                                cATCCTCAAGTGCAAGTTCCAAGGGATCAAATAGCACAACCAATCTCAGAAGGGTCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCAtt                                                                    <  2:127132/141‑1 (MQ=255)                                       aaattcaaattcCAAGTGATCAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCa                                                             >  2:121183/1‑141 (MQ=255)                                                 tcaaaGTGAGCAAATAGCACAATTAGAAAAGCGATTATCAAAATTGATAACAATTGCAGATAATAATTGCATGATGTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCg                                                   <  2:244873/138‑1 (MQ=255)                                                   cgaGTGATGAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTACATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGtt                                                 <  2:270975/139‑1 (MQ=255)                                                      gTGATCAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAg                                              <  1:171790/141‑1 (MQ=255)                                                            aaaTAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCCGAT‑CT‑CAACAATGATGATACGCCACCAAAACCCTATCGCTTCATTCAGGCCAAATAAAATCGTTAAGTGATaa                                        <  1:207918/141‑1 (MQ=255)                                                            aaaTAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATaa                                        <  1:216247/141‑1 (MQ=255)                                                            aaaTAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATaa                                        <  2:20813/141‑1 (MQ=255)                                                              aTAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACg                                      <  1:30104/141‑1 (MQ=255)                                                                        tAGAAAAGAGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGAc                            <  2:89985/141‑1 (MQ=255)                                                                                  aTTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGt                  >  1:228969/1‑141 (MQ=255)                                                                                    tACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTa                <  1:198321/141‑1 (MQ=255)                                                                                    tACCAAAATTGACAACAATTGCAGATAATAACTGCATGATTTAAAATGCTGCAATAA‑‑ATAATGTAGACATCACAATCTATCTCATCATTAAGACCAAATAAAATCGTTAAGTTATAACGTTTGTTCGACAACATAGcacta                >  2:181281/1‑137 (MQ=255)                                                                                     aCCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTAt               <  1:221886/141‑1 (MQ=255)                                                                                                tAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGCA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGCTCGTTTACCACTATATCCATATCACTTTTTTAg    >  2:112940/1‑141 (MQ=255)                                                                                                  aCAATTGCAGATAATAATTGCATGATTTCAAATGCTGCAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGc  <  1:3644/141‑1 (MQ=255)                                                                                                                                       |                                                                                                          CGGAAAATTCAGTATGTCAAAAATTGTGCACCATCCTCAAATTCAAATTCCAAGTGATCAAATAGCACAATTAGAAAAGCGATTACCAAAATTGATAACAATTGCAGATAATAATTGCATGATTTCAAATGCTGTAAGAA‑‑ATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGC  >  USA300TCH1516_ALE/1833868‑1834105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑