| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,224,476 | T→C | P233P (CCA→CCG) | atpA ← | ATP synthase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,224,476 | 0 | T | C | 95.8% | 73.3 / NA | 24 | P233P (CCA→CCG) | atpA | ATP synthase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (17/6); minor base G (1/0); total (18/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTCACGATAAGCTGCTGCTTGTTTAGTTAAATCATCATAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAATGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCAACATTTGCTCTTACTGTTGAATCTTTTTGACCAATAGCAACATAGATACAAATCGTACCTTG > USA300TCH1516_ALE/2224358‑2224610 | aCTCACGATAAGCTGCTGCTTGTTTAGTTAAATCATCATAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGAtg > 1:5750/1‑141 (MQ=255) aaGCTGCTGCTTGTGTAGTTAAATCATCATAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTacaa > 2:217618/1‑141 (MQ=255) gctgctgctTGTTTAGTTAAATCATCATAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTacaaca > 1:112932/1‑141 (MQ=255) tgctgctTGTTTAGTTAAATCATCATAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAAt < 1:251846/141‑1 (MQ=255) gTTTAGTTAAATCATCATAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGt < 2:41477/141‑1 (MQ=255) tAGTTAAATCATCATAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTa > 1:202988/1‑141 (MQ=255) tAAATCATCATAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGGGTAGTCTAAAgc > 1:152713/1‑141 (MQ=255) atcatcATAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTAAAACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGcc > 2:155948/1‑141 (MQ=255) atcatcATAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCAATTGTTTCACCTGAATATGGTGCAATATATAATAACGGATAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGcc > 2:187344/1‑141 (MQ=255) atcatAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGc < 1:235993/141‑1 (MQ=255) aaaCGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTc > 2:219198/1‑141 (MQ=255) cATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCaa < 1:322/141‑1 (MQ=255) ttACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCAACAttt > 1:159569/1‑141 (MQ=255) aTGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCAACATTTGCTCTTACTGtt > 1:255989/1‑141 (MQ=255) tGAATTCTTCACCCATTTTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTTCTTGTCTTAACTTTTCAACATTTGCTCTTACTGTTg < 1:164808/141‑1 (MQ=255) ttcttcACCCATTGTTACACCTGAATATGGTGCAATATATAATAAGGGAGAAGGTTCAGAAGCTGATCATGATATGGCAATAGTGTAGTCTAAAGCGCCTGCATGTCTTAACTTTTCAACATTTGCTTTTACTGTTGAATc > 1:279832/1‑141 (MQ=255) ttcttcACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCAACCTTTGCTCTTACTCTTGAATc > 2:94522/1‑141 (MQ=255) tcACCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCAACATTTGCTCTTACTGTTGAATCtttt > 1:211854/1‑141 (MQ=255) aCCCATTGTTACACCTGAATATGGTGCAATATATAATAACGGAGAAAGTTCAAAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCAACATCAACGCGAACCTTTGAATCtttttt > 2:15619/1‑140 (MQ=255) aaTATGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCAACATTTGCTCTTACTGTTGAATCTTTTTGACCAATAGCAACATAGa > 2:233136/1‑141 (MQ=255) atatGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCAACATTTGCTCTTACTGTTGAATCTTTTTGACCAATAGCAACATAGAt > 2:11386/1‑141 (MQ=255) tGGTGCAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTACCTTTTCAACATTTGCTCTTCCTGTTGAATCTTTTTGACCAATAGCAACATAGATACa > 2:59884/1‑141 (MQ=255) cAATATATAATAACGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCAACATTTGCTCTTACTGTTGAATCTTTTTGACCAATAGCAACATAGATACAAATCg > 2:80730/1‑141 (MQ=255) taataaCGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCAACATTTGCTCTTACTGTTGAATCTTTTTGACCAATAGCAACATAGATACAAATCGTACCTTg < 1:70428/141‑1 (MQ=255) | ACTCACGATAAGCTGCTGCTTGTTTAGTTAAATCATCATAAACGATTAAAACATGTTTACCGTTAAACATGAATTCTTCACCCATTGTTACACCTGAATATGGTGCAATATATAATAATGGAGAAGGTTCAGAAGCTGATGCTGCTACAACAATAGTGTAGTCTAAAGCGCCTGCTTGTCTTAACTTTTCAACATTTGCTCTTACTGTTGAATCTTTTTGACCAATAGCAACATAGATACAAATCGTACCTTG > USA300TCH1516_ALE/2224358‑2224610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |