Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,657 T→C 6.6% D1253D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,6570TC 6.6% 727.0 / 2.6 250D1253D (GAT→GACsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (130/97);  new base C (4/16);  total (135/115)
Fisher's exact test for biased strand distribution p-value = 1.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

TTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGAC  >  USA300TCH1516_ALE/618567‑618747
                                                                                          |                                                                                          
ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagat                                                                                            <  1:3307640/91‑1 (MQ=39)
ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAGAGCGATTCGGACTCAGATAGCGACtcagat                                                                                            >  1:4343519/1‑91 (MQ=255)
  cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                                                          >  2:1284257/1‑91 (MQ=37)
  cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                                                          >  1:3957892/1‑91 (MQ=39)
  cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                                                          >  2:4277515/1‑91 (MQ=37)
  cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACtcagactc                                                                                          >  2:3703689/1‑91 (MQ=255)
  cagacAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                                                          <  2:5095956/91‑1 (MQ=255)
   agagagCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattca                                                                                         <  2:2066349/91‑1 (MQ=255)
    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcag                                                                                        <  1:4072411/91‑1 (MQ=38)
    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcag                                                                                        <  1:4525082/91‑1 (MQ=37)
      tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACccagagtcagat                                                                                      >  2:4118612/1‑91 (MQ=255)
       aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATa                                                                                     <  1:1780823/91‑1 (MQ=39)
        gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                                    >  2:2682295/1‑91 (MQ=37)
        gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAATCAGATAg                                                                                    >  1:3999792/1‑91 (MQ=37)
          gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTg                                                                                  <  1:2197885/91‑1 (MQ=255)
           aCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                                                                 <  2:1968709/91‑1 (MQ=255)
            cTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACGCAGATAGCGACTCAGACACAGAGAGCGac                                                                                >  2:5028495/1‑90 (MQ=11)
             tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                                               <  2:1576862/91‑1 (MQ=255)
              cAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTc                                                                              >  2:2862178/1‑91 (MQ=255)
                gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAg                                                                            <  1:1391865/91‑1 (MQ=255)
                gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGTGATACAg                                                                            >  2:1047918/1‑91 (MQ=255)
                  ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGAc                                                                          <  2:1408656/91‑1 (MQ=255)
                    cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                        <  1:5034693/91‑1 (MQ=255)
                    cGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                        <  1:876799/91‑1 (MQ=255)
                    cGGACAGCGATTCAGACTCAGATAGCGACGCAGATTCAGATAGCGATACAGACTCAGATAGCGACTCAGATTCAGAGAGTGAGACAGACTc                                                                        >  2:3632258/1‑91 (MQ=11)
                     ggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                                                       >  2:867948/1‑91 (MQ=255)
                     ggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                                                       <  1:3154483/91‑1 (MQ=255)
                     ggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                                                       >  1:2585647/1‑91 (MQ=255)
                      gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAg                                                                      >  1:3182238/1‑91 (MQ=255)
                      gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAg                                                                      >  1:3460705/1‑91 (MQ=255)
                       aCAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                                     >  1:1207979/1‑91 (MQ=255)
                          gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                                                  >  2:2625802/1‑91 (MQ=255)
                          gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAg                                                                  >  2:2970886/1‑91 (MQ=255)
                             aTTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGa                                                               <  1:2702792/91‑1 (MQ=255)
                             aTTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGa                                                               <  1:1478754/91‑1 (MQ=255)
                              ttCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc                                                              >  1:1098673/1‑91 (MQ=255)
                              ttCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc                                                              <  1:1063460/91‑1 (MQ=255)
                              ttCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc                                                              <  1:4646591/91‑1 (MQ=255)
                               tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACt                                                             <  1:4851206/91‑1 (MQ=255)
                               tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACt                                                             >  1:3166056/1‑91 (MQ=40)
                               tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGAcc                                                             >  2:1855972/1‑90 (MQ=255)
                                cAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc                                                            >  2:531336/1‑91 (MQ=255)
                                cAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc                                                            <  2:2505291/91‑1 (MQ=255)
                                  gACGCAGATAGCGACTCAGACTCAGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAg                                                          <  2:3968444/91‑1 (MQ=255)
                                    cTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGAt                                                        <  2:4972694/91‑1 (MQ=255)
                                      cAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTc                                                      >  1:497448/1‑91 (MQ=255)
                                      cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTc                                                      <  2:1681805/91‑1 (MQ=255)
                                       aGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAGTCAGACTCAGATAGCGACTCAGATTCa                                                     >  2:2102850/1‑91 (MQ=255)
                                        gATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAg                                                    >  2:1649270/1‑91 (MQ=255)
                                         aTAGCGACTCAGATTCAGATGGCGATTCAGACTCCCATAGCGACTCAGACTCAGATAGTGATTCACACTCAGATAGCGACTCAGATTCata                                                   <  2:4720901/91‑3 (MQ=11)
                                          tAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                  <  1:529897/91‑1 (MQ=255)
                                          tAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                  >  1:3710594/1‑91 (MQ=255)
                                              gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCg                                              <  1:3051485/91‑1 (MQ=255)
                                                 tcacattcagatAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                                           <  2:2358691/91‑1 (MQ=255)
                                                  cagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTc                                          >  2:1087737/1‑91 (MQ=255)
                                                  cagattcagatAGCGAGTCGGACTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACGCAGATAGCGACTCAGATTCAGACAGCGACTc                                          <  2:698640/91‑1 (MQ=11)
                                                   agattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCa                                         <  1:2736923/91‑1 (MQ=255)
                                                    gattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAg                                        <  1:928014/91‑1 (MQ=255)
                                                    gattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAg                                        <  2:5462982/91‑1 (MQ=255)
                                                       tcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGAtt                                     >  1:3478032/1‑91 (MQ=255)
                                                        cagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCACATTCAGACAGCGACGCAGACTc                                    >  2:3170525/1‑91 (MQ=255)
                                                          gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGCCTCAGATTCgg                                  >  2:3841925/1‑91 (MQ=255)
                                                            tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACACAGATAGCGACTCAGCTTCAGACAGCGACCCAGATTCGGAt                                >  2:5630927/1‑91 (MQ=255)
                                                             aGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGTGAGTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATa                               <  2:519911/91‑1 (MQ=255)
                                                               cGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc                             >  1:1952278/1‑91 (MQ=255)
                                                                gATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATACAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCAGATACGGCTAGCg                            >  2:2785242/1‑91 (MQ=17)
                                                                  ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGAc                          <  2:23832/91‑1 (MQ=255)
                                                                  ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGAc                          >  1:5404245/1‑91 (MQ=255)
                                                                  ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGAc                          <  2:4185439/91‑1 (MQ=255)
                                                                  ttCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGAc                          <  1:1679453/91‑1 (MQ=255)
                                                                    cagACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTc                        <  2:478618/89‑1 (MQ=255)
                                                                    cGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGAcac                        >  1:5126532/1‑89 (MQ=255)
                                                                    cGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTc                        >  1:2260800/1‑91 (MQ=255)
                                                                     ggACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCa                       <  2:4462414/91‑1 (MQ=255)
                                                                        cTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGAt                    >  1:2480562/1‑91 (MQ=255)
                                                                          cAGATAGCGACTCAGATTCAGACAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTc                  >  1:1276840/1‑91 (MQ=255)
                                                                            gacaGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGACTCAGATTCAg                <  2:4326533/88‑1 (MQ=11)
                                                                              tAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGAc              >  1:195586/1‑91 (MQ=255)
                                                                              tAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGAc              >  1:2891029/1‑91 (MQ=255)
                                                                              caGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGAc              <  2:1474254/90‑1 (MQ=255)
                                                                               aGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAc                                            <  2:1352477/60‑1 (MQ=35)
                                                                                gCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAg            >  1:2060286/1‑91 (MQ=255)
                                                                                gCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAg            >  2:2189657/1‑91 (MQ=255)
                                                                                gCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAg            <  1:1426731/91‑1 (MQ=255)
                                                                                gCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAg            >  1:3917599/1‑91 (MQ=255)
                                                                                 cGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGc           >  1:2946301/1‑91 (MQ=255)
                                                                                 cGACTCAGATTCAGATAGTGATTCAGACTCACATAGCGACTCAGATTCTGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGc           >  1:5504346/1‑91 (MQ=255)
                                                                                 cGACTCAGAGTCAGATAGCCAATCAGACTCAGCTCGCGACTCAGATTCAGACAGACACTCAGATCCGGAGAGCGACTCAGATTCAGACAGc           <  2:3902327/91‑1 (MQ=14)
                                                                                  gACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCa                                                     <  1:5588623/48‑1 (MQ=40)
                                                                                   aCTCAGATTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGATAGCGTCTCAGATTCAGACAGCGa         <  2:3369803/91‑1 (MQ=255)
                                                                                   aCTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGa         <  1:691500/91‑1 (MQ=255)
                                                                                    cTCAGATTCAGCTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGAGAGCCACTCAGATACAGACAgagag        >  2:5624779/1‑90 (MQ=14)
                                                                                    cTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGAc        >  1:296570/1‑91 (MQ=255)
                                                                                    cTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGAGAc        >  2:91175/1‑91 (MQ=255)
                                                                                      cagattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTc      >  2:3786766/1‑91 (MQ=255)
                                                                                      cagattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTc      >  2:3435426/1‑91 (MQ=255)
                                                                                        gattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAg    >  2:422828/1‑91 (MQ=255)
                                                                                         attcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGa   >  2:5269266/1‑91 (MQ=255)
                                                                                          ttcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATATCGACTCAGATTCAGACAGCGACTCAGAc  <  2:5408082/91‑1 (MQ=255)
                                                                                          ttcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGAc  >  1:4243942/1‑91 (MQ=255)
                                                                                          |                                                                                          
TTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGAC  >  USA300TCH1516_ALE/618567‑618747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: