Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 725,415 T→C 8.6% V101A (GTT→GCT)  dhaK → PTS‑dependent dihydroxyacetone kinase, dihydroxyacetone‑binding subunit DhaK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE725,4150TC 8.6% 144.5 / 4.3 60V101A (GTT→GCT) dhaKPTS‑dependent dihydroxyacetone kinase, dihydroxyacetone‑binding subunit DhaK
Reads supporting (aligned to +/- strand):  ref base T (33/20);  new base C (3/2);  total (37/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

ACATGAACCTGCGCATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGTCGTGGTGTTGCA  >  USA300TCH1516_ALE/725275‑725554
                                                                                                                                            |                                                                                                                                           
aCATGAACCTGCGCATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGt                                                                                                                                             <  1:581608/141‑1 (MQ=255)
  aTGAACCTGCGCATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTg                                                                                                                                           <  2:500526/141‑1 (MQ=255)
             cATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTTCACCGGATAAAATATTAGAAGCTGTTAAAGCAGTAGATACTGGGGATGGTGTATTACTAGTTGTAAAAAACGAt                                                                                                                                >  2:337133/1‑141 (MQ=255)
             cATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATCCTGGTGATGGTGTATTACTAGTTGTAAAGAACTAt                                                                                                                                >  1:109155/1‑141 (MQ=255)
             cATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTAt                                                                                                                                >  2:658774/1‑141 (MQ=255)
             cATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTAt                                                                                                                                >  2:206816/1‑141 (MQ=255)
                     ttttGGTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGa                                                                                                                        <  2:250924/141‑1 (MQ=255)
                              aGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGaa                                                                                                               <  1:579504/141‑1 (MQ=255)
                               gAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACACCTGATAAAGTATTGGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGCGACGTGATGAAt                                                                                                              >  1:306957/1‑141 (MQ=255)
                                    tATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACACCCGCTAAAATATTAGACGCTATTAAAGCAGTCGATACTGGTGATGGTGTCTTACTAGTGGTCAAAAACTATGCAGGTGACGCGATGAATTTCGa                                                                                                         >  2:377767/1‑141 (MQ=255)
                                                  gggTTTGTGGCGAAGGATTTACAGCACCTACACCTGATAAAATATTTGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGc                                                                                           <  2:426639/140‑1 (MQ=255)
                                                  cGGTTTGTGGCGAAGTATTTACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGACGTTTAGCTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGc                                                                                           >  1:653797/1‑141 (MQ=255)
                                                                gTATTTACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGAACAAGAGCTTGCACGAATGGag                                                                             >  2:449614/1‑140 (MQ=255)
                                                                    ttACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAGATGGAAGGta                                                                         >  1:497828/1‑141 (MQ=255)
                                                                      aCATCACCTACACCTGATAAAATAGTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGtata                                                                       <  1:515464/141‑1 (MQ=255)
                                                                         tCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGCAAGATATAAAt                                                                    >  2:320205/1‑141 (MQ=255)
                                                                             cTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTc                                                                <  1:136108/141‑1 (MQ=255)
                                                                             cTACACCGGATAAAATATTAGAAGCAATTAAAGCAGTAGATACTGGTGATGGTGTATAAAAAAAAAAAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTc                                                                <  2:176942/141‑1 (MQ=255)
                                                                                cacCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTACAAATGTTCaaa                                                             >  2:55660/1‑141 (MQ=255)
                                                                                       aaaaaTATTAGAAGCTAGTAAAGCAGTGGATACGGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTTTTAt                                                      <  1:452485/140‑1 (MQ=255)
                                                                                        aaaaTATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAGTGGCACAATAGCTTGCAGAAATTGAAGGGATAAATGTTCAAACTGTTAtt                                                     >  1:467199/1‑141 (MQ=255)
                                                                                        aaaaTATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTAtt                                                     <  1:638922/141‑1 (MQ=255)
                                                                                            tatTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGGTATGGTTc                                                 >  1:595118/1‑141 (MQ=255)
                                                                                            tatTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGAAGCATGGAACTATGAAGATGTCGTTATGAATTTCGAAATGGGGCAGGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTGATTGTTc                                                 >  1:156862/1‑141 (MQ=255)
                                                                                                 gAAGCTATTAAAGCAGTAGATACTGGTGATGGGGTATTACTAGTTGTAAAAAACTATGCAGGTGAGGTGATGAATTTCGAAATGGCACAAGAGCTAGGAGAAAGGGAAGGTATAAATGGTCAAACTGTTATTGTTACTgac                                            >  2:9399/1‑141 (MQ=255)
                                                                                                   aGCCATTAAAGAAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTgacga                                          <  1:634635/141‑1 (MQ=255)
                                                                                                     gtagtAATGCAGGAGATACTGTTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACa                                        >  2:389853/5‑141 (MQ=255)
                                                                                                     cTATTAAAGCAGTAGATACTTGTGATGGTGTATTACTAGCTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATATATGTTCAAACTGTTATTGTTCGTGACGACa                                        >  2:490106/1‑141 (MQ=255)
                                                                                                     cTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGCTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACa                                        >  2:83110/1‑141 (MQ=255)
                                                                                                      tATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGGGACGTGAGGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTCATTGTTCGTGGCGACAt                                       >  2:103813/1‑141 (MQ=255)
                                                                                                      tATGAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAAAACTTGCAGAAATGGAAGGTATAAATGTTCACACTGTTATTGTTCATGAGGACAt                                       >  2:244206/1‑141 (MQ=255)
                                                                                                         tAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGc                                    <  2:528740/141‑1 (MQ=255)
                                                                                                          aaaGCAGTAGATACTGGTGATGGTGTATTACTAGCTGTAACAAAATATGCAGGTGACGTGATGAATTTCGACATGGCACAAGAGTTTGTAGAAATGGAAGGGATAAATGATCAAACTGTTATGGTTCGTGACGACATTGCt                                   >  2:554768/1‑141 (MQ=255)
                                                                                                             gCAGTAGATGCAGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTTATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCtgtg                                <  1:254320/141‑1 (MQ=255)
                                                                                                             gCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCtgtg                                >  2:324866/1‑141 (MQ=255)
                                                                                                             gCAGTAGATACGGGGGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGCATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGAAGACGTTGCtgtg                                >  2:243093/1‑141 (MQ=255)
                                                                                                                gTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACa                             >  1:104502/1‑141 (MQ=255)
                                                                                                                  aGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACaaa                           >  2:553522/1‑141 (MQ=255)
                                                                                                                  aGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACaaa                           >  2:590931/1‑141 (MQ=255)
                                                                                                                    agaCTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACg                         <  1:663527/139‑1 (MQ=255)
                                                                                                                         ggTGATGGTGTATTACTCGTTGCTTATATCTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTa                    >  1:490213/1‑141 (MQ=255)
                                                                                                                         ggTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGCCAAACGAAGTa                    >  1:308439/1‑141 (MQ=255)
                                                                                                                         ggTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTa                    >  2:359760/1‑141 (MQ=255)
                                                                                                                          gTGATGGTGTATTACTAGCTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTAc                   <  1:83110/141‑1 (MQ=255)
                                                                                                                          gTGATGGTGTATTACTAGCTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCACAAATGGAAGGTATAAATGTTCAAACCGTTATTGTCTGTGTAGCCACTTCTGTGACAAACTTAGTAc                   <  1:490106/141‑1 (MQ=255)
                                                                                                                            gATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACGTTGCTGTGACAAACGAAGTACaa                 >  2:456277/1‑141 (MQ=255)
                                                                                                                               ggTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAAcgt              <  2:622154/141‑1 (MQ=255)
                                                                                                                               ggTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAAcgt              <  2:71680/141‑1 (MQ=255)
                                                                                                                               ggTGTATTACTAGTGGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAAcgt              <  2:312415/141‑1 (MQ=255)
                                                                                                                                 tgtATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAAcgtcg            >  1:237659/1‑141 (MQ=255)
                                                                                                                                  gtATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATAGTTCGTGACGACATTGCTGTGCCAAACAAAGTACAAcgtcgt           >  2:317779/1‑141 (MQ=255)
                                                                                                                                   tATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACTAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGTCgtg          <  1:417262/141‑1 (MQ=255)
                                                                                                                                   tATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGGGCTTGCAGAAATGGAAGGTATATATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGTCgtg          <  2:260841/141‑1 (MQ=255)
                                                                                                                                   tATTACTAGTTGGAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGTCgtg          <  1:594119/141‑1 (MQ=255)
                                                                                                                                        cTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGTCGTGGTGtt     >  2:213738/1‑141 (MQ=255)
                                                                                                                                        cTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGTCGTGGGGtt     >  2:648777/1‑141 (MQ=255)
                                                                                                                                        cTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGGAGTGGTGtt     >  2:124043/1‑141 (MQ=255)
                                                                                                                                         aaGTTGCAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGTCGTGGTGTTg    <  1:3476/140‑1 (MQ=255)
                                                                                                                                          tgTTGTAAAAAACTATGCACGGTACGTGACGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGTCGTGGTGTTGc   <  2:95921/140‑1 (MQ=255)
                                                                                                                                           gTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGTCGTGGTGTTGCa  >  2:494105/1‑141 (MQ=255)
                                                                                                                                           gTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGTCGTGGTGTTGCa  >  1:510354/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
ACATGAACCTGCGCATGCCGGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTACAACGTCGTGGTGTTGCA  >  USA300TCH1516_ALE/725275‑725554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑