| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,151,090 | A→G | 10.1% | K66K (AAA→AAG) | zapA → | Cell division protein ZapA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,151,090 | 0 | A | G | 10.1% | 107.8 / 4.3 | 40 | K66K (AAA→AAG) | zapA | Cell division protein ZapA |
| Reads supporting (aligned to +/- strand): ref base A (25/10); new base G (2/2); total (28/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
CAGAGCACATACGATATGTAGCACATTTAGTTGATGATAAAATAAAAGAATTAGGGTATAAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTTGTGTATTTTGTCATCGTTGGATTCAGACGAGGTTTTTG > USA300TCH1516_ALE/1150966‑1151230 | cAGAGCACATACGATATGTAGCACATTTAGTTGATGATAAAATAAAAGAATTAGGGTATCAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAATAATAAAACTagaagaaa > 2:445675/1‑140 (MQ=255) caTACGATATGTAGCACATTTAGTTGATGATAAAATAAAAGAATTAGGGTATAAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAGGTACTACTAGAAGAAGacaacca > 2:380413/1‑138 (MQ=255) caTACGATATGTAGCACATTTAGTTGATGATAAAATAAAAGAATTAGGGTATAAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAGGTACTACTAGAAGAAGAAAATCg > 2:444432/1‑141 (MQ=255) aatatGTAGCACATTTAGTTGATGATAAAATAAAAGAATTAGGGTATAAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGtt < 1:552659/140‑1 (MQ=255) gCACATTTAGTTGATGATAAAATAAAAGAATTAGGGTATAAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAaacaa > 2:264782/1‑141 (MQ=255) acaTTTAGTTGATGATAAAATAAAAGAATTAGGGTATAAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACGACTAGAAGAAGAAAATCGACGTTGGAAaaaaca > 2:162959/1‑141 (MQ=255) aGTTGATGATAAAATAAAAGAATTAGGGCATAAAGCAGCAGGTTTAGATAATTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAAACGACGTTTAAAACAACAACTTca > 2:149926/1‑141 (MQ=255) aaaaagaaaagAATTAGGGTATAAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTgca < 1:203261/140‑1 (MQ=255) aaaataaaaGAATTAGGGTATAAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTgcag < 1:614097/141‑1 (MQ=255) gAATTAGGGTATAAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTACGCATGAAAAAGTAATAGTAGAAGAAGAAATACGACGTTTGAAACAACAAATTCACCAATTGCAGCACGGTGAg > 2:658034/1‑141 (MQ=255) ggTATAAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATaaa > 2:332147/1‑141 (MQ=255) tataAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATg > 2:391695/1‑141 (MQ=255) tataAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATAAAAACACGCT‑CGGGGATGATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATg < 1:379919/141‑1 (MQ=255) gcagcaGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCAtt > 2:415062/1‑141 (MQ=255) gcagcaGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCAtt < 1:367631/141‑1 (MQ=255) gcagGTTTAGATACTTCAAGAACAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGAt < 1:424246/141‑1 (MQ=255) agGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGAttt > 1:436986/1‑141 (MQ=255) agGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATAGTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAGATTCACAAATTGGAGCAGCGTGACGAATAACTGGTCAttatatt > 2:514225/1‑136 (MQ=255) aTACTTCAAGAAAAGCACTACTAAC‑AACGGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATaa > 2:472903/1‑141 (MQ=255) aTACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATaa > 1:107134/1‑141 (MQ=255) aTACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATaa > 1:412471/1‑141 (MQ=255) aTACTTCAAGAAAAGCAATACTAAC‑TACTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATaa < 1:257553/141‑1 (MQ=255) aTACTGCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATaa < 1:546299/141‑1 (MQ=255) tACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTGTCATAAt > 1:598472/1‑141 (MQ=255) tACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAAt > 1:365474/1‑141 (MQ=255) cTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCa > 2:243268/1‑141 (MQ=255) tCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAGGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCAtt < 1:380413/141‑1 (MQ=255) tCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAGGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCAtt < 1:444432/141‑1 (MQ=255) aGACAAGCAATACTAAC‑TGCTGTCAATATTATGCATGAAAACGTACTAATAGAAGCAGAACATAGACGTTTGAAACACCAAATTCTCCAATTGCGACAGGGTGAGCAATAAATGGCCATTGATTTTATCATAATCAtcttt > 2:187680/1‑140 (MQ=255) aaaaGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGACGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGGCATTGATTTTATCATAATCATTTTCtt > 1:26528/1‑141 (MQ=255) aaaGCAATACTAAC‑TGCTGGGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGTTCATTGATTTTATCATAATCATTTTCttt > 2:376588/1‑141 (MQ=255) aaGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTtg > 2:555746/1‑141 (MQ=255) aGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTtgt > 1:308918/1‑141 (MQ=255) cAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTTgtgt > 1:343775/1‑141 (MQ=255) cAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTTgtgt > 2:574434/1‑141 (MQ=255) tACTAAC‑TGCTGTGAATATTGTGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTTGTGTAtt > 1:468234/1‑141 (MQ=255) atatTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTTGTGTATTTTGTCATCGTTGGAt < 1:243268/141‑1 (MQ=255) tattatGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTTGTGTATTTTGTCATCGTTGGAtt > 2:254061/1‑141 (MQ=255) atGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTTGTGTATTTTGTCATCGTTGGATTCAGa > 2:526100/1‑141 (MQ=255) tGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTTGTGTATTTTGTCATCGTTGGATTCAGAc < 1:391695/141‑1 (MQ=255) aaaGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTTGTGTATTTTGTCATCGTTGGATTCAGACGAGGtttt < 1:503388/141‑1 (MQ=255) aaGTACTACTAGAAGAAGAAAATCGACGTTTGCAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTACCATAATCATTTTCTTTGTGTATTTTGTCATCGTTGGATTCAGACGAGGttttt < 1:445675/141‑1 (MQ=255) aGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTTGTGTATTTTGTCATCGTTGGATTCGTACGCGGTTTTTg > 2:398802/1‑141 (MQ=255) | CAGAGCACATACGATATGTAGCACATTTAGTTGATGATAAAATAAAAGAATTAGGGTATAAAGCAGCAGGTTTAGATACTTCAAGAAAAGCAATACTAAC‑TGCTGTGAATATTATGCATGAAAAAGTACTACTAGAAGAAGAAAATCGACGTTTGAAACAACAAATTCACAAATTGCAGCAGCGTGAGCAATAAATGGTCATTGATTTTATCATAATCATTTTCTTTGTGTATTTTGTCATCGTTGGATTCAGACGAGGTTTTTG > USA300TCH1516_ALE/1150966‑1151230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |