BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000731	8,740	T→C	92.5%	I8V (ATT→GTT)	blaZ ←	beta‑lactamase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000731	8,740	0	T	C	92.5%	730.2 / 27.6	256	I8V (ATT→GTT)	blaZ	beta‑lactamase
Reads supporting (aligned to +/- strand): ref base T (7/14); new base C (139/94); total (147/109)
Fisher's exact test for biased strand distribution p-value = 2.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 266 total aligned reads displayed.

AAATTTTACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAATTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCTAAAT  >  CP000731/8603‑8877

aaaTTTTACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTaa                                                                                                                                        >  1:648013/1‑141 (MQ=255)

   ttttACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTaaaaa                                                                                                                                     <  1:115468/141‑1 (MQ=255)

   ttttACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTaaaaa                                                                                                                                     <  1:295456/141‑1 (MQ=255)

   ttttACTTCCGTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTaaaaa                                                                                                                                     >  2:407973/1‑141 (MQ=255)

     ttACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTTTTTTTTTTTTAACTCTTTGGCATGTGAACTGTTTGAATTTCTTGCACTTAAAACTAAAGTAATTGCATCTaaaattt                                                                                                                                   >  2:583968/1‑138 (MQ=255)

      tACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAATTAAAAatat                                                                                                                                  <  1:529164/141‑1 (MQ=255)

       aCTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGACTTATATTTTTTTTCGAAATCATTTAAATCTTGGGGGCACGACCGTCGTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATAtt                                                                                                                                 >  2:469144/1‑141 (MQ=255)

            cTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAATTAAAAATATTAACtt                                                                                                                            <  2:500173/141‑1 (MQ=255)

            cTTAACACTTTTATTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACtt                                                                                                                            <  1:631693/141‑1 (MQ=255)

               aCCACTTTTAGTATTTAAAGCATAAACACCAATATGCGCATTATATTTTTTTTCTATATCTTTTTACTCTTTGGCATGTGAACTGTTTGAATTACTTGCTCTTAAAACTAACGCAATAGCAACTAAAAATATTAACttttt                                                                                                                         >  2:25317/1‑141 (MQ=255)

               aCCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACttttt                                                                                                                         <  1:5573/141‑1 (MQ=255)

                ccACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTc                                                                                                                        <  2:241188/141‑1 (MQ=255)

                ccACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTc                                                                                                                        <  1:94499/141‑1 (MQ=255)

                  aCTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCaa                                                                                                                      <  1:230473/141‑1 (MQ=255)

                     tttAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGAAATTACAACTAAAAATATTAACTTTTTCAAAAt                                                                                                                   >  1:53589/1‑141 (MQ=255)

                      ttAGTACTTAAAGCATAACCCACAACATGATCCTAATACTCTCTTGCGAAATCATCTAACTCTTTGGCATGAGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAATTAAAAATATTAACTTTCTCAAAATa                                                                                                                  <  1:466788/141‑1 (MQ=255)

                       tAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATaa                                                                                                                 <  1:518987/141‑1 (MQ=255)

                       tAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGCCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACGACTAAAAATATTACCTTTTTCAAAATaa                                                                                                                 >  1:462031/1‑141 (MQ=255)

                          tATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCCAAATCAATTAACCCCTTTGCCAGTGAACTGTTTGAAATACATGCACTTAAAACTAAAGCAATTACAATTAAAAATATTAAATTTTTCAAAATAAAcc                                                                                                              >  1:297025/1‑141 (MQ=255)

                           aTTTAAAGCATAAACACCACTATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAAccc                                                                                                             <  1:59897/141‑1 (MQ=255)

                           aTTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAAccc                                                                                                             <  2:350084/141‑1 (MQ=255)

                             ttAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAAACCTc                                                                                                           >  2:562820/1‑141 (MQ=255)

                               aaaGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCg                                                                                                         <  1:502091/141‑1 (MQ=255)

                                 aGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGat                                                                                                       >  1:595993/1‑141 (MQ=255)

                                   cATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGatat                                                                                                     >  1:249465/1‑141 (MQ=255)

                                        acacCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAAACCCACGATATTACAg                                                                                                >  2:444091/1‑141 (MQ=255)

                                        acacCAATATGAGCATAATATTTGTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGCATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAATCCTCCGATATTACat                                                                                                >  1:322824/1‑140 (MQ=255)

                                         cacCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCCCTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATGTTACAGt                                                                                               >  2:613618/1‑141 (MQ=255)

                                         cacCAATATGAGCATTATATTATTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAATTAAAAATATTAACTTTTTCAAAATAAACTCTCAGATATTACGGt                                                                                               >  1:346657/1‑141 (MQ=255)

                                           ccAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTg                                                                                             <  1:162646/141‑1 (MQ=255)

                                            cAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACACTTGt                                                                                            >  2:533635/1‑141 (MQ=255)

                                              atatGAGCATTATATTTTTTTTTTTTTTTTTTTAACTCTTTGGCACGTAAACTTTTTGATTTTCTTGCAGTAAAAACTAAAGCAATTACTACTAAAAATATTAACTTTTTTAAAATAAACACTTCTATTTTATATTTGTaa                                                                                          >  2:271366/1‑141 (MQ=255)

                                              atatGAGCATTATATTTTTTTTCTAAATCATTTAATTCTTTGGCATGTGAACTGTGTAAATTACATGCACTTAAAACTAAAGCAGTTACAACTAAAAAAATTAAATTTTTCAAAATAAAATCTAAGATATTACAGTTCTaa                                                                                          >  2:392963/1‑141 (MQ=255)

                                                atGAGCATTATATTTTTTTTCTAAATCATTTAACTTTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCATCCGATATTACAGTTGTaagt                                                                                        <  2:225589/141‑3 (MQ=255)

                                                 tGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACCTTCACTTAAAACTAAAGCAATTACAACTCAAAATTTTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAtttt                                                                                       >  2:278024/1‑141 (MQ=255)

                                                 tGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAATTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTTTAGttt                                                                                       >  1:505028/1‑141 (MQ=255)

                                                 tGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAAttt                                                                                       <  2:574441/141‑1 (MQ=255)

                                                 tGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCACGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACATCTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAAttt                                                                                       <  2:605253/141‑1 (MQ=255)

                                                   aGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAAttttt                                                                                     >  1:81402/1‑141 (MQ=255)

                                                     cATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTtat                                                                                   >  2:623235/1‑141 (MQ=255)

                                                     cATTATATCTTTTTTTTTTTTTTTTTAACTCTTTGGCATGTGAACTGTTTTAATTACATGCACTTAAAACTAAAGCAATTACAATTAAAAATATTAACTTTTTCAAAATAGATCTTCCGATATTATAGTTGTAATTTTtat                                                                                   >  2:490940/1‑141 (MQ=255)

                                                       ttATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATaa                                                                                 <  1:181409/141‑1 (MQ=255)

                                                        tataGTTTTTTTCTAANTCATGCAACTCTTTGGCATGTGAACTGGTTGAATTACATGCACTTAAAACGAAAGCAATTACAACGAAAAATATGAACTTTTTCAAAATAGACCCTCCGATATTACAGTTGTAATTTTTATaaa                                                                                <  1:646855/141‑1 (MQ=255)

                                                         atatTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCAATATTACAGTTGTAATTTTTATAAAt                                                                               >  2:553789/1‑141 (MQ=255)

                                                          tatTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAAta                                                                              >  2:580108/1‑140 (MQ=255)

                                                           atTTTTTTTCTAAATCATTTAACTCTTTTGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCa                                                                             <  1:342365/141‑1 (MQ=255)

                                                           atTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTTTAATTTTTATAAAtta                                                                             >  2:172331/1‑139 (MQ=255)

                                                           atTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCa                                                                             <  1:56446/141‑1 (MQ=255)

                                                                ttttCTAAATCATTTAACTCTTTGGCATGTGAACTGTTCGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGATATTTTTATAAATCaataat                                                                        <  1:432258/141‑1 (MQ=255)

                                                                   tttAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCAATTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTTTAAATCAATAATAtt                                                                     >  1:575996/3‑141 (MQ=255)

                                                                    cTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATAGTACAGTTGTAATTTTTATAAATCAATAATATTa                                                                    >  2:372903/1‑141 (MQ=255)

                                                                         tCATTTAACTCTTTGGCATGTGAACTGTTTGACTTACATAAACTTAATACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAAtt                                                               <  1:513996/141‑1 (MQ=255)

                                                                         tCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATTTTAACTTTTTCAAAACAAACCCTCCGATTTTACAGTTGTAATTTTTATAAATCAATAATATTACAAtt                                                               >  2:140300/1‑141 (MQ=255)

                                                                              tAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAat                                                          <  2:11554/141‑1 (MQ=255)

                                                                              tAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAat                                                          >  1:362753/1‑141 (MQ=255)

                                                                                 ctctGTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATGTTTATAAATCAATAATATTACAATTGTattatt                                                       >  1:452672/1‑141 (MQ=255)

                                                                                     ttGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATGTTACAGTTGTAATTTTTATAAATCAATAAGATTACAATTGTAATATTGATg                                                   >  1:116816/1‑141 (MQ=255)

                                                                                     ttGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTCCAATTGTTATATTGAtt                                                   >  2:356611/1‑140 (MQ=255)

                                                                                     ttGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACCAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATg                                                   <  2:366885/141‑1 (MQ=255)

                                                                                       ggCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGAtttt                                                 >  1:40605/1‑138 (MQ=255)

                                                                                       ggCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTc                                                 >  1:364975/1‑141 (MQ=255)

                                                                                       ggCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTc                                                 >  1:149771/1‑141 (MQ=255)

                                                                                        gCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCa                                                >  1:474330/1‑141 (MQ=255)

                                                                                        gCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCa                                                >  1:474104/1‑141 (MQ=255)

                                                                                           tgtgAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGTTGTCAAtt                                             >  1:357386/1‑141 (MQ=255)

                                                                                           tgtgAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGtaattt                                             >  1:125707/1‑136 (MQ=255)

                                                                                           tgtgAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAAtt                                             <  2:208836/141‑1 (MQ=255)

                                                                                           tgtgAACTGTTTAAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAAtt                                             <  2:108124/141‑1 (MQ=255)

                                                                                            gtgAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTAAAttt                                            >  2:322965/1‑140 (MQ=255)

                                                                                             tgAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGt                                           >  1:408362/1‑141 (MQ=255)

                                                                                              gAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGtt                                          <  2:207215/141‑1 (MQ=255)

                                                                                                 cTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTttat                                       >  1:388080/1‑141 (MQ=255)

                                                                                                   gTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTttatta                                     <  2:420990/141‑1 (MQ=255)

                                                                                                       gAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATaaa                                 >  2:515978/1‑141 (MQ=255)

                                                                                                       gAATTACAAGCAATTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAGTCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATaaa                                 <  2:430389/141‑1 (MQ=255)

                                                                                                        aaTTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTGTAAat                                >  2:158472/1‑141 (MQ=255)

                                                                                                        aaTTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATaaaa                                >  2:650471/1‑140 (MQ=255)

                                                                                                        aaTTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAat                                >  1:581797/1‑141 (MQ=255)

                                                                                                          ttACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTTTAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAatat                              >  2:71836/1‑141 (MQ=255)

                                                                                                           tACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATAtt                             <  2:181393/141‑1 (MQ=255)

                                                                                                             cATTCACTTACAAATAAAGCAATTAAAACTAAAAATATTAACTTTGTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATACTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTg                           <  1:253920/141‑1 (MQ=255)

                                                                                                             cATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTg                           >  2:307116/1‑141 (MQ=255)

                                                                                                             cATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTg                           >  1:192907/1‑141 (MQ=255)

                                                                                                               tgtaCTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCCACGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGaa                         <  2:56766/138‑1 (MQ=255)

                                                                                                                gCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGaaa                        <  1:106325/141‑1 (MQ=255)

                                                                                                                gCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTAAAATTGTAATATTGATGTCAGTTGTTTCTTATAAatatacaaaa                        >  2:132659/1‑135 (MQ=255)

                                                                                                                     tAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAgg                   <  2:455112/141‑1 (MQ=255)

                                                                                                                     tAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCATCCGATATTACAGTTGTAATTTTTATAAATAAATAATATTACAATTGTAATTTTGATGTCAATTGATTATTATAAATAATTGAatgattg                   >  1:603475/1‑138 (MQ=255)

                                                                                                                     tAAAAATAAAGGAATGACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAgg                   <  2:397991/141‑1 (MQ=255)

                                                                                                                       aaaCTAAAGCAAATACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGgtg                 <  1:55592/141‑1 (MQ=255)

                                                                                                                        aaCTAAAGCAATTACAATTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAAAAATGATATCAATTGATTATTATAAATATTTGAAAGAAGatga                >  1:208580/1‑140 (MQ=255)

                                                                                                                         aCTAAAGCAATTACAACTAAAAATATTAACTTTTTCAGAATAAACCCTCCGATATTACAGTTGTAATTTGTATAAATCAATAATAGTACAATTGTAATTTTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTc               <  1:271366/141‑1 (MQ=255)

                                                                                                                         aCTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTc               <  2:462472/141‑1 (MQ=255)

                                                                                                                         aCTAAAGCAATTAAAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTc               <  2:250333/141‑1 (MQ=255)

                                                                                                                         aCTAAAGCAAATACAACTAAAAATATTAACTTTTTAAACAAAAACCCCCAGTTAATACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTc               >  1:487679/1‑141 (MQ=255)

                                                                                                                            aaaGCAATTACAATTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCaaa            >  1:102110/1‑141 (MQ=255)

                                                                                                                               gCAATTACAATTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATg         <  2:312018/141‑1 (MQ=255)

                                                                                                                               gCAATTACAACTAAAAATAATAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAGTTTGTATAAATCAATAATCGTAGAATTGTAATCGTGATGTCAATTGTTTATTATAAATATTTGAAAAAAGGTGTCAAAATg         >  2:89834/1‑141 (MQ=255)

                                                                                                                                  attacaactaAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCt      >  2:456433/1‑141 (MQ=255)

                                                                                                                                   ttacaactaAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCTa     <  2:596162/141‑1 (MQ=255)

AAATTTTACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAATTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCTAAAT  >  CP000731/8603‑8877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`