| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,624,673 | T→A | 100% | K192I (AAA→ATA) | malL ← | Oligo‑1,6‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,624,673 | 0 | T | A | 95.7% | 202.8 / 0.3 | 70 | K192I (AAA→ATA) | malL | Oligo‑1,6‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base A (38/28); total (41/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGTTTGTATACCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTTTATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGATCC > USA300TCH1516_ALE/1624536‑1624809 | aaGTTTGTATACCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTatat > 2:372727/1‑141 (MQ=255) gTTTGTATACCTGGCTGATTAATATCTACATCAAATGCTGGGGCATATGTTTTAGCCTCAGGTACAGGTAAGTCACCCGGTTCAAACGTCTTCTTAATATGCATACTTGCATGTACTCTAAATCCATCAATGCCTGTATCa > 1:430291/1‑141 (MQ=255) ttGTATACCTGGCTGATTCATATCTACACCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAACCGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCaaa < 1:529871/141‑1 (MQ=255) gTATACCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAacc < 1:509771/141‑1 (MQ=255) ataCCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAaccac > 1:204633/1‑141 (MQ=255) ataCCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGGGGTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATTCCTATATCAAaccac > 1:520473/1‑141 (MQ=255) taCCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTTTATCAAacaacc > 1:247527/1‑141 (MQ=255) gATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTtcatca < 1:372727/141‑1 (MQ=255) aTTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTGAATCCATCAATGCCTATATGATACCACCAGTTcatcat > 1:21032/1‑141 (MQ=255) aTTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTcatcat < 1:312112/141‑1 (MQ=255) ttCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCAtt > 2:499058/1‑141 (MQ=255) tCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCAttt > 2:91602/1‑141 (MQ=255) tatCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCaa < 2:611640/141‑1 (MQ=255) tCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAAt > 1:665114/1‑141 (MQ=255) tCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAAt > 1:264686/1‑141 (MQ=255) tCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGGACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAAt > 1:440217/1‑141 (MQ=255) cATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAg < 1:343081/141‑1 (MQ=255) cATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAg < 2:169154/141‑1 (MQ=255) tGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACCGccacact > 2:495673/1‑135 (MQ=255) gCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCTTCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTa > 2:2607/1‑141 (MQ=255) tGGGGCATATGTTTTACCCTCAGGTACAGGGAAGTCACCCGCTTCAAACGTATTCTTACTATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAAc > 1:454242/1‑141 (MQ=255) ggggCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACt > 1:28402/1‑141 (MQ=255) gggCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACtt < 1:444318/141‑1 (MQ=255) cATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCg < 1:273844/141‑1 (MQ=255) tatGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCACCCCTAACTTCCGGa > 2:325325/1‑141 (MQ=255) gTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATAGGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTa < 1:506164/141‑1 (MQ=255) ttttACCCTCAGGTACAGGTAAGTCACCAGCGTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTAc > 1:659586/1‑141 (MQ=255) tACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTCCTCTAAATCCATCAATGCCTATATCAAACCACAAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTAcccc < 2:641687/141‑1 (MQ=255) tACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTTTATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTAcccc > 1:581173/1‑141 (MQ=255) cTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAATCCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAAtt < 1:431912/141‑1 (MQ=255) cTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCCAtt > 1:545/1‑141 (MQ=255) cTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAAATTCCGGATTACCCCAAtt > 1:392101/1‑141 (MQ=255) cTCAGGTACAGGTAAGTCACCCGCGTCAAACGTCTTCTTAAGATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAAtt < 2:101487/141‑1 (MQ=255) caggtacaggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCa > 1:443927/1‑141 (MQ=255) ggtacaggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACACCAATTCaaa > 2:31569/1‑141 (MQ=255) acaggtaAGTCACCTGCTTCAAAGGTCTTCTTAATATGCGTAATTGCATCTACTTTAAATCCATCAATGCCTATATCAAACCACAAGTTCATCATTTCAAATACAGCATCTTTAACTTCTGGATTACTCGATTTAAAATcg > 2:295416/1‑140 (MQ=255) acaggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCa > 1:449557/1‑141 (MQ=255) acaggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATACATCAATGCCTATATCAAACCACCAGTTCATCATTTCACATACAGCATCTCTAACTTCCGGATTACTCCAATTCAAATCa > 1:402034/1‑141 (MQ=255) ggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGgt > 2:238592/1‑141 (MQ=255) ccGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTAc < 2:633188/141‑1 (MQ=255) ccGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTAc > 1:53889/1‑141 (MQ=255) ccGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTAc < 2:647564/141‑1 (MQ=255) ccGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTAc > 1:386472/1‑141 (MQ=255) ccGCGTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTAc < 2:193566/141‑1 (MQ=255) cGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACt > 1:599118/1‑141 (MQ=255) ccTTAAAACGTCTTCTTAATTTGCTTAAGTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATGCAAATCAGGTTGTTTTTTACTg < 2:101527/140‑1 (MQ=255) cTTCAAACGTCTTCATAATCTGCGTAATTGCATCTACTCTAAATCCATCATTGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGa < 1:145376/141‑1 (MQ=255) aaCGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATaaa < 1:278525/141‑1 (MQ=255) cGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATg > 1:573774/1‑141 (MQ=255) cGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATg > 2:254679/1‑141 (MQ=255) cGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATg < 1:380887/141‑1 (MQ=255) cGTCTTCTTAATATGCGTAATTGCAGCTACTCTAAATCCATCATTGCCGATATCAAACCACCAGTTCATAATTTCCAATACAGCAACTCTAACTCCCCGATTTCCCCAATTAAAATCAGGTTGTTTTTTACTGCATAAATg > 2:290969/1‑141 (MQ=255) ttcttctCAATACGCACAACTGCATCTGTTCTCACCCCCTCAATGCCCATTCCCAACCCCCCGTTCGCCAGTTCACATTCACCCTTTCTAACTGCCGGATTACCCCAATTCAAATCACGTTGTTCTGTACTGAATacttgc < 2:616781/140‑6 (MQ=255) tgagatGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAACCCACCAGTTCAGCATTTCAAATACAGCATCTCTAACTTCCGGAGTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAaataat < 2:214459/137‑1 (MQ=255) atatGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAatat > 2:364892/1‑141 (MQ=255) ggTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTc < 2:454242/140‑1 (MQ=255) cGTAATTGCACCTACTCTAACTCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTc < 2:382946/141‑1 (MQ=255) aaTTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGTTCAGt > 2:426306/1‑141 (MQ=255) aaTTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCAGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGt > 2:553701/1‑141 (MQ=255) aTTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCACTTCTAATCAGGTTCTTTTTTACTGCATAAATGGGACTAATATTGCTCAGTa > 2:293666/1‑141 (MQ=255) aTTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGtt > 1:176249/1‑140 (MQ=255) gCATCTACTCTAAATCCATCAATGCCTATATCACAACACCAGTTCATCACTTAAAATAAAGCATCTCTAACTTCCGGATTACCCCAACACAAATCAGGTGGTGTGTTACTTTAGAACTGGAAATAATATCGCTCAGGATTa > 2:554942/1‑141 (MQ=255) cATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAg > 2:1795/1‑141 (MQ=255) aTCTACTCTAAATCCCTCAATGCCTTTATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATGTTGCTCAGTATTAGc < 1:494347/141‑1 (MQ=255) cccaactCCATGAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACCGCATCTCTAACTTCCGGATTACCCCACTTCAAATACGGGGTCTGTTGACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCata < 2:640458/135‑1 (MQ=255) aTCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGt < 1:429311/141‑1 (MQ=255) tCAAGGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTTCTCAGTATTAGCATCATATTCCCATGTa < 1:429168/141‑1 (MQ=255) cgacgCCTATATCAAACCACCAGTTCTTCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATCCCTATGTAg < 1:488830/137‑1 (MQ=255) gCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCACTTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGATcc > 2:275562/1‑141 (MQ=255) gCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGATcc > 1:422943/1‑141 (MQ=255) gCCTATATCAAACCACCAGTTCATCATTTCAAATAAAGCATCTGTAAATTCCGGATTACCCCAATTCAAATCAGATTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCGCTGGTAGAGcc > 1:505022/1‑141 (MQ=255) | AAGTTTGTATACCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTTTATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGATCC > USA300TCH1516_ALE/1624536‑1624809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |