| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,963,768 | C→G | 100% | V365L (GTA→CTA) | hemY ← | Protoporphyrinogen oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,963,768 | 0 | C | G | 100.0% | 206.4 / NA | 63 | V365L (GTA→CTA) | hemY | Protoporphyrinogen oxidase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (32/31); total (32/31) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCTT > USA300TCH1516_ALE/1963641‑1963905 | aaaCTATTGACAATTGTAAATTCAGGATCACCTTTAAATGCCATCACTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGCATGATCAGCAACCACACTATCACCTGGTTTACCTAGATACGCACGAATc < 2:482285/141‑1 (MQ=255) cGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCaaa > 1:507619/1‑141 (MQ=255) ttGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGACTCAAAccc > 2:343766/1‑141 (MQ=255) tGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacct > 1:175153/1‑141 (MQ=255) gACAATTTTAACTTCAGGATCACCTTTAAATTTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACCGTATCACCTGGTTTACCTGGATACGCACGAATCAAAacctt < 2:330181/141‑1 (MQ=255) aTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTTTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct < 2:112762/141‑1 (MQ=255) aTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct < 2:112038/141‑1 (MQ=255) aTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct < 2:622135/141‑1 (MQ=255) aTTGTAAATTCAGGATCACCTTTAAATGTCAGCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct < 1:244258/141‑1 (MQ=255) aTTCACGAACACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGgtg < 2:283854/141‑1 (MQ=255) aCCTTTAAATGTCATCATTTGACTTAAATCTCTTCGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGATTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTCGTAAATgg > 2:440391/1‑141 (MQ=255) aCCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATgg < 2:265606/141‑1 (MQ=255) ccTTTAAATGTCATCATTTGAATTAAATCTCTACGTACAATCGATACTAATTCATAATCTGTATGATCATCAACCACAGTATCACCTGGGTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGc > 2:630612/1‑141 (MQ=255) ttAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAt > 2:81460/1‑141 (MQ=255) ttAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAt > 1:75350/1‑141 (MQ=255) ttAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAt > 2:582659/1‑141 (MQ=255) tcatcaTTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCg > 1:628993/1‑141 (MQ=255) tttGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTcc > 1:327632/1‑141 (MQ=255) cTTAAACCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTa < 2:266912/141‑1 (MQ=255) tAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACa < 1:74537/141‑1 (MQ=255) tAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACCCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACa < 1:197084/141‑1 (MQ=255) tAAATCTCTACGTACAATCCATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACa < 2:419526/141‑1 (MQ=255) aaTCTCTACGGACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATg < 1:647773/141‑1 (MQ=255) ctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTa < 2:70736/141‑1 (MQ=255) ctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAAACTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCACGTACATGCGGTa > 2:416774/1‑141 (MQ=255) tACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTaa > 1:75426/1‑141 (MQ=255) gTACAACCGATTCTAATTTATTATCTGCATGATCATCAACCACAGGATCACCTTGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGt < 1:300543/141‑1 (MQ=255) cttcAATCGATACTAATTCCTTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGt < 2:250750/138‑1 (MQ=255) tCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCCCCTAGATTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTAt > 1:456955/1‑141 (MQ=255) tCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTAt > 1:53920/1‑141 (MQ=255) cTAATTCCTTCTCTGTATGATCATCAACCACAGTAACACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCg < 1:504108/141‑1 (MQ=255) cATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTTTTTGTCTTATTACATGCGGTAATGTCTGTATCACTAGTTCTCg > 2:428600/1‑141 (MQ=255) tATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCaa < 2:507619/141‑1 (MQ=255) aTCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATAGCCAAGTACATGCGGTAATGTCTGTATCACTCGTtctctccat > 2:310209/1‑136 (MQ=255) tCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAtt < 2:137360/141‑1 (MQ=255) ccTGTACGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCCCTAATGTCTGTATCACTCGTTCTCGCAAtt < 2:369207/140‑1 (MQ=255) tGTATGATCATCAACCACAGGATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTAc < 1:119518/141‑1 (MQ=255) tGTATGATCAACAACCACATTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTAc < 2:158533/141‑1 (MQ=255) ggagcttcaACCACAGTATCCCCTGGTTTGCCTAGATACGCATGAATCAAAACCTTACCGTCCGTTGTAGTAAATGGCCATCTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAg < 2:483526/135‑1 (MQ=255) atcatcAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGcc > 1:655986/1‑141 (MQ=255) tcatcaACCACAGTATCACCTGGTTTACCTGGATACGCACGAATGAAAACCTTACCTTCCGGTGTAGTAACTGGCCATTTTTGCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCa < 2:434612/141‑1 (MQ=255) tcagcaACCACGGTATCACATGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCa < 2:162965/141‑1 (MQ=255) atcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGGTCTCGCAATTACGAAAccgtt > 2:165224/1‑138 (MQ=255) ccaCCACAGTATCCCCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTAc < 2:304918/139‑1 (MQ=255) caACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTAc > 2:103465/1‑141 (MQ=255) aaCCCCAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTAGATGAACAACCACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACTAAGCCAGTAcc > 1:453971/1‑141 (MQ=255) cacaGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGTTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatc > 2:272783/1‑141 (MQ=255) ccaGTGTCCCGTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTGCCGGTGTAGTAAATGGCCATTTTTGCGATGTCCAAGTGCATGCGGGAATGTCGGTATCACTCGTGCTCGCAATAACGATGCCAGTAAcataa > 2:408009/2‑139 (MQ=255) acaGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCCATTACGCAACCACTACcagct > 2:601659/1‑138 (MQ=255) aGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg < 2:374058/141‑1 (MQ=255) aGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg < 1:552213/141‑1 (MQ=255) tATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATggg > 1:120903/1‑141 (MQ=255) aTCACCTGGTTTACCTAGATAAGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGAGGTCCAAGTACATGCGGTAATGCCTGTATAACTCGTTCTCGCAATTACGAAGCCAGTACCATCAAggta > 2:5306/1‑139 (MQ=255) cACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAt > 1:482503/1‑141 (MQ=255) cACCCGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAt < 2:665981/141‑1 (MQ=255) tGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGTTGTGGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTc > 1:628981/1‑141 (MQ=255) tGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAACTGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTCCGAAGCCAGTGACATCATGGGTATATTc > 1:349474/1‑141 (MQ=255) tGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTc > 1:173589/1‑141 (MQ=255) tGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGACCGAGTCATTCGTTATCGCAATTACGGAGCCAGTACCATCATGGGTATTTTc > 1:651764/1‑141 (MQ=255) gTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCaa > 1:547342/1‑141 (MQ=255) ttcccTAGATACGCACGAATCAAAACCTTACCTTCCGTTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCGCTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATg < 2:376683/138‑1 (MQ=255) ttACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATg > 1:196998/1‑141 (MQ=255) cTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCtt < 1:440391/141‑1 (MQ=255) | AAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCTT > USA300TCH1516_ALE/1963641‑1963905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |