| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,260,448 | G→T | 6.7% | P260H (CCT→CAT) | gmuF ← | putative mannose‑6‑phosphate isomerase GmuF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,260,448 | 0 | G | T | 6.7% | 207.2 / 2.0 | 72 | P260H (CCT→CAT) | gmuF | putative mannose‑6‑phosphate isomerase GmuF |
| Reads supporting (aligned to +/- strand): ref base G (36/31); new base T (2/3); total (38/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
ATGTGAAATCACCTTCAAAGACACTATCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGCATTGTACTGA > USA300TCH1516_ALE/2260308‑2260585 | aTGTGAAATCACCTTCAAAGACACTATCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAg < 2:595124/141‑1 (MQ=255) ttgAAATCCCCTTCAAAGACACGATCCAAATCTTCAGAATTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGc < 1:643203/140‑1 (MQ=255) gtgAAATCACCTTCAAAGACACTATCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTTCATCACTGTAACTAAACAGAACTCTCTAGGc < 1:592401/141‑1 (MQ=255) tgtccggcAAAGAACCTATCCAAATCTTCAGAAGTCAAAATATAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCata < 2:495590/134‑1 (MQ=255) tCACCTTCAAAGACACTATCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAAAAGAACTCTCTAGGCTTCata > 1:287265/1‑141 (MQ=255) aGACACTATCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACg < 2:567425/141‑1 (MQ=255) gACACTATCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGt < 2:546397/141‑1 (MQ=255) cacTATCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTATGCTTCATATAATTTAACGTGc > 2:89989/1‑141 (MQ=255) cacTATCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGc > 2:177999/1‑141 (MQ=255) acTATCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGcc < 1:509532/141‑1 (MQ=255) acTATCCAAATCTTCAGAAGCCAAAATAAAGTTTGTACCCGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGcc < 1:16705/141‑1 (MQ=255) aTCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCTGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCGTCATATAATTTAACGTGCCAGa > 1:461246/1‑141 (MQ=255) aTCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGa > 1:162832/1‑141 (MQ=255) tCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGTACTCTCTATGCTTCATATAATTTAACGTGCAAGaa > 2:586543/1‑141 (MQ=255) ccAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGaaa > 2:459541/1‑141 (MQ=255) cAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAAt < 1:578220/141‑1 (MQ=255) cAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAAt < 1:192013/141‑1 (MQ=255) aaaTCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAAtt > 1:113199/1‑141 (MQ=255) aTCTTCAGAAGTCACAATAAAGTTTGTACCAGTAGTCAGTTTGAACATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACTCTGTAACTAAACAGAACTCTCTATGCTTCATATAATTTAACGTGCCAGAAATTTc < 1:595682/141‑1 (MQ=255) cTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTccc > 1:78761/1‑141 (MQ=255) tCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATAGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCAt > 2:605216/1‑141 (MQ=255) tCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCa > 1:215276/1‑141 (MQ=255) cAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTGCCAACACTGTAACTAAACAGAGCGCCCTAGGCTTCTTATAATTTAACCCGCCAGAAATTTCCCATTTAACCaa > 1:252142/1‑141 (MQ=255) aaTAAAGTTTGGACCAGTCGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACTCTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGt < 1:217227/141‑1 (MQ=255) aaGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAg < 1:604789/141‑1 (MQ=255) aGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGa > 2:528630/1‑141 (MQ=255) aGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGa > 2:512449/1‑141 (MQ=255) tttGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATATCCCATTTAACCAATGTAAAGaaa < 1:426092/141‑1 (MQ=255) gtagtCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGAt > 1:649056/1‑141 (MQ=255) gtagtCAGTTTGAAAATTTCACCAACGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGAt > 1:423255/1‑141 (MQ=255) gtagtCAATTTGAAAATTTCCCCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTACATATAATTTAACGTGCCAGAAATTTCCCATTTAGCCAATGTAAAGAAATCATTCGAt > 1:419369/1‑141 (MQ=255) ggtCAGTTTGAAAATTTCACCATCGACAATCATTCGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACTTGCCAGAAATTTCCCATGTAACCAATGTAAAGAAATCATTCGATAc < 2:10044/140‑1 (MQ=255) gTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAGCACAGTAACTAAACAGAACTCTCTAGGCTTCCTCTAATTTAACGTGCCAGAAATTTCCCATTTACCCAATGTAAAGAAATCATTCGATCCAAtgg > 2:2851/1‑140 (MQ=255) gTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATGATTCGATACAAtgt > 1:317246/1‑141 (MQ=255) aaaTTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACACTGTGTGTaa > 1:54714/1‑140 (MQ=255) aaaTTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTac > 1:233207/1‑141 (MQ=255) tttCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTATGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACt < 2:522680/141‑1 (MQ=255) tCACCATCGACTATCATTTGCCCTTCGCCTTCCAACACTGTACATAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATCTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTa < 1:193961/141‑1 (MQ=255) tCACCATCGACAATCATTTGCCCTTCGCCTTCCGACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCACTCGATACAATGTGTGTACACTTa < 2:370732/141‑1 (MQ=255) tCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTACCTAACCAGAACTCTCTAGGCTTCATATGATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTa < 1:354839/141‑1 (MQ=255) ccccATCTGCAATCATTTGCCCTGCGCCTTCCAACACTGTAACTAATCAGAACTCTCTAGGCTTCATAGAATGTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTAt < 1:298995/139‑1 (MQ=255) aCCATCGACAATCATTTGCCCTTCGCCTACCAACACGATAACTAAACGGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTCAACTTATg > 1:421831/1‑141 (MQ=255) aCCATCGACAATCATCTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATg > 1:494592/1‑141 (MQ=255) cATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGt > 1:430043/1‑141 (MQ=255) tttGCCCTTCGCCTTCCGATACTGCAACTAACCAGAACTCTCTAGGCTTCATATAATCTAACGTGCCAGAAATTTCCCATTGAACCAATGTAAGGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAAttt < 2:348976/141‑1 (MQ=255) ccTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCtt > 1:601693/1‑141 (MQ=255) cGCCTTCCCACTCTGTAACTACACAGAACTCTCTCTGCTCCGTATAATTTAACGTGCCAGAAATTTCCCATTTAATTAATGTCAAGAAATCATTCGATACAATGTGTGCACACTTATGGTTTTCAATAATTTCGCTTTCAg < 2:625187/141‑1 (MQ=255) ccTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGc < 1:528630/141‑1 (MQ=255) cTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCa < 2:494592/141‑1 (MQ=255) tgcAACACTGTAACGAAACAGAACTCTCTAGGCTTCATATAAATTAACGTGCCAGAAATTTCCCATTTAACCAATGGAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCaaa < 2:476718/139‑1 (MQ=255) tCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCaaa < 2:530277/141‑1 (MQ=255) ccTGTAACTAAACAGAACCCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTa < 1:371125/140‑1 (MQ=255) ctttaactaacCAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATATAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATTTTAg < 2:406960/138‑1 (MQ=255) cTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGACATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAg < 2:403623/141‑1 (MQ=255) gTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGt < 1:223318/141‑1 (MQ=255) aaaagtaCTCTCTAGGCTTCATATAATTTAACGTTCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTg < 2:22291/135‑1 (MQ=255) aaCAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTg < 1:73042/141‑1 (MQ=255) aCAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGc > 1:622815/1‑141 (MQ=255) cAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGCa > 1:49039/1‑141 (MQ=255) aGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGAGTGTACACTTCTGGTTTTCAATAATTTCACTGTCAGGCAAAATATTAGGTAATGGTGCAt > 2:148304/1‑141 (MQ=255) gAACTCTCTAGGCTTCATATAGTTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTGAGGCAAAATATTAGGTAATGGTGCAtt > 2:285623/1‑141 (MQ=255) gAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGCAtt > 1:597088/1‑141 (MQ=255) ctctctAGGCTTCATATAATTTAATGTGCCAGAAATTTCCCGTTTAACCGATGTAAATAAATCATTCGATACAGTGTGTGTACACTTTTGGGTTTAGATAATTTAGCTTTCAGGCAAAATATTAAGTAATGGTGCATAGtc > 2:285071/1‑140 (MQ=255) ctctctAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGCATTGTa < 2:543764/141‑1 (MQ=255) ctctctAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGCATTGTa > 1:98491/1‑141 (MQ=255) ctctctAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTACGGAAATATAAGAGGTAATGGTGCATTGTa > 2:136687/1‑141 (MQ=255) ctctctAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGGGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGCATTGTa < 2:84965/141‑1 (MQ=255) ctctAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGCATTGTACt > 2:73254/1‑141 (MQ=255) ctctAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGCATTGTACt > 2:116415/1‑141 (MQ=255) ctctAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGGGTACACTTATGGTATTCACTAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGCATTGTACt > 2:202650/1‑141 (MQ=255) ctAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAACAATTAGGTGATGATGCATTGTACtta > 2:609775/1‑139 (MQ=255) ctAGGCTTCATATAATTTAACGTGCCAGAAATTTCACATTTAACAAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTAGCTTTCAGGCAAAATATTAGGTACTGGTGCATTGTACTGa > 1:231447/1‑141 (MQ=255) ccaGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGCATTGTACTGa < 2:215276/139‑1 (MQ=255) | ATGTGAAATCACCTTCAAAGACACTATCCAAATCTTCAGAAGTCAAAATAAAGTTTGTACCAGTAGTCAGTTTGAAAATTTCACCATCGACAATCATTTGCCCTTCGCCTTCCAACACTGTAACTAAACAGAACTCTCTAGGCTTCATATAATTTAACGTGCCAGAAATTTCCCATTTAACCAATGTAAAGAAATCATTCGATACAATGTGTGTACACTTATGGTTTTCAATAATTTCGCTTTCAGGCAAAATATTAGGTAATGGTGCATTGTACTGA > USA300TCH1516_ALE/2260308‑2260585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |