Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 110,660 T→A 15.7% I173I (ATT→ATA)  USA300HOU_RS00515 → putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE110,6600TA15.7% 159.5 / 14.2 63I173I (ATT→ATA) USA300HOU_RS00515putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (25/27);  new base A (2/8);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTAAAAGGTTCAGGGAATTT  >  USA300TCH1516_ALE/110524‑110800
                                                                                                                                        |                                                                                                                                             
taaaaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCat                                                                                                                                           <  2:411542/139‑1 (MQ=255)
       ggTCGATAATAAAATCCTTCAAGGAGTAGAAGTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAAACGGAGATATTTCATATAAtgc                                                                                                                                    >  2:143927/1‑139 (MQ=17)
          cGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                                 <  2:100273/141‑1 (MQ=255)
          cGATAATAAAATCATGCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                                 >  1:494552/1‑141 (MQ=255)
            ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGTGATATTTCATATAATCCAGAgg                                                                                                                               >  1:249549/1‑141 (MQ=255)
                taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                           >  1:434445/1‑141 (MQ=255)
                aaaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAACTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                           <  1:261769/140‑1 (MQ=255)
                  aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAATAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                         <  1:471607/141‑1 (MQ=255)
                  aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                         >  1:547439/1‑141 (MQ=255)
                  aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                         <  1:266579/141‑1 (MQ=255)
                  aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTACCGa                                                                                                                         >  1:326498/1‑141 (MQ=255)
                  aaaCCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCGTATAATCCAGAGGTGCCGa                                                                                                                         <  1:353909/141‑1 (MQ=255)
                         tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCATTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTCttg                                                                                                                  >  1:29738/1‑140 (MQ=255)
                          ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCg                                                                                                                 >  2:160864/1‑141 (MQ=255)
                            aacaaAAGAAATTAAAGAGGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTGTGTTCAATCTGGAGACGTTAAAAATTTGAAAAATTATAAAGACTGAGATATTTCATATAATCCATAGGTGCCGAGTTATTCGGc                                                                                                               >  2:370967/1‑141 (MQ=17)
                            aacaaAAAAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                               <  2:585252/141‑1 (MQ=255)
                                 aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                          >  2:543355/1‑141 (MQ=255)
                                 aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                          >  2:425814/1‑141 (MQ=255)
                                 aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                          <  2:331842/141‑1 (MQ=255)
                                 aaGAAATTAAAGATGAAAAAAAAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTACAAGATGGGGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                          <  2:401760/141‑1 (MQ=255)
                                  aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGCGATATTTCACATAATCCAGAGGTGCCGAGTTATTCGGCTAAatt                                                                                                         >  1:355628/1‑140 (MQ=255)
                                  aGAAATTAAAGAATAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                                         <  2:487829/141‑1 (MQ=255)
                                    aaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatata                                                                                                       >  2:360582/1‑140 (MQ=255)
                                       ttAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAAACGGAGATATTTCATATAATCCAGAGGTGACGAGTTATTAGGCTAAATATCAAt                                                                                                    >  2:304346/1‑141 (MQ=255)
                                            gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                               <  1:509236/141‑1 (MQ=255)
                                                  aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                         <  2:237911/141‑1 (MQ=255)
                                                  aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                         <  1:140110/141‑1 (MQ=255)
                                                        aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTCGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                   >  2:108035/1‑141 (MQ=255)
                                                        aaaaaaGAAATCGAAAACTTTAAGTGCTGTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATCATtat                                                                                   <  2:577117/141‑1 (MQ=255)
                                                         aaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAATTAATGATGATtata                                                                                  >  1:549574/1‑141 (MQ=255)
                                                         aaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCACAGGTGCCGAGTTATCAGGCTACACAGCGACTGACGAGTGATGATtata                                                                                  >  1:12938/1‑141 (MQ=17)
                                                           aaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                                                <  2:114113/141‑1 (MQ=255)
                                                           aaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                                                <  1:405086/141‑1 (MQ=255)
                                                               aaaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                            <  2:502173/141‑1 (MQ=255)
                                                                     aaaaCTTTAAGTCCTTTGTTCAATGTCGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                      <  2:92939/141‑1 (MQ=255)
                                                                      aaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                                     <  2:344812/141‑1 (MQ=255)
                                                                          tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                                 <  1:238334/141‑1 (MQ=255)
                                                                          tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATACTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                                 <  2:168001/141‑1 (MQ=255)
                                                                                      gTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgat                                                     <  2:194297/141‑1 (MQ=255)
                                                                                      gTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgat                                                     <  2:194548/141‑1 (MQ=255)
                                                                                                    ttttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                       <  2:505185/140‑1 (MQ=11)
                                                                                                    cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                       >  2:229484/1‑141 (MQ=255)
                                                                                                    cTTTAAAAATTTGAAAAAAAATCAGGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAGACAATTACGCAAAAGATATGATATACCGACGAGTaa                                       >  1:322945/1‑141 (MQ=18)
                                                                                                     tttAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaaa                                      >  2:359919/1‑141 (MQ=255)
                                                                                                          aaaTTTGACAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                                 <  2:255132/141‑1 (MQ=255)
                                                                                                                gAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                           >  1:61476/1‑141 (MQ=255)
                                                                                                                gAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                           >  2:50196/1‑141 (MQ=255)
                                                                                                                  tgaaTTATAAAGATGGACATATACCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACCATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑At                        <  2:169282/139‑1 (MQ=17)
                                                                                                                     aTTATCAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGt                     <  2:568288/141‑1 (MQ=255)
                                                                                                                     aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGt                     <  1:522279/141‑1 (MQ=255)
                                                                                                                     aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGt                     <  1:415612/141‑1 (MQ=255)
                                                                                                                       tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTa                   <  2:310084/141‑1 (MQ=255)
                                                                                                                        ataAAGCTGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTATGTCAATTACGCACCTGTCACTGTATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTaa                  <  1:657503/141‑1 (MQ=14)
                                                                                                                          aaaGACGGAGCTATTTCATATAATCCAGAGGTGCGGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATCATGTACAACAATTACGCAAAAGCTATGCTATACCGACGAGGAACGCTCCACAGAT‑ATTGCTgaaa                >  2:580345/1‑141 (MQ=17)
                                                                                                                            agagGGAGATATCTCAGAGAATCCAGAGGTTCCGAATTAATCGGCAAAAAATCAATTAACTAATGATGATTATAATGTAAAACAATTACGAAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTAAAAgg              <  2:233752/141‑1 (MQ=17)
                                                                                                                            acaCGCAGATATTCCATATAAACCAGAGGTCCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTAAAAgg              <  1:210954/139‑1 (MQ=255)
                                                                                                                              atggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGT‑AACCAATACAGTTGATTGTTAAAAGGtt            <  2:644127/139‑1 (MQ=14)
                                                                                                                               tggAGATATATCATATAATCCAGAGGTGCCGGGTTATTCGGCTAAATACCAAGTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTAAAAGGTTc           <  1:51901/140‑1 (MQ=255)
                                                                                                                                 gagtTATATCATATAATCCAGAGGTGCCGAGATATACGGCTAAATATCAATTAACAAATGATGATTATAATGTAAAACAATTACGCAAAAGATACGATATACCGACGAGTAAAGCTCCAAAGTC‑ATTGTCAAAAGGTCCAg         >  2:437980/1‑141 (MQ=18)
                                                                                                                                 gagaTATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTAAAAGGTTCAg         >  1:391363/1‑141 (MQ=255)
                                                                                                                                   gtgagttCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTAAAAGGTTCAggg       <  2:326498/136‑1 (MQ=255)
                                                                                                                                   gaTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTAAAAGGTTCAggg       <  2:547439/141‑1 (MQ=255)
                                                                                                                                     tatTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTAAAAGGTTCAGGGaa     >  1:544407/1‑141 (MQ=255)
                                                                                                                                     tatTTCATATAATCCAGAGGTGCCGAGGTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTAAAAGGTTCAGGGaa     >  2:159758/1‑141 (MQ=255)
                                                                                                                                        ttCATATAATTCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTAAAAGGTTCAGGGAAttt  <  2:385390/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                             
TTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTT‑ATTGTTAAAAGGTTCAGGGAATTT  >  USA300TCH1516_ALE/110524‑110800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑