BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	536,758	C→T	P202L (CCA→CTA)	rplY →	50S ribosomal protein L25

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	536,758	0	C	T	100.0%	429.6 / NA	144	P202L (CCA→CTA)	rplY	50S ribosomal protein L25
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (68/76); total (68/76)

Only 100 of 144 total aligned reads displayed.

GCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGTTATTA  >  USA300TCH1516_ALE/536622‑536895

gCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGaa                                                                                                                                       >  2:470487/1‑141 (MQ=255)

gCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGaa                                                                                                                                       >  2:1154245/1‑141 (MQ=255)

 cTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAg                                                                                                                                      <  1:910142/141‑1 (MQ=255)

   gATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCGATGGAAGGCGAACAACAAACTGAAGAACTAGAAgtt                                                                                                                                    >  2:449412/1‑141 (MQ=255)

    aTGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAgttg                                                                                                                                   <  1:986247/141‑1 (MQ=255)

     tGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAgttgt                                                                                                                                  <  2:360421/141‑1 (MQ=255)

     tGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAgttgt                                                                                                                                  >  2:1037414/1‑141 (MQ=255)

             tAACTGGCGACTTCAAAATCGAAAACGATTCCGCTGAATCAGTTGTTACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAGCAAACTGAAGAACTAGAAGTTGTTGGCGaaa                                                                                                                          <  1:1111326/141‑1 (MQ=255)

             tAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGaaa                                                                                                                          <  1:942143/141‑1 (MQ=255)

             tAACAGGCGACTTCAAAATCGAAAGCGCTTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGTTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGaaa                                                                                                                          <  1:942050/141‑1 (MQ=255)

                cTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCa                                                                                                                       <  2:49299/141‑1 (MQ=255)

                cTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCa                                                                                                                       >  1:189000/1‑141 (MQ=255)

                 tGGCGACTTCAAAATCGAAAACGCTTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCaa                                                                                                                      <  1:622192/141‑1 (MQ=255)

                     gACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAaagaa                                                                                                                  >  1:536234/1‑141 (MQ=255)

                     gACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAaagaa                                                                                                                  >  1:56357/1‑141 (MQ=255)

                          cAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACga                                                                                                             <  1:500891/141‑1 (MQ=255)

                            aaaTCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCGAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACgaag                                                                                                           >  1:631231/1‑141 (MQ=255)

                             aaTCGAAAACGATTCAGCTGAAGCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGTACTAGAAGTTGTTGGCGAAAGCAAAGAAGACgaaga                                                                                                          <  1:826149/141‑1 (MQ=255)

                              aTCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACgaagaa                                                                                                         <  1:201698/141‑1 (MQ=255)

                                 gAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGaaaaa                                                                                                      >  2:630903/1‑141 (MQ=255)

                                  ggaagaaTTCAGCAGAATCAGGAGTAAGAGTAGAAGATCAAAAAAAAGAACCAACTGAAGAAGAAAACGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAaaaaaaaaaaaaaaaaaa                                                                                                     <  2:887918/135‑1 (MQ=255)

                                  gaaaCGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGaaaaaa                                                                                                     <  2:585619/140‑1 (MQ=255)

                                    aaCGATTCAGCTGAATCCGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACGGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGATGAACTAGAAGATGTTGGCGAAAGCAAAGAGGACGAAGAAAAAAcg                                                                                                   >  1:1038963/1‑140 (MQ=255)

                                    aaCGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACt                                                                                                   >  2:904685/1‑141 (MQ=255)

                                    aaCGATTCAGCCGAATCAATAGTAACAGTAGTTGCTACAACTGAAGAACCAACTGAAGAAGGAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACt                                                                                                   <  2:1178095/141‑1 (MQ=255)

                                       ggttCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGaa                                                                                                <  2:180933/139‑1 (MQ=255)

                                           cAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAGGAAATCGAAGCTATGGAGGGCGAACAACAAACTGAAGAACTAGAAGATGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGt                                                                                            >  1:391109/1‑141 (MQ=255)

                                           cAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGCCGAAGAAAAAACTGAagaga                                                                                            >  1:1041330/1‑140 (MQ=255)

                                           cAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGt                                                                                            <  1:496611/141‑1 (MQ=255)

                                             gCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTaa                                                                                          <  1:1120880/141‑1 (MQ=255)

                                                 aaTCAGTAGTAACAGCAGTTGCTCCAACGTAAGAACCAACGGAAGAAGAAATGGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTTGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAAtttt                                                                                      <  2:1061330/141‑1 (MQ=255)

                                                  aTCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTa                                                                                     <  2:804980/141‑1 (MQ=255)

                                                    cAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAACGCTGCGTTAGAAAAAACTGAATAGTAATTTTAAt                                                                                   <  2:1094211/141‑1 (MQ=255)

                                                        agtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGATATCGAAGCTATGGCAGGCGAACAACAAACTGAAGCACTAGAGGTTGTTTGCGAAAACAAAGAAGACGAAGAAAAAACTGAACACTAATCTTCATATGt                                                                               >  2:948363/1‑141 (MQ=255)

                                                        agtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGGAATTTTAATCTGt                                                                               >  1:752376/1‑141 (MQ=255)

                                                        agtaACAGTAGTTGCGCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGt                                                                               <  2:642926/141‑1 (MQ=255)

                                                         gtaACCGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTTGCGAAAGCAAAGAAGGCGAAGAAAAAACTGAAGAGTAATTTTAATCTGtt                                                                              <  1:530749/141‑1 (MQ=255)

                                                          taACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTa                                                                             <  2:101451/141‑1 (MQ=255)

                                                             cAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACAt                                                                          <  1:1016314/141‑1 (MQ=255)

                                                             cAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACAt                                                                          <  1:1095683/141‑1 (MQ=255)

                                                              agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACAtt                                                                         >  2:409658/1‑141 (MQ=255)

                                                              agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACAtt                                                                         >  1:1100840/1‑141 (MQ=255)

                                                               gtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGTGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTa                                                                        <  1:796464/141‑1 (MQ=255)

                                                                tagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTaa                                                                       >  2:268821/1‑141 (MQ=255)

                                                                tagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTaa                                                                       >  2:860173/1‑141 (MQ=255)

                                                                tagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTaa                                                                       >  2:500711/1‑141 (MQ=255)

                                                                  gtTGCTCCAACTGAAGAACCCACTGAAGAAGAAATCGCAGGGATGTAAGGGGAACAAAAAACTGAAGAACTAGAAGGTGTTGGCGAAGGCAAAGAAGACGGAGAAAAAGCTGGAGGGAAATATTAAACTGTTACATTAAAg                                                                     >  2:492226/1‑141 (MQ=255)

                                                                   ttGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGCACAACAAACTGAAGAACTAGCAGGTGCCGGCGAAAGAAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGt                                                                    <  1:459050/141‑1 (MQ=255)

                                                                   ttGCTCAAACTGAAGAACCAACTGAATAAGAAATCGAAGCTATAGAACGCGAAAAACAAACTGAAGAACTAGAAGCTGTTTGCGAAATCAAAGAAGACGAAGAAAAAACTGAAGATTAATTTTAATCTGTTACATTAAAGt                                                                    <  2:309226/141‑1 (MQ=255)

                                                                    tGCTCCAACGGAAGAACCAACTGAAGAAGAAAGCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGtt                                                                   <  1:1139426/141‑1 (MQ=255)

                                                                      cTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTCTGGAAGGAGTAAAACGGACTTAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGATGAATAAACTGAATAGTAATTTTAATCTGTTACATTAAAGtttt                                                                 >  1:993315/1‑141 (MQ=255)

                                                                      cTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGtttt                                                                 <  1:223872/141‑1 (MQ=255)

                                                                        ccaactgaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTta                                                               <  1:770051/141‑1 (MQ=255)

                                                                        ccaactgaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTta                                                               <  2:934993/141‑1 (MQ=255)

                                                                        ccaacggaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTta                                                               <  2:144225/141‑1 (MQ=255)

                                                                         caactgaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTtat                                                              <  2:189000/141‑1 (MQ=255)

                                                                           actgaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATAc                                                            >  2:97759/1‑141 (MQ=255)

                                                                           actgaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTAAATTAAAGTTTGtatca                                                            >  2:102674/1‑139 (MQ=255)

                                                                            ctgaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACt                                                           >  1:727873/1‑141 (MQ=255)

                                                                            ctgaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGGTTTTATACt                                                           >  1:228350/1‑141 (MQ=255)

                                                                                agaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGt                                                       >  1:203749/1‑141 (MQ=255)

                                                                                agaaccaactgaagaaAAAATCGAAGCTATGGAAGGCGTACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGGAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGt                                                       >  1:665756/1‑141 (MQ=255)

                                                                                 gaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGtt                                                      <  1:938303/141‑1 (MQ=255)

                                                                                    ccaactggagaaGAAATCGAAGCCATGGACGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAAAAAAAAAAAAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTaa                                                   <  2:1047858/141‑1 (MQ=255)

                                                                                       actgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGCACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACaa                                                >  2:71769/1‑141 (MQ=255)

                                                                                       actgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGACGTTGTTGGCGCAAGCAAAGAAGACGAAGAAAAAACTGCAGAGTAATTTTAATGCGTTACATCAAAGTTCTTATCCTTTGTTTACCaa                                                >  2:1098728/1‑141 (MQ=255)

                                                                                       actgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACaa                                                <  1:238990/141‑1 (MQ=255)

                                                                                        gtgaagaaGAAAAAGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAAAAAAAAAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAg                                               <  2:886527/140‑1 (MQ=255)

                                                                                         tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGc                                              <  2:860485/141‑1 (MQ=255)

                                                                                         tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTGTATCTGTTACATTAAAGTTTTTACACTTTGTTTAACAAGc                                              <  1:1124836/141‑1 (MQ=255)

                                                                                         tgaagaaGAAATCGAAGCAATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAAATTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGc                                              <  1:149382/141‑1 (MQ=255)

                                                                                          gaagaagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCa                                             >  2:928331/1‑141 (MQ=255)

                                                                                          gaagaagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCa                                             >  2:372253/1‑141 (MQ=255)

                                                                                            agaagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTCTACTTTGTTTAACAAGCACt                                           >  2:336035/1‑141 (MQ=255)

                                                                                            agaagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACt                                           <  2:536234/141‑1 (MQ=255)

                                                                                            agaagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACt                                           <  2:56357/141‑1 (MQ=255)

                                                                                              aagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACtgt                                         <  1:701413/141‑1 (MQ=255)

                                                                                              aagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACtgt                                         <  1:1068709/141‑1 (MQ=255)

                                                                                               agaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACtgtg                                        >  2:155100/1‑141 (MQ=255)

                                                                                                gaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGc                                       <  1:671211/141‑1 (MQ=255)

                                                                                                gaaATCGAAGCTAAGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGc                                       <  2:985493/141‑1 (MQ=255)

                                                                                                       aaGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTAtttt                                <  1:1091331/141‑1 (MQ=255)

                                                                                                       aaGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTAtttt                                >  2:1084898/1‑141 (MQ=255)

                                                                                                            aTGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAatat                           >  1:489863/1‑141 (MQ=255)

                                                                                                               gAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAg                        >  2:303809/1‑141 (MQ=255)

                                                                                                                  ggCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAGGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCAt                     >  1:338266/1‑141 (MQ=255)

                                                                                                                      aacaacAAACTGGGGAACTGGAAGTTGTTGGGGACATCAAAGAAGAAGAAGCGTCTCTGGGAGATGAATTGTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTCATATAAGCATGGTg                 >  2:251915/1‑141 (MQ=255)

                                                                                                                      aacaacAAACTGAAGAACTAGAAGTTGTTTTCGAAAGCAAAGAAGACGAAGAAAAAACGGAAGAGTAATTTTAATCTGTTACATTATAGTTTTTATACTTTGTTTAAGAAGCGCTCTGCTTATTTTATTATAAGctcgttg                 >  2:412751/1‑135 (MQ=255)

                                                                                                                      aacaacAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGtc                 >  1:996418/1‑140 (MQ=255)

                                                                                                                      aacaacAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTg                 >  2:995292/1‑141 (MQ=255)

                                                                                                                        caacaaACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCt               >  1:978295/1‑141 (MQ=255)

                                                                                                                        caacaaACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCt               >  1:717073/1‑141 (MQ=255)

                                                                                                                         aacaaACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCtt              <  1:1144182/141‑1 (MQ=255)

                                                                                                                           cagaCTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGTGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCtttt            <  2:516265/138‑1 (MQ=255)

                                                                                                                           caaACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGTGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCtttt            <  2:516145/141‑1 (MQ=255)

                                                                                                                           caaACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCtttt            >  2:67466/1‑141 (MQ=255)

                                                                                                                           caaACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCtttt            >  2:1194530/1‑141 (MQ=255)

                                                                                                                              aCTCAAAAACTAGAAGTAGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGCTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTtgt         <  2:802288/141‑1 (MQ=255)

                                                                                                                                tGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTgtgt       <  1:913104/141‑1 (MQ=255)

                                                                                                                                 gaagaaCTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGtt      <  2:1131802/141‑1 (MQ=255)

GCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGTTATTA  >  USA300TCH1516_ALE/536622‑536895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`