Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,298,108	T→A	intergenic (+4/‑232)	pnp → / → rnj2	Polyribonucleotide nucleotidyltransferase/Ribonuclease J 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,298,108	0	T	A	100.0%	221.4 / NA	68	intergenic (+4/‑232)	pnp/rnj2	Polyribonucleotide nucleotidyltransferase/Ribonuclease J 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (30/38);  total (30/38)

ACAAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATTTAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTATCCTGTA  >  USA300TCH1516_ALE/1297975‑1298244                                                                                                                                      |                                                                                                                                         accaacttCAAAAAATAGAATGGAAAAAGTGGAAGATGTATGAAAAATCGGTGACAAAATTGAAGCTAAGATTACTGAATTTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTc                                                                                                                                   <  1:226536/135‑1 (MQ=255) acaAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTc                                                                                                                                   >  2:181752/1‑141 (MQ=255) acaAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTc                                                                                                                                   <  1:838696/141‑1 (MQ=255)  caAAATTCAAAAAATAGAAATGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCa                                                                                                                                  <  1:603656/141‑1 (MQ=255)     attataaaaaaCAAAAATGAAAAAGTTGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCatat                                                                                                                               <  2:524987/136‑1 (MQ=255)     atcacAAAAAAAAGAAAAGAAACAGTGGAACATGTATTAAAAATCGTCGACACAAGTGAAGTTAAGATTACTGAAATGGATAAACAAGCTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCatat                                                                                                                               <  1:68923/137‑1 (MQ=255)          aaaaaaTAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATgacga                                                                                                                          <  1:912562/141‑1 (MQ=255)           aaaaaTAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATgacgac                                                                                                                         >  1:1048197/1‑141 (MQ=255)           aaaaaTAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATgacgac                                                                                                                         >  1:943149/1‑141 (MQ=255)                tAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGt                                                                                                                    >  2:958530/1‑141 (MQ=255)                    aTTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCg                                                                                                                <  2:49746/141‑1 (MQ=255)                    aTTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCg                                                                                                                >  1:115277/1‑141 (MQ=255)                    aTTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCg                                                                                                                >  1:623885/1‑141 (MQ=255)                          aaaGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCAtgt                                                                                                          <  1:1192619/141‑1 (MQ=255)                           aaGTGGAAGATGTATTAACCCTCATTCACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAATCATATGACGACAATGTATCGACAtgcg                                                                                                         >  2:550355/1‑139 (MQ=255)                           aaGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATAGTCAtgtg                                                                                                         >  2:1082153/1‑141 (MQ=255)                            aggggAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGa                                                                                                        <  1:1086220/138‑1 (MQ=255)                            aGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGa                                                                                                        <  1:894551/141‑1 (MQ=255)                              tGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGAtt                                                                                                      >  1:24621/1‑141 (MQ=255)                                 aaGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGAttttt                                                                                                   >  2:97511/1‑141 (MQ=255)                                      gTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATg                                                                                              <  1:478271/141‑1 (MQ=255)                                               atcagcgACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTAGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACACTGTATCGTCATGTGATTATTTTATGTAACttttt                                                                                     >  2:1031058/7‑141 (MQ=255)                                                        acaATTGAAGTTAAGAGTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGa                                                                            >  1:174398/1‑141 (MQ=255)                                                          aaTTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGAcc                                                                          <  1:1028823/141‑1 (MQ=255)                                                            ttGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCg                                                                        <  2:378211/141‑1 (MQ=255)                                                             tGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGt                                                                       >  1:326314/1‑141 (MQ=255)                                                              gAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGtt                                                                      <  1:1121039/141‑1 (MQ=255)                                                              gAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGtt                                                                      <  1:847856/141‑1 (MQ=255)                                                                aGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGtttt                                                                    >  2:1009178/1‑141 (MQ=255)                                                                aGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGtttt                                                                    >  1:895665/1‑141 (MQ=255)                                                                   tAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGGGCCTTAAAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGaa                                                                 <  1:925526/141‑1 (MQ=255)                                                                   tAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGaa                                                                 <  1:75129/141‑1 (MQ=255)                                                                    aaGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAAt                                                                <  2:24621/141‑1 (MQ=255)                                                                    aaGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTAACGTAATTTTGTTATGCGACGTTTTACGAAGTGACCCGTTTTGAAt                                                                >  2:248428/1‑141 (MQ=255)                                                                     aGATTACTGAAATTGATCAACAAATTCGAGTAAATGCTTCACATAGCGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAAtt                                                               <  1:508818/141‑1 (MQ=255)                                                                      aaTTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAAttt                                                              <  1:1157640/140‑1 (MQ=255)                                                                       aTTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATttg                                                             >  2:1018463/1‑141 (MQ=255)                                                                        ttACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATttgt                                                            <  1:958530/141‑1 (MQ=255)                                                                         tACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATttgtt                                                           <  2:474357/141‑1 (MQ=255)                                                                          aCTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTGATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATttgttg                                                          >  2:750892/1‑141 (MQ=255)                                                                             gAAATTGATAAACAAGGTCGAGCAAATGCTTCACATAGAGCACTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTAt                                                       <  1:29839/141‑1 (MQ=255)                                                                               aaTTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTAttt                                                     >  2:538990/1‑140 (MQ=255)                                                                                 ttGATAAACCAGGACGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATAGGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTaaa                                                   <  2:861780/141‑1 (MQ=255)                                                                                 ttGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGACTTTGTTGTATTGaa                                                   >  1:450130/1‑141 (MQ=255)                                                                                     tAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAAAAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACAtt                                               >  1:631131/1‑141 (MQ=255)                                                                                      aaaCAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGATTTTGTTGTATTGAACAttt                                              <  1:634508/141‑1 (MQ=255)                                                                                      aaaCAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACAttt                                              <  1:635118/141‑1 (MQ=255)                                                                                       aaCAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATATAGGCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACAtttt                                             >  1:930206/1‑141 (MQ=255)                                                                                       aaCAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACTtttt                                             >  2:113074/1‑141 (MQ=255)                                                                                       aaCAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACAtttt                                             >  2:992099/1‑141 (MQ=255)                                                                                         caagtgcGAGTAAATGCTTGACAAAGATCATTAGAAGAATAATATATAAAGTCATATTACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTaa                                           <  2:1172600/135‑1 (MQ=255)                                                                                            ggTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAAc                                        >  1:294933/1‑141 (MQ=255)                                                                                                 aGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCttt                                   >  1:27684/1‑141 (MQ=255)                                                                                                 aGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCttt                                   >  2:910215/1‑141 (MQ=255)                                                                                                 aGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCttt                                   >  1:846173/1‑141 (MQ=255)                                                                                                     caTGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGAGTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTGATATTGAACATTTTTAAACGCTtaattt                               >  2:493645/2‑140 (MQ=255)                                                                                                      aTGGTTCACATAGAGCATTAGAAGAATAATATATGAAGTCATATGACGACAATGTATTGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTtattat                              <  2:391281/141‑1 (MQ=255)                                                                                                          ttCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTtg                          <  2:162042/141‑1 (MQ=255)                                                                                                            cacaTAGAGTATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTtgtg                        <  1:130456/141‑1 (MQ=255)                                                                                                             acaTAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTtgtgt                       <  1:325208/141‑1 (MQ=255)                                                                                                                   agCATTAGAAGAATAATATATAAAGTCATATGACGACAATGCATCGTAATGTGATTTTTTTATGCCCTTGTTTACGAAGTTTCCAGTTTTTTTTTTGTTGTATTTAACATTTTAAAACTCTGTATTATTTTGTGTGCAACt                 >  2:104206/1‑141 (MQ=255)                                                                                                                    gCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATCATTTTGTGTGCAACTg                <  1:612517/141‑1 (MQ=255)                                                                                                                       ttAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTa             <  1:963307/141‑1 (MQ=255)                                                                                                                        tAGAAGTATAATATATAAAGTCATATGACGACCATGTTTTGTCATTTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTaa            <  1:513905/141‑1 (MQ=255)                                                                                                                         agaagaAAAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTCATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTACAACGCTTTATTATTTTGTGTGCAACTGTTAAt           <  2:13986/141‑1 (MQ=255)                                                                                                                           aagaaTAATATATAAAGTCATATGATGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTa         <  1:995685/141‑1 (MQ=255)                                                                                                                           aagaaTAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTa         <  1:176472/141‑1 (MQ=255)                                                                                                                            agaaTAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTAt        <  1:97511/141‑1 (MQ=255)                                                                                                                                  atatatAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTTACCCGTTTTGCATTTGTTGTATTGAACATTTTAACACGCTTTTTTATTTTTTGTGCACCTGTTAATTATCAtgtt  >  2:453173/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         ACAAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATTTAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTATCCTGTA  >  USA300TCH1516_ALE/1297975‑1298244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑