Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 1,624,673 T→A K192I (AAA→ATA)  malL ← Oligo‑1,6‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,624,6730TA98.8% 271.8 / NA 85K192I (AAA→ATA) malLOligo‑1,6‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (51/33);  minor base G (1/0);  total (52/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTGTATACCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTTTATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGATCCA  >  USA300TCH1516_ALE/1624538‑1624810
                                                                                                                                       |                                                                                                                                         
gTTTGTATACCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAGTTGCATCTACTCTAAATCCATCAATGCCTATATCa                                                                                                                                      >  1:671767/1‑141 (MQ=255)
     tataCCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAacca                                                                                                                                 <  1:737987/141‑1 (MQ=255)
             gCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTtca                                                                                                                         <  2:812511/141‑1 (MQ=255)
              cTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTCATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTtcat                                                                                                                        >  1:362723/1‑141 (MQ=255)
                 aTTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCCACTCTCAATCCATCAATGCCTATATCAAACCCCCAGTTcatcat                                                                                                                     >  2:97110/1‑141 (MQ=255)
                    cATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATGTCAAACCACCAGTTCATCATTTc                                                                                                                  >  1:554025/1‑141 (MQ=255)
                         cTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCAACTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATa                                                                                                             <  2:23640/141‑1 (MQ=255)
                          tACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATAc                                                                                                            <  2:709198/141‑1 (MQ=255)
                            cATCAAATGCTGGGGCATATGTTTTACCCTCAGGTAAAGGTAACTCACCGCCTTAATATGCCTTCTTAATAAGCGTATATATATTTAATGTAAGGTCTTCAATCCCCATATCCATCCCCCACTCCATCATTTCAAATACac                                                                                                          >  2:103754/1‑140 (MQ=255)
                              tCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCa                                                                                                        >  1:261707/1‑141 (MQ=255)
                              acAAATGCTGAGGCATATATTTAAACCTCAGGTACAGGTAAGGCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTCAATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCa                                                                                                        <  1:198607/140‑1 (MQ=255)
                                     cTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTaa                                                                                                 <  1:87940/141‑1 (MQ=255)
                                              atGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCACCATTTCAAATACAGCATCTCTAACTTCCGGAt                                                                                        >  1:1013361/1‑141 (MQ=255)
                                                  tttACCCTCCGGTACAGGTAAGTCCCCCGCTTCAACCGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTAcc                                                                                    <  1:1141278/141‑1 (MQ=255)
                                                  tttACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTAcc                                                                                    <  1:849537/141‑1 (MQ=255)
                                                  tttACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTAcc                                                                                    <  1:450831/141‑1 (MQ=255)
                                                      cccTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCaa                                                                                >  1:130088/1‑141 (MQ=255)
                                                      cccTCAGGTACAGGTAAGTCACCCGCTGCAAACGTCTTCTTAATGTGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCaa                                                                                >  1:641158/1‑141 (MQ=255)
                                                       ccTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAGATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAAt                                                                               >  1:164194/1‑141 (MQ=255)
                                                       ccTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCACATAAAGCCTCGCGAACTTCCGGATTACCCCAAt                                                                               <  2:1150230/141‑1 (MQ=255)
                                                       ccTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAAt                                                                               >  2:539203/1‑141 (MQ=255)
                                                       ccTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAAt                                                                               >  1:2934/1‑141 (MQ=255)
                                                       ccTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCAGCATTTCCAATACAGCATCTATAACTTCCGGATTACCCCAAt                                                                               >  1:29791/1‑141 (MQ=255)
                                                        cgcaggtacaggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACAACCAGTTAATCATTTAAAATACAGCATCTCTAACTTACGGTTTACCCCACtt                                                                              >  2:936089/3‑141 (MQ=255)
                                                        cTCGGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCCGATTACCCCACtt                                                                              >  2:935996/1‑141 (MQ=255)
                                                          caggtacaggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCCTATAACCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCa                                                                            >  1:35148/1‑141 (MQ=255)
                                                          caggtacaggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCCCCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCa                                                                            >  1:35444/1‑141 (MQ=255)
                                                           aggtacaggtaAGTCACCCGCTTCAAACGTCTTCTTACTATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCACTTCaa                                                                           >  2:1165363/1‑141 (MQ=255)
                                                            ggtacaggcaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAACTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCaaa                                                                          <  2:1058522/141‑1 (MQ=255)
                                                            gctacaggtaAGTCACCCGCTTCAAACGTCTTCTTAACACGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCaaa                                                                          <  2:867165/139‑1 (MQ=255)
                                                                caggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTGAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAg                                                                      >  1:476291/1‑141 (MQ=255)
                                                                caggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAg                                                                      >  1:50743/1‑141 (MQ=255)
                                                                caggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAg                                                                      >  2:527884/1‑141 (MQ=255)
                                                                caggtaAGTCACCCGCGTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCGCTGACTTCCGGATTCACCCAATTCAACTCAg                                                                      >  1:189814/1‑141 (MQ=255)
                                                                  ggtaAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGgt                                                                    >  1:493463/1‑141 (MQ=255)
                                                                  ggtaAGTCAACCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGgt                                                                    <  1:448633/141‑1 (MQ=255)
                                                                     aaGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAACTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGgttgt                                                                 <  2:518028/141‑1 (MQ=255)
                                                                      aGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGattgtt                                                                >  2:747624/1‑141 (MQ=255)
                                                                      aGTCACCCGCTTCAAACGTATTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCGAATCAGgttgtt                                                                >  2:669067/1‑141 (MQ=255)
                                                                       ttCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGttt                                                               <  2:812910/140‑1 (MQ=255)
                                                                       gTCACCCGCTTCAAACTTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCGATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGttt                                                               <  1:1179846/141‑1 (MQ=255)
                                                                          aCCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTCCTCTAACTCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGtttttt                                                            >  1:230291/1‑141 (MQ=255)
                                                                           cccGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAAGGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTACCTTCCGCATTCCCCCAATTCAAATCAGGTTGttttttt                                                           >  2:18629/1‑140 (MQ=255)
                                                                            ccGCTTCAAACGTCTTCTTAATATACGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTAc                                                          >  1:870017/1‑141 (MQ=255)
                                                                                ttCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGaa                                                      >  2:922191/1‑141 (MQ=255)
                                                                                   aaaCGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATaa                                                   >  1:1173457/1‑141 (MQ=255)
                                                                                     aCGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAAt                                                 >  1:437271/1‑141 (MQ=255)
                                                                                            cttAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAaata                                          >  1:419746/1‑141 (MQ=255)
                                                                                              tAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAaataat                                        <  2:458210/141‑1 (MQ=255)
                                                                                               aaTATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAataata                                       <  1:679046/141‑1 (MQ=255)
                                                                                                atatGCGTAACTGCATCTACACTAAATCCACTATTGCCTATATCAAACCACCAGGTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAatat                                      <  1:103754/141‑1 (MQ=255)
                                                                                                     cGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATCACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGCAAATAATATTGATc                                 >  2:988607/1‑141 (MQ=255)
                                                                                                      gTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCa                                <  1:539203/141‑1 (MQ=255)
                                                                                                         aTTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCAATTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCCGTa                             <  1:858248/141‑1 (MQ=255)
                                                                                                            gCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTa                          >  1:790009/1‑141 (MQ=255)
                                                                                                            gCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTa                          >  1:894976/1‑141 (MQ=255)
                                                                                                             cATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAg                         >  1:1051280/1‑141 (MQ=255)
                                                                                                             cATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAg                         >  1:379999/1‑141 (MQ=255)
                                                                                                               tCTACTCTAAATCCATCAATGCCTATATCAAAGCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATAAGGTTGTTTTTTACTGAATAAATGGAAGTAATATTACGAAGTATTTGca                       >  1:528004/1‑141 (MQ=255)
                                                                                                               tCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGca                       <  1:496980/141‑1 (MQ=255)
                                                                                                               tCTACCCTAACTCCATCAATTCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGca                       <  1:43457/141‑1 (MQ=255)
                                                                                                                cTATTCTACATCCACCAACGCCTAGATCAAACCACCCGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGcat                      <  2:701420/141‑1 (MQ=255)
                                                                                                                cTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCTGTATTAGcat                      <  2:807453/141‑1 (MQ=255)
                                                                                                                  aCTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGcatca                    >  2:194026/1‑141 (MQ=255)
                                                                                                                   ctctAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGcatcat                   >  2:1135155/1‑141 (MQ=255)
                                                                                                                     ctAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCatat                 <  1:984662/141‑1 (MQ=255)
                                                                                                                     ctAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCatat                 >  1:1091707/1‑141 (MQ=255)
                                                                                                                     ctAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTACTCAGTATTAGCATCatat                 >  1:900937/1‑141 (MQ=255)
                                                                                                                      tAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACCGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCGGTATTAGCATCATAtt                >  1:702714/1‑141 (MQ=255)
                                                                                                                       aaaTCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTc               >  2:697502/1‑141 (MQ=255)
                                                                                                                        aaTCCATCAATGCCTGTATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATACATCAAAGAAAGAGTGCTCAGTATTCGCATCATATTcc              >  2:1140200/1‑141 (MQ=255)
                                                                                                                        aaTACATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGAGTCTGTAACTTCCGGATTACCCCAATTAAAATGAGGTTGTTTGTTACTGAATAAATGGAAATAATATTGCTAAGTATTAGTATCATATTcg              >  2:66578/1‑140 (MQ=255)
                                                                                                                          tCCATCAATGCCTATATCACACCACCAGTTCATCATTTCAAATACAGCATCTCTCACTTCCGGCTTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATACATGGAAATAATATTGCTCGGTCTTAGCATCATTTTTCCa            >  1:356692/1‑141 (MQ=255)
                                                                                                                          tCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCa            <  2:35444/141‑1 (MQ=255)
                                                                                                                          tCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCa            <  2:35148/141‑1 (MQ=255)
                                                                                                                          tCCATCAATGCCGATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGCTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCa            <  2:178521/141‑1 (MQ=255)
                                                                                                                            cATCAATGCCTATATCAAACCGGCAGTTCATCATTTCAAATACAGAATCTATAACTTCCGCATGACCACAATTCAAATCAGTTTGTTTTTTACTGAATAAATGGAAATAATATTGCTGAGTATTAGCATCATATTGCTATg          >  2:602514/1‑141 (MQ=255)
                                                                                                                            cATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATg          <  2:671767/141‑1 (MQ=255)
                                                                                                                              tgtaTGCCTATATCGAGCCACCAGTTCATCATTTCATATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTTCTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTa        <  2:228912/138‑1 (MQ=255)
                                                                                                                              tCAATGCCTATATCAAACCACCAGTGCATCGTTGCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTAAATACATGGCAGTAATGTTGCTCAGTATTAGCCTCAGATTCCGATGTa        >  2:599557/1‑141 (MQ=255)
                                                                                                                               cAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAg       >  2:1143163/1‑141 (MQ=255)
                                                                                                                                aaTGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGa      >  2:769900/1‑141 (MQ=255)
                                                                                                                                aaTGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGa      >  2:1112759/1‑141 (MQ=255)
                                                                                                                                 aTGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGAt     <  2:1108908/141‑1 (MQ=255)
                                                                                                                                    ccTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGATCCa  <  1:527884/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                         
GTTTGTATACCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTTTATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGATCCA  >  USA300TCH1516_ALE/1624538‑1624810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑