BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	99.0%	352.4 / NA	102	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (55/46); minor base A (1/0); total (56/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 102 total aligned reads displayed.

TTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATAT  >  USA300TCH1516_ALE/1646052‑1646322

ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                     <  2:392478/141‑1 (MQ=255)

    aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                 >  2:972843/1‑141 (MQ=255)

       cctgtcAACCCCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTTCTTCTTTTCCAGTCATGCCTCCTTTTacaac                                                                                                                              <  1:22342/136‑1 (MQ=255)

       aCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaac                                                                                                                              <  2:962164/141‑1 (MQ=255)

           gCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGtt                                                                                                                          <  1:77586/141‑1 (MQ=255)

           gCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAAtt                                                                                                                          >  2:329221/1‑141 (MQ=255)

             aaCACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑aa                                                                                                                       >  1:949668/1‑141 (MQ=255)

             aaCACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑aa                                                                                                                       <  1:11654/141‑1 (MQ=255)

               cacCATGTCGTAATGCACCTCTATCACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCATTCATGCCTCCTTTTACAACAGTT‑aaaa                                                                                                                     <  2:15561/141‑1 (MQ=255)

                    tgtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTCATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTGTACAACAGTT‑AAAatatat                                                                                                                <  2:825440/141‑1 (MQ=255)

                      tgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTc                                                                                                              <  1:1002509/141‑1 (MQ=255)

                           aTGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATg                                                                                                         >  1:65400/1‑141 (MQ=255)

                           aTGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATg                                                                                                         <  2:126933/141‑1 (MQ=255)

                            tGCACCTCTATGTCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGt                                                                                                        <  1:152162/141‑1 (MQ=255)

                             gCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGtt                                                                                                       >  1:289844/1‑141 (MQ=255)

                             gCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGtt                                                                                                       <  2:1040651/141‑1 (MQ=255)

                              cACCTCTATGACCAGATGCTACAATCGTCTCGAATGATTGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTTAAACAATT‑AAAATATGTTCATATGttt                                                                                                      >  1:45923/1‑141 (MQ=255)

                               aCCTCTATGACCAGATGCTACCATCGTATCGAATGATGGTCCATGTGCTACTAAGTCTAGCATTTTGATTTCTAATATTTCCTTGACTTCTTTGTCAGTGATGCCTCCTTTTCCAACAGTTGAAGATA‑ATTCATATGttgg                                                                                                     >  2:519503/1‑139 (MQ=255)

                               aCCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTACTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATAAATTCATATGttta                                                                                                     >  2:519515/1‑140 (MQ=255)

                                ccTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTtca                                                                                                    <  2:459835/141‑1 (MQ=255)

                                ccTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTtca                                                                                                    >  1:457020/1‑141 (MQ=255)

                                      tGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATcaaca                                                                                              >  2:372000/1‑141 (MQ=255)

                                      tGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATcaaca                                                                                              >  1:972579/1‑141 (MQ=255)

                                      tGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATcaaca                                                                                              >  1:1138872/1‑141 (MQ=255)

                                          cAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAatat                                                                                          >  1:419761/1‑141 (MQ=255)

                                             aTGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCGTATGTTTCATCAACAATATTAg                                                                                       >  1:398394/1‑141 (MQ=255)

                                             aTGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAg                                                                                       >  1:13556/1‑141 (MQ=255)

                                              tGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAgc                                                                                      <  2:972012/141‑1 (MQ=255)

                                              tGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTGTTTCAGTCATGCCTCCTTTTACAGCAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAgc                                                                                      <  2:367492/141‑1 (MQ=255)

                                                cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTCCAACAGTT‑AAAATATATTGCTATGTTTCCTCAACAATAttgactg                                                                                    >  2:1029023/1‑136 (MQ=255)

                                                cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACACCAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAgctg                                                                                    >  1:289605/1‑141 (MQ=255)

                                                cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATACCTCCTTTTACAACAGTT‑AAAATATATTCTTATTTTTATTCAACACTATTaaatg                                                                                    >  1:87264/1‑137 (MQ=255)

                                                  aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAgctgct                                                                                  >  1:1027288/1‑141 (MQ=255)

                                                   cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCtt                                                                                 <  2:854775/141‑1 (MQ=255)

                                                   cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCtt                                                                                 <  2:51614/141‑1 (MQ=255)

                                                          aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTCGCTGCTTTTTGAAt                                                                          >  1:963287/1‑141 (MQ=255)

                                                          aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAt                                                                          >  2:1011895/1‑141 (MQ=255)

                                                           tCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAtt                                                                         <  1:972843/141‑1 (MQ=255)

                                                           tCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAtt                                                                         >  2:1039067/1‑141 (MQ=255)

                                                            cGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTGTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTAATCCACCATATAAGCTGCTTTTTGAATTa                                                                        >  2:618315/1‑141 (MQ=255)

                                                                  atgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATCTTAGCTGCTTTTTGAATCATAGCat                                                                  >  1:825324/1‑140 (MQ=255)

                                                                    ggTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAtt                                                                >  1:959325/1‑141 (MQ=255)

                                                                    ggTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAtt                                                                >  1:1193938/1‑141 (MQ=255)

                                                                    ggTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAtt                                                                >  1:312606/1‑141 (MQ=255)

                                                                          tCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACGATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCa                                                          >  2:268296/1‑141 (MQ=255)

                                                                                    actcgaGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGa                                                >  2:733917/6‑141 (MQ=255)

                                                                                          gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGATGCTTTTTGAATTAAAGCAAATTCGTCGGCCTTGTTGACGtctc                                          <  2:699017/141‑1 (MQ=255)

                                                                                           cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTctct                                         <  1:571301/141‑1 (MQ=255)

                                                                                           cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCACCATCTTTGACGTctct                                         <  1:709114/141‑1 (MQ=255)

                                                                                                    ttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAATCAATTTCGTCAGCATCTTTGACGTCTCTACTTTTAtt                                >  2:409994/1‑140 (MQ=255)

                                                                                                       tAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAACTATATTCATCTGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCGATTTCGTCAGCATCTTTGACGACTCTAACTTTATCttc                             >  2:42019/1‑139 (MQ=255)

                                                                                                            ttGCCTTTAATTCTTTTTCAGTCATGGCTACTTTTACAACAGTT‑AAAATATATTCATGTGTTTAATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCGGCATCTTTGACGTCTCTAATTGTATCTATAGttt                        >  1:1141603/1‑139 (MQ=255)

                                                                                                            ttGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTATATTTATCTACAGTAt                        >  2:479662/1‑141 (MQ=255)

                                                                                                            ttGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAt                        <  2:949668/141‑1 (MQ=255)

                                                                                                             tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                       <  2:567413/141‑1 (MQ=255)

                                                                                                             tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                       <  2:920876/141‑1 (MQ=255)

                                                                                                             tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                       <  2:1061735/141‑1 (MQ=255)

                                                                                                             tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                       <  2:503201/141‑1 (MQ=255)

                                                                                                             tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                       <  2:951627/141‑1 (MQ=255)

                                                                                                             tGCCTTTAATTCTTGTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                       <  2:598607/141‑1 (MQ=255)

                                                                                                              gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTa                      >  2:74548/1‑141 (MQ=255)

                                                                                                              gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTa                      <  2:972579/141‑1 (MQ=255)

                                                                                                              gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTa                      >  2:977858/1‑141 (MQ=255)

                                                                                                              gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTa                      <  2:1138872/141‑1 (MQ=255)

                                                                                                               ccTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAg                     <  1:439147/141‑1 (MQ=255)

                                                                                                                cTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGa                    >  1:786526/1‑141 (MQ=255)

                                                                                                                 tttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                   >  1:352090/1‑141 (MQ=255)

                                                                                                                  ttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaaa                  >  2:687515/1‑141 (MQ=255)

                                                                                                                  ttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATATTTGACATTTCTTATTTTATTTTCAGTAtttgcaa                  >  2:1076628/1‑136 (MQ=255)

                                                                                                                   tAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAAt                 <  2:266043/141‑1 (MQ=255)

                                                                                                                   tAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAAt                 <  1:1039067/141‑1 (MQ=255)

                                                                                                                   tAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAAt                 <  2:578628/141‑1 (MQ=255)

                                                                                                                   tAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAAt                 <  2:266138/141‑1 (MQ=255)

                                                                                                                     aTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaatggc               >  1:304057/1‑137 (MQ=255)

                                                                                                                     aTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc               >  1:939859/1‑141 (MQ=255)

                                                                                                                     aTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc               >  1:415712/1‑141 (MQ=255)

                                                                                                                      ttCTGTTTCCGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCt              <  2:190048/141‑1 (MQ=255)

                                                                                                                       tCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtt             <  2:941703/141‑1 (MQ=255)

                                                                                                                       tCTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtt             >  1:691601/1‑141 (MQ=255)

                                                                                                                       gctgtttCAGTCATTGCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtt             >  2:261904/5‑141 (MQ=255)

                                                                                                                        cTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtta            >  1:684121/1‑141 (MQ=255)

                                                                                                                        cTTTTTCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTAATATGTTTCATCAACAATATTAGCTGCTTTTTAAATTAAAGCAATTTCGTCAGCGTCTTTGACGGCTCTAATTTTATTTACAGGTTTCGAAATGcaga            >  1:488725/1‑138 (MQ=255)

                                                                                                                          ttttCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTACTTTTATTTGAAGTATTAGAAATGCttatt          >  2:590966/1‑141 (MQ=255)

                                                                                                                           tttCAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatta         >  1:1191637/1‑141 (MQ=255)

                                                                                                                           tttCAGTCATGCCTCATTTTACAACAGTT‑AAAATATACTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatta         >  1:885442/1‑141 (MQ=255)

                                                                                                                              cAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTCTTAGAAATGCTTATTAATg      >  1:353808/1‑141 (MQ=255)

                                                                                                                              cAGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATAAACAATATTAGCTGCTTTTTAAATTAAAGCAATTTCGTCAGAATCTTTGACGTATAAAATTTTGTATCGAGTGTTAGAAATTCTTATTAATg      >  2:1123623/1‑141 (MQ=255)

                                                                                                                               aGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCTACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCCGCAACTTTGACGNCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa     <  2:661696/141‑1 (MQ=255)

                                                                                                                               aGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa     <  2:959325/141‑1 (MQ=255)

                                                                                                                               aGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa     <  2:1193938/141‑1 (MQ=255)

                                                                                                                               aGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa     <  1:597269/141‑1 (MQ=255)

                                                                                                                               aGTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa     <  2:135350/141‑1 (MQ=255)

                                                                                                                                gTCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATgtt    >  2:659691/1‑139 (MQ=255)

                                                                                                                                 tCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGGATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGata   >  2:580386/1‑141 (MQ=255)

                                                                                                                                 tCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCCTCTTTGCAGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGata   <  2:197259/141‑1 (MQ=255)

                                                                                                                                 tCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGata   <  2:918023/141‑1 (MQ=255)

                                                                                                                                 tCATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAAGGata   >  2:905975/1‑141 (MQ=255)

                                                                                                                                 tCACGCCTCCTTTTAAAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAATGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAAGGCTTATTAATGata   <  2:889335/141‑1 (MQ=255)

                                                                                                                                  cATGCCTCCTTTTACCACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGatat  >  2:100840/1‑141 (MQ=255)

                                                                                                                                  cATGCCTCCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTTTTTTATTTCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTTCAGTATTAGAAATGCTTATTACTGatat  >  2:965036/1‑141 (MQ=255)

TTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTT‑AAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATAT  >  USA300TCH1516_ALE/1646052‑1646322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`