Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,761,889	C→A	D388Y (GAT→TAT)	fpgS ←	Folylpolyglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,761,889	0	C	A	100.0%	305.8 / NA	93	D388Y (GAT→TAT)	fpgS	Folylpolyglutamate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (51/42);  total (51/42)

TCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGT  >  USA300TCH1516_ALE/1761756‑1762022                                                                                                                                      |                                                                                                                                      tcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAAt                                                                                                                                <  2:201745/141‑1 (MQ=255) tcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAAt                                                                                                                                >  2:778041/1‑141 (MQ=255)  cATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAAGTTTTTATAAATTCAACGTAGACATATACTAAtt                                                                                                                               >  2:670884/1‑141 (MQ=255)    tGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGt                                                                                                                             >  1:180026/1‑141 (MQ=255)       cTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTAtt                                                                                                                          <  1:748923/141‑1 (MQ=255)       cTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTAtt                                                                                                                          <  1:169022/141‑1 (MQ=255)       cTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTCGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTAtt                                                                                                                          <  1:654205/141‑1 (MQ=255)        tATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTTTAAATTCAACGTAGTCATATACTAATTGTAttt                                                                                                                         >  2:669503/1‑141 (MQ=255)        tATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTTTAAATTCAACGTAGTCATATACTAATTGTAttt                                                                                                                         >  2:311618/1‑141 (MQ=255)        tATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTAttt                                                                                                                         >  2:286397/1‑141 (MQ=255)              agttttagttttATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTcatcat                                                                                                                   >  2:78262/1‑141 (MQ=255)                 tttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTAAGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCAtttt                                                                                                                <  2:1015097/141‑1 (MQ=255)                  ttagttttaTAACGCTTCAAAGTCTAATTTTGATTCAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTc                                                                                                               <  1:14244/141‑1 (MQ=255)                   tagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCa                                                                                                              >  2:340122/1‑141 (MQ=255)                    agttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCaa                                                                                                             <  1:970495/141‑1 (MQ=255)                       tttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATATTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAAtac                                                                                                          >  2:658024/1‑141 (MQ=255)                           taATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActacc                                                                                                      <  1:598010/141‑1 (MQ=255)                           taATGCTTCAAAGTCTAATTTTGATTTAACTTCACTGATGAAATACAGACGACCGGTAATTACTAATGTATCACCTTGATAATCTTTTATAAATTCAACGTAATCTTATCATAATGGTATTTCTACGATTTCAAACctacc                                                                                                      >  2:631346/1‑141 (MQ=255)                             aTGCTTCAAAGTCTAATTTTGATTTAACTCCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActaccta                                                                                                    <  1:1098889/141‑1 (MQ=255)                              tGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATTATTTTTACGTCGTAATATACTAATTGTATTTCATAATTTTCAATActacttat                                                                                                   >  2:293480/1‑140 (MQ=255)                              tGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCACCGTAGTCATATACTAATTGTATTTCATCATTTTCAATActacctac                                                                                                   <  1:40862/141‑1 (MQ=255)                              tGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActacctac                                                                                                   >  2:1054018/1‑141 (MQ=255)                              agCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActacctac                                                                                                   <  2:1059789/140‑1 (MQ=255)                                  tCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAAtt                                                                                               <  1:534828/141‑1 (MQ=255)                                      aGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATttctt                                                                                           >  1:959811/1‑141 (MQ=255)                                      aGTCTAATTTTGATTTAACTTCACTTATGAAATAAAAGCTCCCGGTAAGTACTACAGTATCACCTTGATAAATTTTTATAAGTTCAACGCATGCATATACTAGTTTTATTTCATCATTTTCAATACTACCTACAATttctt                                                                                           >  2:492560/1‑141 (MQ=255)                                       gTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATttcttc                                                                                          <  2:180026/141‑1 (MQ=255)                                        tCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGGATCACCTTGATAATTTTTTATAAGTTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTtcttct                                                                                         >  2:657492/1‑141 (MQ=255)                                           aaTTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTg                                                                                      <  1:652571/141‑1 (MQ=255)                                             ttttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCg                                                                                    <  2:475889/141‑1 (MQ=255)                                              tttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGt                                                                                   >  2:843683/1‑141 (MQ=255)                                              tttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTAATATACTAATTGTATTTCATCATTTCAAATATTGCCTACAATTTCTTCTTTGCGt                                                                                   >  2:834385/1‑141 (MQ=255)                                                ggATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTaa                                                                                 <  1:297/140‑1 (MQ=255)                                                   tttAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAAcac                                                                              >  2:1073917/1‑139 (MQ=255)                                                   tttAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGc                                                                              >  1:503302/1‑141 (MQ=255)                                                   tttAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGc                                                                              >  1:663110/1‑141 (MQ=255)                                                   tttAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGc                                                                              >  2:353075/1‑141 (MQ=255)                                                     tAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCtt                                                                            <  1:78262/141‑1 (MQ=255)                                                       aCTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTc                                                                          >  1:377051/1‑141 (MQ=255)                                                         ttCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCgg                                                                        <  2:894736/141‑1 (MQ=255)                                                         ttCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCgg                                                                        <  1:991346/141‑1 (MQ=255)                                                         ttCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCgg                                                                        <  1:1098817/141‑1 (MQ=255)                                                                aTGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCa                                                                 >  2:1073682/1‑141 (MQ=255)                                                                aTGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCa                                                                 <  1:367424/141‑1 (MQ=255)                                                                  gAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCaaa                                                               <  1:343167/141‑1 (MQ=255)                                                                    aaTACAGCCTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAAtt                                                             <  1:713666/141‑1 (MQ=255)                                                                    aaTACAGACTACCGGTAATTACTAATTTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAAtt                                                             <  2:99708/141‑1 (MQ=255)                                                                    aaTACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAAtt                                                             <  2:862512/141‑1 (MQ=255)                                                                       aCAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAg                                                          >  1:696183/1‑141 (MQ=255)                                                                       aCAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAg                                                          >  1:1082730/1‑141 (MQ=255)                                                                        ccggctgccGGTAAGTACTAATGTATCACCTTTATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGt                                                         <  1:409793/134‑1 (MQ=255)                                                                        cAGACTACCGGTTATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCAGCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGt                                                         <  1:189854/141‑1 (MQ=255)                                                                        cAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCACTACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGt                                                         <  1:461809/141‑1 (MQ=255)                                                                         aGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGtt                                                        <  1:340122/141‑1 (MQ=255)                                                                            cTACCGGTAATTACTAATGTATCAGCTTGATAATTTTTTATAAATTCAACGTAGGCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCACATTCATTTGcg                                                     >  2:748868/1‑140 (MQ=255)                                                                               ccGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTCATTTTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACTCTTTCGGAAAATCGAATTCAGttagctca                                                  >  2:577763/1‑135 (MQ=255)                                                                                 ggTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATaaaa                                                >  2:1012750/1‑141 (MQ=255)                                                                                 ggTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATaaaa                                                >  2:1108199/1‑141 (MQ=255)                                                                                  gTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAAc                                               >  1:850760/1‑141 (MQ=255)                                                                                  gTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAAc                                               >  1:283139/1‑141 (MQ=255)                                                                                    aaTTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAGCGCTTTCGGGAAATCAAATTCAGTTACATAAAAAGt                                             >  1:151920/1‑141 (MQ=255)                                                                                    aaTTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGt                                             >  2:961802/1‑141 (MQ=255)                                                                                        aCTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgc                                         <  1:695984/141‑1 (MQ=255)                                                                                         cTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgcg                                        <  2:1042632/141‑1 (MQ=255)                                                                                         cTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgcg                                        >  1:605456/1‑141 (MQ=255)                                                                                             tttATCCCCTTGATAATTTTTTATAATTTCCACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAAt                                    <  1:272417/139‑1 (MQ=255)                                                                                             tGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAAt                                    <  2:1168414/141‑1 (MQ=255)                                                                                                   aCCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTaaaat                              >  1:1194841/1‑139 (MQ=255)                                                                                                      ttGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATTGGAAAGTACACTTaa                           >  2:30838/1‑141 (MQ=255)                                                                                                      ttGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTaa                           >  2:829653/1‑141 (MQ=255)                                                                                                        gATAATTTTTTATTAATTTTTCGTAGTCATATACTAATTGTATTTAATAATTTTTAATACTACTTAAAATTTATTATTTGCGTAACGCTTTCGGAAAATCAAATTTAGTTGCATCACATGTATGGGCAATTAACCTTCAAt                         >  2:943783/1‑141 (MQ=255)                                                                                                        gATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTACGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAAt                         >  1:490450/1‑141 (MQ=255)                                                                                                        gATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTAATCATTTTCACTCCGACGTACAATTTCTTCATTCCGTGTTTCTCACTATAAATGATATCCATTTGCATAAAACTTATGCCCAATTAAACTTAAAt                         >  2:544237/1‑141 (MQ=255)                                                                                                         aTAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAGAACGTATGCGCAATTAAACTTAAATg                        >  1:402373/1‑141 (MQ=255)                                                                                                          tcaTTTTTCATTAATTCTATGTCTACATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGt                       <  2:1123329/139‑1 (MQ=255)                                                                                                          tAATTTTTTATAAATTCAACGTAGTCATATACTTATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTCCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAAATCAAATGt                       >  2:328224/1‑141 (MQ=255)                                                                                                          tAATTTGTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGt                       >  2:77224/1‑141 (MQ=255)                                                                                                              tttttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGa                   <  2:186737/141‑1 (MQ=255)                                                                                                              tttttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGa                   <  2:3615/141‑1 (MQ=255)                                                                                                                 ttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATATAACGTATGCGGAATTAAACTTAAATGTTTGACCa                >  1:1135485/1‑141 (MQ=255)                                                                                                                 ttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCa                >  2:1128981/1‑141 (MQ=255)                                                                                                                     aaaTTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtctc            >  1:100335/1‑141 (MQ=255)                                                                                                                      aaTTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCg           >  1:1125734/1‑141 (MQ=255)                                                                                                                      aaTTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCg           >  2:474258/1‑141 (MQ=255)                                                                                                                        ttCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGtt         >  1:24769/1‑141 (MQ=255)                                                                                                                            aCGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATc     <  1:123119/141‑1 (MQ=255)                                                                                                                              gTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACCATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCgg   <  2:163315/141‑1 (MQ=255)                                                                                                                              gTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCgt   >  1:75425/1‑140 (MQ=255)                                                                                                                              gTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCgg   <  1:1184365/141‑1 (MQ=255)                                                                                                                              gTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTACGTAACGCTTTCGGAAAATCAAATTCAGTTGCAGAAAACTTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCgg   >  2:544015/1‑141 (MQ=255)                                                                                                                               tAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGt  <  1:1108199/141‑1 (MQ=255)                                                                                                                               tAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGt  <  1:1012750/141‑1 (MQ=255)                                                                                                                               tAGTCATATACTAATTGTATTTCATCATTTTCAACACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTACATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAAGCGGt  <  1:1048283/141‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGT  >  USA300TCH1516_ALE/1761756‑1762022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑