Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,851,260	C→A	A115S (GCG→TCG)	acsA_1 ←	Acetyl‑coenzyme A synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,851,260	0	C	A	100.0%	281.0 / NA	92	A115S (GCG→TCG)	acsA_1	Acetyl‑coenzyme A synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (53/39);  total (53/39)

TACTCGAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGCAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTC  >  USA300TCH1516_ALE/1851123‑1851389                                                                                                                                          |                                                                                                                                  tACTCGAGGTAACAATGCCTTATTAGTAATTAAAACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGaaaa                                                                                                                                >  2:462453/1‑141 (MQ=255)  aCTCGAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAaaaa                                                                                                                               >  2:928443/1‑141 (MQ=255)     cGAGGTAACAATGCATTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATGACGGCCCAACAATTGCCCCAATTTTTAAACCACCTAACAACGAaaaaact                                                                                                                            >  2:819214/1‑138 (MQ=255)      gAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATGTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATGTTTAAAACACCTAACAACGAAAAAtata                                                                                                                           <  2:658386/141‑1 (MQ=255)      gAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACACATTTTTAAAACACCTAACAACGAAAAAtata                                                                                                                           >  2:362628/1‑141 (MQ=255)      gAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCAGAAATGCTTCAACTAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAAtata                                                                                                                           <  1:459078/141‑1 (MQ=255)      caGGTAACAATGCCTTACTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTCCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAAtata                                                                                                                           <  2:942482/140‑1 (MQ=255)             cAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAgg                                                                                                                    <  2:268386/141‑1 (MQ=255)             cAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAgg                                                                                                                    <  1:962919/141‑1 (MQ=255)             cAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAgg                                                                                                                    >  2:417141/1‑141 (MQ=255)                tGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACCATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGgtgt                                                                                                                 <  1:1055125/141‑1 (MQ=255)                   cTTATTAGTAATTAACACTTTAGCTTCACTTTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTAcg                                                                                                              <  2:612286/141‑1 (MQ=255)                           tAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAGAATACAGTTCAGGTGTACGCGACataa                                                                                                      >  1:736324/1‑141 (MQ=255)                            aaTTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCCCCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaa                                                                                                     >  2:564833/1‑141 (MQ=255)                             aTTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaat                                                                                                    <  1:970967/141‑1 (MQ=255)                               tAACACTTTAGCTTCACTGTTCTCTACTCTATCCGCTACTGCCTTTTCCATAACTGCTTCAAATACCGGCCCAACAATTGCACCAATTTTTAAAACACCTAGACACGAAAAATATAGTTCAGGCGTACACGACataaataa                                                                                                  >  2:340842/1‑140 (MQ=255)                               tAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaatat                                                                                                  <  1:621955/141‑1 (MQ=255)                               tAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAAAACCTAACACCGAAAAAAATAGTTCAGATGTACGCTACataaatat                                                                                                  >  2:479807/1‑141 (MQ=255)                                aaCACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGGGTACGCAGCataaatata                                                                                                 >  1:704835/1‑141 (MQ=255)                                aaCACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGATGTACGCGACataaataaa                                                                                                 >  2:1187573/1‑139 (MQ=255)                                  cacTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaatataaa                                                                                               >  1:335866/1‑141 (MQ=255)                                  cacTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaatataaa                                                                                               >  1:237911/1‑141 (MQ=255)                                    cTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACAtaaaaataaata                                                                                             >  2:166434/1‑141 (MQ=255)                                     tttAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATAc                                                                                            <  2:977245/141‑1 (MQ=255)                                     cttAGCTTCACTGTTCTCTAATCTAACCGCAACTGCCTATTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATAc                                                                                            <  2:327384/140‑1 (MQ=255)                                      ttAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATAct                                                                                           <  2:1106910/141‑1 (MQ=255)                                       tAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATActc                                                                                          <  1:387263/141‑1 (MQ=255)                                         gCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTg                                                                                        >  1:93927/1‑141 (MQ=255)                                          cTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGt                                                                                       >  1:1015684/1‑141 (MQ=255)                                           ttCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTc                                                                                      >  1:1108155/1‑141 (MQ=255)                                           ttCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTc                                                                                      >  1:260149/1‑141 (MQ=255)                                           ttCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTc                                                                                      >  2:1056504/1‑141 (MQ=255)                                           ttCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTGCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTc                                                                                      >  1:232603/1‑141 (MQ=255)                                                 gTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCCCCttt                                                                                >  1:1064222/1‑141 (MQ=255)                                                 gTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCttt                                                                                >  1:545966/1‑141 (MQ=255)                                                    ctctAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTc                                                                             >  1:345171/1‑141 (MQ=255)                                                        aaTCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACt                                                                         <  1:1032724/141‑1 (MQ=255)                                                        aaTCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACt                                                                         <  1:304512/141‑1 (MQ=255)                                                        aaTCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACt                                                                         <  1:99792/141‑1 (MQ=255)                                                              tCCGAAACTGCCTATTCCATAAATGCTTAAAATAACGGCAAAAAAATTGCACCAATTTTTAAAACACAAACCAACGAAAAATATATATCCAATGTACGCTACATAAATATAAATCGCATGTCACTTATATCAACTTATgaa                                                                   >  2:344401/1‑139 (MQ=255)                                                                cGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATg                                                                 >  1:357787/1‑141 (MQ=255)                                                                  cAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGtt                                                               >  1:384465/1‑141 (MQ=255)                                                                  cAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGtt                                                               >  2:924641/1‑141 (MQ=255)                                                                  cAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGtt                                                               >  2:1109362/1‑141 (MQ=255)                                                                  cAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGtt                                                               >  2:260129/1‑141 (MQ=255)                                                                  cAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTGTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGtt                                                               >  1:795966/1‑141 (MQ=255)                                                                  cAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCAAACAATTGCACCAATTTTTAAAAAACCAAACAACGAAAAATATAGTTCATGTGTACGCGACATAAATATAAGTACTATGTCACCTTGGTCAACTTCTGCagagt                                                               >  1:246319/1‑137 (MQ=255)                                                                      tGCCTTTTCCATAAATGCTTTAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAACTACCCTGTCACCTTTGTCAAGTTCTGCATGTTCAGa                                                           >  1:747522/1‑141 (MQ=255)                                                                      tGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACACTTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGGCACCTTTGTCAACTCCTGCATGTTCAGa                                                           >  2:1181695/1‑141 (MQ=255)                                                                       gCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGAc                                                          >  1:755180/1‑141 (MQ=255)                                                                        ccTTTTCCATAAATGCTTCAAATACAGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACa                                                         >  2:1149278/1‑141 (MQ=255)                                                                        ccTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACa                                                         >  1:1011235/1‑141 (MQ=255)                                                                        ccTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACa                                                         >  1:283068/1‑141 (MQ=255)                                                                         cTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAAATTCTGCATGTTCAGACaa                                                        >  2:479786/1‑141 (MQ=255)                                                                          ttttCCATAAATGCTTCAAATAACGGCCCAACAACTGCATCAATTTGTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACaaa                                                       <  1:1111982/141‑1 (MQ=255)                                                                            ttCCATAACTGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAAc                                                     <  1:550649/141‑1 (MQ=255)                                                                              ccATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCCACTTTTTCATGTTCAGACAAAACAt                                                   <  2:1152172/141‑1 (MQ=255)                                                                              ccATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACAt                                                   <  2:300652/141‑1 (MQ=255)                                                                                aTAACTGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTc                                                 <  2:521822/141‑1 (MQ=255)                                                                                aTAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTc                                                 >  2:778204/1‑141 (MQ=255)                                                                                 tAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCg                                                <  2:956781/141‑1 (MQ=255)                                                                                  aaaTGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCgc                                               >  1:1125281/1‑141 (MQ=255)                                                                                  aaaTGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCgc                                               <  1:152473/141‑1 (MQ=255)                                                                                       cTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCtt                                          >  1:298787/1‑141 (MQ=255)                                                                                        ttCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCATGTGTACGCGACACAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCttt                                         >  1:22177/1‑141 (MQ=255)                                                                                          cAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAGCGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTttat                                       >  1:931659/1‑141 (MQ=255)                                                                                          cAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATAAAGATCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTCCTGCATGTTCAGACAAAACAATCGCTGCTttat                                       >  2:543536/1‑141 (MQ=255)                                                                                            aaTACCGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTttatta                                     <  1:666153/141‑1 (MQ=255)                                                                                            aaTAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTttatta                                     >  2:602060/1‑141 (MQ=255)                                                                                              aaaCGGCCCAACCATTGCACCAATTTTTAAAACACCTACCAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGa                                   <  2:921840/140‑1 (MQ=255)                                                                                              aaaCGGCCCAACAACTTCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGa                                   <  2:674297/140‑1 (MQ=255)                                                                                               atcGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAAGATAGTTCAGGGGTACGCGACATAAATATAAATACTCCGTCACCTTTGTGTACTGCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGAt                                  <  2:8814/139‑1 (MQ=255)                                                                                               aaCGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGAt                                  <  2:435290/141‑1 (MQ=255)                                                                                                aCGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATa                                 <  2:77679/141‑1 (MQ=255)                                                                                                 cGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATaa                                <  2:815612/141‑1 (MQ=255)                                                                                                 cGGCCCAACAATTGCACCAATTTTTAAAACACCAACCAACCAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATaa                                <  1:248006/141‑1 (MQ=255)                                                                                                    cccAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCg                             >  1:946190/1‑141 (MQ=255)                                                                                                    cccAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCg                             >  2:937507/1‑141 (MQ=255)                                                                                                    cccAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCg                             >  2:832057/1‑141 (MQ=255)                                                                                                    cccAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCg                             >  1:1157720/1‑141 (MQ=255)                                                                                                        acaaTTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGc                         <  2:617030/141‑1 (MQ=255)                                                                                                        acaaATGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGc                         <  2:644680/141‑1 (MQ=255)                                                                                                          aaTTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCat                       >  1:1062502/1‑141 (MQ=255)                                                                                                           aTTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCata                      <  1:502531/141‑1 (MQ=255)                                                                                                            ttGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCatat                     <  2:357787/141‑1 (MQ=255)                                                                                                             tGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTGGATAACCGTTGCATATc                    >  2:572448/1‑141 (MQ=255)                                                                                                              gCACCAGTTTTTAAAACGCCGAACAACGAAAAATATAGTCGAGGTGATCGCGACATAAATATAAATACTCTGTCACCTTTGTTAACTTCTGCATGTTCAGACAAAACATTCGGTGATTTATTCGACCACCGTTGCATATCt                   <  2:275932/141‑1 (MQ=255)                                                                                                                aCCAATTTTTAAAACACCTAACAACGAAAAATATAGTTGAGGTGTACGCGACATAAATATAAGTAATCTGTCACCTTTGTCAACTACTGCATGTTAAGACAAACCATTCGCTGCTTTATTATATAACCGTTGCATATCttt                 >  2:1008376/1‑141 (MQ=255)                                                                                                                aCCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCCTATCttt                 >  2:122518/1‑141 (MQ=255)                                                                                                                aCCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCctt                 >  1:540140/1‑141 (MQ=255)                                                                                                                          aaaaCTCCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAATTACTCTGTCACTTTTGTCAACTTCTGCATGTTAAGACAAAACATTCGTTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATAc       <  2:418666/141‑1 (MQ=255)                                                                                                                          aaaaCACCCAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACACAACAATCCCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATAc       <  1:631255/141‑1 (MQ=255)                                                                                                                               cccTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCCTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTc  <  1:819214/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  TACTCGAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGCAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTC  >  USA300TCH1516_ALE/1851123‑1851389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑