| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,963,768 | C→G | V365L (GTA→CTA) | hemY ← | Protoporphyrinogen oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,963,768 | 0 | C | G | 98.1% | 360.5 / NA | 107 | V365L (GTA→CTA) | hemY | Protoporphyrinogen oxidase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (60/45); minor base T (2/0); total (62/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 107 total aligned reads displayed.
TACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCA > USA300TCH1516_ALE/1963630‑1963898 | tACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTCTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGAt < 2:98231/141‑1 (MQ=255) cTTTTCGGCAAACGATTGAAAATAAAACAATCACGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATAc > 1:285832/1‑141 (MQ=255) tttCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGc < 1:68163/141‑1 (MQ=255) tCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGAGCATCAACCACAGTATCAA‑CTGGTTTTCCTAGATCCGCAc > 2:487071/1‑141 (MQ=255) ggCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGa > 1:416035/1‑141 (MQ=255) aaaCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATc < 1:1195477/141‑1 (MQ=255) gATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCaaaa > 1:1011381/1‑141 (MQ=255) agaagtGTAAAGTCAGGATCACCTATATGTGTCATCATTCGACCCAAAGGTCGGCGTACAGGCGAGACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAacctta < 2:778216/136‑1 (MQ=255) cAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAaccttac < 1:1009773/141‑1 (MQ=255) cAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAaccttac > 1:963129/1‑141 (MQ=255) cAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAaccttac < 1:87305/141‑1 (MQ=255) cAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAaccttac < 1:383451/141‑1 (MQ=255) cAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAaccttac < 1:1193947/141‑1 (MQ=255) cAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAaccttac < 1:127270/141‑1 (MQ=255) cAATTGAAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAaccttac > 2:751708/1‑141 (MQ=255) aaaTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAaccttac < 2:423132/140‑1 (MQ=255) aaaTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAaccttac < 2:839609/140‑1 (MQ=255) aTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAaccttacct > 2:851866/1‑141 (MQ=255) ttGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAaccttacctt > 1:378216/1‑141 (MQ=255) tGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTc < 2:1120833/141‑1 (MQ=255) aaaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTCGATACGCACGAATCAAAACCTTACCTTCCgg > 1:376134/1‑141 (MQ=255) aaaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCgg > 2:483269/1‑141 (MQ=255) aaaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAAGCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCgg > 2:777295/1‑141 (MQ=255) gATAACCTTTAAATGTCATCATTTGACTTAAACCTCTACGTACAATCGATACTAATTCATCATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAACCCTTACCTTCCGgtgacgtga > 2:1033095/1‑135 (MQ=255) ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGc > 1:336044/1‑141 (MQ=255) aaTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAtttt > 2:28990/1‑141 (MQ=255) aaTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAtctt > 2:711236/1‑141 (MQ=255) aaTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGACCAtttt > 1:983666/1‑141 (MQ=255) aTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAttttt < 1:851866/141‑1 (MQ=255) tcatcaTTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCg > 2:620840/1‑141 (MQ=255) catcatTTGACTTCACCCTCCACGTACCACCCATACTAATTCTTTATCTTTATGATCATCAACCACGTTATCAC‑CTGGTTTGGCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa < 2:526479/141‑1 (MQ=255) catcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATCCGCACGCATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa < 1:1122721/141‑1 (MQ=255) tcacTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTATCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATg < 1:176020/141‑1 (MQ=255) atTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATTTc > 2:673497/1‑141 (MQ=255) ttGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATAAGCACGAATCAAACCCTTACCTTCCGGTGTAGTAAATGGCCCTTTTGTCGATGTACa > 2:1085371/1‑141 (MQ=255) tGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGCATCAAAACCGGACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCaa > 1:682021/1‑141 (MQ=255) gACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAg > 1:480863/1‑141 (MQ=255) gACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAg < 2:966654/141‑1 (MQ=255) aCTTAAATCTCTACGTACAATCGATACTAAGTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTCACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGt < 1:704013/141‑1 (MQ=255) cTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTa < 1:265496/141‑1 (MQ=255) tAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACa < 2:383635/141‑1 (MQ=255) tAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCAAAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTAACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACa < 2:387300/141‑1 (MQ=255) aaacttctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACAt < 2:749246/136‑1 (MQ=255) aaTCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATg > 1:100460/1‑141 (MQ=255) tctcTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCg < 2:183881/141‑1 (MQ=255) ctctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCgg > 2:1148573/1‑141 (MQ=255) ctctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCgg > 2:1025211/1‑141 (MQ=255) tctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTAAATGCGGt < 1:751708/141‑1 (MQ=255) tACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTaa > 2:1059734/1‑141 (MQ=255) cGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATg > 2:1041974/1‑141 (MQ=255) cGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATg > 1:752235/1‑141 (MQ=255) gTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGt > 1:999005/1‑141 (MQ=255) gTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGt > 1:150953/1‑141 (MQ=255) cAATCGATACTAATTCATTATCTGTATGATCATCAACCCAAGTA‑CACACCGGCTCACCTAGAAACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTACAAGTACATGCGGTAATGTCtt > 2:135596/1‑140 (MQ=255) aaTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTACTTTCTga > 2:588896/1‑140 (MQ=255) aaTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGt < 2:412333/141‑1 (MQ=255) aTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTa > 1:1185718/1‑141 (MQ=255) aTCGATACGAATTCATTATCTGTATGATCATCAACCACAGTATCCC‑CTGGTTTCACTAGATACGCCCGCATCAACACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTa > 2:448214/1‑141 (MQ=255) gATACTAATTCCTTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa < 2:583289/141‑1 (MQ=255) gATACTAATTCATTATCTGTATGATCATCAGCCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa < 1:298350/141‑1 (MQ=255) gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa > 1:949268/1‑141 (MQ=255) aCTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTc > 1:1058433/1‑141 (MQ=255) aCTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATACGGTAATCTCTATATCACTc > 1:1058422/1‑141 (MQ=255) aTTCCTTATCTGTATGATCATCAACCACAGTATCCC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCTGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTtc < 1:166649/141‑1 (MQ=255) aTTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTtc > 2:441214/1‑141 (MQ=255) cttctCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGc < 1:528751/137‑1 (MQ=255) aTTATCTGTATGATCATCAACCACAGTACCAC‑CCGGTTTACCTGGATACGCACGAACCAAAACCTTACCTCCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGc < 1:28330/141‑1 (MQ=255) aTCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCCAt > 2:1066369/1‑141 (MQ=255) aTCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAt > 1:204371/1‑141 (MQ=255) aTCTGTATGATCATCAACCACAGTATCAC‑CTGGTATACCTAGATACGAGGGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGAGGTAATATCTGTATCACTCGTTCTCGCAAt > 2:842434/1‑141 (MQ=255) tCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAtt > 2:908118/1‑141 (MQ=255) gTATGATCATCAACCACAGTAGCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTCCCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACg < 2:1011381/141‑1 (MQ=255) tATGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTAAGATTTACACGTTCTCGCAATTACGa < 2:550915/141‑1 (MQ=255) tATGATCATCAACCACAGTAGCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTCCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGa > 1:937073/1‑141 (MQ=255) aTGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGaa < 1:76938/141‑1 (MQ=255) tGATCATCAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAGTTACGAAg > 2:601807/1‑141 (MQ=255) tGATCATCAACCACAGAAAACC‑AAGGGGTACCTATATACGCACGAATCAAAACCTTACCTTCCGGTGAAGTAAATGGCCAAGTTATCGCGGTCCAAGTACATGCGGTAAAGTCTGTATCACTCGTTCTCGCAATCACGAAg > 1:286487/1‑141 (MQ=255) atcatcAACCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCTGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGcc < 1:766678/141‑1 (MQ=255) catcaGCCACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCACAACCTTACCTTCCGGTGTTGTAAATGGCCTTTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAg < 2:425433/141‑1 (MQ=255) ccACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcat > 1:674261/1‑141 (MQ=255) ccACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcat > 1:859766/1‑141 (MQ=255) ccACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcac > 1:1184728/1‑140 (MQ=255) ccACAGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGGGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGAAATTACGAAGCCAGTACcat > 2:911510/1‑141 (MQ=255) ccACAGTATCAC‑CTAGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCACTACcat > 2:811493/1‑141 (MQ=255) cacaGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACCTGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAGGCCGGTGCcatc > 1:246959/1‑141 (MQ=255) acaGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATGACGAAGCCAGTACcatca > 2:925111/1‑141 (MQ=255) acaGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTGCTCCCAATTACGAAGCCAGTACcatct > 1:351978/1‑140 (MQ=255) cggaaTCAC‑GTGGTTTACATATATACGCACGAATCAAAACCTAACCTTCCGCTGTAGTAGCTTGCCATTATTTCGATTTCCAACTACCTGCGGTAGTGCCAGTATCACTCCTCATCGCAATTACGAAGCCAGTAAcatcca > 2:308913/5‑139 (MQ=255) caGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatcat > 2:17320/1‑141 (MQ=255) aGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATCGCCATTTTTTCGATGTCCAAGTACGTGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg < 1:772813/141‑1 (MQ=255) aGTATCAC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTCCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg < 1:160967/141‑1 (MQ=255) gaTCAC‑CTGGTTTACCTAGGTACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATggg < 2:37890/140‑1 (MQ=255) cc‑cTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAtt < 1:938236/140‑1 (MQ=255) aC‑CTGGTTTACGTAGATACGCACGAATGCAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTTTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAtt < 2:711995/141‑1 (MQ=255) aC‑CTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAtt < 2:220534/141‑1 (MQ=255) tGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTc < 2:378216/141‑1 (MQ=255) cggTTTACCTAGATACGCCCGAATCAAACCCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTc < 1:32646/140‑1 (MQ=255) ggTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGCTGTCCAAGTACATGCGGTAACGTCAGTCTCCTGCAGTTTCGCGATTCGGAAGCCAGTACCATCATGGGTATTTTCa > 1:563509/1‑141 (MQ=255) ggTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTAGCAATTACGAAGCCAGTACCATCATGGGTACTTTCa > 2:51603/1‑141 (MQ=255) ggTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGATCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCa > 2:608121/1‑141 (MQ=255) | TACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCAC‑CTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCA > USA300TCH1516_ALE/1963630‑1963898 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |