BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,018,612	G→A	R103* (CGA→TGA)	mgt ←	Monofunctional glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,018,612	0	G	A	100.0%	327.7 / NA	102	R103* (CGA→TGA)	mgt	Monofunctional glycosyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (53/49); total (53/49)

Only 100 of 104 total aligned reads displayed.

CGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAA  >  USA300TCH1516_ALE/2018476‑2018749

cGATCATTATCATTAAAATAATTTTTAACAATTCGTTATGTAATGGTACTAGCACATTGCACAACGCAGTAGCTAATCGCTGAACATAAAGCTCTAGTTGTACCTTTCAAATCGAATCAATGATGATTGTATGATCATTCa                                                                                                                                       >  2:174021/1‑141 (MQ=255)

cGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCa                                                                                                                                       >  1:904034/1‑141 (MQ=255)

 gATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAAtcattcat                                                                                                                                      >  2:961643/1‑141 (MQ=255)

 gATCATTATCATAAAAATAATTTATGACAACTGGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAAtcattcat                                                                                                                                      >  1:286950/1‑141 (MQ=255)

  aTCATTATCATAAAAATAATTTTTGTCTACTATTTCCGTTATCGTTGTATCTCATAACACTTTTCTGTCACGGATCTTCTAAAATAAGCCTCTATGTTTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATc                                                                                                                                     <  2:103559/141‑1 (MQ=255)

  aTCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATc                                                                                                                                     <  2:387464/141‑1 (MQ=255)

    ctttcacATAAACATAATTTTTGACACTTTGTGTTGTAGTGGTGCTCCCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATATAGCTCTAGTTGTCCCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCtt                                                                                                                                   <  2:1072710/135‑1 (MQ=255)

    cATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCtt                                                                                                                                   >  2:755049/1‑141 (MQ=255)

    cATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCtt                                                                                                                                   >  2:150806/1‑141 (MQ=255)

     aTTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGCTGATTTTGGAATAATTAATCTTc                                                                                                                                  >  2:512325/1‑141 (MQ=255)

      ttATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTcc                                                                                                                                 >  2:623520/1‑141 (MQ=255)

       tATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCa                                                                                                                                >  1:980590/1‑141 (MQ=255)

       tATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCa                                                                                                                                >  2:906484/1‑141 (MQ=255)

              aaaaTTATTTTTGACAACTTTTTGTGTAATGTAACTTCCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAAt                                                                                                                         <  2:819307/141‑1 (MQ=255)

              aaaaTAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAAt                                                                                                                         <  1:1157767/141‑1 (MQ=255)

              aaaaTAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAAt                                                                                                                         <  1:401184/141‑1 (MQ=255)

                aataatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATaa                                                                                                                       <  2:280120/141‑1 (MQ=255)

                aataatTTTTGACAACGTGTTGTGTAATGGTACTACCACCTTGTACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATaa                                                                                                                       <  1:377011/141‑1 (MQ=255)

                 ataatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATaaa                                                                                                                      <  2:249882/141‑1 (MQ=255)

                 ataatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATaaa                                                                                                                      <  1:1094647/141‑1 (MQ=255)

                 ataatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTACTCTTCCATTGAATTaaa                                                                                                                      >  2:950371/1‑141 (MQ=255)

                   aatTTTTGACAACTTGTTGTGTAATGGTACTAGCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGCTGTACCGTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAgg                                                                                                                    <  1:338748/141‑1 (MQ=255)

                   aatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAgg                                                                                                                    >  1:850069/1‑141 (MQ=255)

                   aatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAgg                                                                                                                    >  1:755483/1‑141 (MQ=255)

                   aatTTTTGACAACTTGTTGTATAGTGGTCCTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGTTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAgg                                                                                                                    <  1:828463/141‑1 (MQ=255)

                     tttttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATGTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGGATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCa                                                                                                                  >  1:820030/1‑141 (MQ=255)

                     tttttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCa                                                                                                                  >  1:204778/1‑141 (MQ=255)

                       tttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCAcc                                                                                                                <  1:146650/141‑1 (MQ=255)

                              ccctgtcgtGTAATGCTACTACCCCCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAGGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACa                                                                                                         <  1:10514/138‑1 (MQ=255)

                              aCTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACa                                                                                                         <  2:619242/141‑1 (MQ=255)

                                ttgttgTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATa                                                                                                       <  1:882549/141‑1 (MQ=255)

                                 tgttgtGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAAccttt                                                                                                      <  2:904034/141‑5 (MQ=255)

                                    tgtgTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAACATAAAGCGCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATCctct                                                                                                   >  1:628948/1‑141 (MQ=255)

                                     gtgtAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTg                                                                                                  >  2:592728/1‑141 (MQ=255)

                                       gtAATGGTACTACCCCCTTGCACATCTCTGTCGCTAATCGTTGAACATAAAGCTCTAGATGTACCTTTCAAATCGAATCCATGATGTTTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGc                                                                                                <  2:563191/141‑1 (MQ=255)

                                       gtAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAGGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGc                                                                                                >  2:880260/1‑141 (MQ=255)

                                          aTGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGCAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAGCATACTCTGGCAtt                                                                                             >  1:853224/1‑140 (MQ=255)

                                               actacCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATc                                                                                        <  2:447437/141‑1 (MQ=255)

                                                   ccaccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCt                                                                                    >  1:227909/1‑141 (MQ=255)

                                                   ccaccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCt                                                                                    >  2:843724/1‑141 (MQ=255)

                                                   ccaccTTGCACATCGCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAACGGCACCTTTCACATACTCTGGCATGTTATCAGca                                                                                    >  2:813345/1‑140 (MQ=255)

                                                      ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTGGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCGTGTTATCAGCTggc                                                                                 >  1:249519/1‑139 (MQ=255)

                                                      ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCCCCTTTAACATACACTGGCATGTTGTCAGcgacc                                                                                 >  1:197411/1‑137 (MQ=255)

                                                      ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATATTATCAGCTGaa                                                                                 >  2:100846/1‑140 (MQ=255)

                                                      ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAAGCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGATTGTTATCAGCTGac                                                                                 >  1:348808/1‑141 (MQ=255)

                                                      ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTCCCAAATTGAATCCATGATGATGGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGac                                                                                 >  1:226959/1‑141 (MQ=255)

                                                          gCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCGGCTGACACaa                                                                             >  1:315272/1‑141 (MQ=255)

                                                          gCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACaa                                                                             >  1:806483/1‑141 (MQ=255)

                                                          gCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACaa                                                                             >  1:642739/1‑141 (MQ=255)

                                                            acaTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACaaaa                                                                           >  2:164985/1‑141 (MQ=255)

                                                               tctcTGTCGCTAATCGATGCAAATAAAGCTCTAGTTGTGCCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATGGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAacta                                                                        >  1:668953/1‑141 (MQ=255)

                                                                 tctGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAactact                                                                      >  2:1046173/1‑141 (MQ=255)

                                                                   tGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACttt                                                                    <  2:286950/141‑1 (MQ=255)

                                                                       gCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtt                                                                >  1:791644/1‑141 (MQ=255)

                                                                       gCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtt                                                                >  1:791520/1‑141 (MQ=255)

                                                                        cTACTCGTTGAAAATAAAGCTCTATTTGCACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                               <  1:219453/141‑1 (MQ=255)

                                                                          aaTCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTc                                                             >  2:899990/1‑141 (MQ=255)

                                                                             cGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTCTCAGCTGACACAAAACTACTTTTATTTTCAAt                                                          >  1:825070/1‑141 (MQ=255)

                                                                                  aaaaTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTc                                                     <  2:204778/141‑1 (MQ=255)

                                                                                    aaTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTAAATTTTTCtt                                                   >  1:239360/1‑141 (MQ=255)

                                                                                       aaaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGt                                                <  2:534098/141‑1 (MQ=255)

                                                                                        aaGCTCTAGTTGTTCCTTTCAAATCGAATCCATGATGATTGTAGAATCATCCATCTTCCATTGTAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTGTATTTTTAATTTTTCTTAGtt                                               <  2:818239/141‑1 (MQ=255)

                                                                                        aaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGtt                                               <  1:1089854/141‑1 (MQ=255)

                                                                                        aaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTACATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGtt                                               <  1:319349/141‑1 (MQ=255)

                                                                                            tctAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATc                                           >  2:810356/1‑141 (MQ=255)

                                                                                               aGTTGTACCTTTCAAATCGAATCCCTGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCcac                                        <  1:497116/141‑1 (MQ=255)

                                                                                               aGTTGTACCTTTCAAATCGAAGCCATGATGCTTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCcac                                        <  2:755769/141‑1 (MQ=255)

                                                                                                gTTGTACCTTTCAAATCGAATCCATGATGATTGTAGATTCATTCATCTTCCATTGAAACAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCcaca                                       <  2:218010/141‑1 (MQ=255)

                                                                                                   gTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACAttt                                    >  1:641196/1‑140 (MQ=255)

                                                                                                   gTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTa                                    >  1:735436/1‑141 (MQ=255)

                                                                                                   gTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTa                                    >  1:128454/1‑141 (MQ=255)

                                                                                                    tACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTAt                                   <  2:980590/141‑1 (MQ=255)

                                                                                                    tACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTAt                                   <  1:906484/141‑1 (MQ=255)

                                                                                                     aCCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATc                                  <  1:843724/141‑1 (MQ=255)

                                                                                                      cccttCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                                 <  1:471331/138‑1 (MQ=255)

                                                                                                      ccTTTCCAATCGAACCCGTGGTGCTTGTAGAATCATCCATCTTCCACTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                                 <  2:331690/141‑1 (MQ=255)

                                                                                                      ccTTTCAAATCGAATCCATGATGATTGGAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                                 <  2:193266/141‑1 (MQ=255)

                                                                                                           cAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGCAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCgtgt                            >  2:575528/1‑141 (MQ=255)

                                                                                                            aaaTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCCCATTATcgtgcgtt                           >  1:463955/1‑135 (MQ=255)

                                                                                                            aaaTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTa                           >  1:74906/1‑141 (MQ=255)

                                                                                                              aTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGa                         <  1:907200/141‑1 (MQ=255)

                                                                                                              aTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGa                         <  2:1192572/141‑1 (MQ=255)

                                                                                                              aTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGa                         <  1:715866/141‑1 (MQ=255)

                                                                                                               tCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCCCCTGTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACACTATCGCGTGTAGAt                        <  1:624917/141‑1 (MQ=255)

                                                                                                                cGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAACCTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATa                       >  2:778722/1‑141 (MQ=255)

                                                                                                                cGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATa                       >  2:436688/1‑141 (MQ=255)

                                                                                                                 gAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATaa                      >  1:229979/1‑141 (MQ=255)

                                                                                                                   aTCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTATTTCATCCACATTATCGCGTGTAGATaaaa                    <  2:1169210/141‑1 (MQ=255)

                                                                                                                    tCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATaaaaa                   >  2:237756/1‑141 (MQ=255)

                                                                                                                      cATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAt                 <  1:1046173/141‑1 (MQ=255)

                                                                                                                       attatgatTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa                <  2:351084/138‑1 (MQ=255)

                                                                                                                       atgatgATTGTAGAATCATTCATCTTCCATTGAAATAGAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa                <  1:1110919/141‑1 (MQ=255)

                                                                                                                       atgatgATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa                >  1:1179237/1‑141 (MQ=255)

                                                                                                                        tgatgaTTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATAc               >  2:1101111/1‑141 (MQ=255)

                                                                                                                               tGTATAATCATTCATATTCCAATGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCAAATAAAAAAAAAAAATACATGATAc        <  2:139077/141‑1 (MQ=255)

                                                                                                                                  aGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCaa     <  2:806483/141‑1 (MQ=255)

                                                                                                                                  aGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCaa     <  2:707179/141‑1 (MQ=255)

                                                                                                                                  aGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCaa     <  2:642739/141‑1 (MQ=255)

                                                                                                                                  aGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCaa     >  2:1172377/1‑141 (MQ=255)

                                                                                                                                     aTCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATaa  <  1:810356/141‑1 (MQ=255)

CGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAA  >  USA300TCH1516_ALE/2018476‑2018749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`