BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,352,815	G→A	A92V (GCA→GTA)	rpsI ←	30S ribosomal protein S9

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,352,815	0	G	A	99.2%	390.9 / NA	121	A92V (GCA→GTA)	rpsI	30S ribosomal protein S9
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (69/51); minor base C (1/0); total (70/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

ATATCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTAAA  >  USA300TCH1516_ALE/2352677‑2352949

atatCGTCCGACAATTACACTCTTGAGAATTGAGCTGAACGACCCGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGTGCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACt                                                                                                                                      <  1:102415/141‑1 (MQ=255)

 tatCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACtt                                                                                                                                     >  1:1093716/1‑141 (MQ=255)

   tCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGGCGAGTAGCCTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCt                                                                                                                                   <  1:221445/141‑1 (MQ=38)

         gACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGATGCTTTAAGAACTGGTTTTTTAAGTCCTTTAATACGTGGGCAACGAGGCAACAATCCAAGGCGTATTAACGAACCTCCGTATACAGCATCTCCTCTTaataat                                                                                                                             >  2:813050/1‑141 (MQ=12)

          aCAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATGTAATTCTAATAATg                                                                                                                            >  2:1138371/1‑141 (MQ=255)

          aCAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATg                                                                                                                            >  1:612000/1‑141 (MQ=255)

          aCAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTACATACGTGGGTCACGAGTAAGTAATCCCGCGCGTTTTAACGAACCTCTGTATTCAGGATCTACTTCTaataatc                                                                                                                            >  2:561674/1‑140 (MQ=255)

           cAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGACCCTCTGTATCCCGCATCTACTTCTAGTGATGc                                                                                                                           >  1:272948/1‑141 (MQ=37)

            aaTTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCa                                                                                                                          >  1:235483/1‑141 (MQ=255)

             aTTAACTTTTTGAGAATTGAGGAGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCAc                                                                                                                         <  1:753070/141‑1 (MQ=255)

             aTTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCag                                                                                                                         >  1:423050/1‑140 (MQ=255)

              ttAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACg                                                                                                                        >  2:1054073/1‑141 (MQ=255)

              ttAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTCTATGTTTTTTCATACGTGGGTTACGAGTAAGTCATCAAGAGCATTGTAAAGAACGTATGTATTTAGTATCTACTTATAATAATGCagg                                                                                                                        >  2:1083522/1‑139 (MQ=21)

               tAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTGTGCGTTCAGGATCTACTTCAAATAATGcgaga                                                                                                                       >  1:313666/1‑137 (MQ=38)

               tAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGCTCTACTTCTAATAATGCACGa                                                                                                                       >  2:656771/1‑141 (MQ=255)

                  cGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCg                                                                                                                    >  2:1105516/1‑141 (MQ=255)

                  cGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGGTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCg                                                                                                                    >  1:820730/1‑141 (MQ=255)

                  cGTTTTGAGAATTGACGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGGGCg                                                                                                                    >  1:886413/1‑141 (MQ=255)

                    ttttGAGAATTGAGGGGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGAt                                                                                                                  <  2:204859/141‑1 (MQ=255)

                          gaATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTg                                                                                                            >  2:352179/1‑141 (MQ=255)

                            aTTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGAc                                                                                                          >  1:1030289/1‑141 (MQ=255)

                             ttGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACg                                                                                                         >  1:1194004/1‑141 (MQ=255)

                                 ggTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCACGATCCACGTCTAATAATGCACGAGCGATTCCGTGACGGATa                                                                                                     >  1:808362/1‑141 (MQ=255)

                                    gAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGCTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGCGCGTTTCCGTGACGGATagct                                                                                                  <  1:825477/141‑1 (MQ=255)

                                     aaCGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATATGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagctt                                                                                                 >  1:675494/1‑141 (MQ=255)

                                        gacgaGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCTAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgag                                                                                              <  1:806306/141‑1 (MQ=255)

                                        gacgaGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgag                                                                                              <  1:1072905/141‑1 (MQ=255)

                                         acgaGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgagc                                                                                             <  2:155481/141‑1 (MQ=255)

                                          cgaTCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgagct                                                                                            <  1:1046446/141‑1 (MQ=255)

                                          cgaGCTGAGTTAAGTCTCGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgagct                                                                                            <  1:20502/141‑1 (MQ=255)

                                            aGCTGCTTTAAGACCTGGTTTTTTACGTTTTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgagcttg                                                                                          >  2:755383/1‑141 (MQ=255)

                                            aGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAGTAATGCACGAGCGATTCCATGACGGATagcttgagcttg                                                                                          >  2:558008/1‑141 (MQ=255)

                                             gctgctTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGt                                                                                         >  1:944900/1‑141 (MQ=255)

                                                    tAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGa                                                                                  <  2:235483/141‑1 (MQ=255)

                                                      aGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGGTTCCGTGACGGATAGCTTGAGCTTGTCCGGTGaaa                                                                                >  1:1122655/1‑141 (MQ=255)

                                                         ccTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAGCCTCTGTATTCAGGATCTACTTCTAATAATGCGCAAGCGATTCCGTGACGGATAGCTTGGGCTTGTCCAGTGAAacca                                                                             >  1:621545/1‑141 (MQ=255)

                                                           tGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccacc                                                                           >  2:14214/1‑141 (MQ=255)

                                                           tGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccacc                                                                           >  2:908622/1‑141 (MQ=255)

                                                           tGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccacc                                                                           >  2:14462/1‑141 (MQ=255)

                                                             gTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTAAGCGATTCCAGTGAAaccaccac                                                                         <  2:848656/141‑1 (MQ=255)

                                                                ttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTTCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCAt                                                                      >  1:564620/1‑141 (MQ=255)

                                                                  ttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGa                                                                    <  1:1071562/141‑1 (MQ=255)

                                                                  tgaCGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGa                                                                    <  1:1077785/139‑1 (MQ=255)

                                                                      gTTCTTTCATATGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGt                                                                <  2:1134567/141‑1 (MQ=255)

                                                                         cTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTaa                                                             <  2:171140/141‑1 (MQ=255)

                                                                          tttCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAAc                                                            <  1:342545/141‑1 (MQ=255)

                                                                          tttCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCCTGACGCCTAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAAc                                                            <  1:931962/141‑1 (MQ=255)

                                                                           tttaTACGTGGGTCACGAGTAAGTAGTCCGGCGCGTTTGAAAGAACGTCTGTATTCAGGAGCTACTTGTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACt                                                           <  2:633487/138‑1 (MQ=255)

                                                                           ttCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACt                                                           <  1:732149/141‑1 (MQ=255)

                                                                           ttCAGACGTGGGTCAAGAGTAAGTAATCCAGCGCGTTTTAAAGACCCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACt                                                           <  1:541472/141‑1 (MQ=255)

                                                                           tccATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACt                                                           <  1:372527/139‑1 (MQ=255)

                                                                             cATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAACGCAACTCTTCTTTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTaa                                                         <  1:388521/141‑1 (MQ=255)

                                                                             cATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTaa                                                         <  2:889775/141‑1 (MQ=255)

                                                                             cATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACAACCACCATGAAGGTTCAATaa                                                         >  2:295070/1‑141 (MQ=255)

                                                                               tACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTaaaa                                                       >  1:1139443/1‑141 (MQ=255)

                                                                                   tGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAcatc                                                   <  1:352179/141‑1 (MQ=255)

                                                                                    gggTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCCTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAcatca                                                  <  1:930988/141‑1 (MQ=255)

                                                                                         ccgtgtaagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGtt                                             <  2:835749/137‑1 (MQ=255)

                                                                                          cGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTa                                            >  1:162302/1‑141 (MQ=255)

                                                                                          cGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTa                                            >  1:1132460/1‑141 (MQ=255)

                                                                                           gAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTAc                                           >  2:38362/1‑141 (MQ=255)

                                                                                           gAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCAAAGTTAc                                           >  1:613184/1‑141 (MQ=255)

                                                                                             gtaagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGCAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCt                                         >  2:389383/1‑141 (MQ=255)

                                                                                             gtaagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCt                                         >  2:704103/1‑141 (MQ=255)

                                                                                             gtaagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATGCAGGATCTACTTCTGATAATGCACGAGCGATTCCGTGACGGATAGCTTGGGCTTGTCCAGTGGAACCACCACCATGAACGTTAAATAGAACATCATAGTTCCCt                                         >  2:125510/1‑141 (MQ=255)

                                                                                              taagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCtt                                        <  2:720939/141‑1 (MQ=255)

                                                                                               aagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCttt                                       <  1:908622/141‑1 (MQ=255)

                                                                                               aagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCttt                                       <  1:14214/141‑1 (MQ=255)

                                                                                               aagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCttt                                       <  1:14462/141‑1 (MQ=255)

                                                                                               aagtaaTCCAGCGAGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCttt                                       <  1:449003/141‑1 (MQ=255)

                                                                                                agtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATCGTTACCtttt                                      >  2:226386/1‑140 (MQ=255)

                                                                                                agtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTa                                      >  1:1157928/1‑141 (MQ=255)

                                                                                                agtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTa                                      >  1:1019741/1‑141 (MQ=255)

                                                                                                    aTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATATGTACCTTTacgtt                                  >  2:1098410/1‑137 (MQ=255)

                                                                                                          cgcgTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTTGAACCACCACCATGACCGTTAACTAAAACATCATAGTTACCTTTAGTTTAGGTTa                            >  1:115637/1‑141 (MQ=255)

                                                                                                             gTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATTAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACAt                         >  1:682174/1‑141 (MQ=255)

                                                                                                             gTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACAt                         >  1:792199/1‑141 (MQ=255)

                                                                                                               tttAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACAtga                       >  2:55443/1‑139 (MQ=255)

                                                                                                               tttAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACACCa                       >  2:1171845/1‑141 (MQ=255)

                                                                                                                 tAAAGAACCGCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCaaa                     <  1:971269/141‑1 (MQ=255)

                                                                                                                       aCCTTTGTTTTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttg               <  2:560105/141‑1 (MQ=255)

                                                                                                                       aCCTTTGTATTCAGGATCTACTTCTAATAATGCACGAGCCATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttg               <  2:86241/141‑1 (MQ=255)

                                                                                                                       aCCTCTGTATTCAGGATCTACTTCTCATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttg               <  2:1034298/141‑1 (MQ=255)

                                                                                                                       aCCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttg               <  1:130016/141‑1 (MQ=255)

                                                                                                                       aCCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttg               <  2:808362/141‑1 (MQ=255)

                                                                                                                       aCCTATGTAGTCAGTATCTACTTCTAATCATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttg               <  1:508511/141‑1 (MQ=255)

                                                                                                                        ccTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttgg              <  1:656771/141‑1 (MQ=255)

                                                                                                                            tGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTa          >  2:1080577/1‑141 (MQ=255)

                                                                                                                            tGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTa          >  2:336281/1‑141 (MQ=255)

                                                                                                                            tGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTAAATCAAACGGTTGGTTTa          >  2:110829/1‑141 (MQ=255)

                                                                                                                            tGTATTCAGGATCTACTTCTAATAATACACGAGAGATTCAGTGACGTATAGCTTAAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAATTCCCTTTAGTTTCCATTACATCAAAGGGTTGGTTTa          >  2:1089166/1‑141 (MQ=255)

                                                                                                                             gTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTaa         <  1:1012221/141‑1 (MQ=255)

                                                                                                                             gTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTaa         <  1:1011688/141‑1 (MQ=255)

                                                                                                                              tATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAg        >  2:509789/1‑141 (MQ=255)

                                                                                                                              tATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAg        >  1:905538/1‑141 (MQ=255)

                                                                                                                              tATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAg        >  1:395684/1‑141 (MQ=255)

                                                                                                                               aTTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGt       <  1:797282/141‑1 (MQ=255)

                                                                                                                                    ggATCTACTTCTAATAATGCTCGAGCGATTCCGTGACGGATAGCCTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAGATCATCATAGTTACCTTTAGTGTCAGTTACATCAAATGGTTGGTTTAAGTCTaaa  >  2:654013/1‑141 (MQ=255)

                                                                                                                                    ggATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCCTGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCACATGGACGTTTTATGTCCaaa  >  1:180695/1‑141 (MQ=255)

                                                                                                                                    ggATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATGATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGATTAAGTCTaaa  >  2:961831/1‑141 (MQ=255)

ATATCGTCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTAAA  >  USA300TCH1516_ALE/2352677‑2352949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`