Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 76,969 G→A intergenic (+509/‑107) USA300HOU_RS00140 → / → USA300HOU_RS00350 hypothetical protein/Monoacylglycerol lipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE76,9690GA100.0% 247.7 / NA 77intergenic (+509/‑107)USA300HOU_RS00140/USA300HOU_RS00350hypothetical protein/Monoacylglycerol lipase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/41);  total (36/41)

CTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTCAAA  >  USA300TCH1516_ALE/76832‑77104
                                                                                                                                         |                                                                                                                                       
ctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGt                                                                                                                                      <  1:427631/141‑1 (MQ=255)
          aCGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTtatat                                                                                                                            <  1:702662/141‑1 (MQ=255)
            ggATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAg                                                                                                                          >  1:846593/1‑141 (MQ=255)
            ggATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAg                                                                                                                          <  1:79323/141‑1 (MQ=255)
               ttttCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTCTATAGTAt                                                                                                                       >  2:1130110/1‑141 (MQ=255)
                  tCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTTCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATAAc                                                                                                                    >  2:238619/1‑141 (MQ=255)
                  tCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTCATTTATTTAGAATTat                                                                                                                    >  2:173445/1‑140 (MQ=255)
                    gCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGCACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTg                                                                                                                  >  1:327890/1‑141 (MQ=255)
                    gCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTACATAGTATTACTg                                                                                                                  >  1:764640/1‑141 (MQ=255)
                      cATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCa                                                                                                                <  1:102157/141‑1 (MQ=255)
                      cATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCa                                                                                                                <  2:470754/141‑1 (MQ=255)
                      cATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCa                                                                                                                <  1:550014/141‑1 (MQ=255)
                         gCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAAc                                                                                                             <  2:894521/141‑1 (MQ=255)
                          ccACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACa                                                                                                            <  1:737910/141‑1 (MQ=255)
                          ccACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACa                                                                                                            <  1:359294/141‑1 (MQ=255)
                           cACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACAt                                                                                                           <  1:690056/141‑1 (MQ=255)
                                  ttAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAGAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTg                                                                                                    >  1:370496/1‑141 (MQ=255)
                                  ttAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTg                                                                                                    >  1:767752/1‑141 (MQ=255)
                                  ttAGCATCATGCTAGCAAGTGAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTACATAGTATTACTGCAAACATTGATTTg                                                                                                    >  1:729018/1‑141 (MQ=255)
                                   tAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTAATACTGGAACCATTGATTTgg                                                                                                   >  1:565191/1‑141 (MQ=255)
                                   tAGCATCATGCGAGCAAGTTAAGCGAACTCTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAGCCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTgg                                                                                                   <  2:1014578/141‑1 (MQ=255)
                                    aGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTggg                                                                                                  <  1:65047/141‑1 (MQ=255)
                                      catcatGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTCTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTc                                                                                                <  1:129488/141‑1 (MQ=255)
                                        tcatGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCaa                                                                                              <  1:401774/141‑1 (MQ=255)
                                               aGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTGATAGAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAg                                                                                       >  2:694335/1‑141 (MQ=255)
                                                gCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAgg                                                                                      >  1:238426/1‑141 (MQ=255)
                                                     ttAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTAt                                                                                 >  1:1066540/1‑141 (MQ=255)
                                                          cGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaaa                                                                            >  1:505585/1‑141 (MQ=255)
                                                                cTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTCTTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAaatga                                                                      >  2:814034/1‑141 (MQ=255)
                                                                cTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAgatga                                                                      >  2:474129/1‑141 (MQ=255)
                                                                 tGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAaatgaa                                                                     >  2:1009722/1‑141 (MQ=255)
                                                                  gACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTCCTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAaatgaat                                                                    <  1:930663/141‑1 (MQ=255)
                                                                       gATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATc                                                               <  1:1151527/141‑1 (MQ=255)
                                                                           aaTTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATaa                                                           >  2:1130188/1‑141 (MQ=255)
                                                                                gcggatAGGTATATGTGGTTACGTGGGAACAGAAACTACACAAAACGTGGTCTTAAGATGGTAATTTAGATTGTATCACTGCAAACAGTGACTTGGGTCAAAAATTAGGGGTATTCAGAAAATGAATGAATCATCGTTTTa                                                      <  2:962889/136‑1 (MQ=255)
                                                                                gTGGTTAGCTATATTTTTTTACTTTTCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTa                                                      <  1:113317/141‑1 (MQ=255)
                                                                                  ggTTAGCTATATTATTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATc                                                    <  1:137773/141‑1 (MQ=255)
                                                                                     tAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAg                                                 <  1:265095/141‑1 (MQ=255)
                                                                                     tAGCTATAGTGTGTTACTATGCAACAGGACCTATCAAAAACATGGGCTTAAAACGTTAATTTATATAGTATTACTGCAAACATTAAAAAAAAAAAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAg                                                 <  2:632149/141‑1 (MQ=255)
                                                                                        cTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTg                                              >  1:989534/1‑141 (MQ=255)
                                                                                         tataTTTTTTTTTTTTTTTATAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATAGAGTATTACTGCGAACATTGATTTGGGTCAAGAAGTAGGGTTATTTATAATATTATTGAATCATAATTTTATAAAGCTgt                                             >  2:893136/1‑140 (MQ=255)
                                                                                         tataTTTTTTTACTTTGCAACAGAACCTATAAAAAACATAGGCTACAAAGATTAATTTATATAGTATTACTACACACCCAGCAAAGGGTCATACATTAGGGGGATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGa                                             >  2:365268/1‑141 (MQ=255)
                                                                                           tatTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGAtt                                           <  1:305584/141‑1 (MQ=255)
                                                                                            ttttttttACTTTGCAACAGCACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATAATTATTTTATCAAGCTGCTTg                                          <  1:1186410/140‑1 (MQ=255)
                                                                                                ttttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGgaga                                      >  1:427677/1‑141 (MQ=255)
                                                                                                  ttaaattGCAACAAAACCTCAAAAAAACACGGACTTAAAAGGATAATTTATATAGTATTACTGTAAACATGAAAAAAAAAAAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAgg                                    <  2:956559/136‑1 (MQ=255)
                                                                                                  ttACTTTGCAACAGGACCTATAAAAAACATGGGCGTAAAAGGTTAATTTATAGAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGGTTGGAGAgg                                    <  2:158723/141‑1 (MQ=255)
                                                                                                  ttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAgg                                    <  2:725216/141‑1 (MQ=255)
                                                                                                  ttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAgg                                    <  2:121578/141‑1 (MQ=255)
                                                                                                    aCTTTTCAACAGAACCTAGAAATAACATGGGCTTAAAAAGTTAATTTATATAGTATTACTGCAAACATTTATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGtt                                  <  1:537680/141‑1 (MQ=255)
                                                                                                    aCTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGtt                                  >  1:342981/1‑141 (MQ=255)
                                                                                                    aCTTTGCAACAGAACCCATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGtt                                  <  2:1077261/141‑1 (MQ=255)
                                                                                                     cTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTa                                 >  1:676696/1‑141 (MQ=255)
                                                                                                      tttGCAAGAGAACCTATAAAAAACATGGGCTTAAAAGGTTTATCTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGATACATAATTTTATCAAGCTGATTGGAGAGGTTaa                                <  2:202408/141‑1 (MQ=255)
                                                                                                      tgtGCAACAGAACCAAAAAAAAACAGGGGATTAAAAGGTAAAATAATATAGCAATACTGCAAACAATAAATTGGGTAAAAAAAAAAGAGAAATAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTaa                                <  2:299719/139‑1 (MQ=255)
                                                                                                        tGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAGGCTGATTGGAGAGGTTaaaa                              <  1:1134821/141‑1 (MQ=255)
                                                                                                        tGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTaaaa                              >  1:441947/1‑141 (MQ=255)
                                                                                                        tGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTaaaa                              <  1:831287/141‑1 (MQ=255)
                                                                                                              aGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCAtt                        >  2:745208/1‑141 (MQ=255)
                                                                                                               gAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATta                       >  2:869984/1‑141 (MQ=255)
                                                                                                               aaaCCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATta                       <  1:196563/140‑1 (MQ=255)
                                                                                                                    tgtaAAAAACATGGGCTTAAAAGGTTCATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATCTATTATATaa                  <  1:875413/139‑1 (MQ=255)
                                                                                                                    tataAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaa                  >  1:854250/1‑141 (MQ=255)
                                                                                                                     ataAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGTATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaa                 >  1:1156964/1‑141 (MQ=255)
                                                                                                                      taAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaaa                >  1:826975/1‑141 (MQ=255)
                                                                                                                      taAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaaa                >  1:827000/1‑141 (MQ=255)
                                                                                                                       gaaaaaCACGCGCATAAAATGTTAATTTATAGAGGATTACTGCAAACAGTGCTTAGGGTAAAAAATTCGGGCCATCAAAAAAATGAATGAATCATAATTTTAGCAAGCGGATTGGAGAGGTTAAATTGCATTATATAAAAt               <  2:403481/140‑1 (MQ=255)
                                                                                                                            aCATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTAtt          <  1:418262/141‑1 (MQ=255)
                                                                                                                             cATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTg         >  2:1069273/1‑141 (MQ=255)
                                                                                                                             cATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGAATTATATAAAATTTATTg         >  2:1153290/1‑141 (MQ=255)
                                                                                                                             cATGGGATTAAAAGGTTGATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTg         <  2:266725/141‑1 (MQ=255)
                                                                                                                               tGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAg       <  1:354666/141‑1 (MQ=255)
                                                                                                                                 ggCTTAAGAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGTATGGAGAGGTTAAACAGCATTATATAAAATTTATTAAGta     >  2:176013/1‑140 (MQ=255)
                                                                                                                                 ggCTTAACAGGTTTATGCATATAGTATTCCTCAAAACATTGACCTGCGTCAGAAATTAGGGATATTCCAAATATGCATGACTCATAACTTTATCAACCTCGTTGGAGAGGATAAAATGCATTATATAAAATTTCTTGAgac     >  2:215652/1‑139 (MQ=255)
                                                                                                                                 ggCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTCGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGGTTGGAGAGGTTAAAATGCATTATATAAACATAATTGAggc     >  1:1042546/1‑139 (MQ=255)
                                                                                                                                  gCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTCa    <  1:1009722/141‑1 (MQ=255)
                                                                                                                                    ttAAAAGGTTAATTTATATAGTATTACAGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTCaaa  <  1:694335/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTCAAA  >  USA300TCH1516_ALE/76832‑77104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑