BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	396,540	G→A	A208T (GCA→ACA)	efeM →	putative iron uptake system component EfeM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	396,540	0	G	A	100.0%	334.0 / NA	101	A208T (GCA→ACA)	efeM	putative iron uptake system component EfeM
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (56/45); total (56/45)

Only 100 of 103 total aligned reads displayed.

ATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGA  >  USA300TCH1516_ALE/396404‑396673

aTTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGcacaca                                                                                                                                   <  1:379703/141‑1 (MQ=255)

   aGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTACATGGAGTGACAACGTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACaaaa                                                                                                                                <  1:34431/141‑1 (MQ=255)

    gATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACaaaaa                                                                                                                               >  1:135566/1‑141 (MQ=255)

    gATATCATACCAAAATTAATATTACAAGGTTCTGTTGACCTATTGAATGAAGATGCAAATTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATCTGATTTTAAAGCGAACGTCGAAGGCACACaaaaa                                                                                                                               >  1:725708/1‑141 (MQ=255)

      tatCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAt                                                                                                                             <  1:413297/141‑1 (MQ=255)

      tatCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAt                                                                                                                             <  1:539072/141‑1 (MQ=255)

       atCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAtt                                                                                                                            >  1:1061512/1‑141 (MQ=255)

         cATACCAAAATTAAAGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTa                                                                                                                          >  1:626314/1‑141 (MQ=255)

          aTACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATCTATATACAAAGGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTAt                                                                                                                         >  1:91560/1‑141 (MQ=255)

          aTACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTAt                                                                                                                         <  2:360873/141‑1 (MQ=255)

            aCCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGa                                                                                                                       >  2:158862/1‑141 (MQ=255)

            aCCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGa                                                                                                                       >  2:598855/1‑141 (MQ=255)

             ccAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGAc                                                                                                                      >  1:831724/1‑141 (MQ=255)

              cAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACt                                                                                                                     <  1:215852/141‑1 (MQ=255)

              cAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACt                                                                                                                     <  2:1085409/141‑1 (MQ=255)

               aaaaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACtt                                                                                                                    <  2:597497/141‑1 (MQ=255)

                aaaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTa                                                                                                                   >  2:1059657/1‑141 (MQ=255)

                 aaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTAt                                                                                                                  <  1:278510/141‑1 (MQ=255)

                     aaTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaa                                                                                                              >  2:25064/1‑141 (MQ=255)

                     aaTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaa                                                                                                              <  2:825264/141‑1 (MQ=255)

                     aaTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGAAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaa                                                                                                              <  1:252733/141‑1 (MQ=255)

                      aTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                             >  1:23716/1‑141 (MQ=255)

                      aTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGACACACAAAAAATTTATGACTTATTTaaa                                                                                                             >  2:1112510/1‑141 (MQ=255)

                      aTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAAGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTCTTTaaa                                                                                                             >  2:92555/1‑141 (MQ=255)

                      aTGGTACAAGGTTATGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                             <  2:859811/141‑1 (MQ=255)

                           aCAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTAt                                                                                                        <  1:105202/141‑1 (MQ=255)

                               ggTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTa                                                                                                    <  2:679423/141‑1 (MQ=255)

                               ggTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATAGTTTTACTGTGATCTGTGAAGGCAAACAAAAAATTTATGACTTATTTAAACCTATTTTa                                                                                                    <  2:431529/141‑1 (MQ=255)

                                 ttCTGTTGACCTATTAAATGAATTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTACTATaga                                                                                                  <  2:614025/141‑1 (MQ=255)

                                 ttCTGTAGACCTATTAAATGAAGTTGCAACTTCTAAAATCGCAGGTGAAGAAGAAATTTATGCACATACAGATTTATATGATTTTAAAGCGAACGTTAAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTaga                                                                                                  <  2:529278/141‑1 (MQ=255)

                                  tCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTagag                                                                                                 >  1:206908/1‑141 (MQ=255)

                                    tGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                               >  2:420923/1‑141 (MQ=255)

                                       tGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaaa                                                                                            <  1:157556/141‑1 (MQ=255)

                                       tGAACTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaaa                                                                                            <  1:643280/141‑1 (MQ=255)

                                       ggccgtATTAAATGAAGTTGCAACTTCTAAACTCACAGGTTAAGAAGAAATTTATTCACATACAGATTCATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaaa                                                                                            <  2:759336/136‑1 (MQ=255)

                                        gACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaaaa                                                                                           >  1:989175/1‑141 (MQ=255)

                                         aCCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAg                                                                                          <  1:570721/141‑1 (MQ=255)

                                          ccTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGa                                                                                         >  1:189441/1‑141 (MQ=255)

                                          ccTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGa                                                                                         >  1:577156/1‑141 (MQ=255)

                                          ccTAATAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGTGAAAAAAGa                                                                                         >  2:828883/1‑141 (MQ=255)

                                              ttAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATaaa                                                                                     >  2:676468/1‑141 (MQ=255)

                                               tAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAATATaaaa                                                                                    <  2:662960/141‑1 (MQ=255)

                                                  aTGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAt                                                                                 <  1:1059657/141‑1 (MQ=255)

                                                    gAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTa                                                                               <  1:320061/141‑1 (MQ=255)

                                                       gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGt                                                                            >  2:351870/1‑141 (MQ=255)

                                                          gCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtgag                                                                         >  1:303097/1‑140 (MQ=255)

                                                            aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGGGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTGGTGTAGAGGAAAAAGATAAAAAATTAAGtgatga                                                                       >  1:922631/1‑141 (MQ=255)

                                                            aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtgatga                                                                       >  2:906768/1‑141 (MQ=255)

                                                              cTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATACAAAAGTAAGTGATGata                                                                     >  1:296434/1‑141 (MQ=255)

                                                                tCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATc                                                                   >  2:354326/1‑141 (MQ=255)

                                                                  tAAAATCAAAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAACCCTATTTTAGAGAAAAAAAAAAAAAAATTAAGTGATGATATCCa                                                                 <  2:865020/141‑1 (MQ=255)

                                                                  aaaaaGCACAGGTGAAGAATAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCa                                                                 <  2:611477/140‑1 (MQ=255)

                                                                   aaaaTCACCGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATCAAGTGATGATATCCaa                                                                <  1:593426/141‑1 (MQ=255)

                                                                   aaaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTCTGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCaa                                                                <  2:252977/141‑1 (MQ=255)

                                                                   aaaaTCACAGGTGAAGAAGAAATTTATTAACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCaa                                                                <  2:188602/141‑1 (MQ=255)

                                                                   aaaaTCACAAGTGAAGAAGAAATTTATTCACATACAGATTTATATGAGATTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCaa                                                                <  1:305700/141‑1 (MQ=255)

                                                                   aaaaTCAAAGGGTAAGAAGAAATTTATACAAATACAGATTTATAGGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCaa                                                                <  2:312346/141‑1 (MQ=255)

                                                                          caGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACt                                                         <  2:23716/141‑1 (MQ=255)

                                                                              tGAAGAAGAAATTTATTCACGTACAGATTTATATGATTTTAAAGCGGAGGTTGAAGGCAAACAAAAAATTTATGACTTATTTAAAACTATGTGAGAGAAAAAAGATAAAAAATTTAGTGATGATATCCGAATGAAcgtgga                                                     >  2:897450/1‑136 (MQ=255)

                                                                              tGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAGGTGAGGATATCCAAATGAACTTctg                                                     >  1:593650/1‑139 (MQ=255)

                                                                              agaaaaagaaATAAATTCACAGACAGATATATATAATTTTAAAGCGAACGTTGAAGGCAAACAAAAAATTTATGACTTATTTAAAAAAAAAAAAAAAAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGa                                                     <  2:956548/140‑1 (MQ=255)

                                                                                aagaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATa                                                   >  2:605075/1‑141 (MQ=255)

                                                                                aagaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATa                                                   <  2:937162/141‑1 (MQ=255)

                                                                                aagaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGAGATGTTATCAAAATGAACTTCGATa                                                   >  2:599130/1‑141 (MQ=255)

                                                                                  gaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATATCAGTGATAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATaaa                                                 >  1:331925/1‑141 (MQ=255)

                                                                                   aagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAg                                                <  1:144750/141‑1 (MQ=255)

                                                                                      aaaTTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGa                                             >  1:794551/1‑141 (MQ=255)

                                                                                      aaaTTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGa                                             >  1:945705/1‑141 (MQ=255)

                                                                                      aaaTTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGa                                             >  2:779246/1‑141 (MQ=255)

                                                                                       aaTTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGaa                                            <  1:1025561/141‑1 (MQ=255)

                                                                                       aaTTTATTCAAATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGaa                                            <  2:575296/141‑1 (MQ=255)

                                                                                          ttATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCa                                         >  1:400327/1‑141 (MQ=255)

                                                                                                    aCAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGAt                               <  1:116612/141‑1 (MQ=255)

                                                                                                       gATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATaaa                            <  1:931015/141‑1 (MQ=255)

                                                                                                        aTTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAat                           >  1:187115/1‑141 (MQ=255)

                                                                                                         tttAGATGATTTAAAAGCGGACGTTGAAGGCACACAAAAAATTTAGGAGTTATTTAAACCTAGTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTGCGAGAAAGTGAATCAATTATTGGATAAata                          <  2:24214/141‑1 (MQ=255)

                                                                                                         tgtataTGATTTAAAAGCGAACGTAGAAGGCACAAAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAata                          <  2:1132074/139‑1 (MQ=255)

                                                                                                         tatatataATTTTAAAGCGAACATTGAAGGAAAGAAAAAAATTTATGACTTATATAAACCTATTTTAGAGAAAAAAGATAAAAAATTAATTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAata                          <  2:654730/139‑1 (MQ=255)

                                                                                                              atGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAAtatacaa                     >  2:151634/1‑138 (MQ=255)

                                                                                                              atGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGAGTATCAATTATCGGCTAAATTGAAAg                     >  2:591614/1‑141 (MQ=255)

                                                                                                              atGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAAAATATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCAAAATGAACATCGATAAAGAGATTAAATTATTAgataaagataaaa                     >  2:1124948/1‑140 (MQ=255)

                                                                                                               tGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGa                    >  1:349260/1‑141 (MQ=255)

                                                                                                                  ttttAAAGCGAACGTTGAAGGAAGCCAAAAAAGTTAAGGCTCCCGTGATCCTGGGTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaa                 <  1:270686/141‑1 (MQ=255)

                                                                                                                   tttAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaac                <  1:911219/141‑1 (MQ=255)

                                                                                                                   tttAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaac                >  1:661811/1‑141 (MQ=255)

                                                                                                                      aaaGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaac             <  2:91560/141‑1 (MQ=255)

                                                                                                                       aaGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGCGAAAAAAGATAAAAGATTAAGTGATGATATCCAAATAAACTTCGATAAAGTAAATCAATTATTGGATAAATATAAAGATAACAAcg            >  1:61671/1‑141 (MQ=255)

                                                                                                                       aaGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAACAGATAAAAAATTAAGTGACGATACTTTAAGGAACATCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcg            >  1:1117460/1‑141 (MQ=255)

                                                                                                                        aGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATCAAAAATTAAGTGATGAAATCCAAATGAACTTCGATAAAGTGAATCAATGATTGGATAAATATAAAGATAACGAcgg           >  2:19096/1‑141 (MQ=255)

                                                                                                                        aGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcgg           >  2:962882/1‑141 (MQ=255)

                                                                                                                         gCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggc          >  2:473738/1‑141 (MQ=255)

                                                                                                                         gCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcgga          >  2:1046903/1‑140 (MQ=255)

                                                                                                                         gCGAACGATGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATACAAAGATAACAAcggc          >  1:746401/1‑141 (MQ=255)

                                                                                                                           gAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTATAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAACAATTGGAAAAATATAAAAAGAACAAcgacgg        >  2:686367/1‑141 (MQ=255)

                                                                                                                              cGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTa     >  2:453856/1‑141 (MQ=255)

                                                                                                                               gTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTAt    >  1:316662/1‑141 (MQ=255)

                                                                                                                                ttGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATAATTGGATAAATATAAAGATAACAACGGCGGTTATg   >  2:1124593/1‑141 (MQ=255)

                                                                                                                                 tGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGa  >  2:761896/1‑141 (MQ=255)

                                                                                                                                 tGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGa  >  1:367421/1‑141 (MQ=255)

                                                                                                                                 tGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGCGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGa  >  2:982309/1‑141 (MQ=255)

ATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGA  >  USA300TCH1516_ALE/396404‑396673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`