breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSNFR_1_104_1_S2366_L001_R1_001.good.fq1,196,080168,553,240100.0%140.9 bases141 bases98.9%
errorsSNFR_1_104_1_S2366_L001_R2_001.good.fq1,196,080168,553,240100.0%140.9 bases141 bases93.9%
total2,392,160337,106,480100.0%140.9 bases141 bases96.4%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionUSA300TCH1516_ALE2,872,915102.12.790.9%Staphylococcus species strain strain.
coveragedistributionCP00073127,041696.08.85.8%Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid
coveragedistributionNC_0124173,1253678.152.53.4%Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid
total2,903,081100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000009468
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000447
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.045

Junction Skew Score Calculation

reference sequencepr(no read start)
USA300TCH1516_ALE0.78068
CP0007310.29514
NC_0124170.06825

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input10:47:02 25 May 201910:47:36 25 May 201934 seconds
Read alignment to reference genome10:47:36 25 May 201910:53:36 25 May 20196 minutes 0 seconds
Preprocessing alignments for candidate junction identification10:53:36 25 May 201910:54:09 25 May 201933 seconds
Preliminary analysis of coverage distribution10:54:09 25 May 201910:55:37 25 May 20191 minute 28 seconds
Identifying junction candidates10:55:37 25 May 201910:55:48 25 May 201911 seconds
Re-alignment to junction candidates10:55:48 25 May 201910:57:32 25 May 20191 minute 44 seconds
Resolving best read alignments10:57:32 25 May 201910:58:29 25 May 201957 seconds
Creating BAM files10:58:29 25 May 201910:59:39 25 May 20191 minute 10 seconds
Tabulating error counts10:59:39 25 May 201911:00:04 25 May 201925 seconds
Re-calibrating base error rates11:00:04 25 May 201911:00:06 25 May 20192 seconds
Examining read alignment evidence11:00:06 25 May 201911:05:20 25 May 20195 minutes 14 seconds
Polymorphism statistics11:05:20 25 May 201911:05:20 25 May 20190 seconds
Output11:05:20 25 May 201911:05:32 25 May 201912 seconds
Total 18 minutes 30 seconds