Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,952,639 A→G 11.4% Y700Y (TAT→TAC nisB ← Nisin biosynthesis protein NisB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,952,6390AG11.4% 178.9 / 9.1 70Y700Y (TAT→TACnisBNisin biosynthesis protein NisB
Reads supporting (aligned to +/- strand):  ref base A (26/36);  new base G (4/4);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

ATGGCTTATCGATATGAATATGTAATGCAAACCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATAAATTTAGTAGAAGCTTGTTATCTCCATAAACT  >  USA300TCH1516_ALE/1952509‑1952777
                                                                                                                                  |                                                                                                                                          
aTGGCTTATCGATATGAATATGTAATGCAAACCAGTCTTTGTCATATTCAATATCTGTAATTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAAGAATTTCATACACAAGa                                                                                                                                   >  2:446412/1‑140 (MQ=255)
   gCTTATCGATATGAATATGTAATGCAAACCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTTGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCa                                                                                                                                <  1:419433/140‑1 (MQ=255)
       aTCGATATGAATATGTAATGCAAACCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCt                                                                                                                            <  2:343804/140‑1 (MQ=255)
        tCGATATGAATATGTAATGCAAACCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCtt                                                                                                                           >  2:226357/1‑140 (MQ=255)
              tGAATCTGTAATGCAAAACAGTCTTTGTCATATTCAATAACTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTGTAAATTGGTGTAACAATTTAATACACCTGATCATTCTtttattg                                                                                                                     >  2:129661/1‑140 (MQ=255)
              tGAATATGTAATGCAAACCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTttgattg                                                                                                                     >  1:514558/1‑140 (MQ=255)
               gAATATGTAATGCAAACCATTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATAAAAATGATCATTCTttgattga                                                                                                                    <  1:261031/140‑1 (MQ=255)
                      tAATGCAAACCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTaaaa                                                                                                             <  1:245078/140‑1 (MQ=255)
                        aTGCAAACCAGTCTTTGTCATATTGAATATCTTTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAAt                                                                                                           <  1:140847/140‑1 (MQ=255)
                         tGCAAACCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCGTACCCCTATTCATTCTTTGATTTAGGaaaacaac                                                                                                          >  2:136639/1‑136 (MQ=255)
                         tGCAAACCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCGTACACATGATCATTCTTTGATTGAGGTAAAAATg                                                                                                          >  1:528943/1‑140 (MQ=255)
                         tGCAAACCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTAGAGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCGTACACGTGATCGTTCTTTGAGTGAGGTAAAAATg                                                                                                          >  1:339893/1‑140 (MQ=255)
                         agcgaaCCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCGTACACATGATCATTCTTTGATTGAGGTAAAAATg                                                                                                          >  2:576640/5‑140 (MQ=255)
                             aaCCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAGATGAttt                                                                                                      >  2:552770/1‑139 (MQ=255)
                                   tCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGCTTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTaa                                                                                                >  1:93447/1‑140 (MQ=255)
                                            tatTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTTGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACg                                                                                       <  1:26159/140‑1 (MQ=255)
                                              ttCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGtt                                                                                     <  1:74236/140‑1 (MQ=255)
                                               tCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGttt                                                                                    <  2:561589/140‑1 (MQ=255)
                                               tCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGttt                                                                                    <  2:561534/140‑1 (MQ=255)
                                               tCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTACTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTAGAAAAAAAAAAAAATGATTCTACTAAACGTACACGttt                                                                                    <  2:122248/140‑1 (MQ=255)
                                                taaTATCTGTATTTTTTTATTTTGGAATTTCGATTTCGGGTTCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTa                                                                                   <  1:327879/139‑1 (MQ=255)
                                                 aaTATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACAGATGATCATTTTTTGATTGAGGTAAAAATGGTTCTACTATACTTTCTCGTTTAt                                                                                  >  2:516366/1‑140 (MQ=255)
                                                  atatCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTAta                                                                                 >  2:547835/1‑139 (MQ=255)
                                                  atatCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGATGTAACAATTTCATACACATGATCATTATTTGATTGAGATAAAAATGATTCTACTAAACGTACACGTTTATg                                                                                 >  1:123169/1‑140 (MQ=255)
                                                   tatCGGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGc                                                                                <  2:597904/140‑1 (MQ=255)
                                                    atCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATCATACTACTAAACGTACACGTTTATGct                                                                               >  2:49030/1‑140 (MQ=255)
                                                      cTGTTTTTTTATATTTTGGAATTTTGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTGCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGcttc                                                                             <  1:480400/140‑1 (MQ=255)
                                                        gTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTCAACGTTTATGcttctt                                                                           >  1:634856/1‑140 (MQ=255)
                                                        gTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGcttctt                                                                           <  1:550895/140‑1 (MQ=255)
                                                         tATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTta                                                                          >  2:134423/1‑140 (MQ=255)
                                                         tATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTta                                                                          >  2:463212/1‑140 (MQ=255)
                                                          agttttGTATTTTGGAATTTCGATTTCGGGTTCTCGCTATTATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTtat                                                                         <  2:637214/138‑1 (MQ=255)
                                                          aTTTTTATATTTTTGAATTTCGATTTCGGGTCATCAAAATCATAATTTTTTATAAATTTGTTTAACAATTTCATACACATTATCATTCTTTGATTGAGGTAAAAATGATTCTACTCAACGTACACGTTTACGCTTCTtat                                                                         <  2:636774/140‑1 (MQ=255)
                                                          aTTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTtat                                                                         <  2:192108/140‑1 (MQ=255)
                                                           tttttATATTTTGGAATTTCGATTTCGGGTTCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTtata                                                                        <  1:161024/140‑1 (MQ=255)
                                                              ttATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTc                                                                     <  1:226357/140‑1 (MQ=255)
                                                                 tatTTTGGAATTTCGATTTCGGTTCCTCGATAATATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCttt                                                                  <  2:360155/140‑1 (MQ=255)
                                                                 tatTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTACAGATGAGGTGGCTAAACGTACACGTTTATGCTTCTTATCTTCttt                                                                  >  1:451474/1‑140 (MQ=255)
                                                                 tatTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCttt                                                                  >  2:99037/1‑140 (MQ=255)
                                                                    tttGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTtcat                                                               >  2:425008/1‑140 (MQ=255)
                                                                         aaTTTCGATTTCGGGTCCTAGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCaata                                                          <  1:577876/140‑1 (MQ=255)
                                                                           tttcgatttcgGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCaataaa                                                        <  1:134423/140‑1 (MQ=255)
                                                                           tttcgatttcgGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCaataaa                                                        <  1:463212/140‑1 (MQ=255)
                                                                              cgatttcgGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAatat                                                     >  2:380028/1‑140 (MQ=255)
                                                                              cgatttcgGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAatat                                                     >  1:334635/1‑140 (MQ=255)
                                                                               gatttcgGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATATACATGATTATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATc                                                    <  1:130908/140‑1 (MQ=255)
                                                                                atttcgGGTCCTCGATAAGATGATTTTTTGTAAATTGGTGTGACAATTTCATACACATGATCATTCTTTGATTGAGGTAATAATGATTATACTAAACGTAAACGTTTATGCTTTTTATATTATTTTATCAATAAatatgt                                                   >  1:602070/1‑138 (MQ=255)
                                                                                        tCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAg                                           >  1:209979/1‑140 (MQ=255)
                                                                                           tCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTa                                        >  2:604406/1‑140 (MQ=255)
                                                                                            cGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTaa                                       <  2:223412/140‑1 (MQ=255)
                                                                                            cGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTATACTAAACGTACACGTTTATGCTTCTTATATTATTTCATCAATAAATATTTTTGATTAGCTaa                                       >  1:603238/1‑140 (MQ=255)
                                                                                               tAAGTTGATTTTTTACAAATTGGTGTAACAATTTCGTACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGa                                    <  2:339893/140‑1 (MQ=255)
                                                                                               tAAGATGATTTTTTATAAATTGGTGTAACAATTTCGTACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGa                                    <  2:528943/140‑1 (MQ=255)
                                                                                               tAAGATGATTTTTTATAAATTGGTGTAACAATTTCGTACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGa                                    <  1:576640/140‑1 (MQ=255)
                                                                                               tAAGAGTATTTTTTATAAATTAGTTTAATAACTTCGTACACATGATCATTTTTTGATTGAGGTAAAAATTATTCTACTAAACGTACACGTTTATGCTTCTTATATACTTTAATCAATAAATATCTATGATTAGCTAATGa                                    <  1:136639/140‑1 (MQ=255)
                                                                                                aaGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGAt                                   >  1:86776/1‑140 (MQ=255)
                                                                                                 aGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATa                                  <  2:25891/140‑1 (MQ=255)
                                                                                                 aGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATa                                  <  2:238983/140‑1 (MQ=255)
                                                                                                    ttatTTTTTATAACTTGTTGTAACAATTTCATACACATGATCATTCTTTGATTGAAGTAAAAATCATCCTACCAAACGTGCACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATAAAt                               <  2:402539/138‑1 (MQ=255)
                                                                                                        tttttATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATAAATTtag                           <  2:209979/140‑1 (MQ=255)
                                                                                                        tttttATAAATTGGTGGAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTAAGATTTGCGGATGATAAATTtag                           <  2:307500/140‑1 (MQ=255)
                                                                                                          ttttgaaaTTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGGGGTAAAAATGATTCTACTAAACGTACGCGTTTATGCTTCTTATATTCTTTTATCAATAAATATCTATGATTAGCTAATGATAAATTtagta                         <  2:202361/135‑1 (MQ=255)
                                                                                                          tttATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATAAATTtagta                         <  1:217362/140‑1 (MQ=255)
                                                                                                          tttATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATAAATTtagta                         <  1:202061/140‑1 (MQ=255)
                                                                                                           ttATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTTGCTAATGATAAATTtagtag                        >  1:149967/1‑140 (MQ=255)
                                                                                                              taAATTTGTGTAACAACATCATACAGGTGGTCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCGATAAATATCTATGATTAGCTAATGATAAATTTAGTAGAAg                     <  2:634856/140‑1 (MQ=255)
                                                                                                                aaTTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATAAATTTAGTAGAAGCt                   <  1:99037/140‑1 (MQ=255)
                                                                                                                     gtgtAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATAAATTTAGTAGAAGCTTGTTa              <  1:628665/140‑1 (MQ=255)
                                                                                                                              tttCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATAAATTTAGTAGAAGCTTGTTATCTCCATaa     >  1:613510/1‑140 (MQ=255)
                                                                                                                                 cATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATAAATTTATTAGAAGCTTGTTATCTCCATAAACt  >  1:103295/1‑140 (MQ=255)
                                                                                                                                  |                                                                                                                                          
ATGGCTTATCGATATGAATATGTAATGCAAACCAGTCTTTGTCATATTCAATATCTGTATTTTTATATTTTGGAATTTCGATTTCGGGTCCTCGATAAGATGATTTTTTATAAATTGGTGTAACAATTTCATACACATGATCATTCTTTGATTGAGGTAAAAATGATTCTACTAAACGTACACGTTTATGCTTCTTATATTCTTTCATCAATAAATATCTATGATTAGCTAATGATAAATTTAGTAGAAGCTTGTTATCTCCATAAACT  >  USA300TCH1516_ALE/1952509‑1952777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: