Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	728,366	G→A	6.6%	G79S (GGT→AGT)	USA300HOU_RS03535 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	728,366	0	G	A	6.6%	185.6 / 3.2	68	G79S (GGT→AGT)	USA300HOU_RS03535	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (35/28);  new base A (3/2);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

TTCACTAAATATGGCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCTAGGTGCTTTAATTATGA  >  USA300TCH1516_ALE/728228‑728504                                                                                                                                           |                                                                                                                                           ttCACTAAATAGGACTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTGAGCTTTTATTGGGCGTCAAGTACCAAAAATTGGTACTGCCTTTGAAGAGACAAATCATGAATCAACATTACAATACGTATTCgg                                                                                                                                           <  2:186084/140‑1 (MQ=255)  tCACTTAATATGGCTTGTAGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGt                                                                                                                                          <  2:635271/140‑1 (MQ=255)   cACTAAATATGGCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTg                                                                                                                                         <  2:256057/140‑1 (MQ=255)    aCTAAATATGGCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGATTCAACATTACAATACGTATTCGTTGa                                                                                                                                        >  1:649942/1‑140 (MQ=255)     cTAAATATGGCTTGTGGGCTTATTTAGGTGATATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTGCAATACGTATTCGGTGaa                                                                                                                                       >  2:572347/1‑140 (MQ=255)             ggCTTGTGGGCTTATTTAGGTGTTGTTATATCTGGTTTTATTTTATCTTTTATTGGACGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAg                                                                                                                               >  1:342906/1‑140 (MQ=255)              ttttGTGGGTTTATTTATGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCACGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGt                                                                                                                              <  1:164917/138‑1 (MQ=255)              gCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGt                                                                                                                              <  2:95041/140‑1 (MQ=255)              gCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGt                                                                                                                              >  2:533809/1‑140 (MQ=255)               cTTGTGGGCTTATTTGGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTa                                                                                                                             >  1:494694/1‑140 (MQ=255)               cTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTa                                                                                                                             >  1:662980/1‑140 (MQ=255)               cTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTa                                                                                                                             >  1:266259/1‑140 (MQ=255)               cTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTa                                                                                                                             >  1:93418/1‑140 (MQ=255)               cTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGAGCGACAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTa                                                                                                                             >  1:21491/1‑140 (MQ=255)                 tgtgGGCTTATTTATGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTaaa                                                                                                                           <  2:446221/140‑1 (MQ=255)                     ggCTTATTTAGTTGTTATTATATCTTGTTTTATTTTAGCTTTTATTGGGCGGCAAGTAGCAAAAATTGGTACTGCTTTTGAAGCGACAAATCATGAATCAACCTTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCt                                                                                                                       <  2:30992/140‑1 (MQ=255)                      gCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGACGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCtt                                                                                                                      >  2:427161/1‑140 (MQ=255)                       cTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCttt                                                                                                                     >  1:388789/1‑140 (MQ=255)                          aTTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGAt                                                                                                                  <  2:483911/140‑1 (MQ=255)                                        atatCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAAtctt                                                                                                    >  1:640956/1‑140 (MQ=255)                                        atatCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATAATGAATCAACATTACAATACGTATGCGGGTAAAAGTTTAGTAAAGTCGTTGATTATATTTTATtctt                                                                                                    >  1:177862/1‑140 (MQ=255)                                         tatCTTGTTTTATTTTAGCTTTTATTGGGTGCCAAGTAGCATAAATTGGTACTGGCTTTGAATCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAAtcttc                                                                                                   <  1:143511/140‑1 (MQ=255)                                          atCTTGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAAtcttct                                                                                                  <  1:225988/140‑1 (MQ=255)                                          atCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTTGCTTTTATTGGTTCTTTTTTTGTTTCGACTTGTCTACAAACAATTTTTTGAATCTAATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAAtcttct                                                                                                  <  1:319642/140‑1 (MQ=255)                                          atCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAAtcttct                                                                                                  >  2:542703/1‑140 (MQ=255)                                               gTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGCATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGACTTTGATTATATTTTAATcttcttcttc                                                                                             >  2:578842/1‑139 (MQ=255)                                                ttttattttaGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTAt                                                                                            <  1:99991/140‑1 (MQ=255)                                                 tttattttaGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTATAGTAAAGTCTTTGATTATATTTTAATCTTCTCCTTAtt                                                                                           >  2:339465/1‑140 (MQ=255)                                                    attttaGCTTTTATTGGGCGCAAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTTTTCTTATTTgt                                                                                        >  2:438936/1‑139 (MQ=255)                                                        taGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGtata                                                                                    >  1:339112/1‑140 (MQ=255)                                                         aGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAg                                                                                   >  1:44617/1‑140 (MQ=255)                                                         aGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGAGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAg                                                                                   >  2:561743/1‑140 (MQ=255)                                                                     gcgcCAAGTAGCAAAAATTGGTACTCCCTTTTAAGTGACAAACCATGACTCAATATTACAATACGTATCCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGa                                                                       <  2:192917/140‑1 (MQ=255)                                                                           aGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTCGATTATATTTTAATTTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTg                                                                 >  1:548445/1‑140 (MQ=255)                                                                            gTAGCAAAAATTGGTATTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTgg                                                                <  2:582498/140‑1 (MQ=255)                                                                             tAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAATTTAGAAAAATCTTTGATTATATTTTAATCTTCTTCTTATTTCGAAGAGCTTGAACCATGATAGCTGGt                                                               >  1:152212/1‑140 (MQ=255)                                                                             tAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCAGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGt                                                               >  2:231650/1‑140 (MQ=255)                                                                             tAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCAGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGt                                                               >  2:231678/1‑140 (MQ=255)                                                                             tAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCAGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGt                                                               >  1:207526/1‑140 (MQ=255)                                                                              aGCAAAAATTGGTACTGTCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTTATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTg                                                              <  2:248739/140‑1 (MQ=255)                                                                                 aaaaaTTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAg                                                           >  2:61672/1‑140 (MQ=255)                                                                                     aTTGTTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGgcgc                                                       <  2:122203/140‑1 (MQ=255)                                                                                      ttGGTACTGCCTTTGAATCGACAAATCATGAATCAACATTACAAAACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCa                                                      <  2:69803/140‑1 (MQ=255)                                                                                      ttGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTACTCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCa                                                      <  2:2647/140‑1 (MQ=255)                                                                                      ttGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCa                                                      <  1:420877/140‑1 (MQ=255)                                                                                        ggTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGATTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAACTGGTGCAGGCGCAAc                                                    >  2:428650/1‑140 (MQ=255)                                                                                         gTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACa                                                   >  1:410378/1‑140 (MQ=255)                                                                                         gTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACa                                                   >  2:552124/1‑140 (MQ=255)                                                                                          tACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACAt                                                  <  1:533809/140‑1 (MQ=255)                                                                                          tACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACAt                                                  <  1:610175/140‑1 (MQ=255)                                                                                           acagCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACAtt                                                 >  2:243918/4‑140 (MQ=255)                                                                                           aCTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACAtt                                                 >  1:498097/1‑140 (MQ=255)                                                                                             tGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTg                                               <  2:44617/140‑1 (MQ=255)                                                                                               ccTTTGAAGCGACAAATCATGAATCAACATTGCAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTgaa                                             >  2:330185/1‑140 (MQ=255)                                                                                                 tttGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTgaaga                                           <  2:172267/140‑1 (MQ=255)                                                                                                      aGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGtt                                      >  1:247267/1‑140 (MQ=255)                                                                                                          aCAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCATCATCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGCAGAAAGTTATaa                                  >  1:243973/1‑140 (MQ=255)                                                                                                               tCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTCTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAATTTATAACATAc                             <  2:172734/140‑1 (MQ=255)                                                                                                                  tGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTa                          >  1:374740/1‑140 (MQ=255)                                                                                                                   gAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTag                         >  2:326557/1‑139 (MQ=255)                                                                                                                        aaCATTACAATACGTATTCGGTTAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGCTATAGCTGTAACCATCATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGc                    <  2:342906/140‑1 (MQ=255)                                                                                                                         aCATTACAATACGTATTCAGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCt                   <  1:231650/140‑1 (MQ=255)                                                                                                                         aCATTACAATACGTATTCAGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCt                   <  1:231678/140‑1 (MQ=255)                                                                                                                          cactACAATACGTACTAGGTGAAAGGTATAGTAAAGTCCTTGATTATATTTTACTCTTATTCTTCTTTGGTATAGATGTAGCCATGATCGCTGGTGTAGATGCAACAATTGAGAAAAGTTATAACATACCTACCTGGCTa                  >  2:186665/4‑140 (MQ=255)                                                                                                                                  tACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGTAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCTAGGTGCttt          <  2:649942/140‑1 (MQ=255)                                                                                                                                      tATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACTTACCTACATGGCTAGGTGCTTTAAtt      <  2:592165/140‑1 (MQ=255)                                                                                                                                       aTTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGTAACATTTGAAGAAAGTTATAACATACCTAGATGGCTGGGAGCTTTAATTa     >  2:7875/1‑140 (MQ=255)                                                                                                                                         tCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCTAGGTGCTTTAATTATg   <  2:388789/140‑1 (MQ=255)                                                                                                                                          cGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCTAGGTGCTTTAATTATGa  <  1:81618/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                           TTCACTAAATATGGCTTGTGGGCTTATTTAGGTGTTATTATATCTGGTTTTATTTTAGCTTTTATTGGGCGCCAAGTAGCAAAAATTGGTACTGCCTTTGAAGCGACAAATCATGAATCAACATTACAATACGTATTCGGTGAAAAGTTTAGTAAAGTCTTTGATTATATTTTAATCTTCTTCTTATTTGGTATAGCTGTAACCATGATAGCTGGTGCAGGCGCAACATTTGAAGAAAGTTATAACATACCTACATGGCTAGGTGCTTTAATTATGA  >  USA300TCH1516_ALE/728228‑728504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑