Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,125,066 G→C 100% E729Q (GAG→CAG)  cfiB_1 → 2‑oxoglutarate carboxylase small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,125,0660GC100.0% 100.6 / NA 29E729Q (GAG→CAG) cfiB_12‑oxoglutarate carboxylase small subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (16/13);  total (16/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATCATTGATACTGCTGTTGCTTCAATGAGTGGTT  >  USA300TCH1516_ALE/1124936‑1125201
                                                                                                                                  |                                                                                                                                       
aTCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATc                                                                                                                               <  1:63643/141‑1 (MQ=255)
aTCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATAAGGCAGGCTTATTAAAACCTAAAGCCGCTTACGACATGATTGGTCAGTTAAAATc                                                                                                                               <  1:256697/141‑1 (MQ=255)
 tCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCa                                                                                                                              >  2:382944/1‑141 (MQ=255)
 tCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCa                                                                                                                              >  2:348981/1‑141 (MQ=255)
   aaaCATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAACCGCTTACGAATTGATTGGTCAGTTAAAAACAGc                                                                                                                            >  2:237448/1‑141 (MQ=255)
           ataCTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGAtt                                                                                                                    <  1:111313/141‑1 (MQ=255)
              cTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGTACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTAc                                                                                                                 >  2:65611/1‑141 (MQ=255)
                   gggTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATCGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAAtt                                                                                                            <  1:380331/139‑1 (MQ=255)
                                       tAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCTTGGTAc                                                                                        <  2:46670/141‑1 (MQ=255)
                                       tAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATAc                                                                                        <  1:357351/141‑1 (MQ=255)
                                           gagTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGt                                                                                    >  1:143540/1‑141 (MQ=255)
                                                 gAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAAt                                                                              >  1:151445/1‑141 (MQ=255)
                                                 gAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAAt                                                                              >  1:200003/1‑141 (MQ=255)
                                                  aaCGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATg                                                                             >  1:279497/1‑141 (MQ=255)
                                                          ggTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGGAATGGTttatta                                                                     >  1:328090/1‑141 (MQ=255)
                                                           gataccATATTTTAGCGATTAAAGATATGGCACGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAACCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTaa                                                                    <  1:72305/137‑1 (MQ=255)
                                                                  tatTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATacaa                                                             <  1:391565/141‑1 (MQ=255)
                                                                  aatTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATacaa                                                             <  1:391501/140‑1 (MQ=255)
                                                                    ttttAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTAATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACAGTCATGATACAAGTGGGAATGGTTTATTAACATacaaaa                                                           >  1:116536/1‑140 (MQ=255)
                                                                      ttAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCATCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATacaaacaa                                                         <  1:269930/141‑1 (MQ=255)
                                                                                aagtacgggCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGCTTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATg                                               <  1:27917/134‑1 (MQ=255)
                                                                                aaGATATGGCAGGCTTGTTAATACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATCGAtt                                               >  1:203373/1‑140 (MQ=255)
                                                                                aaGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATg                                               >  1:237399/1‑141 (MQ=255)
                                                                                              ttattaAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATc                                 >  1:74196/1‑141 (MQ=255)
                                                                                                     aaCCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATCATTGATa                          >  1:326328/1‑141 (MQ=255)
                                                                                                       ccTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAAAACGAAGAACATGCTGGTGTCGATATCATTGATAct                        <  1:191386/141‑1 (MQ=255)
                                                                                                            aGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATCATTGATACTGCTGt                   <  2:388938/141‑1 (MQ=255)
                                                                                                                    aCGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGGGTCGATATCATTGATACTGCTGTTGCTTCaa           >  1:312496/1‑141 (MQ=255)
                                                                                                                             ttgGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATCATTGATACTGCTGTTGCTTCAATGTGTGGtt  >  2:27625/1‑141 (MQ=255)
                                                                                                                                  |                                                                                                                                       
ATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATCATTGATACTGCTGTTGCTTCAATGAGTGGTT  >  USA300TCH1516_ALE/1124936‑1125201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑