| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,830,883 | C→T | 75.3% | K79K (AAG→AAA) | ezrA ← | Septation ring formation regulator EzrA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,830,883 | 0 | C | T | 75.3% | 43.8 / 10.4 | 28 | K79K (AAG→AAA) | ezrA | Septation ring formation regulator EzrA |
| Reads supporting (aligned to +/- strand): ref base C (4/3); new base T (10/11); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCTTTGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGAT > USA300TCH1516_ALE/1830759‑1831017 | aGCTTTGTTCGTAACTGTCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTa > 1:234804/1‑141 (MQ=255) gCTTTGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTCTCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCCCAGGAGCTAGATATTTATTTGTACTTTCtcc > 2:118534/1‑139 (MQ=255) tttGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGt > 2:274986/1‑141 (MQ=255) tGTTCGTAACTATACATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTg < 1:324198/141‑1 (MQ=255) cGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCt > 1:18274/1‑141 (MQ=255) aaCTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCtttt > 1:135463/1‑141 (MQ=255) tATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTGATGTGGACTTTCTACGTTGTCCTTTTTCa < 1:191391/141‑1 (MQ=255) ccgcttttCATCATCAATTTCACATTCAGATGCGTTGAAACTAAAGTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGCTGCTAGATACTTATTTGTACCTTCTACGTTGTCCTTTTTCATTGCATCGTa < 2:29543/137‑1 (MQ=255) aTTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTAttt > 1:333305/1‑141 (MQ=255) gCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGtt > 2:23443/1‑141 (MQ=255) aTTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATTAAGCTTCAGCATTATGTATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCa < 2:65901/141‑1 (MQ=255) cTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTACTGTTCCAAAGGAGGTAGAGATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCtttt > 1:189776/1‑141 (MQ=255) tttttgaTGCTTTGAATCTAAATTTATTTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTa < 1:113731/136‑1 (MQ=255) tttGAGATGCGTTGAAACTAAACTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTa < 1:43913/141‑1 (MQ=255) ttGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTGCACAGGAGCTAGATCTTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTTTCCTTTGAAGTTCAACTTa > 1:272638/1‑141 (MQ=255) gAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGa < 1:180167/141‑1 (MQ=255) gAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGa < 1:116661/141‑1 (MQ=255) ttATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCa < 2:307426/141‑1 (MQ=255) ataaAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGtttt > 2:123196/1‑141 (MQ=255) aGCCTCAGTATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATc < 1:23443/141‑1 (MQ=255) cTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCaaa > 1:360174/1‑141 (MQ=255) tCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCaaaa > 2:56411/1‑141 (MQ=255) cAGCATTATTGATTTTTTCGGCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAg < 1:130302/141‑1 (MQ=255) cATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTa > 1:256056/1‑141 (MQ=255) gAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGtt < 1:92144/141‑1 (MQ=255) gAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGtt < 1:351326/141‑1 (MQ=255) gAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGtt < 1:341104/141‑1 (MQ=255) tAGATATTTATTTGTACTTTCTCCTTTGGCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGAt > 1:291154/1‑141 (MQ=255) | AGCTTTGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGAT > USA300TCH1516_ALE/1830759‑1831017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |