Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	536,758	C→T	P202L (CCA→CTA)	rplY →	50S ribosomal protein L25

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	536,758	0	C	T	100.0%	207.6 / NA	62	P202L (CCA→CTA)	rplY	50S ribosomal protein L25
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/43);  total (19/43)

GTTGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGTTATTATAAAGCT  >  USA300TCH1516_ALE/536619‑536902                                                                                                                                            |                                                                                                                                                 gTTGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAgttg                                                                                                                                          >  4:294662/1‑148 (MQ=255)      tGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGc                                                                                                                                     <  4:376559/148‑1 (MQ=255)          gTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGaaa                                                                                                                                 >  3:334787/1‑148 (MQ=255)          gTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGaaa                                                                                                                                 <  3:160803/148‑1 (MQ=255)               aGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCaaa                                                                                                                            >  4:154196/1‑148 (MQ=255)                      ggCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGTTCCTACTGAAGAAGCAACCGCAGCGATGGACGCCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACg                                                                                                                     <  4:340086/148‑1 (MQ=255)                        cGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACgaa                                                                                                                   <  3:54090/148‑1 (MQ=255)                        cGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACgaa                                                                                                                   <  3:413303/148‑1 (MQ=255)                             tCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGaaaa                                                                                                              <  3:349881/148‑1 (MQ=255)                             tCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGaaaa                                                                                                              <  3:348296/148‑1 (MQ=255)                              cAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACGGAAGAAGAAAGCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTGGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGaaaaa                                                                                                             >  3:208366/1‑148 (MQ=255)                                        aaCGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGt                                                                                                   >  4:109217/1‑148 (MQ=255)                                        aaCGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAAATGAAGAGt                                                                                                   >  4:379989/1‑148 (MQ=255)                                              tCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAagag                                                                                                    <  1:39334/141‑1 (MQ=255)                                              tCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAAtttt                                                                                             <  3:308548/148‑1 (MQ=255)                                                 gCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAAt                                                                                          >  4:36615/1‑148 (MQ=255)                                                 gCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAAt                                                                                          >  4:273868/1‑148 (MQ=255)                                                    gAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTg                                                                                       <  4:77402/148‑1 (MQ=255)                                                     aaTCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGt                                                                                      <  3:6082/148‑1 (MQ=255)                                                        cAGGAGTAACAGTAGTTGCTCCAACTGCAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAAt                                                                                          <  2:39186/141‑1 (MQ=255)                                                           tagtaACAGTAGTTGCTCCAACTGAAGAACCAACTG‑AGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTa                                                                               <  3:102342/148‑1 (MQ=255)                                                            agtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGATGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGt                                                                                      <  1:53546/141‑1 (MQ=255)                                                            agtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTa                                                                               <  3:278338/148‑1 (MQ=255)                                                            agtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTa                                                                               <  3:201158/148‑1 (MQ=255)                                                            agtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTa                                                                               <  3:99175/148‑1 (MQ=255)                                                             gtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTaa                                                                              >  3:406993/1‑148 (MQ=255)                                                                 cAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGtt                                                                          <  3:238645/148‑1 (MQ=255)                                                                 cAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGtt                                                                          <  3:83366/148‑1 (MQ=255)                                                                 cAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGtt                                                                          <  3:349943/148‑1 (MQ=255)                                                                 aagtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACAt                                                                                 <  2:47878/140‑1 (MQ=255)                                                                  agtagtTGTTGCAACTGAAGAAACAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAAAAGAAAAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         <  4:411383/148‑1 (MQ=255)                                                                  agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAGGttt                                                                         <  3:326414/148‑1 (MQ=255)                                                                  agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         >  3:99358/1‑148 (MQ=255)                                                                  agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         <  4:276704/148‑1 (MQ=255)                                                                  agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         <  3:401769/148‑1 (MQ=255)                                                                  agtagtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         >  3:275660/1‑148 (MQ=255)                                                                  agtagtTACTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                         <  4:114143/148‑1 (MQ=255)                                                                      gtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAGAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTtat                                                                     <  4:85790/148‑1 (MQ=255)                                                                        tGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATAc                                                                   >  3:100017/1‑148 (MQ=255)                                                                           tCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACttt                                                                <  3:163931/148‑1 (MQ=255)                                                                            ccaactgaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTta                                                                      <  2:5000/141‑1 (MQ=255)                                                                            ccaactgaagaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTg                                                               >  4:215008/1‑148 (MQ=255)                                                                                           actgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACtgt                                                <  3:134033/148‑1 (MQ=255)                                                                                           actgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACtgt                                                <  3:249206/148‑1 (MQ=255)                                                                                           actgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAAAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGACGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACaa                                                       <  1:53552/141‑1 (MQ=255)                                                                                             tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAATAAAGATCTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGc                                              <  4:369391/148‑1 (MQ=255)                                                                                             tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGc                                                     >  1:27067/1‑141 (MQ=255)                                                                                             tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGc                                              <  3:277488/148‑1 (MQ=255)                                                                                             tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGc                                              <  4:229269/148‑1 (MQ=255)                                                                                             tgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGc                                              >  4:237341/1‑148 (MQ=255)                                                                                               aagaagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAAAAAAAAAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCtt                                            <  4:95386/148‑1 (MQ=255)                                                                                                     gaaggtgaGCTATGGGAGGCGAAGAGGAAGCTGGAGGACTGGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCt                                             <  2:17043/134‑1 (MQ=255)                                                                                                     aaaTCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTa                                      <  3:27922/148‑1 (MQ=255)                                                                                                                  ggAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGt                         <  3:377331/148‑1 (MQ=255)                                                                                                                  ggAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGt                         >  3:331461/1‑148 (MQ=255)                                                                                                                  ggAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGt                         <  3:294662/148‑1 (MQ=255)                                                                                                                           acaacaAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATGTAAGCATGGTGCTTTTtgt                <  3:273868/148‑1 (MQ=255)                                                                                                                           acaacaAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTtgt                <  3:36615/148‑1 (MQ=255)                                                                                                                                  aCTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGttatta         >  4:136863/1‑148 (MQ=255)                                                                                                                                  aCTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGttatta         >  4:373918/1‑148 (MQ=255)                                                                                                                                  aCTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTATTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGttatta         >  4:344233/1‑148 (MQ=255)                                                                                                                                         aaCTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGTTATTATAAAGCt  <  4:148176/148‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                 GTTGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGTTATTATAAAGCT  >  USA300TCH1516_ALE/536619‑536902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑