Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	744,375	A→T	I506F (ATT→TTT)	melR_1 →	Melibiose operon regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	744,375	0	A	T	100.0%	160.6 / NA	46	I506F (ATT→TTT)	melR_1	Melibiose operon regulatory protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (19/27);  total (19/27)

AATAAAGCGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATATTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAA  >  USA300TCH1516_ALE/744233‑744516                                                                                                                                               |                                                                                                                                              aaTAAAGCGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGaa                                                                                                                                          <  3:14269/148‑1 (MQ=255)       gCGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGaa                                                                                                                                    >  3:104416/1‑148 (MQ=255)       gCGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGaa                                                                                                                                    >  3:304577/1‑148 (MQ=255)        cGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGaaa                                                                                                                                   <  4:142390/148‑1 (MQ=255)        cGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGaaa                                                                                                                                   <  4:140497/148‑1 (MQ=255)            ttAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCt                                                                                                                               <  3:66073/148‑1 (MQ=255)            ttAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCt                                                                                                                               <  3:161419/148‑1 (MQ=255)                    gattcgTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTc                                                                                                                       <  3:19445/148‑1 (MQ=255)                           taataaTAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGt                                                                                                                >  3:359652/1‑148 (MQ=255)                            aataatAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAATCAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGtt                                                                                                               <  3:248433/148‑1 (MQ=255)                                  aGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAAt                                                                                                         <  4:80445/148‑1 (MQ=255)                                  aGATAATGCAAATTTAAGTAGACATTCTTTGGAAAGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAAt                                                                                                         >  4:415789/1‑148 (MQ=255)                                    aTAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCa                                                                                                       >  4:340152/1‑148 (MQ=255)                                     tAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCAt                                                                                                      >  3:250023/1‑148 (MQ=255)                                      aaTGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATa                                                                                                     <  4:385196/148‑1 (MQ=255)                                       aTGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGaaa                                                                                                           >  2:6558/1‑141 (MQ=255)                                          cAAATTTAAGTAGCCCTTATTTGATGGGGTAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACt                                                                                                 <  4:217985/148‑1 (MQ=255)                                           aaaTTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCa                                                                                                       <  1:2216/141‑1 (MQ=255)                                           aaaTTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTg                                                                                                <  3:327056/148‑1 (MQ=255)                                             aTTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCt                                                                                              <  4:380436/148‑1 (MQ=255)                                             aTTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCt                                                                                              <  4:51690/148‑1 (MQ=255)                                                 aaGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTtaat                                                                                          <  4:107761/148‑1 (MQ=255)                                                   gTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTtaataa                                                                                        <  3:59877/148‑1 (MQ=255)                                                           tATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAAc                                                                                >  3:338150/1‑148 (MQ=255)                                                           tATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAAc                                                                                >  4:63754/1‑148 (MQ=255)                                                                    ggggAAAGTGATGAGTTACTATTCAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACga                                                                              <  1:57918/141‑1 (MQ=255)                                                                             gatgaGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGTTGATTTTTCTAAATGTTGAAAATCATAAACTGATTAATAATAAAGAACGAGATCTaatt                                                                     >  1:40157/1‑139 (MQ=255)                                                                                  gTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTACATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCa                                                         <  4:160304/148‑1 (MQ=255)                                                                                   ttACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCAt                                                        <  3:116413/148‑1 (MQ=255)                                                                                   ttACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCAt                                                        <  3:287794/148‑1 (MQ=255)                                                                                          tGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCAt                                                        >  2:58065/1‑141 (MQ=255)                                                                                             aaaaTATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATaa                                              >  4:321959/1‑148 (MQ=255)                                                                                              aaaTATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAg                                             <  4:315656/148‑1 (MQ=255)                                                                                                atatTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGAc                                           >  3:16460/1‑148 (MQ=255)                                                                                                atatTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGAc                                           >  3:303323/1‑148 (MQ=255)                                                                                                        cATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTGAATAATAAAGAACGAGATCTAGGTAATAGTGCTCCATTAATTTATAAGACATTAAGTg                                   >  3:144768/1‑148 (MQ=255)                                                                                                          ttttAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTgcg                                 >  3:389442/1‑148 (MQ=255)                                                                                                               aaaaTTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTa                            >  3:259525/1‑148 (MQ=255)                                                                                                                 aaTTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATc                          <  3:415789/148‑1 (MQ=255)                                                                                                                      aaaaCAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAAc                     <  4:105026/148‑1 (MQ=255)                                                                                                                      aaaaCAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAAc                     <  3:332514/148‑1 (MQ=255)                                                                                                                             gTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATg              <  3:2379/148‑1 (MQ=255)                                                                                                                                attaatGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCt           <  3:370191/148‑1 (MQ=255)                                                                                                                                    atGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTgg       <  4:238259/148‑1 (MQ=255)                                                                                                                                     tGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGa      >  4:353012/1‑148 (MQ=255)                                                                                                                                         ttGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTaa  >  3:137787/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                              AATAAAGCGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATATTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAA  >  USA300TCH1516_ALE/744233‑744516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑