Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,017,678	T→C	F391S (TTC→TCC)	acp →	Sodium/proton‑dependent alanine carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,017,678	0	T	C	100.0%	116.2 / NA	31	F391S (TTC→TCC)	acp	Sodium/proton‑dependent alanine carrier protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/15);  total (16/15)

AAATGCAACAGGTAAAGATTATTCAGGTACTGCGATGTATGCACAAGCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTTCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCAAAATAATCAAGTTTCATCGATATTTATTAATATTGCTCGTGTGATTATTTTGTTCGCTACA  >  USA300TCH1516_ALE/1017538‑1017811                                                                                                                                             |                                                                                                                                      aaaTGCAACAGGTAAAGATTATTCAGGTACTGCGATGTATGCACAAGCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCa                                                                                                                                >  3:112717/1‑148 (MQ=255) aaaTGCAACAGGTAAAGATTATTCAGGTACTGCGATGTATGCACAAGCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCa                                                                                                                                >  3:78962/1‑148 (MQ=255)       aaCAGGTAAAGATTATTCAGGTACTGCGATGTATGCACAAGCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGTTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTtact                                                                                                                          <  3:116950/148‑1 (MQ=255)       aaCAGGTAAAGATTATTCAGGTACTGCGATGTATGCACAAGCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTtact                                                                                                                          <  3:371404/148‑1 (MQ=255)        aCAGGTAAAGATTATTCAGGTACTGCGATGTATGCACAAGCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCa                                                                                                                                >  1:36357/1‑141 (MQ=255)                  attattCAGGTACTGCGATGTATGCACAAGCCGGCATTGATAAGGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTc                                                                                                               >  4:368441/1‑148 (MQ=255)                    tattCAGTTACTGCGATGTATGCACAAGCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGt                                                                                                             <  4:82216/148‑1 (MQ=255)                    tattCAGGTACTGCGATGTATGCACAAGCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGt                                                                                                             <  4:43606/148‑1 (MQ=255)                       tCAGGTACTGCGATGTATGCACAAGCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGtact                                                                                                          <  4:326974/148‑1 (MQ=255)                          ggTACTGCGATGTATGCACAAGCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGtactact                                                                                                       >  4:194168/1‑148 (MQ=255)                                               gCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTg                                                                                  <  3:338774/148‑1 (MQ=255)                                                   gCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTa                                                                              <  3:235347/148‑1 (MQ=255)                                                            aaGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAAcgcg                                                                     >  4:106475/1‑148 (MQ=255)                                                                 ttCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATc                                                                <  3:194168/148‑1 (MQ=255)                                                                   ccATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAATTGTTGTTTTTTTAACGCGTAATCaa                                                              >  3:109237/1‑148 (MQ=255)                                                                   ccATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCaa                                                              >  4:338805/1‑148 (MQ=255)                                                                   ccATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCaa                                                              >  4:76004/1‑148 (MQ=255)                                                                   ccATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCaa                                                              >  4:52174/1‑148 (MQ=255)                                                                   ccATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCaa                                                              >  4:355837/1‑148 (MQ=255)                                                                   ccATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCaa                                                              >  3:126622/1‑148 (MQ=255)                                                                   ccATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCaa                                                              >  4:230222/1‑148 (MQ=255)                                                                      tGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCAAaat                                                           >  4:403774/1‑148 (MQ=255)                                                                       ggCCGTGGTTTTCAATTTGATCCTACTTTCCCCGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAAt                                                                 <  2:24237/141‑1 (MQ=255)                                                                       ggCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAAt                                                                 <  2:17646/141‑1 (MQ=255)                                                                       ggCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCAAaata                                                          <  3:181242/148‑1 (MQ=255)                                                                                           tCCTTCTNTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACTGAAACAAATGTTGCTTATTTAACGCGTAATCAAAATAATCAAGTATCATCGAtattt                                      <  4:312034/148‑1 (MQ=255)                                                                                             cTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCAAAATAATCAAGTTTCATCGAtatttat                                    >  4:196631/1‑148 (MQ=255)                                                                                                              ggTTCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCAAAATAATCAAGTTTCATCGATATTTATTAATATTGCTCGTGTGa                   <  3:84679/148‑1 (MQ=255)                                                                                                                 tCGTACTTTATTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCAAAATAATCAAGTTTCATCGATATTTATTAATATTGCTCGTGTGatta                <  4:57123/148‑1 (MQ=255)                                                                                                                 tCGTACTTTAGTGCATTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCAAAATAATCAAGTTTCATCGATATTTATTAATATTGCTCGTGTGatta                <  4:50240/148‑1 (MQ=255)                                                                                                                               aTTTGCTTTATTCTCCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCAAAATAATCAAGTTTCATCGATATTTATTAATATTGCTCGTGTGATTATTTTGTTCGCTACa  >  4:75169/1‑148 (MQ=255)                                                                                                                                             |                                                                                                                                      AAATGCAACAGGTAAAGATTATTCAGGTACTGCGATGTATGCACAAGCCGGCATTGATAAAGCGTTCCATGGCAGTGGTTATCAATTTGATCCTACTTTCTCTGGCGTAGGTTCGTACTTTATTGCATTTGCTTTATTCTTCTTTGCATTTACTACAATTTTGTCGTACTACTACATTACAGAAACAAATGTTGCTTATTTAACGCGTAATCAAAATAATCAAGTTTCATCGATATTTATTAATATTGCTCGTGTGATTATTTTGTTCGCTACA  >  USA300TCH1516_ALE/1017538‑1017811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑