BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000731	8,740	T→C	I8V (ATT→GTT)	blaZ ←	beta‑lactamase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000731	8,740	0	T	C	99.2%	875.7 / ‑5.2	240	I8V (ATT→GTT)	blaZ	beta‑lactamase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (109/129); total (110/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

CTGAATTAAATTTTACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAATTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCTAAATTACTAAT  >  CP000731/8596‑8884

cTGAATTAAATTTTACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTTTTTTTTTTTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTaa                                                                                                                                               >  4:326744/1‑148 (MQ=255)

       aaaTTTTACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTTTTTTTTTTTTAACTCTATGGCATGTGAACTTATTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATAtt                                                                                                                                        >  4:120604/1‑148 (MQ=255)

        aaTTTTACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGTATTACATCCACTTAAAACTAAAGCAATTACAACTaaa                                                                                                                                              >  2:13864/1‑141 (MQ=255)

               cTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACttttt                                                                                                                                <  3:203070/148‑1 (MQ=255)

                ttCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTTTTTTTTATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTc                                                                                                                               >  4:91343/1‑148 (MQ=255)

                ttCCGTTCCACTTTTATTATTTAAAGCATAAACCCCAATATGTGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTaa                                                                                                                                      <  2:23046/141‑1 (MQ=255)

                       ccACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATA‑TTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATaaa                                                                                                                       <  3:116399/148‑1 (MQ=255)

                        cACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATaaa                                                                                                                       >  3:231188/1‑148 (MQ=255)

                         aCTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAAc                                                                                                                      >  4:23551/1‑148 (MQ=255)

                          cTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAAcc                                                                                                                     >  4:401412/1‑148 (MQ=255)

                               aGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCg                                                                                                                <  3:395662/148‑1 (MQ=255)

                                gTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTACAAATATTAACTTTTTCAAAATAAACCCTCCGa                                                                                                               <  4:230840/148‑1 (MQ=255)

                                gTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGAATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATTTGAACTTTTTCAAAATAAAc                                                                                                                      >  1:62504/1‑141 (MQ=255)

                                 tATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGat                                                                                                              >  3:157974/1‑148 (MQ=255)

                                 tATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGat                                                                                                              >  4:409317/1‑148 (MQ=255)

                                 tATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTATCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGat                                                                                                              <  3:217121/148‑1 (MQ=255)

                                  aTTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGata                                                                                                             <  4:356796/148‑1 (MQ=255)

                                  aTTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGata                                                                                                             <  3:55793/148‑1 (MQ=255)

                                  aTTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGata                                                                                                             <  3:184876/148‑1 (MQ=255)

                                  aTTTAAAGCATAAACACCAATATGAGCATTATA‑TTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGatat                                                                                                            <  4:29749/148‑1 (MQ=255)

                                        aGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAg                                                                                                       >  4:176834/1‑148 (MQ=255)

                                          cATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGatat                                                                                                            >  1:3323/1‑141 (MQ=255)

                                              aaCACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTTTaa                                                                                                 >  4:136642/1‑148 (MQ=255)

                                              aaCACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTaa                                                                                                 >  3:328791/1‑148 (MQ=255)

                                                cacCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAAtt                                                                                               >  3:160510/1‑148 (MQ=255)

                                                cacCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAAtt                                                                                               <  4:192455/148‑1 (MQ=255)

                                                 acCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAAttt                                                                                              <  3:7249/148‑1 (MQ=255)

                                                  ccAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGttt                                                                                                    >  1:15334/1‑140 (MQ=255)

                                                    aaTATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTta                                                                                           >  3:207894/1‑148 (MQ=255)

                                                    aaTATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTta                                                                                           <  3:347367/148‑1 (MQ=255)

                                                     atatGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGAAATGTGAAATGTTTGAATTAGATGCACTTAAAACTGAAGATATTAAAACTAAAGATATTAACTTTTTAAAATTAAACCGTGAGATATTAAAGTTGTaa                                                                                                 >  2:980/1‑141 (MQ=255)

                                                      tatGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAAt                                                                                                >  1:49911/1‑141 (MQ=255)

                                                         gAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAAt                                                                                      >  4:19323/1‑148 (MQ=255)

                                                          aGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAAttttt                                                                                            <  2:53099/141‑1 (MQ=255)

                                                          aGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATc                                                                                     >  4:5031/1‑148 (MQ=255)

                                                           gCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATGAACCCTCCGATATTACAGTTGTAATTTTTATAAATCa                                                                                    >  3:321305/1‑148 (MQ=255)

                                                            cATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCaa                                                                                   <  3:411568/148‑1 (MQ=255)

                                                                       ttttCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACaa                                                                        <  3:376417/148‑1 (MQ=255)

                                                                        tttCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAAt                                                                       >  3:84076/1‑148 (MQ=255)

                                                                              aaTCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAat                                                                 >  4:87112/1‑148 (MQ=255)

                                                                                tCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAAtt                                                                      <  1:19683/141‑1 (MQ=255)

                                                                                tCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAatat                                                               >  4:319391/1‑148 (MQ=255)

                                                                                 taTTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATAtt                                                              <  4:26973/147‑1 (MQ=255)

                                                                                  aTTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTg                                                             <  3:91343/148‑1 (MQ=255)

                                                                                     tAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATg                                                          <  3:140786/148‑1 (MQ=255)

                                                                                     tAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATg                                                          <  3:260936/148‑1 (MQ=255)

                                                                                     tAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATg                                                          <  3:118314/148‑1 (MQ=255)

                                                                                     tAACTCGTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACAAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCCCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATg                                                          <  4:382311/148‑1 (MQ=255)

                                                                                        ctctTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCa                                                       >  3:339984/1‑148 (MQ=255)

                                                                                         tctTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCaa                                                      >  4:337553/1‑148 (MQ=255)

                                                                                           tttGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAAtt                                                    <  4:223046/148‑1 (MQ=255)

                                                                                           tttGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAAtt                                                    <  4:259741/148‑1 (MQ=255)

                                                                                            ttGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTg                                                   <  4:101916/148‑1 (MQ=255)

                                                                                            ttGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTg                                                   <  4:125183/148‑1 (MQ=255)

                                                                                             tGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGt                                                  <  4:111422/148‑1 (MQ=255)

                                                                                              ggCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGtt                                                 <  3:87584/148‑1 (MQ=255)

                                                                                              ggCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGtt                                                 <  4:621/148‑1 (MQ=255)

                                                                                              ggCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGtt                                                 <  3:338034/148‑1 (MQ=255)

                                                                                              ggCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGtt                                                 <  3:233360/148‑1 (MQ=255)

                                                                                               gCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGtaa                                                       >  1:554/1‑139 (MQ=255)

                                                                                               gCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGttt                                                <  3:69228/148‑1 (MQ=255)

                                                                                                cATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTGCAACTAAATATACTAACTTTTCCAAAATAAACCCTCCGACATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTtta                                               <  4:83272/148‑1 (MQ=255)

                                                                                                cATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTtta                                               <  4:130507/148‑1 (MQ=255)

                                                                                                cATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTtta                                               <  4:65883/148‑1 (MQ=255)

                                                                                                 aTGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTttat                                              >  3:298597/1‑148 (MQ=255)

                                                                                                 aTGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTttat                                              >  4:302895/1‑148 (MQ=255)

                                                                                                 aTGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTttat                                              <  3:361155/148‑1 (MQ=255)

                                                                                                    tgAACCGTTTGAATTACATGCACTTAAAACTAAAACAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTtattat                                           <  4:237711/148‑1 (MQ=255)

                                                                                                     gAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATtata                                          <  3:19323/148‑1 (MQ=255)

                                                                                                      aaCTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATaa                                         <  3:53946/148‑1 (MQ=255)

                                                                                                      aaCTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATaa                                         <  3:274998/148‑1 (MQ=255)

                                                                                                      aaCTGTTTGAATTACATGCACTTAAAAACAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAACCAATAATATTACAATTGTAATATTGATGTCAATTGttt                                                <  1:8989/141‑1 (MQ=255)

                                                                                                          gTTTGAATTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAatat                                     <  3:225839/148‑1 (MQ=255)

                                                                                                                aTTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGaaa                               >  4:52717/1‑148 (MQ=255)

                                                                                                                aTTACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGaaa                               >  4:386241/1‑148 (MQ=255)

                                                                                                                  tACATGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAaaga                             <  4:205090/148‑1 (MQ=255)

                                                                                                                      tGCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGaa                                <  1:27799/141‑1 (MQ=255)

                                                                                                                       gCACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGgtg                        >  3:287895/1‑148 (MQ=255)

                                                                                                                        cACTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGgtgt                       <  4:247793/148‑1 (MQ=255)

                                                                                                                          cTTAAAACTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAaagaa                            >  2:21665/1‑141 (MQ=255)

                                                                                                                             aaaaCTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCaaaa                  >  4:282609/1‑148 (MQ=255)

                                                                                                                             aaaaCTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCaaaa                  >  4:304883/1‑148 (MQ=255)

                                                                                                                               aaCTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATg                <  3:124756/148‑1 (MQ=255)

                                                                                                                               aaCTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATg                <  3:407016/148‑1 (MQ=255)

                                                                                                                               aaCTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATg                >  3:24661/1‑148 (MQ=255)

                                                                                                                               aaCTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATg                <  4:228195/148‑1 (MQ=255)

                                                                                                                               aaCTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATg                <  3:137292/148‑1 (MQ=255)

                                                                                                                                aCTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATgg               <  3:264279/148‑1 (MQ=255)

                                                                                                                                aCTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATgg               <  4:160338/148‑1 (MQ=255)

                                                                                                                                 cTAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGc              >  4:69640/1‑148 (MQ=255)

                                                                                                                                  tAAAGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCt             <  4:242365/148‑1 (MQ=255)

                                                                                                                                   aaaGCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCTa            >  4:172538/1‑148 (MQ=255)

                                                                                                                                      gCAATTACAACTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCTAAAt         >  3:352446/1‑148 (MQ=255)

                                                                                                                                      gCAATTACAACTAAAAATATTAACGTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATg                >  1:44038/1‑141 (MQ=255)

                                                                                                                                        aattacaactaAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCTAAATTa       <  3:240279/148‑1 (MQ=255)

                                                                                                                                        aattacaactaAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCTAAATTa       <  3:388384/148‑1 (MQ=255)

                                                                                                                                         attacaactaAAAATATTAACTTTGTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCTAAATTAc      <  4:150352/148‑1 (MQ=255)

                                                                                                                                            acaactaAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATTTATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTAAAAGAAGGTTTCAAAATGGCTAAATTACtaa   >  4:41502/1‑148 (MQ=255)

                                                                                                                                             caactaAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCTAAATTACtaat  <  4:255569/148‑1 (MQ=255)

                                                                                                                                             caactaAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTATAAAAAAGGTGTCAAAATGGCTAAATTACtaat  >  4:322212/1‑148 (MQ=255)

CTGAATTAAATTTTACTTCCTTACCACTTTTAGTATTTAAAGCATAAACACCAATATGAGCATTATATTTTTTTTCTAAATCATTTAACTCTTTGGCATGTGAACTGTTTGAATTACATGCACTTAAAACTAAAGCAATTACAATTAAAAATATTAACTTTTTCAAAATAAACCCTCCGATATTACAGTTGTAATTTTTATAAATCAATAATATTACAATTGTAATATTGATGTCAATTGTTTATTATAAATATTTGAAAGAAGGTGTCAAAATGGCTAAATTACTAAT  >  CP000731/8596‑8884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`