Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,499,376	C→A	G883V (GGT→GTT)	ebh_1 ←	Extracellular matrix‑binding protein ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,499,376	0	C	A	100.0%	96.6 / NA	29	G883V (GGT→GTT)	ebh_1	Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/18);  total (11/18)

CGCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGTTTGATTTGT  >  USA300TCH1516_ALE/1499239‑1499518                                                                                                                                          |                                                                                                                                               cgCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAggtgg                                                                                                                                      <  3:16227/148‑1 (MQ=255)  gCCTCGATAGTATTTGGCATTTATTCCAAGAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAAGTTGTTTAACTAc                                                                                                                                            <  2:52142/141‑1 (MQ=255)  gCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAggtggt                                                                                                                                     <  4:387730/148‑1 (MQ=255)  gCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAggtggt                                                                                                                                     <  3:9400/148‑1 (MQ=255)          aGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATac                                                                                                                             <  3:42659/148‑1 (MQ=255)                  gCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAAt                                                                                                                     <  4:383380/148‑1 (MQ=255)                  gCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAAt                                                                                                                     <  4:348233/148‑1 (MQ=255)                    aTTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGAAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATacaac                                                                                                                          <  2:65672/141‑1 (MQ=255)                             aaCAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGt                                                                                                          >  4:111650/1‑148 (MQ=255)                             aaCAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGt                                                                                                          >  4:13954/1‑148 (MQ=255)                             aaCAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATGAGATTCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATATTGCGGTCGt                                                                                                          >  4:248479/1‑148 (MQ=255)                                      attgattgTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAAtt                                                                                                 <  3:406941/148‑1 (MQ=255)                                      attgattgTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAAtt                                                                                                 <  4:172355/148‑1 (MQ=255)                                      attgattgTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAAtt                                                                                                 <  4:197608/148‑1 (MQ=255)                                                      aTGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCAt                                                                                 >  4:203436/1‑148 (MQ=255)                                                          cATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAgg                                                                             <  4:291739/148‑1 (MQ=255)                                                             cTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGgtgt                                                                          >  3:271912/1‑148 (MQ=255)                                                                    aaTAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTc                                                                   <  4:360794/148‑1 (MQ=255)                                                                      tAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGc                                                                 <  3:279623/148‑1 (MQ=255)                                                                      tAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGc                                                                 <  3:71864/148‑1 (MQ=255)                                                                      tAGTAACAGCATTACTCATATTAGACGCACCTACTTTATTATAATAAACTACCGCTCTAGTTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGc                                                                 <  3:392436/148‑1 (MQ=255)                                                                                         atTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGAt                                              >  4:110226/1‑148 (MQ=255)                                                                                         atTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGAt                                              >  3:170996/1‑148 (MQ=255)                                                                                                gCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTAttt                                       >  4:155150/1‑148 (MQ=255)                                                                                                gCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTAttt                                       >  4:94820/1‑148 (MQ=255)                                                                                                                      ctactGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCAtt                 <  3:256707/148‑1 (MQ=255)                                                                                                                              ctAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGttt         <  3:124391/148‑1 (MQ=255)                                                                                                                                   ttGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGCGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGTTTGAttt    >  4:258100/1‑148 (MQ=255)                                                                                                                                     gTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGTTTGATttgt  >  3:364136/1‑148 (MQ=255)                                                                                                                                          |                                                                                                                                               CGCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGTTTGATTTGT  >  USA300TCH1516_ALE/1499239‑1499518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑