Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,830,883	C→T	K79K (AAG→AAA)	ezrA ←	Septation ring formation regulator EzrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,830,883	0	C	T	100.0%	91.3 / NA	26	K79K (AAG→AAA)	ezrA	Septation ring formation regulator EzrA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/16);  total (10/16)

ATCTTCTAATTGTTGCTGATAGCTTTGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGATTGCTTTTTC  >  USA300TCH1516_ALE/1830739‑1831026                                                                                                                                                 |                                                                                                                                                aTCTTCTAATTGTTGCTGATAGCTTTGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTa                                                                                                                                              >  3:349108/1‑148 (MQ=255)       tAATTGTTGCTGATAGCTTTGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTa                                                                                                                                        <  3:152539/148‑1 (MQ=255)       tAATTGTTGCTGATAGCTTTGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTa                                                                                                                                        <  3:230616/148‑1 (MQ=255)                                                    tGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGt                                                                                                  >  1:4207/1‑141 (MQ=255)                                                           tcaATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCtt                                                                                    <  3:14461/148‑1 (MQ=255)                                                            caATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCttt                                                                                   <  4:250382/148‑1 (MQ=255)                                                             aaTTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCtttt                                                                                  >  3:249929/1‑148 (MQ=255)                                                                   aCTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTc                                                                            <  3:49601/148‑1 (MQ=255)                                                                      ttGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATGATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCtttgta                                                                                >  1:59847/1‑138 (MQ=255)                                                                       tGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACt                                                                        <  4:221301/148‑1 (MQ=255)                                                                                                   ataaAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCaaa                                            <  3:51356/148‑1 (MQ=255)                                                                                                    taaAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCaaaa                                           >  3:74595/1‑148 (MQ=255)                                                                                                     aaaGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCTTTGCATTGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAg                                          >  4:56281/1‑148 (MQ=255)                                                                                                     aaaGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAg                                          >  4:401200/1‑148 (MQ=255)                                                                                                     aaaGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCATTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAg                                          >  3:223478/1‑148 (MQ=255)                                                                                                          cTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAAc                                     <  3:293584/148‑1 (MQ=255)                                                                                                            cAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAg                                          >  1:56185/1‑141 (MQ=255)                                                                                                              gCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGtct                                 <  4:214624/148‑1 (MQ=255)                                                                                                                      gggtttttCTTCCTCAGTAGCTAGATATTGAGGTTTACGGTCTACTTTGTCCTTTGTCAGTGCATCGTAGTTCGTTTTTGTGTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCa                         <  3:331345/145‑1 (MQ=255)                                                                                                                         tttttCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCAttt                      <  4:249929/148‑1 (MQ=255)                                                                                                                                  cacaGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCg             <  4:298933/148‑1 (MQ=255)                                                                                                                                     aGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGAtt          <  3:159445/148‑1 (MQ=255)                                                                                                                                     aGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGAtt          <  3:148220/148‑1 (MQ=255)                                                                                                                                         gCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGATTGCtt      <  3:223090/148‑1 (MQ=255)                                                                                                                                            aGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCTCCTTTTAAATTCAACTTAGATAATTGTGCACGGTTTTGATCAAAAGGTAACGTGTCAATTTCATTTTTACTTTCGATTGCttttt   >  4:110996/1‑148 (MQ=255)                                                                                                                                             gATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGATTGCTTTTTc  <  4:140216/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                ATCTTCTAATTGTTGCTGATAGCTTTGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGATTGCTTTTTC  >  USA300TCH1516_ALE/1830739‑1831026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑