Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,079,114	T→C	H201R (CAC→CGC)	natA_1 ←	ABC transporter ATP‑binding protein NatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,079,114	0	T	C	100.0%	87.4 / NA	25	H201R (CAC→CGC)	natA_1	ABC transporter ATP‑binding protein NatA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/15);  total (10/15)

AAATCCCGTACGCTTATGTTCTTCATAAATAAGTAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGTGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAGTAGGCTC  >  USA300TCH1516_ALE/2078972‑2079256                                                                                                                                               |                                                                                                                                               aaaTCCCGTACGCTTATGTTCTTCATAAATAAGTAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATg                                                                                                                                           >  3:71099/1‑148 (MQ=255)              ttATGTTCTTCATAAATAAGTAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTa                                                                                                                              >  4:381433/1‑148 (MQ=255)                    tcttcATAAATAAGTAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTAtttttt                                                                                                                        >  4:182866/1‑148 (MQ=255)                                  tAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATa                                                                                                          >  4:259695/1‑148 (MQ=255)                                              ttCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGAc                                                                                              >  3:260007/1‑148 (MQ=255)                                               tcatcatcaAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAAtgtgt                                                                                                    >  2:18756/1‑141 (MQ=255)                                                                  tAGCCCCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTATATTTtc                                                                          <  3:395163/148‑1 (MQ=255)                                                                  tAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTTAGATAATGTGTGTCGACATGAATATTGTTTTATTTtc                                                                          <  4:374043/148‑1 (MQ=255)                                                                  tAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTtc                                                                          <  3:209210/148‑1 (MQ=255)                                                                       ccacTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTATATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCaa                                                                     >  4:265175/1‑148 (MQ=255)                                                                                aCTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCtt                                                            <  3:182866/148‑1 (MQ=255)                                                                                 cTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTg                                                           >  4:353922/1‑148 (MQ=255)                                                                                                 cAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAAt                                           <  3:49537/148‑1 (MQ=255)                                                                                                   gttgttCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAg                                         <  4:395140/148‑1 (MQ=255)                                                                                                   gttgttCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAg                                         <  4:222248/148‑1 (MQ=255)                                                                                                   gttgttCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAg                                         <  4:26103/148‑1 (MQ=255)                                                                                                                 ccATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTTTTTTTCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTAATCAATTAATTATTGCTGAATTATATCCAATAGCTCATTTTTAGcaa                           >  4:15863/1‑146 (MQ=255)                                                                                                                      tAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGa                      <  4:236498/148‑1 (MQ=255)                                                                                                                      tAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGa                      <  3:149478/148‑1 (MQ=255)                                                                                                                          aTAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCaa                  >  3:179217/1‑148 (MQ=255)                                                                                                                                   ccGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAg         <  4:11379/148‑1 (MQ=255)                                                                                                                                   ccGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAg         <  4:391294/148‑1 (MQ=255)                                                                                                                                   ccGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAg         <  4:398263/148‑1 (MQ=255)                                                                                                                                      tCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCt             <  2:11179/141‑1 (MQ=255)                                                                                                                                          ttAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAGTAGGCtc  <  3:122179/148‑1 (MQ=255)                                                                                                                                               |                                                                                                                                               AAATCCCGTACGCTTATGTTCTTCATAAATAAGTAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGTGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAGTAGGCTC  >  USA300TCH1516_ALE/2078972‑2079256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑