BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	405,184	C→A	5.8%	W61L (TGG→TTG)	USA300HOU_RS01870 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	405,184	0	C	A	5.8%	295.7 / 4.6	96	W61L (TGG→TTG)	USA300HOU_RS01870	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (50/40); new base A (3/3); total (53/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

AGAATGTCTTTTCAAAGTCCGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATCAAAGAACAACTCATGGAATCCTGCA  >  USA300TCH1516_ALE/405044‑405324

agaaTGTCTTTTCAAAGTCCGGATATGGGCTGTTAGATAGGAAGACGATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCCGATTTAcc                                                                                                                                              >  2:403361/1‑141 (MQ=255)

 gaaTGTCTTTTCAAAGTCCGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCa                                                                                                                                             >  1:958627/1‑141 (MQ=255)

     gTCTTTTCAAAGTCCGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATaaa                                                                                                                                         >  2:165130/1‑140 (MQ=255)

      tCTTTTCAAAGTCCGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGa                                                                                                                                        <  2:898043/141‑1 (MQ=255)

       cTTTTCAAAGTCCGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGaa                                                                                                                                       <  2:866931/141‑1 (MQ=255)

        ttttCAAAGTCCGGATATAGGCTGTTAGATGGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAAc                                                                                                                                      >  1:876850/1‑141 (MQ=255)

          ttCAAAGTCCGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACaa                                                                                                                                    >  2:666246/1‑141 (MQ=255)

           tCAAAGTCCGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACaaa                                                                                                                                   >  1:584864/1‑141 (MQ=255)

             aaaGTCCGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAAcc                                                                                                                                 >  1:423307/1‑140 (MQ=255)

              aaGTCCGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTc                                                                                                                                >  1:229651/1‑141 (MQ=255)

               aGTCCGGATATAGGCTGTTAGATAGGAAGACCATGGTGAACATATTAAACATCATAAACGAGGCGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACAACATTAACCATAAGAACAAACTCa                                                                                                                               >  2:632125/1‑141 (MQ=255)

               aGTCCGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTGACCATAAGAACAAACTCa                                                                                                                               >  1:656738/1‑141 (MQ=255)

                  ccGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGa                                                                                                                            >  1:259357/1‑141 (MQ=255)

                  ccGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGa                                                                                                                            <  1:346140/141‑1 (MQ=255)

                   cGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGAc                                                                                                                           <  1:463042/141‑1 (MQ=255)

                   cGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGAc                                                                                                                           <  1:335068/141‑1 (MQ=255)

                         taGGCTGTTAGATAGGAAGACCATGATGAACATATTAGACAGCATAGACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGAAGATGTa                                                                                                                     >  2:886971/1‑141 (MQ=255)

                         taGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTa                                                                                                                     <  1:734077/141‑1 (MQ=255)

                            gCTGTTAGATAGGAAGACCCTGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTAttt                                                                                                                  >  1:637155/1‑141 (MQ=255)

                             cagTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGACTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTg                                                                                                                 <  1:803849/139‑1 (MQ=255)

                              tcttAGATATGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCCATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGa                                                                                                                <  2:512052/139‑1 (MQ=255)

                              tGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCGCATTAACCATAAGAACAATCTCATGACGATGTATTTGa                                                                                                                <  1:591629/141‑1 (MQ=255)

                              tGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGa                                                                                                                <  1:617619/141‑1 (MQ=255)

                                 tAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGaaaa                                                                                                             <  1:759512/141‑1 (MQ=255)

                                 tAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGaaaa                                                                                                             <  2:864708/141‑1 (MQ=255)

                                 tAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGaaaa                                                                                                             <  1:998072/141‑1 (MQ=255)

                                   gATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCTTGACGATGTATTTGaaaaaa                                                                                                           >  1:596858/1‑140 (MQ=255)

                                   gATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACAATAAGAACAAACTCATGACGATGTATTTGAAaaat                                                                                                           >  1:985303/1‑141 (MQ=255)

                                   gATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACAATAAGAACAAACTCATGACGATGTATTTGAAaaat                                                                                                           >  1:1056642/1‑141 (MQ=255)

                                      aGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAGCCATAAGAACAAACTCATGACGATGTATTTGAAaaataaa                                                                                                        >  2:541600/1‑141 (MQ=255)

                                       ggAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAaaataaat                                                                                                       >  1:6942/1‑141 (MQ=255)

                                       ggAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACGTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAaaataaat                                                                                                       >  1:943637/1‑141 (MQ=255)

                                               atgatgAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGaa                                                                                               >  2:145329/1‑141 (MQ=255)

                                               atgatgAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGaa                                                                                               >  2:640861/1‑141 (MQ=255)

                                               atgatgAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGaa                                                                                               >  1:23499/1‑141 (MQ=255)

                                                tgatgaACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGaaa                                                                                              >  2:64333/1‑141 (MQ=255)

                                                 tatgaACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAAt                                                                                             <  1:475011/140‑1 (MQ=255)

                                                 gatgaACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAAt                                                                                             <  1:506479/141‑1 (MQ=255)

                                                 gatgaACACAGTAAACCTCAGAAACCAGACGACTTTGNATGGAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACNAACTCATGACTATGTATTTGAAAAATAAATCAGCTGAAAt                                                                                             <  2:558651/141‑1 (MQ=255)

                                                    gaACATATTAAACATCATAAACGTGACGTCTTTGAATGTAACTTGACCAAGTCGATTTGTAAAAAATGTTTGATGAGACCACATTAACAATAAGAACAAACTTATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGa                                                                                          <  2:532119/141‑1 (MQ=255)

                                                    gaACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGa                                                                                          <  1:937226/141‑1 (MQ=255)

                                                    gaACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGa                                                                                          <  1:725994/141‑1 (MQ=255)

                                                    gaACATAACAAACATAATAAACGATAAGTCTTTTAAGTTAACTTGACCAAAACAAAATAAAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGa                                                                                          <  2:206179/141‑1 (MQ=255)

                                                     aaCATATTAAACATCTTAAACGAGACGTCTTTGAGTGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCCTAAGAACCAACTCATGACGATGTATTTGAAATATAAATCAGCTGAAATGGaa                                                                                         <  1:645971/141‑1 (MQ=255)

                                                     aaCATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCCGCTGAAATGGaa                                                                                         >  1:316474/1‑141 (MQ=255)

                                                          atTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCTATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGtt                                                                                    <  1:520136/141‑1 (MQ=255)

                                                           ttAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGttt                                                                                   >  2:294108/1‑141 (MQ=255)

                                                             aaaCATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGT‑AAAAATGTTTGATGGGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAACCAGCTGAAATGGAACGGTTTtgt                                                                                >  1:493826/1‑141 (MQ=255)

                                                              aaCATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACCCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTtgt                                                                                >  2:444951/1‑141 (MQ=255)

                                                                cctcatAAACGCGACGTCTTTGCATCTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTgtgt                                                                              <  1:884359/139‑1 (MQ=255)

                                                                catcatAAACGAGACGTCTTTGAATGTAACTTGACCCAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTgtgt                                                                              <  1:989756/141‑1 (MQ=255)

                                                                catcatAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTgtgt                                                                              <  1:13810/141‑1 (MQ=255)

                                                                 ttcatAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACTATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGtgtt                                                                             <  2:664009/140‑1 (MQ=255)

                                                                  tcatAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGtgatg                                                                            >  1:189909/1‑141 (MQ=255)

                                                                    atAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATTAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTgttgtt                                                                          >  2:511722/1‑141 (MQ=255)

                                                                    atAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAAGGGAACCGTTTTGTgttgtt                                                                          >  1:51010/1‑141 (MQ=255)

                                                                        aCGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAGTAAATCAGCTGAAATGGAGCCGTTTTGTGATTTTaaga                                                                      >  1:22426/1‑139 (MQ=255)

                                                                         agagaCGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAAt                                                                     <  2:876850/140‑1 (MQ=255)

                                                                              cGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTCAAATCACAt                                                                >  1:354442/1‑141 (MQ=255)

                                                                               gTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACAtg                                                               >  1:773258/1‑141 (MQ=255)

                                                                                tCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTGACCATAAGAACAAACTCATGACGATGTATTTGAAAAATACATCAGCTGAAGTGGACCCGTTTTGGGTTGTTAGAATCACAtgg                                                              >  1:193815/1‑140 (MQ=255)

                                                                                tCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACAtgt                                                              <  1:316717/141‑1 (MQ=255)

                                                                                tCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGGTGTTAAAATCACAtgt                                                              >  2:536392/1‑141 (MQ=255)

                                                                                 cTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACGGTTTTGTGTTGTTAAAATCACAtgtg                                                             <  2:637155/141‑1 (MQ=255)

                                                                                 cTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACAtgtg                                                             <  1:132373/141‑1 (MQ=255)

                                                                                 cTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACAtgtg                                                             >  2:531635/1‑141 (MQ=255)

                                                                                  tttGAATGTAACTTTACCACATCGATTTGTAAAAAAAGTTTCATGAGACCACAGTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGc                                                            <  2:843925/141‑1 (MQ=255)

                                                                                  tttGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGGGc                                                            >  1:1061351/1‑141 (MQ=255)

                                                                                    tGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCaa                                                          <  1:613955/141‑1 (MQ=255)

                                                                                    tGAACGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAACAATAAATCCGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCaa                                                          <  2:434183/141‑1 (MQ=255)

                                                                                       aTGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAAttt                                                       <  1:152728/141‑1 (MQ=255)

                                                                                       aTGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAAttt                                                       <  2:590124/141‑1 (MQ=255)

                                                                                        tGTAACTTGACCAAATCGATGTTTAAAAAATGATTGATGAGACCACATTAACAATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATAACATGTAAAAtttt                                                      >  1:210012/1‑141 (MQ=255)

                                                                                         gTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAAttttt                                                     >  1:612853/1‑141 (MQ=255)

                                                                                         gTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAAttttt                                                     >  2:1008310/1‑141 (MQ=255)

                                                                                             cTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGCAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCGATTTTTTGaa                                                 >  1:577517/1‑141 (MQ=255)

                                                                                             cTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAAttttttttt                                                 >  2:791059/1‑138 (MQ=255)

                                                                                             cTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGaa                                                 >  1:1055334/1‑141 (MQ=255)

                                                                                              ctGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAACGGAACCGTTTTGTGTAGTTCAAATCACATGAGCAAttatttatat                                                >  2:174332/2‑137 (MQ=255)

                                                                                                 aCCCAATCGATTTGTAATAAATGTTTTATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGc                                             <  2:392261/141‑1 (MQ=255)

                                                                                                       tCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGAc                                       >  2:402734/1‑141 (MQ=255)

                                                                                                           tttGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATCAATCAGCTGAAATGGCACTGCTTTGTGTTGTTAAAATCACATGTGCAATTTATTGAATGGCATAGACGaaa                                   >  1:993241/1‑141 (MQ=255)

                                                                                                              gTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTCCAATTTTTTGAATGGCACAGACGAAAAtt                                >  2:517740/1‑141 (MQ=255)

                                                                                                                   aaaTGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGGACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATc                           >  1:955284/1‑141 (MQ=255)

                                                                                                                   aaaTGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTCGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATc                           <  2:582153/141‑1 (MQ=255)

                                                                                                                      tGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATCaaa                        <  1:363502/141‑1 (MQ=255)

                                                                                                                        tttGATGAGACCACATTAACAATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATCAAAGa                      <  2:985303/141‑1 (MQ=255)

                                                                                                                        tttGATGAGACCACATTAACAATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATCAAAGa                      <  2:1056642/141‑1 (MQ=255)

                                                                                                                          tgatgaGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATCAAAGaac                    >  1:652644/1‑141 (MQ=255)

                                                                                                                            atgaGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCCTAGACGAATGTTAAATCAAAGaacaa                  <  2:229651/141‑1 (MQ=255)

                                                                                                                                 aCCACATTAACCATAAGAACAAACTCATGACGATGTATTTTAAAAATAAATCCGCTGAAATGGAACCGTGTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATCAAAGAACAACTCAt             <  2:316474/141‑1 (MQ=255)

                                                                                                                                   cacaTTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTTAATCAAAGAACAACTCATgg           <  1:643577/141‑1 (MQ=255)

                                                                                                                                    acaTTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGATTTTTATTGACGAAAAGTAAAGCAAAGGACAACTCATGGa          >  2:206513/1‑141 (MQ=255)

                                                                                                                                         aaCCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTCTTGTGTGGTTAAAATCACATGGGCAATTTTTTGAGTGGCATAGACGAAAAGTAAATCAAAGAGCAACTGAGGGAAGCCt     >  1:801175/1‑141 (MQ=255)

                                                                                                                                         aaCCATAAGAACAAACACATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATCAAAGAACAACTCATGGAATCCt     >  2:230106/1‑141 (MQ=255)

                                                                                                                                          aCCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATCAAAGAACAACTCATGGAATCCTg    >  1:80763/1‑141 (MQ=255)

                                                                                                                                          aCCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATCAAAGAACAACTCATGGAATCCTg    >  1:736001/1‑141 (MQ=255)

                                                                                                                                            cATAAGAACAAACTTATGAACATGTATTTGAAAAATAAATCATCTGAAATTGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATCAAAGAACAACTCATGGAATCCTGCa  <  2:304878/141‑1 (MQ=255)

AGAATGTCTTTTCAAAGTCCGGATATAGGCTGTTAGATAGGAAGACCATGATGAACATATTAAACATCATAAACGAGACGTCTTTGAATGTAACTTGACCAAATCGATTTGTAAAAAATGTTTGATGAGACCACATTAACCATAAGAACAAACTCATGACGATGTATTTGAAAAATAAATCAGCTGAAATGGAACCGTTTTGTGTTGTTAAAATCACATGTGCAATTTTTTGAATGGCATAGACGAAAATTAAATCAAAGAACAACTCATGGAATCCTGCA  >  USA300TCH1516_ALE/405044‑405324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`