BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	434,310	A→T	7.5%	L72* (TTA→TAA)	USA300HOU_RS02040 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	434,310	0	A	T	7.5%	270.5 / 2.6	108	L72* (TTA→TAA)	USA300HOU_RS02040	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (42/53); new base T (7/2); total (50/58)
Fisher's exact test for biased strand distribution p-value = 8.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ACCAAGTAGGTGACGTTCTTCGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGTAACCACGTTCT  >  USA300TCH1516_ALE/434171‑434450

aCCAAGTAGGTGACGTTCTTCGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTaa                                                                                                                                             <  1:374021/141‑1 (MQ=255)

 ccAAGTAGGTGACGTGCTTCGCGCATTGTCTGGATTTCTAGGCGTAAATCTTCGACTAGTTGAGTGACATTGTTATTAGTAGGGAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAAt                                                                                                                                            <  1:216935/141‑1 (MQ=255)

   aaGTAGGTGGCGTTCTTCGCGCATTTTTCTGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATtc                                                                                                                                          <  1:103178/141‑1 (MQ=255)

   aaGTAGGGGACGTTCTTCGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATtc                                                                                                                                          <  2:953099/141‑1 (MQ=255)

         gTGACGTTCTTCGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTTTAATTCATCaaa                                                                                                                                    >  2:548528/1‑141 (MQ=255)

         gTGACGTTCTTCGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCaaa                                                                                                                                    >  2:355984/1‑141 (MQ=255)

          tGACGTTCTTCGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGCTATTTTAATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAAc                                                                                                                                   <  2:640258/141‑1 (MQ=255)

          tGACGTTCTTCGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAAc                                                                                                                                   >  2:309189/1‑141 (MQ=255)

             cGTTCTTCGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCt                                                                                                                                <  1:920967/141‑1 (MQ=255)

              gTTCTTCGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTa                                                                                                                               <  2:769515/141‑1 (MQ=255)

              gTTCTTCGCGCATTTTCTGAATTTCTAGGCGTAAATCTTCTACTCATTGAGCGCCATTTTGATTAGTAGGAAAGTTTTTGTCATCGTTTTTGACAATGACTTGCGGGAAGTCTTTTTATTTTTGTAATTAATAAAACGCtg                                                                                                                               >  2:447302/1‑140 (MQ=255)

                 cttcGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGAt                                                                                                                            >  2:196927/1‑141 (MQ=255)

                 cttcGCGCATTTTCTGGAGTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCCAACGCTAGAt                                                                                                                            >  2:802128/1‑141 (MQ=255)

                   tcGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCa                                                                                                                          >  1:1064619/1‑141 (MQ=255)

                        cATTTTCTGGATTTCTAGGCGTAAATCTTCTACTTGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACt                                                                                                                     <  2:257825/141‑1 (MQ=255)

                        cATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACt                                                                                                                     <  1:633330/141‑1 (MQ=255)

                        cATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACt                                                                                                                     <  2:291924/141‑1 (MQ=255)

                          ttttCTGGATTTCTAGGCGTAAATCTTCTCCTAGTTGAGCGACATTTTGATTAGTAGGGAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTAt                                                                                                                   <  1:502658/141‑1 (MQ=255)

                                  agttCTAGGCGTAAATCTTCTACTATTTGAGTGACATTTTTATTTGTAGGTAAGTTTTTGTCATCGTTTTTGAGAATTACTTGCAGGAGGTCTCGTTCTTTTTCTTATTCATCAAGCGCTAGATCATAACTATttgtttgt                                                                                                           <  2:3798/139‑1 (MQ=255)

                                    ttCTAGGCGTAAATCTTCTGTTAGGGAAGCGACATTTTGCTTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTTTTTCATCTAACGCTGGATCATAACTCTTTgtttgttt                                                                                                         >  2:228808/1‑141 (MQ=255)

                                    ttCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTgtttgttt                                                                                                         >  2:51599/1‑141 (MQ=255)

                                     tCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTTTTTTTTTTTTGCAGGAAGTGTTTTTCTTTTTCTAATTCGTCATACGCTGGATAATAACTCtttgttttattt                                                                                                        >  2:746187/1‑136 (MQ=255)

                                     tCTAGGCGTAAATCTTCTACTAGTTGAGCAACATTTTAATAAATACGTACGTTTTTGTCATAGACTTGGAAAATGAATTGTAGGAAGACTGTTTGATTTTCTAATTCATCAAAATCTAGATAATAACTTTTGGCTTGtttt                                                                                                        >  2:510433/1‑141 (MQ=255)

                                      cTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTa                                                                                                       <  2:26449/141‑1 (MQ=255)

                                       tAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTGCGTTTTTGTTTTCTTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAGTTCGTGAACCGCTCGATCATTACTATTTGTTTGTTTTat                                                                                                      >  2:691496/1‑140 (MQ=255)

                                       tAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTAc                                                                                                      >  2:87087/1‑141 (MQ=255)

                                       tAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTCTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAGCGCTAGATCATAACTATTTGTTTGTTTTAc                                                                                                      <  1:543097/141‑1 (MQ=255)

                                          gCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTTATTTTTTTTGACAATTACTTGCAGGAAGTCTTTTTTTTTTTTTTTTTAATCAAATGCTGGATCATAACTATTTGTTTTTTTTAtttt                                                                                                   >  2:483465/1‑141 (MQ=255)

                                                aTCTTCTCCTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTTTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGc                                                                                             <  2:427351/141‑1 (MQ=255)

                                                aTCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGc                                                                                             <  2:321946/141‑1 (MQ=255)

                                                 tcttctACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGGCTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAATCGCTAGATCATAACTATTTGTTTGTTTTTCTTTTTCggta                                                                                            >  1:369553/1‑139 (MQ=255)

                                                 tcttctACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTTTTATTTTTTCAACGCTAGATCATAACTATTTTTTTGTTTTCCTTTTTAGGCa                                                                                            >  2:164103/1‑141 (MQ=255)

                                                 tcttctACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCa                                                                                            >  1:583204/1‑141 (MQ=255)

                                                  cttctACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCATACGCTGGTTCATAGCTATTTGTTTGTTTTACTTTGTCGGctt                                                                                           >  1:323705/1‑139 (MQ=255)

                                                  cttctACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCCTAACTATTTGTTTGTTTTACTTTGTCGGCaa                                                                                           >  2:919369/1‑141 (MQ=255)

                                                     ttactaGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGt                                                                                        <  1:628734/140‑1 (MQ=255)

                                                         taGTTGGGCGACATTTTGATTAGTTGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCttt                                                                                    <  1:289912/141‑1 (MQ=255)

                                                         taGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCCGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTTTCGGCATTGTCttt                                                                                    >  1:145870/1‑141 (MQ=255)

                                                          aGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTTTTTTTTTTTTTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTTTTTTTTCGGCAATGTCtttt                                                                                   >  2:734335/1‑140 (MQ=255)

                                                          aGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTg                                                                                   >  1:102700/1‑141 (MQ=255)

                                                           gTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGa                                                                                  <  1:951626/141‑1 (MQ=255)

                                                           gTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGa                                                                                  <  1:753925/141‑1 (MQ=255)

                                                            ttGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGaa                                                                                 <  1:773256/141‑1 (MQ=255)

                                                                 cGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCt                                                                            >  2:932791/1‑141 (MQ=255)

                                                                 cGACATTTTGATTAGTAGGTAAGTGTTTGTCTTCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCt                                                                            <  2:856607/141‑1 (MQ=255)

                                                                    cATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTACGCAATGTCTTTGAAACGATCGa                                                                         >  2:939589/1‑141 (MQ=255)

                                                                    cATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTAGCAGGAAGTCTTTTTCTTTTTCTAATTTATTAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCACTGACTTTGAAAAGCTCGa                                                                         >  2:686376/1‑141 (MQ=255)

                                                                     aTTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTTTCGGCAATGTCTTTCAAAAGCTCGat                                                                        >  2:143319/1‑141 (MQ=255)

                                                                     aTTTTGATTAGGAGGTAAGTTTTTTTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGat                                                                        <  2:386500/141‑1 (MQ=255)

                                                                      ttttGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGata                                                                       <  1:323371/141‑1 (MQ=255)

                                                                         tGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAACTCGATAtct                                                                    <  2:625878/141‑1 (MQ=255)

                                                                            ttAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTTTTTTTTTTTTTCATCAAACGCTAGATCCTAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTTACACGCTCGATAtcttct                                                                 >  2:624446/1‑141 (MQ=255)

                                                                            ttAGTAGGTAAGTGTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATAtcttct                                                                 <  2:823322/141‑1 (MQ=255)

                                                                             tagtagGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATAtcttctt                                                                <  1:432678/141‑1 (MQ=255)

                                                                                    tAAGTTTTTGTCATCGTTTTTGACAATGACCTGCAGGAAGTCTTTTTCTTTTTCTAATTCAGCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGaa                                                         <  1:228808/141‑1 (MQ=255)

                                                                                    caaGCTTTTGTCATCGTTTTTGCTAATCACTTGCAGTGATTCTTTTCCTTTTTCTCATTCATCAACCGCTAGATCATAGCTATTTGTCTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGaa                                                         <  2:318290/140‑1 (MQ=255)

                                                                                       gTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTGGTTTTACTTTGTGGGCACTGTCTTTGAAAAGCTCGATATCTTCTTATTTGAAATc                                                      >  2:504984/1‑141 (MQ=255)

                                                                                          tctGTCACCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGt                                                   <  1:34870/139‑1 (MQ=255)

                                                                                            tGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGCTCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGTAATCAGTTg                                                 >  1:359637/1‑141 (MQ=255)

                                                                                            tGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGttt                                                 >  2:101229/1‑140 (MQ=255)

                                                                                            tGTCATCGGTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTCATTCATCAAACGCTAGATCACAACTATTTGTTTGTTTTCCTTTGTCGGCAATATCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTg                                                 >  2:46677/1‑141 (MQ=255)

                                                                                             gTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGc                                                >  1:828559/1‑141 (MQ=255)

                                                                                               cATCGTTTTTTACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCtt                                              <  2:633575/141‑1 (MQ=255)

                                                                                               cATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTTTTATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTTTCGGCAATGTCTTTGAAAATCTCGATATCTTCTTCTTTGAAATCAGTTGttt                                              >  1:1000068/1‑141 (MQ=255)

                                                                                               cATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGATATCAGTTGCtt                                              >  1:49843/1‑141 (MQ=255)

                                                                                               cATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCtt                                              <  2:764317/141‑1 (MQ=255)

                                                                                                 tCGTTTTTGACAAGGAGTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATTATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCa                                            <  2:945873/141‑1 (MQ=255)

                                                                                                     ttttGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGAc                                        <  1:326139/141‑1 (MQ=255)

                                                                                                     ttttGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGAc                                        <  1:1010031/141‑1 (MQ=255)

                                                                                                       ttGACAATGACTTGCAGGAAGTTTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGGCCa                                      <  2:651630/141‑1 (MQ=255)

                                                                                                        tgatcaTGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGCAATCCGTTGCTTCACGACCAc                                     <  1:545587/136‑1 (MQ=255)

                                                                                                        tGACAATGACATGCGGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGGAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACTAcccc                                     >  2:719157/1‑139 (MQ=255)

                                                                                                         gACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCAca                                    >  2:516161/1‑140 (MQ=255)

                                                                                                          aCAATGCCTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGa                                   <  2:548609/141‑1 (MQ=255)

                                                                                                             aTGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGCAATCAGTTGCTTAACTACCACGatat                                >  2:860010/1‑141 (MQ=255)

                                                                                                               gACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTc                              <  2:93674/141‑1 (MQ=255)

                                                                                                               gACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTc                              <  1:773623/141‑1 (MQ=255)

                                                                                                                   tGCAGATAGTCTTTTTCCTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTc                          <  2:770452/141‑1 (MQ=255)

                                                                                                                           gTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCtttg                  >  1:959240/1‑139 (MQ=255)

                                                                                                                           gTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAg                  >  2:130704/1‑141 (MQ=255)

                                                                                                                           gTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGATTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTat                  >  1:826094/1‑140 (MQ=255)

                                                                                                                             ttttttctttttcTTATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTTAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGtt                <  2:361613/140‑1 (MQ=255)

                                                                                                                             ctttttctttttTTATTTTATCAAATGCTAGTTCATAACTATTTGTTGTTTTTACTTTGTCGGCAATGTCTTTGAAAAGGTGGATTTCTTCTTCTTTGAAATCAGTTGCTTCACGCCCACGATATTCAGTCTTGCTTAGtt                <  2:432065/141‑1 (MQ=255)

                                                                                                                             ctttttctttttCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTGTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAggt                <  2:784055/141‑3 (MQ=255)

                                                                                                                              tctttctttttcgGGTTCATCATGCTCTAGATTTTAACTATTTCTTTGTTTTACTTTGTCGGCTATGTCTTTGAGTAGCTCGATATCTTCTTCTTTGAATTCTGTTTCTTCCCGACCACGATATTCAGTCTTGCTTAGTTg               <  2:766349/139‑1 (MQ=255)

                                                                                                                                ttttttttttttttTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTTAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGt             >  2:334095/1‑141 (MQ=255)

                                                                                                                                tttctttttctAATTCGTCAAACGCTAGATCATGACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGt             <  2:390830/141‑1 (MQ=255)

                                                                                                                                tttctttttctAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGt             <  2:390928/141‑1 (MQ=255)

                                                                                                                                 ttctttttctCATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGTa            <  2:1021941/141‑1 (MQ=255)

                                                                                                                                 ttctttttctAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTTAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGTa            <  1:345647/141‑1 (MQ=255)

                                                                                                                                 ttctttttctAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATCTTCAGTCTTGCTTAGTTGGTa            >  2:560567/1‑141 (MQ=255)

                                                                                                                                 ttctttttctAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGtt            >  1:742998/1‑140 (MQ=255)

                                                                                                                                 ttctttttctAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGTa            <  1:151639/141‑1 (MQ=255)

                                                                                                                                  tctttttctAATTCATCAAACGCTAGATCATTACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGTaa           <  1:904241/141‑1 (MQ=255)

                                                                                                                                  tctttttctAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGTTATTCAGTCTTTCTTAGTTGGTaa           >  1:6910/1‑141 (MQ=255)

                                                                                                                                   ctttttctAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGTAAc          <  1:355984/141‑1 (MQ=255)

                                                                                                                                      tttctAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGTAACCAc       >  2:697337/1‑141 (MQ=255)

                                                                                                                                       ttctAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGTAACCACg      >  1:753357/1‑141 (MQ=255)

                                                                                                                                        tctAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGTAACCACGt     >  1:809027/1‑141 (MQ=255)

                                                                                                                                           aaTTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGCTTTGCATATTTGGTAACCACGttct  <  2:1024732/141‑1 (MQ=255)

ACCAAGTAGGTGACGTTCTTCGCGCATTTTCTGGATTTCTAGGCGTAAATCTTCTACTAGTTGAGCGACATTTTGATTAGTAGGTAAGTTTTTGTCATCGTTTTTGACAATGACTTGCAGGAAGTCTTTTTCTTTTTCTAATTCATCAAACGCTAGATCATAACTATTTGTTTGTTTTACTTTGTCGGCAATGTCTTTGAAAAGCTCGATATCTTCTTCTTTGAAATCAGTTGCTTCACGACCACGATATTCAGTCTTGCTTAGTTGGTAACCACGTTCT  >  USA300TCH1516_ALE/434171‑434450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`