Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,265,591 C→T 7.9% intergenic (+99/‑74) femA_1 → / → dprA Aminoacyltransferase FemA/DNA processing protein DprA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,265,5910CT 7.9% 194.1 / 5.9 78intergenic (+99/‑74)femA_1/dprAAminoacyltransferase FemA/DNA processing protein DprA
Reads supporting (aligned to +/- strand):  ref base C (37/32);  new base T (3/3);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

AATTCTATATAAATTTGCAAAACTTATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGACTAAACAAATTCATCAATT  >  USA300TCH1516_ALE/1265453‑1265729
                                                                                                                                          |                                                                                                                                          
aaTTCTATATAAATTTGCAAAACTTATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACaa                                                                                                                                          <  2:715903/141‑1 (MQ=255)
 aTTCTATATAAATTTGCAAAACTTATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTATTCTTTAAAATAAAATTAAATAGACAAt                                                                                                                                         >  2:426414/1‑141 (MQ=255)
     tatataAATTTGCAAAACTTATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACACTGttt                                                                                                                                     >  2:105083/1‑141 (MQ=255)
     tatataAATTTGCAAAACTTATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGttt                                                                                                                                     >  2:296197/1‑141 (MQ=255)
     tatataAAATGGCAAAACTTATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGttt                                                                                                                                     <  2:420397/141‑1 (MQ=255)
        ataAATTTGCAAAACTTATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTg                                                                                                                                  >  2:377771/1‑141 (MQ=255)
                 cAAAACTTATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGaaa                                                                                                                         >  1:20180/1‑141 (MQ=255)
                   aaaCTTATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGAAAATGTTTTTTTATTAGAAATg                                                                                                                       >  1:10542/1‑141 (MQ=255)
                    taCTCATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTCTTAGAAATGt                                                                                                                      >  2:276013/2‑141 (MQ=255)
                    aaCTTATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGACAGTATTTTTACTCCTTAAAATAACATTACATCGTGAATGTTTTTTTATTAGAAATGt                                                                                                                      >  2:962691/1‑141 (MQ=255)
                       ttatttatAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTaaa                                                                                                                   >  2:872544/1‑141 (MQ=255)
                           ttatAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACaa                                                                                                               >  2:613614/1‑141 (MQ=255)
                             ataAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTCAATTCTTTCCAACATAATTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACaaaa                                                                                                             >  2:343157/1‑141 (MQ=255)
                              taAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACaaaaa                                                                                                            >  2:51835/1‑141 (MQ=255)
                              taAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTCCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTCCATCGACAATGTTTTAGTCTTAGAAAAATAGAACaaaaa                                                                                                            >  2:685812/1‑141 (MQ=255)
                                    tttaTTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAat                                                                                                      <  2:462119/138‑1 (MQ=255)
                                    ttAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAat                                                                                                      <  2:173159/141‑1 (MQ=255)
                                        ttATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAACTAGAATATGa                                                                                                  >  2:99870/1‑141 (MQ=255)
                                        ttATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCCACAATGTTTTTGTTTTAGAATTGTAAAACAAAAATAGAATATGa                                                                                                  >  2:742830/1‑141 (MQ=255)
                                         tataAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGATAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGAt                                                                                                 >  2:363101/1‑141 (MQ=255)
                                         tataAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGAt                                                                                                 >  2:193272/1‑141 (MQ=255)
                                          ataAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGAtt                                                                                                <  2:189633/141‑1 (MQ=255)
                                            aaaGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTTAAAATGTAAAACAAAAATAAAATATGCtttt                                                                                              >  1:811482/1‑141 (MQ=255)
                                            aaaGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGAtttt                                                                                              >  1:547103/1‑141 (MQ=255)
                                            aaaGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGAgttt                                                                                              >  1:881156/1‑141 (MQ=255)
                                            aaaGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTATAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATCAGAAATGTAAAACAAAAATAGAATATGAtttt                                                                                              >  1:710954/1‑141 (MQ=255)
                                              aGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGt                                                                                            >  2:599745/1‑141 (MQ=255)
                                                 aTGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATg                                                                                         >  2:840027/1‑141 (MQ=255)
                                                  tGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTCTGa                                                                                        >  1:383598/1‑141 (MQ=255)
                                                    ttGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTCCTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATTTAAAACAAAAATAGAATATGATTTTGTATGAtt                                                                                      >  2:342638/1‑141 (MQ=255)
                                                    ttGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACTTCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGttt                                                                                      >  2:153970/1‑141 (MQ=255)
                                                     tGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTa                                                                                     <  2:375661/141‑1 (MQ=255)
                                                     tGGAAATTGAAATGTTCAATTCTATCCAACATAATTTTCACTTTATAAGTAAGCATTTGTACGCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTa                                                                                     <  2:821069/141‑1 (MQ=255)
                                                      ggAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTAc                                                                                    >  2:803818/1‑141 (MQ=255)
                                                         aattgaaattTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGATAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACaaa                                                                                 <  2:831468/141‑1 (MQ=255)
                                                         aattgaaattTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACaaa                                                                                 <  1:23688/141‑1 (MQ=255)
                                                          attgaaattTTAAATTCTTTCCAACATAGTTGTCACTTTTTAAGTCAGTATTTTTACTCCTTTAACTAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAGCAAAAATAGAATATGATTTTGTATGATTACaaaa                                                                                >  1:408440/1‑141 (MQ=255)
                                                            tgaaattTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGATAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAg                                                                              >  1:109443/1‑141 (MQ=255)
                                                             gaaattTTAAATTCTTTCCAACATACTTTTTATTTTTTTTTTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAgg                                                                             >  2:843502/1‑141 (MQ=255)
                                                                     aaaTTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGAtt                                                                     >  2:977425/1‑141 (MQ=255)
                                                                                   aTACTTTTCACTTTTTAATTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTa                                                       <  2:685596/141‑1 (MQ=255)
                                                                                   aTACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTa                                                       <  1:377771/141‑1 (MQ=255)
                                                                                    tACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGATAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAACAGGAGTTGATTTATTGATTAAACTAt                                                      >  1:652478/1‑141 (MQ=255)
                                                                                     aCTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTAtt                                                     <  1:828399/141‑1 (MQ=255)
                                                                                     aCTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTAtt                                                     <  1:796135/141‑1 (MQ=255)
                                                                                      cTTTTCACTTTTTAAGTCAGTATTTGTCCTCCTTAAACTAACATTACACAGAAAATAATTTGGTAGTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTAttt                                                    <  1:460588/141‑1 (MQ=255)
                                                                                         ttCACTTTTTTAGTCAGTATTTTTACCCCTTAAAATAACATTACACCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTCTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTg                                                 <  1:34913/141‑1 (MQ=255)
                                                                                          tCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTgt                                                >  1:1039563/1‑140 (MQ=255)
                                                                                              tttttAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTaa                                            >  1:861336/1‑141 (MQ=255)
                                                                                                tttAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAGAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGt                                          <  1:191882/141‑1 (MQ=255)
                                                                                                 ttAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTGGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGtt                                         <  1:296197/141‑1 (MQ=255)
                                                                                                    aGTCAGTATTTTTACTCCTTAAAATAACACTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGAAAAAATAAAAAAAGGAGTTGATTTATTGATTAAACGATTTTTGCTTAAGTtata                                      <  2:1024168/141‑1 (MQ=255)
                                                                                                       cAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGACATGTAAAACAAAAATAGAAGATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATCCTg                                   >  2:57189/1‑141 (MQ=255)
                                                                                                       cAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTg                                   >  2:13720/1‑141 (MQ=255)
                                                                                                       cAGTATTTTTACTCCTTAAAATAACATTACATCAACAATGTTTTTGTATTAGAAATGTATAACAAAAATAGAATATGATTTTATATGATGATAAAAAAGAGTAGATTTATTGATTAAACTATTTTTGCTTAAGTTATAATg                                   >  2:700570/1‑141 (MQ=255)
                                                                                                            tttttACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGcac                              <  1:872544/141‑1 (MQ=255)
                                                                                                            tttttACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGcac                              <  1:600518/141‑1 (MQ=255)
                                                                                                            tttttACTCCTTAAAAAAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATTATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGcac                              <  2:592192/141‑1 (MQ=255)
                                                                                                             tattACTCCTTAAAATAACATTACATCGACCATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGcaca                             <  2:673467/139‑1 (MQ=255)
                                                                                                              tttACTCCTTAAAATAACATTACATCGATAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCacac                            <  1:363101/141‑1 (MQ=255)
                                                                                                               ttACTCCTTAAAATAACATTACATCGATAACGTTTTTGAATAAGAAATGTAAGACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACt                           <  2:665978/141‑1 (MQ=255)
                                                                                                                 ccTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACttt                         <  1:90194/140‑1 (MQ=255)
                                                                                                                 aCTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACttt                         <  1:517781/141‑1 (MQ=255)
                                                                                                                 aCTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACttt                         <  1:879032/141‑1 (MQ=255)
                                                                                                                 aCTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACttt                         <  2:139284/141‑1 (MQ=255)
                                                                                                                    acTTAAAATAACATCACATCGACAATGTTTTTGTATTAGCAAGTGAAAACAAAAATACAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCg                      <  2:28657/140‑1 (MQ=255)
                                                                                                                      ttAAAATAACATTCAGTCGACAATGTTTTTGTATTAGAAATGTAAAACAAAATGAGAATATGATTTTGTATGATTAAAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGAc                    <  2:365981/141‑1 (MQ=255)
                                                                                                                      ttAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGAc                    <  1:577767/141‑1 (MQ=255)
                                                                                                                      ttAAAATAACATGACATCGACGTTGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATAATAACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGAc                    <  2:914788/141‑1 (MQ=255)
                                                                                                                           aTAACATTACATCGACAATTTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGACTAAAc               <  1:343157/141‑1 (MQ=255)
                                                                                                                              aCATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGACTAAACaaa            >  2:622495/1‑141 (MQ=255)
                                                                                                                              aCATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTAGGCTAACTTTTCGACTAAACaag            >  1:782121/1‑140 (MQ=255)
                                                                                                                               aaTTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGACTAAACAAAt           <  1:941350/140‑1 (MQ=255)
                                                                                                                                 atACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTAAAAAAAATAAAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGACTAAACAAATtc         <  2:455291/140‑1 (MQ=255)
                                                                                                                                  tACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGCTTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCGAACTTTTAGACTAAACAAAttaa        >  2:816103/1‑139 (MQ=255)
                                                                                                                                    cATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTGGACTAAACAAATtcata      >  1:809810/1‑140 (MQ=255)
                                                                                                                                    cATCGACAATGTTTTAGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGACTAAACAAATtcatc      >  1:787842/1‑141 (MQ=255)
                                                                                                                                        gACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGACTAAACAAATTCATCAAtt  <  1:696685/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AATTCTATATAAATTTGCAAAACTTATTTATAAGGTTTAATTATAAAGTATGTTGGAAATTGAAATTTTAAATTCTTTCCAACATACTTTTCACTTTTTAAGTCAGTATTTTTACTCCTTAAAATAACATTACATCGACAATGTTTTTGTATTAGAAATGTAAAACAAAAATAGAATATGATTTTGTATGATTACAAAAAGGAGTTGATTTATTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGACTAAACAAATTCATCAATT  >  USA300TCH1516_ALE/1265453‑1265729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑