Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,545	G→A	6.4%	T36T (ACC→ACT)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,545	0	G	A	6.4%	179.3 / 3.5	68	T36T (ACC→ACT)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand):  ref base G (28/34);  new base A (2/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-01

ATTCTTTAATCTCACCAATAATAGTAGATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTGAAACGTTTGTGCATTT  >  USA300TCH1516_ALE/1646409‑1646685                                                                                                                                         |                                                                                                                                             aTTCTTTAATCTCACCAATAATAGTAGATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTAc                                                                                                                                          >  1:914246/1‑141 (MQ=255)    cTTTAATTTCACCAATAATAGTAGATTTACGATTAATTATTTCATAATTTGGCGTCTGCTTAGTTGCTTGATCAATATATCTAGAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAg                                                                                                                                       <  2:135591/141‑1 (MQ=255)           ctcACCAATAATAGTCGATCTACGATTAATAATTTCATAATTTGCCGCCTGCTTAGTTGCTTGACCAATATATCTAAAGCCTGTTATCAAATATTGTTTATCTTTAGCTATGCTACGTGCTCCACTCGTACCAGCAAAAcc                                                                                                                                >  2:687835/1‑141 (MQ=255)           ctcACCAATAATAGTAGATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAAcc                                                                                                                                >  2:793447/1‑141 (MQ=255)             cACCAATAATAGTAGATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTg                                                                                                                              <  1:423809/141‑1 (MQ=255)             cACCAATAATAGTAGATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTg                                                                                                                              <  1:440186/141‑1 (MQ=255)              aCCAATAATAGTAGATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGa                                                                                                                             <  1:939870/141‑1 (MQ=255)              aCCAATAATAGTAGATTTACGATTAATAATTTCATAATTTGGCGCCTGCTAAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGGTCCACTGGTACCAGTAAAAccggg                                                                                                                             >  1:31872/1‑138 (MQ=255)              aCCAATAATAGTAGATTTAAACTTAAAATGGACATGATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGa                                                                                                                             >  1:254096/1‑141 (MQ=255)                 aataatAGTAGATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATaa                                                                                                                          <  1:887265/141‑1 (MQ=255)                 aataatAGTAGATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATaa                                                                                                                          <  2:246087/141‑1 (MQ=255)                       agtagATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGATTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATAtct                                                                                                                    >  2:680860/1‑141 (MQ=255)                         tagATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATAGCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATAtcttc                                                                                                                  >  1:640002/1‑141 (MQ=255)                           gATTTACTATTAATAATTTCTTAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTa                                                                                                                <  2:18114/141‑1 (MQ=255)                           gATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGAGCGCCACTGGTACCAGTAAAACCTGATAAATATCTTCTa                                                                                                                >  1:818929/1‑141 (MQ=255)                            aTTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTAt                                                                                                               <  2:572244/141‑1 (MQ=255)                                  gATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAAt                                                                                                         <  1:540306/141‑1 (MQ=255)                                  caTTAATAATTTCATAATTTGACGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAAt                                                                                                         <  2:626495/140‑1 (MQ=255)                                   tctaataaTTTCATAATTGGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCCTTTGTAATc                                                                                                        <  2:917381/139‑1 (MQ=255)                                        taatTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGaaa                                                                                                   >  2:247085/1‑141 (MQ=255)                                         aatTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAACTATCTTCTATTGTAATCCGAAAg                                                                                                  >  2:463015/1‑141 (MQ=255)                                              cATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGa                                                                                             <  1:970285/141‑1 (MQ=255)                                               aTAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATGGTTTATCTTTAGATATGATAAGTGCTCCACTTGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGAt                                                                                            <  1:610395/141‑1 (MQ=255)                                                          ccTGCTTAGTTGCTTGATCAATTTATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTa                                                                                 <  1:87334/141‑1 (MQ=255)                                                           cTGCTTAGTTTCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTaa                                                                                <  2:456706/141‑1 (MQ=255)                                                             gCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAAt                                                                              <  1:693355/141‑1 (MQ=255)                                                              cTTAGTTGCTTGATCAATTTAACTAGAGTCGTTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATg                                                                             <  2:1011639/141‑1 (MQ=255)                                                                  gTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGAAATGCCAAGTGCACCCCTGGTACCAGTAAAACCTGATAAATATCTCCTATTGTAATCCGAAAGAAACATAATCACATCTAAATGtttt                                                                         >  2:294977/1‑141 (MQ=255)                                                                    tGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTg                                                                       >  1:46023/1‑141 (MQ=255)                                                                      cTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATTTTTTTGtt                                                                     >  2:301870/1‑141 (MQ=255)                                                                       ttGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGCTTTTCTGGCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTc                                                                    <  1:244799/141‑1 (MQ=255)                                                                       ttGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACTAGTAAAACCTTATTAATATCTTCTTTTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTc                                                                    <  2:410789/141‑1 (MQ=255)                                                                       ttGATCAATATATCCCACGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTc                                                                    <  1:929386/141‑1 (MQ=255)                                                                          aTCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGTTACTAGTAAACCTTGTTAAATATTTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTaa                                                                 <  1:506323/141‑1 (MQ=255)                                                                           tCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTAGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTaaa                                                                >  2:92387/1‑141 (MQ=255)                                                                           tCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTAGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTaaa                                                                >  2:506972/1‑141 (MQ=255)                                                                            aaacatatCCAAAGCCTGTTATCAAATAATGCTCATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTaaaa                                                               <  1:87762/137‑1 (MQ=255)                                                                                         gTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTtg                                                  <  2:833151/141‑1 (MQ=255)                                                                                            tGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTgtgt                                               >  2:323929/1‑141 (MQ=255)                                                                                               tATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATGTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTACAATACGATGCAGTTGTGTTAt                                            >  2:8501/1‑141 (MQ=255)                                                                                                ttCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTAtt                                           <  1:844277/140‑1 (MQ=255)                                                                                                aTCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTAtt                                           <  2:914246/141‑1 (MQ=255)                                                                                                     ctatCGTTTATCTTTTGATATGATAAGTGCTCCACTGGTAACAGTAAAACCTGCTAAATATCTTCTATTGTAATCAGAAAGAATGCTATTCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCt                                      >  2:399995/2‑141 (MQ=255)                                                                                                     atatTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCt                                      >  1:905086/1‑141 (MQ=255)                                                                                                     atatTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCt                                      <  1:834610/141‑1 (MQ=255)                                                                                                     atatTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCt                                      <  2:299238/141‑1 (MQ=255)                                                                                                         tGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCGTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATc                                  >  1:84075/1‑141 (MQ=255)                                                                                                           tttATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTTTATAATACGATGCACTTGTGTTATTCTGCTCATCtt                                >  2:707998/1‑141 (MQ=255)                                                                                                              aTCTTTAGATATGATAAGTGCTCCACTAGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCttatt                             <  1:92387/141‑1 (MQ=255)                                                                                                              aTCTTTAGATATGATAAGTGCTCCACTAGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCttatt                             <  1:506972/141‑1 (MQ=255)                                                                                                                   tAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTTTGTTATTCTGCTCATCTTATTAcctc                        >  1:389596/1‑141 (MQ=255)                                                                                                                    aGATATGATAAGTGCTCCACCGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTGTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTAcctca                       >  1:745177/1‑140 (MQ=255)                                                                                                                        ttGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGt                   <  1:463750/140‑1 (MQ=255)                                                                                                                        atGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACTTCCTAGt                   >  2:860351/1‑141 (MQ=255)                                                                                                                        atGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGAAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGt                   <  1:1040731/141‑1 (MQ=255)                                                                                                                         tGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTg                  <  2:46023/141‑1 (MQ=255)                                                                                                                         tGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTg                  >  1:8518/1‑141 (MQ=255)                                                                                                                          gATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTACTAAAATACGATGCACTTGTGTTTTTCTGCTCATCTTATTACCTCCTAGCGa                 >  2:859158/1‑141 (MQ=255)                                                                                                                              aGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTGAAACg             >  1:414148/1‑141 (MQ=255)                                                                                                                                  ctcctcTGGTACCAGTAAAACATGATAAATATCTTCTATTGTAATCCGAAAGAATGATAAGCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTACTACCTCCTAGTGAAACGTTtg         <  1:547332/141‑1 (MQ=255)                                                                                                                                   tCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTATGCTCATCTTATTACCTCCTAGTGAAACGTTtgt        >  1:435711/1‑141 (MQ=255)                                                                                                                                    ccACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTGAAACGTTtgtg       <  2:56358/141‑1 (MQ=255)                                                                                                                                    ccACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTGAAACGTTtgtg       >  1:128/1‑141 (MQ=255)                                                                                                                                    ccACTGGTACCAGTAAAACCTCATAAATATCTTCTTTTGTAATCCGAAAGAATGATAATCGCATCTAAATTTTTTTGTTCTAAAATTCGATGCACTTGTATTATTCTGCTTATCTTATTACCTCCTAGTGACACGTTtgtg       <  2:153688/141‑1 (MQ=255)                                                                                                                                     cACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTGAAACGTTTGTGc      <  1:247085/141‑1 (MQ=255)                                                                                                                                      aCTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTGAAACGTTTGTGCa     >  1:1054671/1‑141 (MQ=255)                                                                                                                                        tGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTGCAACGTTTGTGCAtt   <  1:556075/141‑1 (MQ=255)                                                                                                                                        tGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTGAAAGGTTTGTGCAtt   >  1:231900/1‑141 (MQ=255)                                                                                                                                         ggTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTGAAACGTTTGTGCAttt  >  1:701733/1‑141 (MQ=255)                                                                                                                                         |                                                                                                                                             ATTCTTTAATCTCACCAATAATAGTAGATTTACGATTAATAATTTCATAATTTGGCGCCTGCTTAGTTGCTTGATCAATATATCTAAAGTCTGTTATCAAATATTGTTTATCTTTAGATATGATAAGTGCTCCACTGGTACCAGTAAAACCTGATAAATATCTTCTATTGTAATCCGAAAGAATGATAATCGCATCTAAATGTTTTTGTTCTAAAATACGATGCACTTGTGTTATTCTGCTCATCTTATTACCTCCTAGTGAAACGTTTGTGCATTT  >  USA300TCH1516_ALE/1646409‑1646685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑