BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,560	T→C	10.7%	R210R (CGT→CGC)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,560	0	T	C	10.7%	270.4 / 20.2	101	R210R (CGT→CGC)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base T (60/30); new base C (2/9); total (62/39)
Fisher's exact test for biased strand distribution p-value = 2.78e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01

Only 100 of 102 total aligned reads displayed.

TTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGA  >  USA300TCH1516_ALE/111421‑111700

ttAAATTTGGTGCGCAAGATAGAAATTTTAAAGATTAAATGAAGTATAAAGATCGACGTAGATCATATAATTCAGATCTGCC‑AGTTATTCGGCTAAATATCAATTGACTAATGATGATTATAATGTAAAAAAATTACGCaa                                                                                                                                              <  2:446842/141‑1 (MQ=9)

  aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCTATTAACTAATGATGATTATAATGTAAAACAATTACGCaa‑a                                                                                                                                            <  1:158206/141‑1 (MQ=255)

   aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa‑aa                                                                                                                                           >  2:341873/1‑141 (MQ=255)

   aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa‑aa                                                                                                                                           >  2:342013/1‑141 (MQ=255)

      ttttttCTCAATACAGCAACTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAA‑AAGat                                                                                                                                        <  2:654391/141‑1 (MQ=14)

      tttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTAAGAAGCTGGAGATATATCATATAATCCAGAAGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAA‑AAGat                                                                                                                                        <  2:323694/141‑1 (MQ=17)

       ttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAA‑AAGata                                                                                                                                       <  2:915762/141‑1 (MQ=255)

       ttttGCCCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGCTATAGCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTACAACAATTAAGTAA‑AAGata                                                                                                                                       >  2:668287/1‑141 (MQ=21)

          tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAA‑AAGATATG‑a                                                                                                                                   <  2:188817/141‑1 (MQ=255)

            cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACa                                                                                                                                 <  2:798314/141‑1 (MQ=255)

            cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACa                                                                                                                                 >  2:332133/1‑141 (MQ=255)

             gccatatAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTATGTAA‑AAGATATG‑ACAt                                                                                                                                <  1:526655/138‑1 (MQ=255)

             gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACAt                                                                                                                                <  1:758401/141‑1 (MQ=255)

              cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAA‑AAGATATG‑Atata                                                                                                                               <  1:25600/141‑1 (MQ=14)

              cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATa                                                                                                                               <  2:62395/141‑1 (MQ=255)

               aaTATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAA‑AAGATATG‑ATATAc                                                                                                                              <  2:605969/141‑1 (MQ=14)

               aaTATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAA‑AAGATATG‑ATATAc                                                                                                                              <  1:134076/141‑1 (MQ=14)

                 tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGCGGTGCCATGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACca                                                                                                                            <  1:1043017/141‑1 (MQ=255)

                 tagaGCAATTGTAAAGACTTAATGAAGTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACca                                                                                                                            <  2:680394/138‑1 (MQ=255)

                      taaTTTTAAAGATTTAAGGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACaaa                                                                                                                       <  2:80303/140‑1 (MQ=255)

                      cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACaaa                                                                                                                       <  1:921397/141‑1 (MQ=255)

                          tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATaaa                                                                                                                   >  1:811906/1‑141 (MQ=255)

                          tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGAGATG‑ACATACCAACAAATaaa                                                                                                                   >  1:645987/1‑141 (MQ=255)

                          tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAACTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATaaa                                                                                                                   <  1:761654/141‑1 (MQ=255)

                             aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑AAATACCAACAAATAAAgcg                                                                                                                >  2:60217/1‑141 (MQ=255)

                               aGATTTAAGGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGcc                                                                                                              <  2:522904/141‑1 (MQ=255)

                                gagttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCg                                                                                                             <  1:159677/138‑1 (MQ=255)

                                gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATTCCAACAAATAAAGCGCCg                                                                                                             >  1:633993/1‑141 (MQ=255)

                                     aaTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACACATAAAGCGCCGAAGCt                                                                                                        <  1:89250/141‑1 (MQ=255)

                                       tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCtgt                                                                                                      >  2:104189/1‑141 (MQ=255)

                                       tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCtgt                                                                                                      <  2:8740/141‑1 (MQ=255)

                                         aaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGctgctg                                                                                                    >  1:169855/1‑141 (MQ=255)

                                         aaTTAAAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCtgttg                                                                                                    <  1:486636/141‑1 (MQ=255)

                                          aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACTTAA‑AAGATATG‑AAATACCAACAATTAAAGAGACGAGGAtgatgt                                                                                                   >  2:700453/1‑141 (MQ=17)

                                               aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaaa                                                                                              >  2:41846/1‑141 (MQ=255)

                                                aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAg                                                                                             >  2:664874/1‑141 (MQ=255)

                                                aaGATGGAGATATAGCATATAATCCAGAGGTGCCGAGTTATTCAAATCAATAACAAGTAACTAATGATGATTATAATGTACAAAAATTACGTAA‑AAGATATG‑ACATAACTACAAAGAAAGCGCCGAAGCTGTTGTTGAAAg                                                                                             >  2:433744/1‑141 (MQ=17)

                                                    tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                                                                         <  2:886209/141‑1 (MQ=255)

                                                      gagaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAg                                                                                       >  1:214207/1‑141 (MQ=255)

                                                      gagaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAACGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAg                                                                                       >  1:640134/1‑141 (MQ=255)

                                                       agaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                                                                      >  1:253722/1‑141 (MQ=255)

                                                       agaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                                                                      >  1:658539/1‑141 (MQ=255)

                                                        gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                     <  2:87650/141‑1 (MQ=255)

                                                        aatatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAC‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTTAAAGGTACAggg                                                                                     <  2:358382/140‑1 (MQ=255)

                                                          gataaCATATAAGCCAGAGGTGCCGAGTTATTCAGCGCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGaa                                                                                   <  2:434130/140‑1 (MQ=255)

                                                           atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGAGCCGAAGCTGTTGTTGAAAGGTACAGGGAAt                                                                                  >  2:743695/1‑141 (MQ=255)

                                                             atCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATTATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAttt                                                                                <  2:366067/141‑1 (MQ=255)

                                                             atCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAttt                                                                                <  1:433113/141‑1 (MQ=255)

                                                               cATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaa                                                                              >  1:4877/1‑141 (MQ=255)

                                                                    aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                         >  1:602299/1‑141 (MQ=255)

                                                                     aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                        >  1:36867/1‑141 (MQ=255)

                                                                     aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                        >  1:349579/1‑141 (MQ=255)

                                                                      tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGCTGATTAAAATGAAAAACAATTACGTAATACTGAATC‑ACGT‑GGGACGAGAAAATCTCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                       >  2:747909/1‑141 (MQ=9)

                                                                      tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                       >  1:258155/1‑141 (MQ=255)

                                                                       ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGCTATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTATTGAAAGGCACAGGGAATTTAAAAGGTtca                                                                      >  2:55609/1‑141 (MQ=255)

                                                                       ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGAGATGAACACCCCAA‑GAACAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                      >  1:723230/1‑141 (MQ=17)

                                                                        cAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat                                                                     <  1:86518/141‑1 (MQ=255)

                                                                          gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTAAAAAGGTtcatca                                                                   >  2:816715/1‑141 (MQ=255)

                                                                           agGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAg                                                                  >  2:848749/1‑141 (MQ=255)

                                                                             gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                                                                >  1:62889/1‑141 (MQ=255)

                                                                             gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGATTCATCAGtg                                                                >  1:102532/1‑140 (MQ=255)

                                                                             gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATATAAAAGGTTCATCAGtt                                                                >  2:820166/1‑141 (MQ=255)

                                                                             gTGCAGAGTTATTCACGTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                                                                <  2:664541/141‑1 (MQ=255)

                                                                                 cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat                                                            >  2:122270/1‑141 (MQ=255)

                                                                                       aTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaa                                                      >  1:636774/1‑141 (MQ=255)

                                                                                         tCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGATGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaa                                                    >  1:500972/1‑141 (MQ=255)

                                                                                              tCAATATCAATTAACTAATGGTGATTATGATGTAAAACAATTACGTAA‑AAGATAAG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGa                                               >  1:686950/1‑141 (MQ=255)

                                                                                                aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                             <  2:456855/141‑1 (MQ=255)

                                                                                                aaTATCAAATAACTAATGATGATTAAAAAGTAAAACAATTACGTAA‑AAGATATG‑ACATAACAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAATTTTCATAAAAAAAAAAAAAAAAAATTGAAt                                             <  2:527218/141‑1 (MQ=14)

                                                                                                 atatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAttacatt                                            >  2:520956/1‑136 (MQ=255)

                                                                                                 atatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAtt                                            >  1:487549/1‑141 (MQ=255)

                                                                                                 atatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAtt                                            >  2:666362/1‑141 (MQ=255)

                                                                                                    tCACTTAACCAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAc                                         <  1:983471/141‑1 (MQ=255)

                                                                                                    tCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAc                                         <  1:890748/141‑1 (MQ=255)

                                                                                                      aaTTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtt                                       >  2:633784/1‑141 (MQ=255)

                                                                                                         tAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGACGAAGCTGTTGTTGAAAGGTACAGCGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAcatcct                                    >  2:723854/1‑136 (MQ=255)

                                                                                                          aaCTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtttttt                                   >  1:904320/1‑141 (MQ=255)

                                                                                                            cTAATGATGATTATAGTGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAGAGGGAATATAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTag                                 >  1:783248/1‑141 (MQ=255)

                                                                                                            cTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTATag                                 >  2:457213/1‑141 (MQ=255)

                                                                                                            cTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAATTTTTTag                                 >  1:302163/1‑141 (MQ=255)

                                                                                                               atgatgATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAGGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAgaga                              <  1:757372/141‑1 (MQ=255)

                                                                                                                 gatgatTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAAt                            >  1:555283/1‑141 (MQ=255)

                                                                                                                  atgatTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGGTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATa                           <  1:833219/141‑1 (MQ=255)

                                                                                                                     atTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaaa                        >  1:861135/1‑141 (MQ=255)

                                                                                                                      ttATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAATCAGTGGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATGGAATTTGCTTTTTTGTAGAATaaaaa                       >  2:269499/1‑141 (MQ=18)

                                                                                                                      ttATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaaaa                       >  1:975905/1‑141 (MQ=255)

                                                                                                                      ttATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaaaa                       >  1:452919/1‑141 (MQ=255)

                                                                                                                      ttATAATGTAAAACAATTAAGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGGTATAAAAAAATTGAATTTACTTTTTTAGAGAATaaaaa                       >  1:690137/1‑141 (MQ=255)

                                                                                                                         taATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAAGAAATAAAGCGGCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATAAGTTGGATATAAAAAAATTGAATTTACTTTTTTCGAGAATACAAAGGa                    >  2:404340/1‑141 (MQ=17)

                                                                                                                         taATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAACTTGAATGTACTTTTTTAGAGAATAAAAATGa                    >  2:708493/1‑141 (MQ=255)

                                                                                                                           aTGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAACGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaa                  <  1:41846/141‑1 (MQ=255)

                                                                                                                             gTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAat                >  2:358580/1‑141 (MQ=255)

                                                                                                                             gTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAat                >  2:445176/1‑141 (MQ=255)

                                                                                                                               aaaaaaATTACGTAA‑AAGATATG‑AAAAACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAACTGAATTTACTTAATAAAAAAAAAAAAATGAAAatat              <  2:253722/141‑1 (MQ=17)

                                                                                                                                aaaCAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtt             >  2:131006/1‑141 (MQ=255)

                                                                                                                                     aTTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAAGAtttactt        >  2:1014146/1‑141 (MQ=255)

                                                                                                                                     aTTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTAGATATAAAAAAATTGAATTTACTTTTTTAGAGAGTAAAAAGGAAAATAtttactt        >  2:851415/1‑141 (MQ=255)

                                                                                                                                      ttACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttt       >  1:959144/1‑141 (MQ=255)

                                                                                                                                        aCGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATATACTTTTTTAGAGAATAAAAATGAAAATAtttactttac     >  2:443721/1‑141 (MQ=255)

                                                                                                                                           tAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGa  >  1:79029/1‑141 (MQ=255)

TTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAA‑AAGATATG‑ACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGA  >  USA300TCH1516_ALE/111421‑111700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`