breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSNFR_1_52_0_S2417_L001_R1_001.good.fq1,064,668150,091,694100.0%141.0 bases141 bases98.6%
errorsSNFR_1_52_0_S2417_L001_R2_001.good.fq1,064,668150,091,694100.0%141.0 bases141 bases93.8%
total2,129,336300,183,388100.0%141.0 bases141 bases96.2%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionUSA300TCH1516_ALE2,872,91594.72.695.3%Staphylococcus species strain strain.
coveragedistributionCP00073127,041317.85.33.0%Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid
coveragedistributionNC_0124173,1251690.07.61.7%Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid
total2,903,081100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000003571
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500025
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.003

Junction Skew Score Calculation

reference sequencepr(no read start)
USA300TCH1516_ALE0.77342
CP0007310.46670
NC_0124170.11265

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input20:53:42 25 May 201920:54:12 25 May 201930 seconds
Read alignment to reference genome20:54:12 25 May 201920:59:31 25 May 20195 minutes 19 seconds
Preprocessing alignments for candidate junction identification20:59:31 25 May 201921:00:01 25 May 201930 seconds
Preliminary analysis of coverage distribution21:00:01 25 May 201921:01:11 25 May 20191 minute 10 seconds
Identifying junction candidates21:01:11 25 May 201921:01:11 25 May 20190 seconds
Re-alignment to junction candidates21:01:11 25 May 201921:02:18 25 May 20191 minute 7 seconds
Resolving best read alignments21:02:18 25 May 201921:03:02 25 May 201944 seconds
Creating BAM files21:03:02 25 May 201921:04:02 25 May 20191 minute 0 seconds
Tabulating error counts21:04:02 25 May 201921:04:24 25 May 201922 seconds
Re-calibrating base error rates21:04:24 25 May 201921:04:26 25 May 20192 seconds
Examining read alignment evidence21:04:26 25 May 201921:34:07 25 May 201929 minutes 41 seconds
Polymorphism statistics21:34:07 25 May 201921:34:07 25 May 20190 seconds
Output21:34:07 25 May 201921:34:15 25 May 20198 seconds
Total 40 minutes 33 seconds