Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,435,0810TA20.0% 170.1 / 17.2 76N146K (AAT→AAA) dapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (30/30);  new base A (15/0);  total (45/31)
Fisher's exact test for biased strand distribution p-value = 2.06e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTATTGT  >  USA300TCH1516_ALE/1434945‑1435221
                                                                                                                                        |                                                                                                                                            
ttaGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTAc                                                                                                                                          <  1:85382/141‑1 (MQ=255)
     gaACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAAAGTACATGTa                                                                                                                                     >  1:603400/1‑141 (MQ=255)
         ccgCCATTACTGAAGATGGTGCTGGCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAgggg                                                                                                                                 <  1:390728/139‑1 (MQ=255)
         aaGCCATTATTGAATGCGGCGCTGTCGTTATGATGGGCCAAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAgggg                                                                                                                                 <  1:373182/141‑1 (MQ=255)
         aaGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAGAATGTACATGTAgggg                                                                                                                                 <  1:545692/141‑1 (MQ=255)
         aaGCCATGATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAgggg                                                                                                                                 <  2:566622/141‑1 (MQ=255)
           gCCATTATTGAAGATGGTGCTGTCGTTATGATGGTCGCAACCATTAATATTGGCGCAGGCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCt                                                                                                                               <  2:708237/141‑1 (MQ=255)
           gCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCt                                                                                                                               <  1:35303/141‑1 (MQ=255)
           gCCATTACTTAAGTGGGTTCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCt                                                                                                                               <  1:17816/141‑1 (MQ=255)
            ccATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTg                                                                                                                              >  1:555644/1‑141 (MQ=255)
            ccATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTGGAAAAAAAGAACATGTAGGGGCTg                                                                                                                              >  2:15047/1‑141 (MQ=255)
                   tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGt                                                                                                                       >  1:435767/1‑141 (MQ=255)
                   tGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTACCATTAGAAAAAAAGTACATGTAGGGGCTGGCGCTGt                                                                                                                       >  2:781173/1‑141 (MQ=255)
                    ggaGATGGTGGTGTAGGTATGCTGGTCGGCACCATTAATATTGGCGGAGTTGTTGGGGGAGGTTCAATGTTTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGtc                                                                                                                      <  2:700820/139‑2 (MQ=255)
                    gAAGATGGTGCTGTTGTTATGATGGGCGCAACAATTAATATTGGCGCAGTTGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTa                                                                                                                      <  2:589202/141‑1 (MQ=255)
                    gAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTa                                                                                                                      >  1:332506/1‑141 (MQ=255)
                      aGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAACATGTAGGGGCTGGCGCAGTAtt                                                                                                                    >  2:261807/1‑141 (MQ=255)
                      aGATGGTGCTGTCGTTATGATGATCGCAACAATTAATATTGGCGCAGTCGTTGGCGAGGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGGGCTGCAACTGGTAAAAATGTACATGTAGGGGCTGGCGCatgatt                                                                                                                    >  1:28758/1‑136 (MQ=255)
                             gCTGTCGTTATGATGGGCGCAGCAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACATCTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                             <  2:493306/141‑1 (MQ=255)
                             gCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGTGACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                             >  1:718628/1‑141 (MQ=255)
                             gCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAggg                                                                                                             >  1:729909/1‑140 (MQ=255)
                             gCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAATAAAAATATAACTGTAGGGGCGGGGGCAGTATTAGCAggg                                                                                                             >  2:315898/1‑140 (MQ=255)
                             gCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAATATACATGCAGGGGCTGGCGCAGTATTAGCAGgt                                                                                                             >  2:292919/1‑141 (MQ=255)
                                gTCGTTATGATGGGCGGAACAGTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGAGCTGGCGCAGTATTAACAGGagtg                                                                                                          >  1:471089/1‑141 (MQ=255)
                                gTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGCTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGtgtg                                                                                                          >  1:657685/1‑141 (MQ=255)
                                  tgtttagataGGCGGAAAAATTAATTTTGGCGTAGTCGTTGGTGAAGGGACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGTAGTATTAGCAGGTGTGAt                                                                                                        <  2:881499/135‑1 (MQ=255)
                                  cGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGAt                                                                                                        <  1:313588/141‑1 (MQ=255)
                                  cGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGAt                                                                                                        <  2:383055/141‑1 (MQ=255)
                                          tgGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCACTAGAAAAAAAATACATGTAGAGGCACGCGACGCATCAGCAGGGAAGATTGAAcccc                                                                                                >  2:487767/1‑141 (MQ=255)
                                             gcgcAACAATTAATATTGGCGCTGCAGTAAACGAATGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTa                                                                                             >  1:914437/1‑141 (MQ=255)
                                             gcgcAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGGACATGTAGGGGCTGGCGCAGTATTAGCAGGGGTGATTGAACCCCCTa                                                                                             >  1:241143/1‑141 (MQ=255)
                                               gcAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGt                                                                                           >  1:148961/1‑141 (MQ=255)
                                               gcAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGt                                                                                           >  1:394144/1‑141 (MQ=255)
                                                 aacaaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTACCACTGAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGtcc                                                                                         >  2:394826/1‑139 (MQ=255)
                                                 aacaaTTAATATGTGCGCAGTCGTTGGCGGAAGTACAATGATTGCTTTCTTAGCTACCCTCGGTGGTCGTGCTACATCTGGTAAAAATGTCCCTGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGc                                                                                         <  1:298689/141‑1 (MQ=255)
                                                  acaaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGGTATCATTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACTCCCTGGTGCt                                                                                        >  2:876233/1‑141 (MQ=255)
                                                  acaaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACACGCTGGTGCt                                                                                        >  2:641357/1‑141 (MQ=255)
                                                  acaaTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTACCACTAAAAAAAAAGTACATGTAGGGGCTGGCGCAGTATGAGCAGGTGTGATTGAACCCCCTAGTGCt                                                                                        >  2:557232/1‑141 (MQ=255)
                                                   aaaTTAATAGTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTCCTCTCGGTGGGCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCtt                                                                                       <  2:667909/140‑1 (MQ=255)
                                                    aaTTAATATTGGCGTAGTCGTTGGCGAAGATACAATGATTGATATGAATGCTACTCTAGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTCGTGCTTc                                                                                      <  2:565684/141‑1 (MQ=255)
                                                               gcgcAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATaa                                                                           <  1:555958/141‑1 (MQ=255)
                                                                  cAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCg                                                                        >  1:725787/1‑141 (MQ=255)
                                                                  cAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGGTATAATCg                                                                        >  1:120202/1‑141 (MQ=255)
                                                                  cAGACGTTGGGCAAGGTAAAAAGAATGATATGAATGTGACTCTCGGTGGTCGAGCAACAACTGGTAAAAATGTACATGTCGTGGCTGGCTCAGTATTAGCAGGTGTGATTGATCCGCCTAGTGCTTCACCGGTTATAATCg                                                                        <  2:148474/141‑1 (MQ=255)
                                                                    gTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAg                                                                      <  1:320844/141‑1 (MQ=255)
                                                                     tCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAgg                                                                     >  1:275493/1‑141 (MQ=255)
                                                                     gtgTTGGAGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAgg                                                                     <  2:764957/139‑1 (MQ=255)
                                                                      cGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAGAAAAAAAGTACATGTAGGGGCTGGCGCAGTATTAGCAGGGGTGATTAGAACCCCAAGAGCTTCACCGGTTATAAACGAGga                                                                    >  2:484608/1‑141 (MQ=255)
                                                                       gTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACCACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGGGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGGGgat                                                                   >  1:137784/1‑141 (MQ=255)
                                                                       gTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGgat                                                                   >  1:227571/1‑141 (MQ=255)
                                                                        ttGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGGGgatg                                                                  >  1:572666/1‑141 (MQ=255)
                                                                        ttGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTGACCGGTTATAATCGAGgatg                                                                  >  2:242650/1‑141 (MQ=255)
                                                                         tGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTACAATTGGTAAAAAAGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCCCCGGGTATAATCGGGgatga                                                                 >  2:82009/1‑141 (MQ=255)
                                                                               agttagaaTGGTTGAAATGGATGCCAATCTGGGTGGTCGTGCTACAACTGGTAAAAATGTACATGCAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTAtt                                                           <  2:221106/135‑1 (MQ=255)
                                                                               aGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTAtt                                                           <  1:232433/141‑1 (MQ=255)
                                                                               aGGTACAATGATTGATATGAATGCTACTCTCGGTGGGCGTGCTACAACTGGTAAACATGTCAGTGTAGTGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTAtt                                                           <  1:906527/141‑1 (MQ=255)
                                                                                      aTGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAAGGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCTAGGATGATGTATTAATCGGt                                                    <  1:277573/141‑1 (MQ=255)
                                                                                              tatGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATAGGTGCCAACGc                                            >  2:138836/1‑141 (MQ=255)
                                                                                              tatGAATGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAGAACATGTAGGGACTGGCGCAGGATTAGCAGGTGTGATTGAACCCCCTAGTGCTTAATCGGTTATAATAGAGGATGATGAGTTAATAGGGGCAAATGc                                            >  2:459050/1‑141 (MQ=255)
                                                                                                  aaTGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGtt                                        >  1:584587/1‑141 (MQ=255)
                                                                                                  aaTGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAGTAAATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGGGCTTCACCGGTTATAATCGAGGAGAAGGGATTAATCGGTGCaaagcacgtt                                        >  2:861927/1‑134 (MQ=255)
                                                                                                    tGCTACTCTCGGTGGTCGCCGTATCATTAAAAAAAAAATACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGTTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTAt                                      >  2:903055/1‑141 (MQ=255)
                                                                                                         ctctCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTaa                                 >  1:530426/1‑140 (MQ=255)
                                                                                                            ccGGTGGTCGGGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAg                              <  1:82009/140‑1 (MQ=255)
                                                                                                              ggtggtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAAGCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGGATTAATGGGTGCAATTGCAGTTGTTTTCTAAGgt                            >  1:505726/1‑141 (MQ=255)
                                                                                                                  gtCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCCGGCGCAGCATGAGCAGGTGTGATTGAACCCCAACATTTGTCAACGGGTATGATCCAGGAGGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTAc                        <  1:216151/141‑1 (MQ=255)
                                                                                                                  gccGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGGGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTAc                        <  1:34760/139‑1 (MQ=255)
                                                                                                                      tGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACgtgt                    >  2:716386/1‑141 (MQ=255)
                                                                                                                      tGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGGGTACgtgg                    >  2:576164/1‑140 (MQ=255)
                                                                                                                         tACAAATGGTAAAAATGTACCTGTAGGTGCTGGCGCTGTAGTAGCAGGTTTCATTGAATCCCCTAGGGCTTCGTCGGTTGTTATCGTGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGCAGGTGTACgtggtgc                 <  2:533492/141‑2 (MQ=255)
                                                                                                                            aaCTGTTAAAAATGTACATGGAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTaa              <  2:674384/141‑1 (MQ=255)
                                                                                                                            aaCTGGTAAAAATGTACATGTAGGGGTTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTaa              <  1:524994/141‑1 (MQ=255)
                                                                                                                                  tAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGc        >  1:527921/1‑141 (MQ=255)
                                                                                                                                  tAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCGAGTGCTTCACCGGTTATAATCGAGCATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGc        >  1:595412/1‑141 (MQ=255)
                                                                                                                                   aaaaaTGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGTTGTAGGAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCt       >  1:905408/1‑141 (MQ=255)
                                                                                                                                      aaTGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGGTGGTAAAGGTGCTAtt    >  2:475276/1‑141 (MQ=255)
                                                                                                                                        tGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTAttgt  >  2:159763/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                            
TTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTATTGT  >  USA300TCH1516_ALE/1434945‑1435221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑