Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,963,768	C→G	V365L (GTA→CTA)	hemY ←	Protoporphyrinogen oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,963,768	0	C	G	100.0%	284.0 / NA	84	V365L (GTA→CTA)	hemY	Protoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (44/40);  total (44/40)

CTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGT  >  USA300TCH1516_ALE/1963632‑1963902                                                                                                                                         |                                                                                                                                       cTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATAc                                                                                                                                    >  1:381929/1‑141 (MQ=255)   tttCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGc                                                                                                                                  >  1:2028/1‑141 (MQ=255)   tttCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGc                                                                                                                                  <  2:500332/141‑1 (MQ=255)        gCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGaa                                                                                                                             >  2:803446/1‑141 (MQ=255)         cAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATAATTAAATTGAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTNTACCTAGATACGCACGAAt                                                                                                                            >  1:357449/1‑141 (MQ=255)           aaggATGGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGCCTTAAATCTCTACGTACAATCGATACTAATTCATGATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCa                                                                                                                          <  2:819989/138‑1 (MQ=255)           aaCGACTGACAATTGTAAATTCAGGACCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCCGTATGATCATCAAGCACAGTATCACCTTGTTTACCTAGATACGCACGAATCa                                                                                                                          <  1:591134/141‑1 (MQ=255)              gATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACCGTATCACCTGGTTTACCTAGATACGCACGAATCaaaa                                                                                                                       >  2:134510/1‑141 (MQ=255)                 tGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTACTTCATTATCTGTATGATCACGAACCCTCTTATAATCTGGTGTACCTAGATACGCACGAATCAAAtcct                                                                                                                    >  2:646788/1‑141 (MQ=255)                 tGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGTTTTACCTCGATACGCCCGACTCAAAacct                                                                                                                    >  2:186163/1‑141 (MQ=255)                 tGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacct                                                                                                                    <  1:386795/141‑1 (MQ=255)                 tGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacct                                                                                                                    <  2:297722/141‑1 (MQ=255)                 tGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacct                                                                                                                    <  1:754952/141‑1 (MQ=255)                 tGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacct                                                                                                                    >  1:264925/1‑141 (MQ=255)                   aCAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAAGCACAGTATCACCTGGTTTACCTAGGTACGGAGGCATCGAAaccttc                                                                                                                  >  2:112554/1‑140 (MQ=255)                   aCAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttc                                                                                                                  >  1:536433/1‑140 (MQ=255)                    cAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttac                                                                                                                 <  2:430169/141‑1 (MQ=255)                     aaTTGTAAATGCATTATCACCTTTAAATGTCATCATTTGACTTAAATTCCTACGTGCAAGCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGAGACGCACGAATCAAAaccttacc                                                                                                                <  2:22753/141‑1 (MQ=255)                      aTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct                                                                                                               >  2:527789/1‑141 (MQ=255)                      aTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct                                                                                                               >  2:547108/1‑141 (MQ=255)                      aTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct                                                                                                               <  1:795505/141‑1 (MQ=255)                       ttGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacctt                                                                                                              <  1:518985/141‑1 (MQ=255)                       ttGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacctt                                                                                                              <  2:139037/141‑1 (MQ=255)                         gTAAATCCAGCATCACCTGTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACACTCACACCCGGTTTACCTTGATACACAAGCACCGAAACCTTACCTTcc                                                                                                            >  2:441133/1‑141 (MQ=255)                           aaaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCgg                                                                                                          <  2:625897/141‑1 (MQ=255)                            atttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGgt                                                                                                         <  2:432524/139‑1 (MQ=255)                                 aGGATCACCTTTAAATCTCATCATTTCACCTAAATCTCCCCGCACACCCGATACTAATTCATTATCTGTATGACCAGCAACCACAGTAGCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTgtagt                                                                                                    <  2:870496/141‑1 (MQ=255)                                  ggATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTgtagta                                                                                                   <  1:363227/141‑1 (MQ=255)                                      cACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTATTAAATg                                                                                               >  2:167595/1‑141 (MQ=255)                                       ccccttAAATTTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATgg                                                                                              <  2:412340/137‑1 (MQ=255)                                       aCCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATgg                                                                                              <  2:45711/141‑1 (MQ=255)                                          tttAAATGTCATCATTTGACTTAAATCTCTACGTACATTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCa                                                                                           <  2:71389/141‑1 (MQ=255)                                                  tcatcaTTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATTTGTATGATCATCAACCACAGTATCACCTGGGTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCg                                                                                   <  2:554918/141‑1 (MQ=255)                                                   catcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa                                                                                  <  1:347843/141‑1 (MQ=255)                                                     tcatTTGACTTAACTCTATACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATg                                                                                <  2:898417/141‑1 (MQ=255)                                                     tcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATg                                                                                <  1:283534/141‑1 (MQ=255)                                                     tcatTTGACTTAAATCTCTACGTACAATCGAGACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAtttttccactc                                                                                <  2:465023/141‑7 (MQ=255)                                                      catTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTTTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTCGTAACGGGACATTTTTTCGATGt                                                                               >  1:118692/1‑141 (MQ=255)                                                      catTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGCTGt                                                                               >  1:822654/1‑141 (MQ=255)                                                      catTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGt                                                                               >  1:696318/1‑141 (MQ=255)                                                       atTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATGAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAACACCTTACCTTACGTCGTAGTAAATGGTCATTTTTTCTATATc                                                                              >  1:391939/1‑141 (MQ=255)                                                             cTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTGTCGATGTCCAAGTa                                                                        <  1:602602/141‑1 (MQ=255)                                                             cTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGGTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCCTTTTTTCGATGTCCAAGTa                                                                        >  1:885546/1‑141 (MQ=255)                                                                aaaTCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACAt                                                                     >  2:283821/1‑141 (MQ=255)                                                                  aTCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGc                                                                   <  1:891576/141‑1 (MQ=255)                                                                     ttcccGTACACCCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGt                                                                <  1:823717/137‑1 (MQ=255)                                                                      ctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTa                                                               <  1:94446/141‑1 (MQ=255)                                                                       tACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTCCATGCGGTaa                                                              >  2:470523/1‑141 (MQ=255)                                                                       tACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTaa                                                              >  1:328126/1‑141 (MQ=255)                                                                         cGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCGTTTTTTCGATGTCCAAGGACATGCGGTACTg                                                            >  1:331173/1‑141 (MQ=255)                                                                            aCAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCt                                                         <  1:393328/141‑1 (MQ=255)                                                                                 cGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTGTc                                                    >  2:222675/1‑141 (MQ=255)                                                                                 cGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATc                                                    >  2:5707/1‑141 (MQ=255)                                                                                   aTACTAATTCATGATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCAc                                                  <  2:517746/141‑1 (MQ=255)                                                                                    tACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACt                                                 <  1:832190/141‑1 (MQ=255)                                                                                    caCTAATTCATCATCTGTACGATCATCAACCCCAGTATCACCTGGTTTACCCAGATCCGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACt                                                 <  1:84646/140‑1 (MQ=255)                                                                                           tCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGAGGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTtctc                                          >  2:343948/1‑141 (MQ=255)                                                                                            cATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCg                                         <  1:805160/141‑1 (MQ=255)                                                                                               tATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAACACCTTACCTTCCAGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGAAAATGGCTGTCCCATTCACTCTCGCat                                      >  2:139774/1‑140 (MQ=255)                                                                                               tATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCaa                                      >  2:245141/1‑141 (MQ=255)                                                                                                aTCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAt                                     <  1:319678/141‑1 (MQ=255)                                                                                                aTCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAt                                     >  1:286590/1‑141 (MQ=255)                                                                                                 tCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAtt                                    >  2:128183/1‑141 (MQ=255)                                                                                                  cTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATATCTGTATCACTCGTTCTCGCAATTa                                   >  1:527010/1‑141 (MQ=255)                                                                                                   tGTATGATCATCAACCACAGCCACGCCGTGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTAc                                  >  2:198177/1‑141 (MQ=255)                                                                                                     tATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGa                                >  1:771890/1‑141 (MQ=255)                                                                                                     tATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGCATAACCCGTTCTCGCAATTACgg                                >  1:247912/1‑140 (MQ=255)                                                                                                       tGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATTCTGTAAGGTCTGTATCACTAGTTCTCACAATTACGacg                              >  2:782794/1‑139 (MQ=255)                                                                                                       tGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAg                              <  1:470523/141‑1 (MQ=255)                                                                                                       tGAGCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAg                              >  2:465323/1‑141 (MQ=255)                                                                                                           catcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAg                          >  1:809571/1‑141 (MQ=255)                                                                                                           catcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGCAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAg                          >  1:809588/1‑141 (MQ=255)                                                                                                            atcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGt                         >  2:281234/1‑141 (MQ=255)                                                                                                                aCCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCCCTCGTTCTCGCAATTACGAAGCCAGTACca                     >  2:396357/1‑141 (MQ=255)                                                                                                                aCCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCCATTACGCAGCCAGTACca                     >  2:157183/1‑141 (MQ=255)                                                                                                                 ccACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcat                    >  1:384106/1‑141 (MQ=255)                                                                                                                   acaGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatca                  >  1:325240/1‑141 (MQ=255)                                                                                                                      ttATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATgg               <  2:381929/140‑1 (MQ=255)                                                                                                                       tATCCCCTGCTTTACTTAGATACTCAGGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATAACTCGTTCTCGCAATTACGAAGCCAGTACCATCATggg              <  2:357449/141‑1 (MQ=255)                                                                                                                       tATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATggg              <  1:469341/141‑1 (MQ=255)                                                                                                                         tCACCTGGTTTACCTAGCTACGCCCTTTTATTTTCCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATTCGGTAATGTCTGTATCACTCGTTCTCGCAATTACCAAGCCCATAACATCATGGGTa            >  2:889648/1‑141 (MQ=255)                                                                                                                               ggTTTACCTAGATACGCACGAATCAAACCCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCa      <  1:159058/141‑1 (MQ=255)                                                                                                                                gTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCaa     <  1:5707/141‑1 (MQ=255)                                                                                                                                   tACCTAGATACGCACGAAGCAAAACCTTACCTTCCGGTGGAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGt  <  2:2028/141‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGT  >  USA300TCH1516_ALE/1963632‑1963902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑